NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 18662R-3

Symbol CG18662 Full Name CG18662

CG No CG18662 Old CG No CG18662

Synonyms CG18662

Accession No (Link to NCBI) NM_144304.1

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees viable

Map Viewer

Reference
[Please submit your publication] Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCCGTTTGGCGGCCAATGATGC

Primer Seq. 3'

aatctagaggtaccGGGCTCCATTCCACCAGATTC

Predicted Fragment Size

IR fragment full Seq

0001 CCGCNTTTGG CGGCCAATGA TGCAAGTGGC TCGACTTTAT TGTGAGAANG CCGATGCGAA 
0061 GTCTAGTCGC TTTTCCTGTT GCCAAGGTGG AAAGTGGAAA ATCGAAATAC ATGATGGCCC 
0121 ACGTTTACAT CCATGGGGAA ATGGGTAGCG CCAAGCAGGT GATTCGTAGC CTCTCCAAGG 
0181 CCAAGTACCA TCTTGATGTA TACGATGAGT TAAAAAAGGA AGCTGAGTCG ATGGGCTTGT 
0241 GCACCCAGGG TCTTGGAGGT GGTTACTTGG TTCACGACAA GGAAAAGAAG TACATCAAGC 
0301 TGTATGGAAG ATCACAGGCC CTGGGAAAAG CTGATCACGA AGCTGCCCGT GAGATATTGC 
0361 AACCCATCTA TACCGATCAC AAGATCGATG CTGAATCTGG TGGAATGNGA GCCC

in silico PCR Fragment

0001 CCG--TTTGG CGGCCAATGA TGCAAGCGGC TCGACTTTAT TGTGAGAA-G CCAATGCGAA 
0061 GTCTAGTCGC TTTTCCTGTT GCCAAGGTGG AAAGTGGAAA ATCAAAGTAC ATGATGGCCC 
0121 ACGTTTATAT CCATGGGGAA ATGGGTAGCG CCAAGCAGGT GATTCGTAGC CTTTCCAAGG 
0181 CCAAGTACCA TCTTGATGTA TACGATGAGT TGAAAAAGGA GGCTGAGTCG ATGGGCTTGT 
0241 GCACCCAGGG TCTTGGAGGT GGTTACTTGG TTCACGACAA GGAAAAGAAG TACATCAAGC 
0301 TGTATGGAAG ATCACAGGCC CTGGGAAAAG CTGATCACGA AGCTGCCCGT GAGATATTGC 
0361 AACCCATCTA TACCGATCAC AAGATCGATG CCGAATCTGG TGGAATG-GA GCCC

Assemble Data


18662R-3.IR_full       1   CCGCNTTTGGCGGCCAATGATGCAAGTGGCTCGACTTTATTGTGAGAANGCCGATGCGAA 60
                           |||  ||||||||||||||||||||| ||||||||||||||||||||| ||| |||||||
18662R-3.in silico     1   CCG--TTTGGCGGCCAATGATGCAAGCGGCTCGACTTTATTGTGAGAA-GCCAATGCGAA 60


18662R-3.IR_full       61  GTCTAGTCGCTTTTCCTGTTGCCAAGGTGGAAAGTGGAAAATCGAAATACATGATGGCCC 120
                           ||||||||||||||||||||||||||||||||||||||||||| || |||||||||||||
18662R-3.in silico     61  GTCTAGTCGCTTTTCCTGTTGCCAAGGTGGAAAGTGGAAAATCAAAGTACATGATGGCCC 120


18662R-3.IR_full       121 ACGTTTACATCCATGGGGAAATGGGTAGCGCCAAGCAGGTGATTCGTAGCCTCTCCAAGG 180
                           ||||||| |||||||||||||||||||||||||||||||||||||||||||| |||||||
18662R-3.in silico     121 ACGTTTATATCCATGGGGAAATGGGTAGCGCCAAGCAGGTGATTCGTAGCCTTTCCAAGG 180


18662R-3.IR_full       181 CCAAGTACCATCTTGATGTATACGATGAGTTAAAAAAGGAAGCTGAGTCGATGGGCTTGT 240
                           ||||||||||||||||||||||||||||||| |||||||| |||||||||||||||||||
18662R-3.in silico     181 CCAAGTACCATCTTGATGTATACGATGAGTTGAAAAAGGAGGCTGAGTCGATGGGCTTGT 240


