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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
18548R-1 |
| Symbol |
GstD10 |
Full Name |
Glutathione S transferase D10 |
| CG No |
CG18548 |
Old CG No |
CG18548 |
| Synonyms |
DmGSTD10, CG17392, CG18548, GstD10, Glutathione S transferase D10 |
| Accession No (Link to NCBI) |
NM_144456.1 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
pupal semi-lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
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| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgTATAGACCCGGATCTGCTCCC |
| Primer Seq. 3' |
aatctagaggtaccCCAGCCACATCGAAAGTGGAA |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 TATAGACCCG GATCTGCTCC CTGCCGCTCT GTTCTGATGA CAGCCAAGGC ACTGGGTGTG
0061 GAGTTCGATA AGAAGACCAT TATCAACACC CGAGCTAGGG AGCAATTCAC GCCGGAATAC
0121 CTGAAAATCA ATCCGCAGCA CACGATCCCC ACGCTGCACG ACCATGGATT TGCTCTGTGG
0181 GAGTCGCGGG CGATTATGGT TTATCTGGTG GAGAAGTACG GCAAGGACGA CAAGCTCTTC
0241 CCCAAGGATG TGCAAAAGCA GGCGTTGATC AATCAGCGCC TGTACTTCGA CATGGGTACG
0301 CTGTATAAGA GCTTCTCCGA GTACTATTAT CCGCAGATTT TCCTAAAGAA GCCCGCCAAT
0361 GAGGAGAACT ACAAGAAGAT CGAAGTGGCC TTCGAATTCC TAAACACATT CCTGGAGGGG
0421 CAGACCTACA GCGCTGGAGG GGACTATAGC TTGGCGGATA TTGCCTTTCT GGCCACCGTT
0481 TCCACTTTCG ATGTGGCTGG |
| in silico PCR Fragment |
0001 TATAGACCCG GATCTGCTCC CTGCCGCTCT GTTCTGATGA CAGCCAAGGC ACTGGGTGTG
0061 GAGTTCGATA AGAAGACCAT TATCAACACC CGAGCTAGGG AGCAATTCAC GCCGGAATAC
0121 CTGAAAATCA ATCCGCAGCA CACGATCCCC ACGCTGCACG ACCATGGATT TGCTTTGTGG
0181 GAGTCGCGGG CGATTATGGT TTATCTGGTG GAGAAGTACG GCAAGGACGA CAAGCTCTTC
0241 CCCAAGGATG TGCAAAAGCA GGCGTTGATC AATCAGCGCC TGTACTTCGA CATGGGTACG
0301 CTGTATAAGA GCTTCTCCGA GTACTATTAT CCGCAGATTT TCCTAAAGAA GCCCGCCAAT
0361 GAGGAGAACT ACAAGAAGAT CGAAGTGGCC TTCGAATTCC TAAACACATT CCTGGAGGGG
0421 CAGACCTACA GCGCTGGAGG GGATTATAGC TTGGCGGATA TTGCCTTTCT GGCCACCGTT
0481 TCCACTTTCG ATGTGGCTGG |
| Assemble Data |
18548R-1.IR_full 1 TATAGACCCGGATCTGCTCCCTGCCGCTCTGTTCTGATGACAGCCAAGGCACTGGGTGTG 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18548R-1.in silico 1 TATAGACCCGGATCTGCTCCCTGCCGCTCTGTTCTGATGACAGCCAAGGCACTGGGTGTG 60
18548R-1.IR_full 61 GAGTTCGATAAGAAGACCATTATCAACACCCGAGCTAGGGAGCAATTCACGCCGGAATAC 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18548R-1.in silico 61 GAGTTCGATAAGAAGACCATTATCAACACCCGAGCTAGGGAGCAATTCACGCCGGAATAC 120
18548R-1.IR_full 121 CTGAAAATCAATCCGCAGCACACGATCCCCACGCTGCACGACCATGGATTTGCTCTGTGG 180
|||||||||||||||||||||||||||||||||||||||||||||||||||||| |||||
18548R-1.in silico 121 CTGAAAATCAATCCGCAGCACACGATCCCCACGCTGCACGACCATGGATTTGCTTTGTGG 180
18548R-1.IR_full 181 GAGTCGCGGGCGATTATGGTTTATCTGGTGGAGAAGTACGGCAAGGACGACAAGCTCTTC 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18548R-1.in silico 181 GAGTCGCGGGCGATTATGGTTTATCTGGTGGAGAAGTACGGCAAGGACGACAAGCTCTTC 240
18548R-1.IR_full 241 CCCAAGGATGTGCAAAAGCAGGCGTTGATCAATCAGCGCCTGTACTTCGACATGGGTACG 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18548R-1.in silico 241 CCCAAGGATGTGCAAAAGCAGGCGTTGATCAATCAGCGCCTGTACTTCGACATGGGTACG 300
18548R-1.IR_full 301 CTGTATAAGAGCTTCTCCGAGTACTATTATCCGCAGATTTTCCTAAAGAAGCCCGCCAAT 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18548R-1.in silico 301 CTGTATAAGAGCTTCTCCGAGTACTATTATCCGCAGATTTTCCTAAAGAAGCCCGCCAAT 360
18548R-1.IR_full 361 GAGGAGAACTACAAGAAGATCGAAGTGGCCTTCGAATTCCTAAACACATTCCTGGAGGGG 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18548R-1.in silico 361 GAGGAGAACTACAAGAAGATCGAAGTGGCCTTCGAATTCCTAAACACATTCCTGGAGGGG 420
