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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
discarded
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
18478R-2 |
| Symbol |
CG18478 |
Full Name |
CG18478 |
| CG No |
CG18478 |
Old CG No |
CG18478 |
| Synonyms |
SPH157, DS07108.5, BG:DS07108.5, CG18478 |
| Accession No (Link to NCBI) |
NM_135920.1 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
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Reference
[Please submit your publication]
|
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
Kambris Z, Brun S, Jang IH, Nam HJ, Romeo Y, Takahashi K, Lee WJ, Ueda R, Lemaitre B.
Drosophila immunity: a large-scale in vivo RNAi screen identifies five serine proteases required for Toll activation.
Curr Biol (2006) 16(8) 808-13
[
PubMed ID = 16631589
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgATCCAGATGCTGTGAAAGTGC |
| Primer Seq. 3' |
aatctagaggtaccATCCTGACAGATATGCCTGGG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 NTCCAGATGC TGTGAAAGTG CAATTTAATG TTACCGAAGG GCAAGCAAAA CCAGCCGAGT
0061 TTCCGTGGAC CATTGCCGTG ATCCACAATC GCAGCTTAGT GGGTGGTGGC TCTTTAATTA
0121 CCCCAGACAT TGTGCTCACA GCTGCCCATC GGATATTCAA TAAAGACGTG GAGGATATAG
0181 TCGTCAGCGC AGGAGAATGG GAGTACGGAT CTGCTTTGGA AAAATATCCA TTTGAAGAGG
0241 CATTTGTGTT GAAAATGGTT ATACATAAAT CATTTAATTA TCAGAGAGGC GCCAACAACT
0301 TAGCGCTGCT CTTCCTGGAT AGGGAGTTCC CACTAACATA CAAGATTAAC ACCATCTGTT
0361 TGCCCACCCA GAAAAGATCG CTATCTTCCA CTCGCTGTAT AGTGGCTGGT TGGGGTAAAT
0421 ATCAGTTTAG TGACACGCAT TATGGAGGCG TCCTGAAGAA AATTGATTTA CCTATTGTAC
0481 CCAGGCATAT CTGTCAGGAT |
| in silico PCR Fragment |
0001 ATCCAGATGC TGTGAAAGTG CAATTTAATG TTACCGAAGG GCAAGCAAAA CCAGCCGAGT
0061 TTCCGTGGAC CATTGCCGTG ATCCACAATC GCAGCTTAGT GGGTGGTGGC TCTTTAATTA
0121 CCCCAGACAT TGTGCTCACA GCTGCCCATC GGATATTCAA TAAAGACGTG GAGGATATAG
0181 TCGTCAGCGC AGGAGAATGG GAGTACGGAT CTGCTTTGGA AAAATATCCA TTTGAAGAGG
0241 CATTTGTGTT GAAAATGGTT ATACATAAAT CATTTAATTA TCAGAGAGGC GCCAACAACT
0301 TAGCGCTGCT CTTCCTGGAT AGGGAGTTCC CACTAACATA CAAGATTAAC ACCATTTGTT
0361 TGCCCACCCA GAAAAGATCG CTATCTTCCA CTCGCTGTAT AGTGGCTGGT TGGGGTAAAT
0421 ATCAGTTTAG TGACACGCAT TACGGAGGCG TCCTGAAGAA AATTGATTTA CCTATTGTAC
0481 CCAGGCATAT CTGTCAGGAT |
| Assemble Data |
18478R-2.IR_full 1 TCCAGATGCTGTGAAAGTGCAATTTAATGTTACCGAAGGGCAAGCAAAACCAGCCGAGT 59
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18478R-2.in silico 1 TCCAGATGCTGTGAAAGTGCAATTTAATGTTACCGAAGGGCAAGCAAAACCAGCCGAGT 59
18478R-2.IR_full 61 TTCCGTGGACCATTGCCGTGATCCACAATCGCAGCTTAGTGGGTGGTGGCTCTTTAATTA 119
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18478R-2.in silico 61 TTCCGTGGACCATTGCCGTGATCCACAATCGCAGCTTAGTGGGTGGTGGCTCTTTAATTA 119
18478R-2.IR_full 121 CCCCAGACATTGTGCTCACAGCTGCCCATCGGATATTCAATAAAGACGTGGAGGATATAG 179
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18478R-2.in silico 121 CCCCAGACATTGTGCTCACAGCTGCCCATCGGATATTCAATAAAGACGTGGAGGATATAG 179
18478R-2.IR_full 181 TCGTCAGCGCAGGAGAATGGGAGTACGGATCTGCTTTGGAAAAATATCCATTTGAAGAGG 239
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18478R-2.in silico 181 TCGTCAGCGCAGGAGAATGGGAGTACGGATCTGCTTTGGAAAAATATCCATTTGAAGAGG 239
18478R-2.IR_full 241 CATTTGTGTTGAAAATGGTTATACATAAATCATTTAATTATCAGAGAGGCGCCAACAACT 299
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18478R-2.in silico 241 CATTTGTGTTGAAAATGGTTATACATAAATCATTTAATTATCAGAGAGGCGCCAACAACT 299
18478R-2.IR_full 301 TAGCGCTGCTCTTCCTGGATAGGGAGTTCCCACTAACATACAAGATTAACACCATCTGTT 359
||||||||||||||||||||||||||||||||||||||||||||||||||||||| ||||
18478R-2.in silico 301 TAGCGCTGCTCTTCCTGGATAGGGAGTTCCCACTAACATACAAGATTAACACCATTTGTT 359
18478R-2.IR_full 361 TGCCCACCCAGAAAAGATCGCTATCTTCCACTCGCTGTATAGTGGCTGGTTGGGGTAAAT 419
