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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 18870R-2 
 Symbol CG18870  Full Name CG18870 
 CG No CG18870  Old CG No CG18870 
 Synonyms unnamed, anon-57Fa, CG18870 
 Accession No (Link to NCBI) NM_144447.1 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Ohhara Y, Nakamura A, Kato Y, Yamakawa-Kobayashi K.
Chaperonin TRiC/CCT supports mitotic exit and entry into endocycle in Drosophila.
PLoS Genet (2019) 15(4) e1008121 [ PubMed ID = 31034473 ] [ RRC reference ]

Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgATCGGATTTGGGTCACTCTGG 
 Primer Seq. 3' 
aatctagaggtaccGGATCGCCTAGTATATGACGC 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 ATCGGATTTG GGTCACTCTG GCCACTGgcC ATGATGGTGG CCTTTTGCAC TGCCTACAAG 
0061 AACGTTCGTC TCCAGGACAA CATGTGCTCG GACAAACTGT TCTACACGCT GGCNCAAGCC 
0121 GTTCCGTGGC GATACCGACT GTGCGGCTGG AGTTCGAGGA CACCTCCGGA GCGATCGTGA 
0181 CGCAGATGTG GAATTTGACA CTGCCCCACG GCCATACGGC CAACAAGATT AAGTACGATT 
0241 GCCAGTTTAG GGTGGTGACC AGCGAGCNGA CCGCgACGCG GCATTTACAC TATCATTACG 
0301 CGGCTTAAGT TTCGGCGCGA CCCCGTTTCC AAGGAGTGCA TCGACTACAT TCAGTTCAGC 
0361 AGCGGCAATC GCTCGCCCCA GCGAACGCAT TTGCACGGGA CATCTCCATT GATGGNCCGG 
0421 CGGGACGTCT GATCTTCGAT CAGCGTGATC GCGACGTCAA CGTGCACATA TTTATCGATC 
0481 GATCGCGTCA TATACTANGC GATCC 
 in silico PCR Fragment 
0001 ATCGGATTTG GGTCACTCTG GCCACTGGCC ATGATGGTGG CCTTTTGCAC TGCCTACAAG 
0061 AACGTTCGTC TCCAGGACAA CATGTGCTCG GACAAACTGT TCTACACGCT GGC-CAAGCC 
0121 GTTCCGTGGC GAT-CCGACT GTGCGGCTGG AGTTCGAGGA CACCTCCGGA GCGATCGTGA 
0181 CGCAGATGTG GAATTTGACA CTGCCCCACG GCCATACGGC CAACAAGATT AAGTACGATT 
0241 GCCAGTTTAG GGTGGTGACC AGCGAGC-GA CCGCGACGCG GCATTTACAC TATCATTACG 
0301 CGGCTTAAGT TTCGGCGCGA CCCCGTTTCC AAGGAGTGCA TCGACTACAT TCAGTTCAGC 
0361 AGCGGCAATC GCTCG-CCCA GCGAACGCAT TTGCAC-GGA CATCTCCATT GATGGCCCGG 
0421 CGGGACGTCT GATCTTCGAT CAGCGTGATC GCGACGTCAA CGTGCACATA TTTATCGATC 
0481 GATCGCGTCA TATACTAGGC GATCC 
 Assemble Data 

18870R-2.IR_full       1   ATCGGATTTGGGTCACTCTGGCCACTGGCCATGATGGTGGCCTTTTGCACTGCCTACAAG 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18870R-2.in silico     1   ATCGGATTTGGGTCACTCTGGCCACTGGCCATGATGGTGGCCTTTTGCACTGCCTACAAG 60


18870R-2.IR_full       61  AACGTTCGTCTCCAGGACAACATGTGCTCGGACAAACTGTTCTACACGCTGGCNCAAGCC 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||| ||||||
18870R-2.in silico     61  AACGTTCGTCTCCAGGACAACATGTGCTCGGACAAACTGTTCTACACGCTGGC-CAAGCC 120


18870R-2.IR_full       121 GTTCCGTGGCGATACCGACTGTGCGGCTGGAGTTCGAGGACACCTCCGGAGCGATCGTGA 180
                           ||||||||||||| ||||||||||||||||||||||||||||||||||||||||||||||
18870R-2.in silico     121 GTTCCGTGGCGAT-CCGACTGTGCGGCTGGAGTTCGAGGACACCTCCGGAGCGATCGTGA 180


18870R-2.IR_full       181 CGCAGATGTGGAATTTGACACTGCCCCACGGCCATACGGCCAACAAGATTAAGTACGATT 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18870R-2.in silico     181 CGCAGATGTGGAATTTGACACTGCCCCACGGCCATACGGCCAACAAGATTAAGTACGATT 240


