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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 1828R-3 
 Symbol dre4  Full Name dre4 
 CG No CG1828  Old CG No CG1828 
 Synonyms Spt16, dSPT16, spt16, dspt16, DRE4/dSPT16, l(3)dre4, l(3)62Bg, CG1828, dre4 
 Accession No (Link to NCBI) NM_057262.2 
 Inserted Chr. lll 
 Insertional Mutation  lethal 
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Neumüller RA, Gross T, Samsonova AA, Vinayagam A, Buckner M, Founk K, Hu Y, Sharifpoor S, Rosebrock AP, Andrews B, Winston F, Perrimon N.
Conserved regulators of nucleolar size revealed by global phenotypic analyses.
Sci Signal (2013) 6(289) ra70 [ PubMed ID = 23962978 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgGACGGACACCATCTCAGTGTT 
 Primer Seq. 3' 
aatctagaggtaccTGGTGTACTTCTTCTCGCCAA 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 NACGGACACC ATCTCAGTGT TTTGCTCGGA TGCCGTGTAT TTTCTCACTA GCAAAAAGAA 
0061 AATAGAATTC CTGAAGCAGA CGCAGAACAT CACCGAAGAA GGTTTTCCGG AAATCAATCT 
0121 GCTTGTTAGG GATCGGACGG ACAAGGACCA GGGCAACTTC GAGAAGCTCA TCAAAGCCCT 
0181 GCAAAACTCG AAGAAGGGCA AGCGTTTGGG CGTCTTCGCC AAGGACGCCT ATCCCGGGGA 
0241 GTTCAGCGAA GCATGGAAGA AATNCCTGAC GGCGTCCAAG TTCGAGCATG TGGACATTAG 
0301 CACTATTATC GCCTACCTCA TGTGTCCCAA GGATGAGTCG GAGATCAACA ACATACGCAA 
0361 GGCATCGCTA GTGTCAATGG ACATTTTCAA CAAGTATTTG AAAGATGAGA TCATGGACAT 
0421 TATTGATTCG GATAGGAAAG TCAAACACAA CAAGCTCTCA GATGGCTGCG AGGCTgNCAT 
0481 TGGCGAGAAG AAGTACACCA  
 in silico PCR Fragment 
0001 GACGGACACC ATCTCAGTGT TTTGCTCGGA TGCCGTGTAT TTTCTCACTA GCAAAAAGAA 
0061 AATAGAATTC CTGAAGCAGA CGCAGAACAT CACCGAAGAA GGTTTTCCGG AAATCAATCT 
0121 GCTTGTTAGG GATCGGACGG ACAAGGACCA GGGCAACTTC GAGAAGCTCA TCAAAGCCCT 
0181 GCAAAACTCG AAGAAGGGCA AGCGTTTGGG CGTCTTCGCC AAGGACGCCT ATCCCGGGGA 
0241 GTTCAGCGAA GCATGGAAGA AATCCCTGAC GGCGTCCAAG TTCGAGCATG TGGACATTAG 
0301 CACTATTATC GCCTACCTCA TGTGTCCCAA GGATGAGTCG GAGATCAACA ACATACGCAA 
0361 GGCATCGCTA GTGTCAATGG ACATTTTCAA CAAGTATTTG AAAGATGAGA TCATGGACAT 
0421 TATTGATTCG GATAGGAAAG TCAAACACAA CAAGCTCTCA GATGGCTGCG AGGCTGCCAT 
0481 TGGCGAGAAG AAGTACACCA  
 Assemble Data 

1828R-3.IR_full        1   ACGGACACCATCTCAGTGTTTTGCTCGGATGCCGTGTATTTTCTCACTAGCAAAAAGAA 59
                           |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1828R-3.in silico      1   ACGGACACCATCTCAGTGTTTTGCTCGGATGCCGTGTATTTTCTCACTAGCAAAAAGAA 59


1828R-3.IR_full       61  AATAGAATTCCTGAAGCAGACGCAGAACATCACCGAAGAAGGTTTTCCGGAAATCAATCT 119
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1828R-3.in silico     61  AATAGAATTCCTGAAGCAGACGCAGAACATCACCGAAGAAGGTTTTCCGGAAATCAATCT 119


1828R-3.IR_full       121 GCTTGTTAGGGATCGGACGGACAAGGACCAGGGCAACTTCGAGAAGCTCATCAAAGCCCT 179
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1828R-3.in silico     121 GCTTGTTAGGGATCGGACGGACAAGGACCAGGGCAACTTCGAGAAGCTCATCAAAGCCCT 179


1828R-3.IR_full       181 GCAAAACTCGAAGAAGGGCAAGCGTTTGGGCGTCTTCGCCAAGGACGCCTATCCCGGGGA 239
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1828R-3.in silico     181 GCAAAACTCGAAGAAGGGCAAGCGTTTGGGCGTCTTCGCCAAGGACGCCTATCCCGGGGA 239


