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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
18657R-2 |
| Symbol |
NetA |
Full Name |
Netrin-A |
| CG No |
CG18657 |
Old CG No |
CG18657 |
| Synonyms |
netA, CT27014, CG18657, NetA |
| Accession No (Link to NCBI) |
NM_078599.3 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Graca FA, Rai M, Hunt LC, Stephan A, Wang YD, Gordon B, Wang R, Quarato G, Xu B, Fan Y, Labelle M, Demontis F.
The myokine Fibcd1 is an endogenous determinant of myofiber size and mitigates cancer-induced myofiber atrophy.
Nat Commun (2022) 13(1) 2370
[
PubMed ID = 35501350
]
[
RRC reference
]
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Perkins AD, Lee MJ, Tanentzapf G.
The systematic identification of cytoskeletal genes required for Drosophila melanogaster muscle maintenance.
Sci Data (2014) 1 140002
[
PubMed ID = 25977760
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgCGTGGAATCTTGCTCCTGCTG |
| Primer Seq. 3' |
aatctagaggtaccCAACTGAGCCCGTGATCGGTG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 CGTGGAATCT TGCTCCTGCT GCTGGGAACC ACCAGGTTCA GCCCCATCCA GTGCATCTTC
0061 AATGACGTGT ACTTCAAGAT GTTTTCGCAA CAGGCGCCGC CGGAGGATCC GTGCTACAAT
0121 AAAGCGCACG AGCCACGCGC CTGCATTCCG GACTTTGTGA ACGCCGCCTA CGATGCTCCA
0181 GTGGTGGCCA GTTCCACGTG CGGATCGTCG GGTGCGCAGC GATATTGCGA GTACCAGGAT
0241 CACGAGCGCT CCTGCCACAC CTGCGACATG ACGGATCCGT TGCGCAGCTT TCCAGCCCGC
0301 TCGCTGACCG ATTTGAATAA CTCGAATAAT GTGACCTGCT GGCGCAGTGA GCCGGTGACC
0361 GGAAGTGGGG ACAACGTAAC CTTGACCCTC TCGCTGGGCA AGAAGTTCGA GCTGACCTAT
0421 GTGATCCTAC AGCTGTGCCC GCATGCTCGC GCGTCCCGAT TCGATGGTGA TCTACAAGAG
0481 CACCGATCAC GGGCTCAGTT G |
| in silico PCR Fragment |
0001 CGTGGAATCT TGCTCCTGCT GCTGGGAACC ACCAGGTTCA GCCCCATCCA GTGCATCTCC
0061 AATGACGTGT ACTTCAAGAT GTTTTCGCAA CAGGCGCCGC CGGAGGATCC GTGCTACAAT
0121 AAAGCGCACG AGCCACGCGC CTGCATTCCG GACTTTGTGA ACGCCGCCTA CGATGCTCCA
0181 GTGGTGGCCA GTTCCACGTG CGGATCGTCG GGTGCGCAGC GATATTGCGA GTACCAGGAT
0241 CACGAGCGCT CCTGCCACAC CTGCGACATG ACGGATCCGT TGCGCAGCTT TCCAGCCCGC
0301 TCGCTGACCG ATTTGAATAA CTCGAATAAT GTGACCTGCT GGCGCAGTGA GCCGGTGACC
0361 GGAAGTGGGG ACAACGTAAC CTTGACCCTC TCGCTGGGCA AGAAGTTCGA GCTGACCTAT
0421 GTGATCCTAC AGCTGTGCCC GCATGCTC-C GCGTCCCGAT TCGATGGTGA TCTACAAGAG
0481 CACCGATCAC GGGCTCAGTT G |
| Assemble Data |
18657R-2.IR_full 1 CGTGGAATCTTGCTCCTGCTGCTGGGAACCACCAGGTTCAGCCCCATCCAGTGCATCTTC 60
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||| |
18657R-2.in silico 1 CGTGGAATCTTGCTCCTGCTGCTGGGAACCACCAGGTTCAGCCCCATCCAGTGCATCTCC 60
18657R-2.IR_full 61 AATGACGTGTACTTCAAGATGTTTTCGCAACAGGCGCCGCCGGAGGATCCGTGCTACAAT 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18657R-2.in silico 61 AATGACGTGTACTTCAAGATGTTTTCGCAACAGGCGCCGCCGGAGGATCCGTGCTACAAT 120
18657R-2.IR_full 121 AAAGCGCACGAGCCACGCGCCTGCATTCCGGACTTTGTGAACGCCGCCTACGATGCTCCA 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18657R-2.in silico 121 AAAGCGCACGAGCCACGCGCCTGCATTCCGGACTTTGTGAACGCCGCCTACGATGCTCCA 180
18657R-2.IR_full 181 GTGGTGGCCAGTTCCACGTGCGGATCGTCGGGTGCGCAGCGATATTGCGAGTACCAGGAT 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18657R-2.in silico 181 GTGGTGGCCAGTTCCACGTGCGGATCGTCGGGTGCGCAGCGATATTGCGAGTACCAGGAT 240
18657R-2.IR_full 241 CACGAGCGCTCCTGCCACACCTGCGACATGACGGATCCGTTGCGCAGCTTTCCAGCCCGC 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18657R-2.in silico 241 CACGAGCGCTCCTGCCACACCTGCGACATGACGGATCCGTTGCGCAGCTTTCCAGCCCGC 300
