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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 18747R-1 
 Symbol CG18747  Full Name CG18747 
 CG No CG18747  Old CG No CG18747 
 Synonyms CG18747 
 Accession No (Link to NCBI) NM_144393.1 
 Inserted Chr. ll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgACTGGTAAAAGGATGCGCGAC 
 Primer Seq. 3' 
aatctagaggtaccGTGGAAGGTGCAGCTCCAGTT 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 ACTGGTAAAA GGATGCGCGA CCCTTAAATG TGACAGGTCC AAGGAGGTTC AAGCTGTGCT 
0061 GCTCAAGGTA GTCGGATATT CTATAACCAA GTGGAGCGAA AAGAGTCTGG GCTCGACAAA 
0121 ACTCTACGTG GGCCGGGAGG ATTATCTATC ATCAAATACT TATCTCGTGG GTTCCGAGCA 
0181 AAACAACACT CACAACAACA CTCACAGACT CACTATTGAG GCTGGGGTTC ACAGCTACAA 
0241 CTTCACCTGC CAGCTGCCCT ATCAATGCCC CTCGTCCTTT GAGGGTCGCC ATGGATgCNT 
0301 CCGCTATATA GTGAAAGTCC TGCTGATCAG ACCGTGGAAG TTCGATCAGG CCTACACAAA 
0361 GGGCTTCACG GTTCTCAAGA TGCTGGATCT CAACTTTGAC ACTCCCCAGC TAAAGTCCGC 
0421 TGCCCACAGC GAGGGTTACC GCACCTTTTG CTGTGGTCCA TGCAAGACGG ATCCTTTAAA 
0481 ACTGGAGCTG CACCTTCCAC  
 in silico PCR Fragment 
0001 ACTGGTAAAA GGATGCGCGA CCCTTAAATG TGACAGGTCC AAGGAGGTTC AAGCTGTGCT 
0061 GCTCAAGGTA GTCGGATATT CTATAACCAA GTGGAGCGAA AAGAGTCTGG GCTCGACAAA 
0121 ACTCTACGTG GGCCGGGAGG ATTATCTATC ATCAAATACT TATCTCGTGG GTTCCGAGCA 
0181 AAACAACACT CACAACAACA CTCACAGACT CACTATTGAG GCTGGGGTTC ACAGCTACAA 
0241 CTTCACCTGC CAGCTGCCCT ATCAATGCCC CTCGTCCTTT GAGGGTCGCC ATGGATGCAT 
0301 CCGCTATATA GTGAAAGTCC TGCTGATCAG ACCGTGGAAG TTCGATCAGG CCTACACAAA 
0361 GGGCTTCACG GTTCTCAAGA TGCTGGATCT CAACTTTGAC ACTCCCCAGC TAAAGTCCGC 
0421 TGCCCACAGC GAGGGTTACC GCACCTTTTG CTGTGGTCCA TGCAAGACGG ATCCTTTAAA 
0481 ACTGGAGCTG CACCTTCCAC  
 Assemble Data 

18747R-1.IR_full       1   ACTGGTAAAAGGATGCGCGACCCTTAAATGTGACAGGTCCAAGGAGGTTCAAGCTGTGCT 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18747R-1.in silico     1   ACTGGTAAAAGGATGCGCGACCCTTAAATGTGACAGGTCCAAGGAGGTTCAAGCTGTGCT 60


18747R-1.IR_full       61  GCTCAAGGTAGTCGGATATTCTATAACCAAGTGGAGCGAAAAGAGTCTGGGCTCGACAAA 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18747R-1.in silico     61  GCTCAAGGTAGTCGGATATTCTATAACCAAGTGGAGCGAAAAGAGTCTGGGCTCGACAAA 120


18747R-1.IR_full       121 ACTCTACGTGGGCCGGGAGGATTATCTATCATCAAATACTTATCTCGTGGGTTCCGAGCA 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18747R-1.in silico     121 ACTCTACGTGGGCCGGGAGGATTATCTATCATCAAATACTTATCTCGTGGGTTCCGAGCA 180


18747R-1.IR_full       181 AAACAACACTCACAACAACACTCACAGACTCACTATTGAGGCTGGGGTTCACAGCTACAA 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18747R-1.in silico     181 AAACAACACTCACAACAACACTCACAGACTCACTATTGAGGCTGGGGTTCACAGCTACAA 240


18747R-1.IR_full       241 CTTCACCTGCCAGCTGCCCTATCAATGCCCCTCGTCCTTTGAGGGTCGCCATGGATGCNT 300
                           |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| |
18747R-1.in silico     241 CTTCACCTGCCAGCTGCCCTATCAATGCCCCTCGTCCTTTGAGGGTCGCCATGGATGCAT 300