1828R-3.IR_full       241 GTTCAGCGAAGCATGGAAGAAATNCCTGACGGCGTCCAAGTTCGAGCATGTGGACATTAG 299
                          ||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||||
1828R-3.in silico     241 GTTCAGCGAAGCATGGAAGAAATCCCTGACGGCGTCCAAGTTCGAGCATGTGGACATTAG 299


1828R-3.IR_full       301 CACTATTATCGCCTACCTCATGTGTCCCAAGGATGAGTCGGAGATCAACAACATACGCAA 359
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1828R-3.in silico     301 CACTATTATCGCCTACCTCATGTGTCCCAAGGATGAGTCGGAGATCAACAACATACGCAA 359


1828R-3.IR_full       361 GGCATCGCTAGTGTCAATGGACATTTTCAACAAGTATTTGAAAGATGAGATCATGGACAT 419
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1828R-3.in silico     361 GGCATCGCTAGTGTCAATGGACATTTTCAACAAGTATTTGAAAGATGAGATCATGGACAT 419


1828R-3.IR_full       421 TATTGATTCGGATAGGAAAGTCAAACACAACAAGCTCTCAGATGGCTGCGAGGCTGNCAT 479
                          |||||||||||||||||||||||||||||||||||||||||||||||||||||||| |||
1828R-3.in silico     421 TATTGATTCGGATAGGAAAGTCAAACACAACAAGCTCTCAGATGGCTGCGAGGCTGCCAT 479


1828R-3.IR_full       481 TGGCGAGAAGAAGTACACCA 499
                          ||||||||||||||||||||
1828R-3.in silico     481 TGGCGAGAAGAAGTACACCA 499



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  1  NM_167922.1  CG1828-RB, transcript variant B (dre4), mRNA 
100   482  0  0  1  NM_057262.2  CG1828-RA, transcript variant A (dre4), mRNA 
0   0  2  4  5  NM_167558.1  CG32562-RA (xmas-2), mRNA 
0   0  2  3  2  NM_139507.2  CG16753-RA (CG16753), mRNA 
0   0  2  2  2  NM_141907.2  CG10014-RA (CG10014), mRNA 
0   0  1  4  3  NM_079060.2  CG17725-RA, transcript variant A (Pepck), mRNA 
0   0  1  4  3  NM_166293.1  CG17725-RB, transcript variant B (Pepck), mRNA 
0   0  1  4  2  NM_130567.2  CG14782-RA (CG14782), mRNA 
0   0  1  3  5  NM_143407.2  CG11305-RA (Sirt7), mRNA 
0   0  1  3  4  NM_169024.1  CG31535-RA (CG31535), mRNA 
0   0  1  3  3  NM_137312.2  CG5348-RA (CG5348), mRNA 
0   0  1  3  2  NM_078548.2  CG2985-RA (Yp1), mRNA 
0   0  1  2  5  NM_058047.3  CG7961-RA, transcript variant A (alphaCop), mRNA 
0   0  1  2  5  NM_167908.1  CG7961-RB, transcript variant B (alphaCop), mRNA 
0   0  1  2  5  NM_078654.2  CG9623-RB, transcript variant B (if), mRNA 
0   0  1  2  5  NM_167544.1  CG9623-RA, transcript variant A (if), mRNA 
0   0  1  2  3  NM_176591.1  CG15532-RC, transcript variant C (hdc), mRNA 
0   0  1  2  3  NM_079853.2  CG15532-RA, transcript variant A (hdc), mRNA 
0   0  1  2  2  NM_135115.2  CG14005-RA (CG14005), mRNA 
0   0  0  6  6  NM_135156.2  CG13991-RA (CG13991), mRNA 
0   0  0  5  4  NM_164328.2  CG32490-RG, transcript variant G (cpx), mRNA 
0   0  0  5  4  NM_164329.2  CG32490-RI, transcript variant I (cpx), mRNA 
0   0  0  5  4  NM_164331.2  CG32490-RH, transcript variant H (cpx), mRNA 
0   0  0  5  4  NM_164330.2  CG32490-RC, transcript variant C (cpx), mRNA 
0   0  0  5  4  NM_164327.2  CG32490-RA, transcript variant A (cpx), mRNA 
0   0  0  5  4  NM_001014603.1  CG32490-RJ, transcript variant J (cpx), mRNA 
0   0  0  5  4  NM_001014602.1  CG32490-RK, transcript variant K (cpx), mRNA 
0   0  0  5  2  NM_057882.3  CG1609-RA (Gcn2), mRNA 
0   0  0  5  2  NM_131934.1  CG3626-RA (CG3626), mRNA 
0   0  0  4  5  NM_138249.2  CG9139-RA (CG9139), mRNA 

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