18548R-1.IR_full 421 CAGACCTACAGCGCTGGAGGGGACTATAGCTTGGCGGATATTGCCTTTCTGGCCACCGTT 480
||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||||
18548R-1.in silico 421 CAGACCTACAGCGCTGGAGGGGATTATAGCTTGGCGGATATTGCCTTTCTGGCCACCGTT 480
18548R-1.IR_full 481 TCCACTTTCGATGTGGCTGG 500
||||||||||||||||||||
18548R-1.in silico 481 TCCACTTTCGATGTGGCTGG 500
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_144456.1 |
CG18548-RA (GstD10), mRNA |
| 3.11 |
15 |
15 |
34 |
68 |
NM_079602.3 |
CG10045-RA, transcript variant A (GstD1), mRNA |
| 3.11 |
15 |
15 |
34 |
68 |
NM_001038953.1 |
CG10045-RB, transcript variant B (GstD1), mRNA |
| 1.86 |
9 |
12 |
26 |
34 |
NM_080175.2 |
CG12242-RA (GstD5), mRNA |
| 1.65 |
8 |
14 |
24 |
25 |
NM_080173.1 |
CG4181-RA (GstD2), mRNA |
| 1.03 |
5 |
13 |
45 |
56 |
NM_176479.1 |
CG4381-RA (GstD3), mRNA |
| 1.03 |
5 |
4 |
15 |
38 |
NM_080375.1 |
CG4371-RA (GstD7), mRNA |
| 0.82 |
4 |
6 |
23 |
28 |
NM_141924.1 |
CG10091-RA (GstD9), mRNA |
| 0 |
0 |
16 |
24 |
47 |
NM_080174.2 |
CG11512-RA (GstD4), mRNA |
| 0 |
0 |
3 |
7 |
3 |
NM_137495.2 |
CG5224-RA (CG5224), mRNA |
| 0 |
0 |
3 |
7 |
3 |
NM_141795.2 |
CG6693-RA (CG6693), mRNA |
| 0 |
0 |
2 |
5 |
4 |
NM_079175.2 |
CG1242-RA (Hsp83), mRNA |
| 0 |
0 |
2 |
4 |
5 |
NM_078829.1 |
CG16960-RA (Or33a), mRNA |
| 0 |
0 |
2 |
3 |
8 |
NM_167989.1 |
CG11537-RB, transcript variant B (CG11537), mRNA |
| 0 |
0 |
2 |
3 |
8 |
NM_167988.1 |
CG11537-RA, transcript variant A (CG11537), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_134725.1 |
CG4552-RA (CG4552), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_136386.2 |
CG3409-RA (CG3409), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_079615.2 |
CG7756-RA (Hsc70-2), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_206474.1 |
CG14723-RC, transcript variant C (HisCl1), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_169429.3 |
CG14723-RA, transcript variant A (HisCl1), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_141859.3 |
CG14723-RB, transcript variant B (HisCl1), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_001042817.1 |
CG4937-RB, transcript variant B (RhoGAP15B), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_132955.1 |
CG4937-RA, transcript variant A (RhoGAP15B), mRNA |
| 0 |
0 |
1 |
2 |
13 |
NM_137482.1 |
CG17525-RA (GstE4), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_143483.1 |
CG7808-RC (RpS8), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_136542.1 |
CG30361-RA, transcript variant A (mXr), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_165609.1 |
CG30361-RB, transcript variant B (mXr), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_136127.1 |
CG13084-RA (CG13084), mRNA |
| 0 |
0 |
0 |
20 |
55 |
NM_080177.1 |
CG4421-RA (GstD8), mRNA |
| 0 |
0 |
0 |
17 |
46 |
NM_080176.1 |
CG4423-RA (GstD6), mRNA |
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| Wing Disc |
GFP
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Puc
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Caspase
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DAPI
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Merge
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Figure
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JNK signal
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Caspase-3 signal
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