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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 18265R-2 
 Symbol CG18265  Full Name CG18265 
 CG No CG18265  Old CG No CG18265 
 Synonyms CG18265 
 Accession No (Link to NCBI) NM_140725.1 
 Inserted Chr. lll 
 Insertional Mutation  lethal 
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgGACGTCTCCAACTCATCGACA 
 Primer Seq. 3' 
aatctagaggtaccGGAAGGAACATCTTCGACAAC 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 NACGTCTCCA ACTCATCGAC ACTCAACACG AACAGGAACA ACCACACCAG CGGCAGcaAC 
0061 AGCAACAATC CACATGAAGC CGCaNGAGCA ACACCCACAG CCACGTCAGC CACAACCAGA 
0121 GGAACAACAG GAGCAGGAGG AGTGGAAAGC ACTTCACCCG CCGACCATTT GGTGCCGGCA 
0181 ATGGAGCTGG AACTGGACCC GAAGCCAGCG GGAAGCGGAG CVAATGAGGC GGACCAATTG 
0241 GAGGAGGCAG AGGATGGACG CAAAGATGTG GATGCAGAGG AGGAGGTGGG CGATgCCAAC 
0301 ATGGCCACCA TGGCGgcAGC CATGCAGCTG CAGATACTGG AGGCCCTAAG TGATGCGGCC 
0361 AAGAAGCGAC GCAAGCAGCA CAATCCCAGC CGCTTAGAGG CGCTCAATTT AAGTGGGGAA 
0421 CCCACTGGTG AAGTGGGTCA AAAGAAAGCA GACTCCTCCA CCGTGGACTA CGAGGACAAG 
0481 TTGTCGAAGA TGTTcCTTCC  
 in silico PCR Fragment 
0001 GACGTCTCCA ACTCATCGAC ACTCAACACG AACAGGAACA ACCACACCAG CGGCAGCAAC 
0061 AGCAACAATC CACATGAAGC CGCAGGAGCA ACACCCACAG CCACGTCAGC CACAACCAGA 
0121 GGAGCAACAG GAGCAGGAGG AGTGGAAAGC ACTTCACCCG CCGACCATTT GGTGCCGGCA 
0181 ATGGAGCTGG AACTGGACCC GAAGCCAGCG GGAAGCGGAG CGAATGAGGC GGACCAATTG 
0241 GAGGAGGCAG AAGATGGACG CAAAGATGTG GATGCAGAGG AGGAGGTGGG CGATGCCAAC 
0301 ATGGCCACCA TGGCGGCAGC CATGCAGCTG CAGATACTGG AGGCCCTAAG TGATGCGGCC 
0361 AAGAAGCGAC GCAAGCAGCA CAATCCCAGC CGCTTAGAGG CGCTCAATTT AAGTGGGGAA 
0421 CCCACTGGTG AAGTGGGTCA AAAGAAAGCA GACTCCTCCA CCGTGGACTA CGAGGACAAG 
0481 TTGTCGAAGA TGTTCCTTCC  
 Assemble Data 

18265R-2.IR_full        1   ACGTCTCCAACTCATCGACACTCAACACGAACAGGAACAACCACACCAGCGGCAGCAAC 59
                            |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18265R-2.in silico      1   ACGTCTCCAACTCATCGACACTCAACACGAACAGGAACAACCACACCAGCGGCAGCAAC 59


18265R-2.IR_full       61  AGCAACAATCCACATGAAGCCGCANGAGCAACACCCACAGCCACGTCAGCCACAACCAGA 119
                           |||||||||||||||||||||||| |||||||||||||||||||||||||||||||||||
18265R-2.in silico     61  AGCAACAATCCACATGAAGCCGCAGGAGCAACACCCACAGCCACGTCAGCCACAACCAGA 119


18265R-2.IR_full       121 GGAACAACAGGAGCAGGAGGAGTGGAAAGCACTTCACCCGCCGACCATTTGGTGCCGGCA 179
                           ||| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18265R-2.in silico     121 GGAGCAACAGGAGCAGGAGGAGTGGAAAGCACTTCACCCGCCGACCATTTGGTGCCGGCA 179


18265R-2.IR_full       181 ATGGAGCTGGAACTGGACCCGAAGCCAGCGGGAAGCGGAGCVAATGAGGCGGACCAATTG 239
                           ||||||||||||||||||||||||||||||||||||||||| ||||||||||||||||||
18265R-2.in silico     181 ATGGAGCTGGAACTGGACCCGAAGCCAGCGGGAAGCGGAGCGAATGAGGCGGACCAATTG 239


