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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
18255R-1 |
| Symbol |
Strn-Mlck |
Full Name |
Stretchin-Mlck |
| CG No |
CG18255 |
Old CG No |
CG18255 |
| Synonyms |
143736_at, A(225), CT41348, MLCK, mlck, CG18255, unnamed, Mlc-k, CG8304, MLCK-2, MLCK-3, MLCK-1, Mlck, BEST:GH22543, BcDNA:RH74685, Strn-Mlck |
| Accession No (Link to NCBI) |
NM_166125.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
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Reference
[Please submit your publication]
|
Jang YG, Choi Y, Jun K, Chung J.
Mislocalization of TORC1 to Lysosomes Caused by KIF11 Inhibition Leads to Aberrant TORC1 Activity.
Mol Cells (2020) 43(8) 705-717
[
PubMed ID = 32759469
]
[
RRC reference
]
Parsons LM, Grzeschik NA, Amaratunga K, Burke P, Quinn LM, Richardson HE.
A Kinome RNAi Screen in Drosophila Identifies Novel Genes Interacting with Lgl, aPKC, and Crb Cell Polarity Genes in Epithelial Tissues.
G3 (Bethesda) (2017) 7(8) 2497-2509
[
PubMed ID = 28611255
]
[
RRC reference
]
Swarup S, Pradhan-Sundd T, Verheyen EM.
Genome-wide identification of phospho-regulators of Wnt signaling in Drosophila.
Development (2015) 142(8) 1502-15
[
PubMed ID = 25852200
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgTGCCAGTGCAATGAGCACAAG |
| Primer Seq. 3' |
aatctagaggtaccAAGTTCTCCGCCATTAGCATC |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 TGCCAGTGCA ATGAGCACAA GCAACTCAAC GGCACACTCT CCTCGGCCTC GTCGGTCTAC
0061 AGGAAAAGGA GCCGAGGTGA GATTCCCATT GAAAATTCCG ATCGCTTCCG CATCACGGCC
0121 ACCAGCAATG CCGTCCAGCT GGCCGTGGAG CATGTGCAGC GGGAAGACGC CGGCCACTAC
0181 ACGCTGTTCG CCCGGACGAA GAGGCAGGAT GTGGTGCGCA GGCACGTCGA GCTCATCGTG
0241 GAGGACAGAT CCACAGGCGA CGATCCGCCG GTGTTCGTGC GCCGCCTGCC CGACCTATCC
0301 GTCAAAGTGG GCACCCGGAC ACGCCTCCTA ACCGAAATAC GCAGCTCAAC AGATCTTAAG
0361 CTAACCTGGG TACAGAAATG ATCGGAGAGT GTGCGCAAAC GACCGGATCA CGGAAGTCAA
0421 TGAAGGCACC TTCCACTACT TGGAGATTAG TCCCGTGACG CCTGGACGAC GGTGGTCAGT
0481 GGATGCTAAT GGCGGAGAAC TT |
| in silico PCR Fragment |
0001 TGCCAGTGCA ATGAGCACAA GCAACTCAAC GGCACACTCT CCTCGGCCTC GTCGGTCTAC
0061 AGGAAAAGGA GCCGAGGTGA GATTCCCATT GAAAATTCCG ATCGCTTCCG CATCACGGCC
0121 ACCAGCAATG CCGTCCAGCT GGCCGTGGAG CATGTGCAGC GGGAAGACGC CGGCCACTAC
0181 ACGCTGTTCG CCCGGACGAA GAGGCAGGAT GTGGTGCGCA GGCACGTCGA GCTCATCGTG
0241 GAGGACAGAT CCACGGGCGA CGATCCGCCG GTGTTCGTGC GCCGCCTGCC CGACCTATCC
0301 GTCAAAGTGG GCACCCGGAC ACGCCTCCTA ACCGAAATAC GCAGCTCAAC AGATCTTAAG
0361 CTAACCT-GG TACAGAAATG ATCGGAGAGT GTGCGCAAAC GACCGGATCA CGGAAGTCAA
0421 TGAAGGCACC TTCCACTACT TGGAGATTAG TCCCGTGACG -CTGGACGAC GGTGGTCAGT
0481 GGATGCTAAT GGCGGAGAAC TT |
| Assemble Data |
18255R-1.IR_full 1 TGCCAGTGCAATGAGCACAAGCAACTCAACGGCACACTCTCCTCGGCCTCGTCGGTCTAC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18255R-1.in silico 1 TGCCAGTGCAATGAGCACAAGCAACTCAACGGCACACTCTCCTCGGCCTCGTCGGTCTAC 60
18255R-1.IR_full 61 AGGAAAAGGAGCCGAGGTGAGATTCCCATTGAAAATTCCGATCGCTTCCGCATCACGGCC 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18255R-1.in silico 61 AGGAAAAGGAGCCGAGGTGAGATTCCCATTGAAAATTCCGATCGCTTCCGCATCACGGCC 120
18255R-1.IR_full 121 ACCAGCAATGCCGTCCAGCTGGCCGTGGAGCATGTGCAGCGGGAAGACGCCGGCCACTAC 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18255R-1.in silico 121 ACCAGCAATGCCGTCCAGCTGGCCGTGGAGCATGTGCAGCGGGAAGACGCCGGCCACTAC 180
18255R-1.IR_full 181 ACGCTGTTCGCCCGGACGAAGAGGCAGGATGTGGTGCGCAGGCACGTCGAGCTCATCGTG 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18255R-1.in silico 181 ACGCTGTTCGCCCGGACGAAGAGGCAGGATGTGGTGCGCAGGCACGTCGAGCTCATCGTG 240
18255R-1.IR_full 241 GAGGACAGATCCACAGGCGACGATCCGCCGGTGTTCGTGCGCCGCCTGCCCGACCTATCC 300
|||||||||||||| |||||||||||||||||||||||||||||||||||||||||||||
