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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 18815R-1 
 Symbol CG18815  Full Name CG18815 
 CG No CG18815  Old CG No CG18815 
 Synonyms anon-EST:Posey232, CG18815 
 Accession No (Link to NCBI) NM_144417.2 
 Inserted Chr. ll 
 Insertional Mutation  lethal 
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgATTGTCGAGGCCACGGTCAAG 
 Primer Seq. 3' 
aatctagaggtaccAATTTGTACGGCACCACGGGA 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 ATTGTCGAGG CCACGGTCAA GCAAACCGCC ACGCTGATCT TTATGCACGG CCTGGGTGAT 
0061 ACGGGTCATG GCTGGAGCAG TGCCCTAGCC GCCATTCGCC CACCTTTCAT GAAAGTCATA 
0121 TGCCCCACGG CGCCCACACA GCCCGTTTCG CTGAACGCCG GCTTCCGCAT GCCGTCGTGG 
0181 TTTGACCTGA AGACGCTGGA CATCGGTGGG CCCGAGGACG AGCCGGGCAT CCAGTCGGCG 
0241 CGTGATAGTG TGCACGGTAT GATCCAGAAG GAGATTAGCG CTGGGATTCC GGCCAATCGC 
0301 ATTGTCCTGG GCGGATTNCT CGCAGGGCGG CAGCTTTGGC ACTGTACTCG GCACTCACGT 
0361 ACGATNCAgc CGCTGGCCGG CGTGGTGGCC CTATCCTGTN GGCTGCCGCT GCACAAGCAG 
0421 TTCCCAGGCG CCAAGGTGAA CAGCGATGAT GTGCCGATTT TCCAGGCGCA TGGCGACTAC 
0481 GATCCCGTGG TGCCGTACAA ATT 
 in silico PCR Fragment 
0001 ATTGTCGAGG CCACGGTCAA GCAAACCGCC ACGCTGATCT TCATGCACGG CCTGGGTGAT 
0061 ACGGGTCATG GCTGGAGCAG TGCCCTAGCC GCCATCCGCC CACCTTTCAT GAAAGTCATC 
0121 TGCCCCACGG CGCCCACACA GCCCGTTTCG CTGAACGCCG GCTTCCGCAT GCCGTCGTGG 
0181 TTTGACCTGA AGACGCTGGA CATCGGTGGG CCCGAGGACG AGCCGGGCAT CCAGTCGGCG 
0241 CGTGATAGTG TGCACGGTAT GATCCAGAAG GAGATTAGCG CTGGGATTCC GGCCAATCGC 
0301 ATTGTCCTGG GCGGATT-CT CGCAGGGCGG -AGCCTTGGC ACTGTACTCG GCACTCACGT 
0361 ACGAT-CAGC CGCTGGCCGG CGTGGTGGCC CTATCCTGTT GGCTGCCGCT GCACAAGCAG 
0421 TTCCCAGGCG CCAAGGTGAA CAGCGATGAT GTGCCGATTT TCCAGGCGCA TGGCGACTAC 
0481 GATCCCGTGG TGCCGTACAA ATT 
 Assemble Data 

18815R-1.IR_full       1   ATTGTCGAGGCCACGGTCAAGCAAACCGCCACGCTGATCTTTATGCACGGCCTGGGTGAT 60
                           ||||||||||||||||||||||||||||||||||||||||| ||||||||||||||||||
18815R-1.in silico     1   ATTGTCGAGGCCACGGTCAAGCAAACCGCCACGCTGATCTTCATGCACGGCCTGGGTGAT 60


18815R-1.IR_full       61  ACGGGTCATGGCTGGAGCAGTGCCCTAGCCGCCATTCGCCCACCTTTCATGAAAGTCATA 120
                           ||||||||||||||||||||||||||||||||||| ||||||||||||||||||||||| 
18815R-1.in silico     61  ACGGGTCATGGCTGGAGCAGTGCCCTAGCCGCCATCCGCCCACCTTTCATGAAAGTCATC 120


18815R-1.IR_full       121 TGCCCCACGGCGCCCACACAGCCCGTTTCGCTGAACGCCGGCTTCCGCATGCCGTCGTGG 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18815R-1.in silico     121 TGCCCCACGGCGCCCACACAGCCCGTTTCGCTGAACGCCGGCTTCCGCATGCCGTCGTGG 180


18815R-1.IR_full       181 TTTGACCTGAAGACGCTGGACATCGGTGGGCCCGAGGACGAGCCGGGCATCCAGTCGGCG 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18815R-1.in silico     181 TTTGACCTGAAGACGCTGGACATCGGTGGGCCCGAGGACGAGCCGGGCATCCAGTCGGCG 240


18815R-1.IR_full       241 CGTGATAGTGTGCACGGTATGATCCAGAAGGAGATTAGCGCTGGGATTCCGGCCAATCGC 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18815R-1.in silico     241 CGTGATAGTGTGCACGGTATGATCCAGAAGGAGATTAGCGCTGGGATTCCGGCCAATCGC 300


