NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
Stock ID	18374R-3
Symbol	Gyk	Full Name	Glycerol kinase
CG No	CG18374	Old CG No	CG18374
Synonyms	CG18374, Gyk
Accession No (Link to NCBI)	NM_079916.2
Inserted Chr.	lll
Insertional Mutation	lethal
Phenotype induced by Act5C-GAL4 at 28 degrees	viable
Map Viewer
Reference [Please submit your publication]	Swarup S, Pradhan-Sundd T, Verheyen EM. Genome-wide identification of phospho-regulators of Wnt signaling in Drosophila. Development (2015) 142(8) 1502-15 [ PubMed ID = 25852200 ] [ RRC reference ]
Comment
Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
Primer Seq. 5'	aaggcctacatggccggaccgTTCTCCCACAACCGAAACGAC
Primer Seq. 3'	aatctagaggtaccCTCGAAGCCAGCGTAGTTTCA
Predicted Fragment Size	500
IR fragment full Seq	0001 TTCTCCCACA ACCGAAACGA CACCGCCTCC ACCCACGCTA TCTGGTCCAT TCGTTGGGGC 0061 TATTGACGAG GGAACCACTT CCGCGCGATT TATTATATTT CGTGCGGGCA CTGACGAAAT 0121 TGTGTGCTTT CATCAGATTG AAGTGGAGTC TATTTTCCAG AAGGAGGGAT GGTGCGAGCA 0181 GGATCCCATG GCCATAGTGA ATACGGTCAA CGAGTGCATC ACCGGTGCCT GCAAGAAACT 0241 TGTGGCCGTG GGCGGGAAGG TTGAGGAAAT AATCACGATT GGAATAACAA ACCAACGCGA 0301 GTCCACAGTC GTATGGGATA GAAACTCCGG CCAACCGCTG GTGAACGCTA TCATCTGGCT 0361 AGACAATCGT ACCACCAGCA CGGTGGAGGA GCTTTTGGAG ACTATTCCGA ACAATGCCAG 0421 GAACATCAAC TACCTGCGCC CATTGTGCGG ACTTCCTCTG TCTCCGTACT TTTCGGGCGT 0481 GAAACTACGC TGGCTTCGAG
in silico PCR Fragment	0001 TTCTCCCACA ACCGAAACGA CACCGCCTCC ACCCACGCTA TCTGGTCCAT TCGTTGGGGC 0061 TATTGACGAG GGAACCACTT CCGCGCGATT TATTATATTT CGTGCGGGCA CTGACGAAAT 0121 TGTGTGCTTT CATCAGATTG AAGTGGAGTC TATTTTCCAG AAGGAGGGAT GGTGCGAGCA 0181 GGATCCCATG GCCATAGTGA ATACGGTCAA CGAGTGCATC ACCGGTGCCT GCAAGAAACT 0241 TGTGGCCGTG GGCGGGAAGG TTGAGGAAAT AATCACGATT GGAATAACAA ACCAACGCGA 0301 GTCCACAGTC GTATGGGATA GAAACTCCGG CCAACCGCTG GTGAACGCTA TCATCTGGCT 0361 AGACAATCGT ACCACCAGCA CGGTGGAGGA GCTTTTGGAG ACTATTCCGA ACAATGCCAG 0421 GAACATCAAC TACCTGCGCC CATTGTGCGG ACTTCCTCTG TCTCCGTACT TTTCGGGCGT 0481 GAAACTACGC TGGCTTCGAG
Assemble Data
Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
Off-target search result cv : coefficient of variation (0 mm hits / total siRNA count) mm : mismatch

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