18662R-3.IR_full       241 GCACCCAGGGTCTTGGAGGTGGTTACTTGGTTCACGACAAGGAAAAGAAGTACATCAAGC 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18662R-3.in silico     241 GCACCCAGGGTCTTGGAGGTGGTTACTTGGTTCACGACAAGGAAAAGAAGTACATCAAGC 300


18662R-3.IR_full       301 TGTATGGAAGATCACAGGCCCTGGGAAAAGCTGATCACGAAGCTGCCCGTGAGATATTGC 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18662R-3.in silico     301 TGTATGGAAGATCACAGGCCCTGGGAAAAGCTGATCACGAAGCTGCCCGTGAGATATTGC 360


18662R-3.IR_full       361 AACCCATCTATACCGATCACAAGATCGATGCTGAATCTGGTGGAATGNGAGCCC 414
                           ||||||||||||||||||||||||||||||| ||||||||||||||| ||||||
18662R-3.in silico     361 AACCCATCTATACCGATCACAAGATCGATGCCGAATCTGGTGGAATG-GAGCCC 414

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	392	0	0	0	NM_144304.1	CG18662-RA (CG18662), mRNA
0	0	2	3	2	NM_206476.1	CG31368-RB, transcript variant B (CG31368), mRNA
0	0	2	3	2	NM_169439.2	CG31368-RA, transcript variant A (CG31368), mRNA
0	0	2	2	4	NM_001043095.1	CG15078-RB, transcript variant B (Mctp), mRNA
0	0	2	2	4	NM_137528.3	CG15078-RA, transcript variant A (Mctp), mRNA
0	0	2	2	4	NM_001043094.1	CG15078-RC, transcript variant C (Mctp), mRNA
0	0	2	2	3	NM_132260.2	CG12113-RA (l(1)G0095), mRNA
0	0	1	7	5	NM_169923.1	CG31195-RA (CG31195), mRNA
0	0	1	3	2	NM_142703.2	CG5911-RA, transcript variant A (ETHR), mRNA
0	0	1	2	4	NM_132230.2	CG2272-RA (slpr), mRNA
0	0	1	2	4	NM_140344.1	CG10967-RA (Atg1), mRNA
0	0	1	2	3	NM_057915.3	CG9749-RA (Abi), mRNA
0	0	1	2	2	NM_137867.1	CG13519-RA (Obp58d), mRNA
0	0	0	6	4	NM_166677.1	CG3541-RB, transcript variant B (pio), mRNA
0	0	0	6	4	NM_079684.3	CG4510-RA (Surf6), mRNA
0	0	0	6	3	NM_138094.2	CG3541-RC, transcript variant C (pio), mRNA
0	0	0	5	3	NM_140510.2	CG7275-RA (CG7275), mRNA
0	0	0	4	6	NM_142331.1	CG4009-RA (CG4009), mRNA
0	0	0	3	7	NM_141360.1	CG1077-RA (CG1077), mRNA
0	0	0	3	7	NM_176585.1	CG7933-RB, transcript variant B (janA), mRNA
0	0	0	3	7	NM_176586.2	CG7933-RA, transcript variant A (janA), mRNA
0	0	0	3	7	NM_206588.1	CG7933-RC, transcript variant C (janA), mRNA
0	0	0	3	6	NM_139946.1	CG7194-RA (CG7194), mRNA
0	0	0	3	4	NM_137806.2	CG5465-RA (MED16), mRNA
0	0	0	3	4	NM_132388.2	CG2909-RA (CG2909), mRNA
0	0	0	3	4	NM_136623.1	CG8014-RA (Rme-8), mRNA
0	0	0	3	4	NM_142463.2	CG7678-RA (CG7678), mRNA
0	0	0	3	2	NM_135261.1	CG4567-RA (CG4567), mRNA
0	0	0	2	9	NM_078867.2	CG5526-RA (Dhc36C), mRNA
0	0	0	2	8	NM_079504.3	CG1133-RA (opa), mRNA

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