18815R-1.IR_full       301 ATTGTCCTGGGCGGATTNCTCGCAGGGCGGCAGCTTTGGCACTGTACTCGGCACTCACGT 360
                           ||||||||||||||||| |||||||||||| ||| |||||||||||||||||||||||||
18815R-1.in silico     301 ATTGTCCTGGGCGGATT-CTCGCAGGGCGG-AGCCTTGGCACTGTACTCGGCACTCACGT 360


18815R-1.IR_full       361 ACGATNCAGCCGCTGGCCGGCGTGGTGGCCCTATCCTGTNGGCTGCCGCTGCACAAGCAG 420
                           ||||| ||||||||||||||||||||||||||||||||| ||||||||||||||||||||
18815R-1.in silico     361 ACGAT-CAGCCGCTGGCCGGCGTGGTGGCCCTATCCTGTTGGCTGCCGCTGCACAAGCAG 420


18815R-1.IR_full       421 TTCCCAGGCGCCAAGGTGAACAGCGATGATGTGCCGATTTTCCAGGCGCATGGCGACTAC 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18815R-1.in silico     421 TTCCCAGGCGCCAAGGTGAACAGCGATGATGTGCCGATTTTCCAGGCGCATGGCGACTAC 480


18815R-1.IR_full       481 GATCCCGTGGTGCCGTACAAATT 503
                           |||||||||||||||||||||||
18815R-1.in silico     481 GATCCCGTGGTGCCGTACAAATT 503



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_144417.2  CG18815-RA, transcript variant A (CG18815), mRNA 
100   482  0  0  0  NM_206334.1  CG18815-RB, transcript variant B (CG18815), mRNA 
100   482  0  0  0  NM_206333.1  CG18815-RC, transcript variant C (CG18815), mRNA 
0   0  4  7  3  NM_132125.1  CG4536-RA (CG4536), mRNA 
0   0  3  5  4  NM_164600.2  CG31660-RB, transcript variant B (CG31660), mRNA 
0   0  3  5  4  NM_001014465.1  CG31660-RC, transcript variant C (CG31660), mRNA 
0   0  2  5  2  NM_145106.1  CG6964-RA, transcript variant A (Gug), mRNA 
0   0  2  5  2  NM_079249.2  CG6964-RB, transcript variant B (Gug), mRNA 
0   0  2  5  2  NM_176300.1  CG6964-RC, transcript variant C (Gug), mRNA 
0   0  2  5  2  NM_206303.1  CG6964-RD, transcript variant D (Gug), mRNA 
0   0  2  2  2  NM_135353.2  CG8349-RA (CG8349), mRNA 
0   0  1  17  13  NM_141768.2  CG6567-RA (CG6567), mRNA 
0   0  1  4  6  NM_167150.1  CG2151-RC, transcript variant C (Trxr-1), mRNA 
0   0  1  4  6  NM_167149.1  CG2151-RB, transcript variant B (Trxr-1), mRNA 
0   0  1  4  6  NM_078527.2  CG2151-RA, transcript variant A (Trxr-1), mRNA 
0   0  1  3  5  NM_001014624.1  CG33555-RC, transcript variant C (btsz), mRNA 
0   0  1  3  5  NM_206626.1  CG33220-RA (CG33220), mRNA 
0   0  1  3  4  NM_135004.1  CG3355-RA, transcript variant A (CG3355), mRNA 
0   0  1  3  4  NM_164594.1  CG3355-RB, transcript variant B (CG3355), mRNA 
0   0  1  3  3  NM_001038916.1  CG17689-RB, transcript variant B (CG17689), mRNA 
0   0  1  3  3  NM_140402.2  CG17689-RA, transcript variant A (CG17689), mRNA 
0   0  1  3  2  NM_170370.1  CG9983-RA, transcript variant A (Hrb98DE), mRNA 
0   0  1  3  2  NM_170371.1  CG9983-RE, transcript variant E (Hrb98DE), mRNA 
0   0  1  3  2  NM_079819.2  CG9983-RB, transcript variant B (Hrb98DE), mRNA 
0   0  1  3  2  NM_170374.1  CG9983-RF, transcript variant F (Hrb98DE), mRNA 
0   0  1  3  2  NM_170372.1  CG9983-RC, transcript variant C (Hrb98DE), mRNA 
0   0  1  3  2  NM_170373.1  CG9983-RD, transcript variant D (Hrb98DE), mRNA 
0   0  1  2  2  NM_142564.2  CG7360-RA (Nup58), mRNA 
0   0  1  2  2  NM_168217.2  CG7546-RB, transcript variant B (CG7546), mRNA 
0   0  1  2  2  NM_139875.3  CG7546-RA, transcript variant A (CG7546), mRNA 

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