18747R-1.IR_full       301 CCGCTATATAGTGAAAGTCCTGCTGATCAGACCGTGGAAGTTCGATCAGGCCTACACAAA 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18747R-1.in silico     301 CCGCTATATAGTGAAAGTCCTGCTGATCAGACCGTGGAAGTTCGATCAGGCCTACACAAA 360


18747R-1.IR_full       361 GGGCTTCACGGTTCTCAAGATGCTGGATCTCAACTTTGACACTCCCCAGCTAAAGTCCGC 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18747R-1.in silico     361 GGGCTTCACGGTTCTCAAGATGCTGGATCTCAACTTTGACACTCCCCAGCTAAAGTCCGC 420


18747R-1.IR_full       421 TGCCCACAGCGAGGGTTACCGCACCTTTTGCTGTGGTCCATGCAAGACGGATCCTTTAAA 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18747R-1.in silico     421 TGCCCACAGCGAGGGTTACCGCACCTTTTGCTGTGGTCCATGCAAGACGGATCCTTTAAA 480


18747R-1.IR_full       481 ACTGGAGCTGCACCTTCCAC 500
                           ||||||||||||||||||||
18747R-1.in silico     481 ACTGGAGCTGCACCTTCCAC 500



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_144393.1  CG18747-RA (CG18747), mRNA 
0.2   1  2  3  2  NM_166429.1  CG9433-RA, transcript variant A (Xpd), mRNA 
0.2   1  2  3  2  NM_057830.3  CG9433-RB, transcript variant B (Xpd), mRNA 
0   0  3  9  9  NM_141479.2  CG10086-RA (CG10086), mRNA 
0   0  3  2  3  NM_142913.1  CG10254-RA, transcript variant A (CG10254), mRNA 
0   0  3  2  3  NM_170081.1  CG10254-RB, transcript variant B (CG10254), mRNA 
0   0  1  5  18  NM_001014630.1  CG33547-RA (Rim), mRNA 
0   0  1  4  6  NM_078740.2  CG8817-RB, transcript variant B (lilli), mRNA 
0   0  1  4  6  NM_164516.1  CG8817-RA, transcript variant A (lilli), mRNA 
0   0  1  4  5  NM_141431.1  CG1105-RA (CG1105), mRNA 
0   0  1  3  5  NM_132230.2  CG2272-RA (slpr), mRNA 
0   0  1  3  3  NM_167354.1  CG1771-RA, transcript variant A (mew), mRNA 
0   0  1  3  3  NM_079853.2  CG15532-RA, transcript variant A (hdc), mRNA 
0   0  1  3  3  NM_176591.1  CG15532-RC, transcript variant C (hdc), mRNA 
0   0  1  3  3  NM_170478.1  CG15532-RB, transcript variant B (hdc), mRNA 
0   0  1  3  3  NM_078590.2  CG1771-RB, transcript variant B (mew), mRNA 
0   0  1  3  3  NM_079282.1  CG3322-RA (LanB2), mRNA 
0   0  1  3  2  NM_078826.3  CG6555-RA, transcript variant A (CG6555), mRNA 
0   0  1  3  2  NM_001032083.1  CG15841-RA, transcript variant A (CG15841), mRNA 
0   0  1  3  2  NM_130676.1  CG14417-RA (CG14417), mRNA 
0   0  1  3  2  NM_166878.1  CG11412-RA, transcript variant A (CG11412), mRNA 
0   0  1  3  2  NM_130547.3  CG11412-RC, transcript variant C (CG11412), mRNA 
0   0  1  3  2  NM_166879.1  CG11412-RB, transcript variant B (CG11412), mRNA 
0   0  1  2  6  NM_143765.2  CG12218-RA (mei-P26), mRNA 
0   0  1  2  6  NM_079198.2  CG15010-RC, transcript variant C (ago), mRNA 
0   0  1  2  6  NM_168073.1  CG15010-RB, transcript variant B (ago), mRNA 
0   0  1  2  6  NM_168072.1  CG15010-RA, transcript variant A (ago), mRNA 
0   0  1  2  2  NM_135056.3  CG31919-RC, transcript variant C (CG31919), mRNA 
0   0  1  2  2  NM_137517.4  CG18604-RA (CG18604), mRNA 
0   0  1  2  2  NM_164622.2  CG31919-RB, transcript variant B (CG31919), mRNA 

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