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18478R-2.in silico 361 TGCCCACCCAGAAAAGATCGCTATCTTCCACTCGCTGTATAGTGGCTGGTTGGGGTAAAT 419
18478R-2.IR_full 421 ATCAGTTTAGTGACACGCATTATGGAGGCGTCCTGAAGAAAATTGATTTACCTATTGTAC 479
|||||||||||||||||||||| |||||||||||||||||||||||||||||||||||||
18478R-2.in silico 421 ATCAGTTTAGTGACACGCATTACGGAGGCGTCCTGAAGAAAATTGATTTACCTATTGTAC 479
18478R-2.IR_full 481 CCAGGCATATCTGTCAGGAT 499
||||||||||||||||||||
18478R-2.in silico 481 CCAGGCATATCTGTCAGGAT 499
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_165122.1 |
CG31827-RA (CG31827), mRNA |
| 100 |
482 |
0 |
0 |
0 |
NM_135920.1 |
CG18478-RA (CG18478), mRNA |
| 0 |
0 |
1 |
4 |
5 |
NM_165208.1 |
CG31750-RA (Gr36d), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_206198.1 |
CG9294-RB, transcript variant B (CG9294), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_137772.1 |
CG9294-RA, transcript variant A (CG9294), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_057276.3 |
CG4178-RA (Lsp1beta), mRNA |
| 0 |
0 |
0 |
6 |
8 |
NM_078794.2 |
CG9564-RA (Try29F), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_141998.1 |
CG11670-RA (CG11670), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_142634.2 |
CG4241-RA, transcript variant A (att-ORFA), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_205945.1 |
CG3811-RD, transcript variant D (Oatp30B), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_135449.2 |
CG3811-RB, transcript variant B (Oatp30B), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_205946.1 |
CG3811-RC, transcript variant C (Oatp30B), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_164857.1 |
CG3811-RA, transcript variant A (Oatp30B), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_143379.2 |
CG1647-RA (CG1647), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_078699.2 |
CG1692-RA (mal), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_001038864.1 |
CG4927-RC (CG4927), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_176342.1 |
CG11661-RH, transcript variant H (Nc73EF), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_168704.2 |
CG11661-RE, transcript variant E (Nc73EF), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_168701.2 |
CG11661-RA, transcript variant A (Nc73EF), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_168702.1 |
CG11661-RB, transcript variant B (Nc73EF), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_176341.1 |
CG11661-RG, transcript variant G (Nc73EF), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_176340.1 |
CG11661-RF, transcript variant F (Nc73EF), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_168703.2 |
CG11661-RC, transcript variant C (Nc73EF), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_141242.1 |
CG1126-RA (CG1126), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_079603.2 |
CG5657-RA (Scgbeta), mRNA |
| 0 |
0 |
0 |
3 |
11 |
NM_168346.1 |
CG32043-RB, transcript variant B (CG32043), mRNA |
| 0 |
0 |
0 |
3 |
11 |
NM_140064.2 |
CG32043-RA, transcript variant A (CG32043), mRNA |
| 0 |
0 |
0 |
3 |
8 |
NM_132932.2 |
CG9634-RA (CG9634), mRNA |
| 0 |
0 |
0 |
3 |
5 |
NM_130539.2 |
CG11382-RB (CG11382), mRNA |
| 0 |
0 |
0 |
3 |
4 |
NM_139400.1 |
CG8001-RA (CG8001), mRNA |
|
| Wing Disc |
GFP
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Puc
 |
Caspase
 |
DAPI
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Merge
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Figure
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JNK signal
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Caspase-3 signal
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