18870R-2.IR_full       241 GCCAGTTTAGGGTGGTGACCAGCGAGCNGACCGCGACGCGGCATTTACACTATCATTACG 300
                           ||||||||||||||||||||||||||| ||||||||||||||||||||||||||||||||
18870R-2.in silico     241 GCCAGTTTAGGGTGGTGACCAGCGAGC-GACCGCGACGCGGCATTTACACTATCATTACG 300


18870R-2.IR_full       301 CGGCTTAAGTTTCGGCGCGACCCCGTTTCCAAGGAGTGCATCGACTACATTCAGTTCAGC 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18870R-2.in silico     301 CGGCTTAAGTTTCGGCGCGACCCCGTTTCCAAGGAGTGCATCGACTACATTCAGTTCAGC 360


18870R-2.IR_full       361 AGCGGCAATCGCTCGCCCCAGCGAACGCATTTGCACGGGACATCTCCATTGATGGNCCGG 420
                           ||||||||||||||| |||||||||||||||||||| |||||||||||||||||| ||||
18870R-2.in silico     361 AGCGGCAATCGCTCG-CCCAGCGAACGCATTTGCAC-GGACATCTCCATTGATGGCCCGG 420


18870R-2.IR_full       421 CGGGACGTCTGATCTTCGATCAGCGTGATCGCGACGTCAACGTGCACATATTTATCGATC 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18870R-2.in silico     421 CGGGACGTCTGATCTTCGATCAGCGTGATCGCGACGTCAACGTGCACATATTTATCGATC 480


18870R-2.IR_full       481 GATCGCGTCATATACTANGCGATCC 505
                           ||||||||||||||||| |||||||
18870R-2.in silico     481 GATCGCGTCATATACTAGGCGATCC 505



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_144447.1  CG18870-RA (CG18870), mRNA 
0   0  3  4  3  NM_132200.1  CG1531-RB (CG1531), mRNA 
0   0  2  4  3  NM_138091.2  CG3511-RA (CG3511), mRNA 
0   0  2  2  3  NM_170653.1  CG6643-RB, transcript variant B (CG6643), mRNA 
0   0  2  2  3  NM_170652.1  CG6643-RA, transcript variant A (CG6643), mRNA 
0   0  2  2  2  NM_057400.3  CG6993-RA (ss), mRNA 
0   0  2  2  2  NM_137727.1  CG10505-RA (CG10505), mRNA 
0   0  1  6  6  NM_164668.1  CG9075-RA, transcript variant A (eIF-4a), mRNA 
0   0  1  6  6  NM_057247.3  CG9075-RC, transcript variant C (eIF-4a), mRNA 
0   0  1  6  6  NM_164669.1  CG9075-RB, transcript variant B (eIF-4a), mRNA 
0   0  1  6  6  NM_164670.1  CG9075-RD, transcript variant D (eIF-4a), mRNA 
0   0  1  5  3  NM_176184.2  CG18250-RC, transcript variant C (Dg), mRNA 
0   0  1  5  3  NM_079032.2  CG18250-RA, transcript variant A (Dg), mRNA 
0   0  1  5  3  NM_166138.3  CG18250-RB, transcript variant B (Dg), mRNA 
0   0  1  4  7  NM_057442.3  CG15288-RA, transcript variant A (wb), mRNA 
0   0  1  4  7  NM_165086.1  CG15288-RB, transcript variant B (wb), mRNA 
0   0  1  3  4  NM_079188.1  CG1132-RA (fd64A), mRNA 
0   0  1  3  4  NM_167182.1  CG2194-RC, transcript variant C (Reg-3), mRNA 
0   0  1  3  4  NM_132310.2  CG2194-RB, transcript variant B (Reg-3), mRNA 
0   0  1  2  5  NM_134671.1  CG11592-RA (CG11592), mRNA 
0   0  1  2  3  NM_142221.1  CG18516-RA (CG18516), mRNA 
0   0  0  6  3  NM_135655.2  CG4788-RA (CG4788), mRNA 
0   0  0  5  5  NM_170360.1  CG4976-RA (Mes-4), mRNA 
0   0  0  5  4  NM_169450.1  CG14741-RA (CG14741), mRNA 
0   0  0  5  4  NM_135176.2  CG9506-RA (slam), mRNA 
0   0  0  5  3  NM_139676.1  CG13708-RA (CG13708), mRNA 
0   0  0  4  8  NM_142783.2  CG13849-RA (Nop56), mRNA 
0   0  0  4  8  NM_140405.1  CG10710-RA (CG10710), mRNA 
0   0  0  4  5  NM_176579.1  CG33203-RC (CG33203), mRNA 
0   0  0  4  3  NM_137572.2  CG10062-RA (CG10062), mRNA 

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