18657R-2.IR_full 301 TCGCTGACCGATTTGAATAACTCGAATAATGTGACCTGCTGGCGCAGTGAGCCGGTGACC 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18657R-2.in silico 301 TCGCTGACCGATTTGAATAACTCGAATAATGTGACCTGCTGGCGCAGTGAGCCGGTGACC 360
18657R-2.IR_full 361 GGAAGTGGGGACAACGTAACCTTGACCCTCTCGCTGGGCAAGAAGTTCGAGCTGACCTAT 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18657R-2.in silico 361 GGAAGTGGGGACAACGTAACCTTGACCCTCTCGCTGGGCAAGAAGTTCGAGCTGACCTAT 420
18657R-2.IR_full 421 GTGATCCTACAGCTGTGCCCGCATGCTCGCGCGTCCCGATTCGATGGTGATCTACAAGAG 480
|||||||||||||||||||||||||||| |||||||||||||||||||||||||||||||
18657R-2.in silico 421 GTGATCCTACAGCTGTGCCCGCATGCTC-CGCGTCCCGATTCGATGGTGATCTACAAGAG 480
18657R-2.IR_full 481 CACCGATCACGGGCTCAGTTG 501
|||||||||||||||||||||
18657R-2.in silico 481 CACCGATCACGGGCTCAGTTG 501
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_078599.3 |
CG18657-RA (NetA), mRNA |
| 0.41 |
2 |
4 |
2 |
2 |
NM_137130.2 |
CG10131-RA (CG10131), mRNA |
| 0 |
0 |
16 |
17 |
12 |
NM_078600.2 |
CG10521-RA (NetB), mRNA |
| 0 |
0 |
7 |
2 |
3 |
NM_001015336.1 |
CG40380-PA.3 (CG40380), mRNA |
| 0 |
0 |
2 |
5 |
2 |
NM_136446.2 |
CG1603-RA (CG1603), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_165041.1 |
CG6866-RA, transcript variant A (loqs), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_135802.2 |
CG6866-RB, transcript variant B (loqs), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_001038814.1 |
CG6866-RC, transcript variant C (loqs), mRNA |
| 0 |
0 |
1 |
7 |
3 |
NM_001043132.1 |
CG3689-RC, transcript variant C (CG3689), mRNA |
| 0 |
0 |
1 |
7 |
3 |
NM_140051.2 |
CG3689-RB, transcript variant B (CG3689), mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_170089.1 |
CG10367-RA, transcript variant A (Hmgcr), mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_136507.1 |
CG11210-RA (CG11210), mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_206548.1 |
CG10367-RB, transcript variant B (Hmgcr), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NR_002490.1 |
CG10367-RB, transcript variant B (Hmgcr), mRNA, miscRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_168799.1 |
CG32212-RA (CG32212), mRNA |
| 0 |
0 |
1 |
3 |
11 |
NM_078604.3 |
CG32592-RA (hiw), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_130594.1 |
CG14803-RA (CG14803), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_143225.2 |
CG6490-RA (CG6490), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_170552.1 |
CG1499-RB, transcript variant B (CG1499), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_143614.1 |
CG1499-RA, transcript variant A (CG1499), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_132629.1 |
CG4330-RA (CG4330), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_144289.1 |
CG15308-RA (CG15308), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_142213.1 |
CG14870-RA (CG14870), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_164771.2 |
CG7179-RA (CG7179), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_132335.2 |
CG12139-RB (CG12139), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_057757.3 |
CG2714-RA, transcript variant A (crm), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_079528.2 |
CG2520-RA (lap), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_166951.1 |
CG2714-RB, transcript variant B (crm), mRNA |
| 0 |
0 |
0 |
7 |
8 |
NM_176104.1 |
CG33087-RC (CG33087), mRNA |
| 0 |
0 |
0 |
6 |
6 |
NM_135841.4 |
CG8954-RA, transcript variant A (Smg5), mRNA |
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