18255R-1.in silico 241 GAGGACAGATCCACGGGCGACGATCCGCCGGTGTTCGTGCGCCGCCTGCCCGACCTATCC 300
18255R-1.IR_full 301 GTCAAAGTGGGCACCCGGACACGCCTCCTAACCGAAATACGCAGCTCAACAGATCTTAAG 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18255R-1.in silico 301 GTCAAAGTGGGCACCCGGACACGCCTCCTAACCGAAATACGCAGCTCAACAGATCTTAAG 360
18255R-1.IR_full 361 CTAACCTGGGTACAGAAATGATCGGAGAGTGTGCGCAAACGACCGGATCACGGAAGTCAA 420
||||||| ||||||||||||||||||||||||||||||||||||||||||||||||||||
18255R-1.in silico 361 CTAACCT-GGTACAGAAATGATCGGAGAGTGTGCGCAAACGACCGGATCACGGAAGTCAA 420
18255R-1.IR_full 421 TGAAGGCACCTTCCACTACTTGGAGATTAGTCCCGTGACGCCTGGACGACGGTGGTCAGT 480
|||||||||||||||||||||||||||||||||||||||| |||||||||||||||||||
18255R-1.in silico 421 TGAAGGCACCTTCCACTACTTGGAGATTAGTCCCGTGACG-CTGGACGACGGTGGTCAGT 480
18255R-1.IR_full 481 GGATGCTAATGGCGGAGAACTT 502
||||||||||||||||||||||
18255R-1.in silico 481 GGATGCTAATGGCGGAGAACTT 502
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
7 |
NM_166125.2 |
CG18255-RA, transcript variant A (Strn-Mlck), mRNA |
| 100 |
482 |
0 |
0 |
6 |
NM_166129.2 |
CG18255-RD, transcript variant D (Strn-Mlck), mRNA |
| 0 |
0 |
3 |
4 |
2 |
NM_141004.1 |
CG3680-RA (CG3680), mRNA |
| 0 |
0 |
3 |
4 |
2 |
NM_130560.2 |
CG14789-RA (O-fut2), mRNA |
| 0 |
0 |
2 |
5 |
4 |
NM_137509.2 |
CG5482-RA (CG5482), mRNA |
| 0 |
0 |
2 |
3 |
7 |
NM_169499.1 |
CG8449-RA (CG8449), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_142188.2 |
CG6276-RA (CG6276), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_140872.1 |
CG9330-RA (CG9330), mRNA |
| 0 |
0 |
1 |
6 |
3 |
NM_170053.2 |
CG17894-RC, transcript variant C (cnc), mRNA |
| 0 |
0 |
1 |
6 |
2 |
NM_170054.2 |
CG17894-RB, transcript variant B (cnc), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_167831.1 |
CG1228-RD, transcript variant D (Ptpmeg), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_167830.1 |
CG1228-RA, transcript variant A (Ptpmeg), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_138187.2 |
CG1228-RC, transcript variant C (Ptpmeg), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_166913.1 |
CG32801-RA (CG32801), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_165581.1 |
CG11198-RB, transcript variant B (CG11198), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_136498.1 |
CG11198-RA, transcript variant A (CG11198), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_001032051.1 |
CG33715-RD, transcript variant D (Msp-300), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_140906.1 |
CG14182-RA (CG14182), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_001032053.1 |
CG33715-RB, transcript variant B (Msp-300), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_167327.1 |
CG1848-RD, transcript variant D (LIMK1), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_167326.1 |
CG1848-RA, transcript variant A (LIMK1), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_078584.2 |
CG1848-RC, transcript variant C (LIMK1), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_170368.1 |
CG31051-RA (CG31051), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_136656.2 |
CG1868-RA, transcript variant A (CG1868), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_165681.1 |
CG1868-RB, transcript variant B (CG1868), mRNA |
| 0 |
0 |
0 |
6 |
4 |
NM_142695.1 |
CG5810-RA (CG5810), mRNA |
| 0 |
0 |
0 |
6 |
3 |
NM_080516.1 |
CG16757-RA (Spn), mRNA |
| 0 |
0 |
0 |
5 |
6 |
NM_140803.1 |
CG14073-RB, transcript variant B (CG14073), mRNA |
| 0 |
0 |
0 |
5 |
5 |
NM_057278.3 |
CG7727-RA (Appl), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_139439.2 |
CG15820-RA (CG15820), mRNA |
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