18265R-2.IR_full       241 GAGGAGGCAGAGGATGGACGCAAAGATGTGGATGCAGAGGAGGAGGTGGGCGATGCCAAC 299
                           ||||||||||| ||||||||||||||||||||||||||||||||||||||||||||||||
18265R-2.in silico     241 GAGGAGGCAGAAGATGGACGCAAAGATGTGGATGCAGAGGAGGAGGTGGGCGATGCCAAC 299


18265R-2.IR_full       301 ATGGCCACCATGGCGGCAGCCATGCAGCTGCAGATACTGGAGGCCCTAAGTGATGCGGCC 359
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18265R-2.in silico     301 ATGGCCACCATGGCGGCAGCCATGCAGCTGCAGATACTGGAGGCCCTAAGTGATGCGGCC 359


18265R-2.IR_full       361 AAGAAGCGACGCAAGCAGCACAATCCCAGCCGCTTAGAGGCGCTCAATTTAAGTGGGGAA 419
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18265R-2.in silico     361 AAGAAGCGACGCAAGCAGCACAATCCCAGCCGCTTAGAGGCGCTCAATTTAAGTGGGGAA 419


18265R-2.IR_full       421 CCCACTGGTGAAGTGGGTCAAAAGAAAGCAGACTCCTCCACCGTGGACTACGAGGACAAG 479
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18265R-2.in silico     421 CCCACTGGTGAAGTGGGTCAAAAGAAAGCAGACTCCTCCACCGTGGACTACGAGGACAAG 479


18265R-2.IR_full       481 TTGTCGAAGATGTTCCTTCC 499
                           ||||||||||||||||||||
18265R-2.in silico     481 TTGTCGAAGATGTTCCTTCC 499



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  1  5  NM_140725.1  CG18265-RA (CG18265), mRNA 
0.41   2  2  4  5  NM_137429.2  CG11430-RB, transcript variant B (olf186-F), mRNA 
0.41   2  2  4  5  NM_170626.1  CG11430-RA, transcript variant A (olf186-F), mRNA 
0.41   2  2  3  16  NM_168301.1  CG5735-RD, transcript variant D (orb2), mRNA 
0.41   2  2  3  16  NM_140009.1  CG5735-RB, transcript variant B (orb2), mRNA 
0.41   2  2  3  16  NM_168300.1  CG5735-RC, transcript variant C (orb2), mRNA 
0.41   2  2  2  2  NM_170627.1  CG11430-RC, transcript variant C (olf186-F), mRNA 
0.2   1  4  9  39  NM_165242.1  CG7210-RA, transcript variant A (kel), mRNA 
0.2   1  4  9  39  NM_057241.2  CG7210-RB, transcript variant B (kel), mRNA 
0.2   1  4  3  27  NM_168038.1  CG32264-RD, transcript variant D (CG32264), mRNA 
0.2   1  3  8  26  NM_142755.1  CG5732-RA (CG5732), mRNA 
0.2   1  2  13  54  NM_132606.1  CG3754-RA (CG3754), mRNA 
0.2   1  2  2  3  NM_134676.1  CG4213-RA (CG4213), mRNA 
0   0  9  17  103  NM_001038965.1  CG9924-RE, transcript variant E (CG9924), mRNA 
0   0  9  15  74  NM_132569.1  CG11245-RA (CG11245), mRNA 
0   0  9  6  26  NM_136645.1  CG13952-RA (CG13952), mRNA 
0   0  8  21  90  NM_142597.2  CG12254-RA (MED25), mRNA 
0   0  8  11  43  NM_168239.1  CG17888-RC, transcript variant C (Pdp1), mRNA 
0   0  7  36  134  NM_169783.1  CG31243-RB, transcript variant B (cpo), mRNA 
0   0  7  36  133  NM_169781.1  CG31243-RE, transcript variant E (cpo), mRNA 
0   0  7  36  133  NM_169782.1  CG31243-RA, transcript variant A (cpo), mRNA 
0   0  7  23  57  NM_080025.1  CG11387-RA, transcript variant A (ct), mRNA 
0   0  6  35  158  NM_078797.2  CG13109-RA (tai), mRNA 
0   0  6  30  95  NM_080105.2  CG31243-RF, transcript variant F (cpo), mRNA 
0   0  6  24  74  NM_176745.1  CG5424-RD, transcript variant D (f), mRNA 
0   0  6  24  74  NM_001042819.1  CG5424-RF, transcript variant F (f), mRNA 
0   0  6  24  74  NM_078660.2  CG5424-RB, transcript variant B (f), mRNA 
0   0  6  24  72  NM_176746.1  CG5424-RC, transcript variant C (f), mRNA 
0   0  6  4  29  NM_001014582.1  CG10488-RB, transcript variant B (eyg), mRNA 
0   0  6  4  29  NM_079318.2  CG10488-RA, transcript variant A (eyg), mRNA 

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