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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 1822R-3 
 Symbol bif  Full Name bifocal 
 CG No CG1822  Old CG No CG1822 
 Synonyms CG1822, bif 
 Accession No (Link to NCBI) NM_167295.1 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgATGGAGTCACAGAAGCGGCCT 
 Primer Seq. 3' 
aatctagaggtaccATCTCCCTCTCCTGACTTCTT 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 aTGGAGTCAC AGAAGCGGCC TTCGTTGGAC TTACACACGG ATGTGCCAGC AGGATTAGCA 
0061 GCTGGAGGAT CTGGCCTGGG AGCTGCAGCT GAGATGTCGC CCACTTCCGG TTTCCTGCCG 
0121 GACATGCCGC AGTGGAAGAA GGACCTCATC CAGCGCCGGA AAACGAACGT GGCCCGCACC 
0181 CAGGCGGCGT CCATCACCTC GCCCACCGAT GGCAGTTGTG GGGCTTTGGC AGANAGCCAA 
0241 CGCAGCTCCA GGTGCAATTG CAGATTTCAC AGAACCGGCG ACAATCAGTA GCACTAGTCA 
0301 AAAGAGAAAC ATGATCGGTT CAGAGGAAAA GTCTGAGAAA TCTTCTATTT CCAATACCAA 
0361 TTCCGATTCC ACTGGAGGTC ATCACTCTGT TGTTGCCGTC TCCCTTTCGC CCGATGCGGC 
0421 AGCAACAACA AATGTAACAG TAACACCAAT ACCAAAGCAG CGATCGAGTT TACTCAACAC 
0481 AAGAAGTCAG GAGAGGGAGA T 
 in silico PCR Fragment 
0001 ATGGAGTCAC AGAAGCGGCC TTCGTTGGAC TTACACACGG ATGTGCCAGC AGGATTAGCA 
0061 GCTGGAGGAT CTGGCCTGGG AGCTGCAGCT GAGATGTCGC CCACTTCCGG TTTCCTGCCG 
0121 GACATGCCGC AGTGGAAGAA GGACCTCATC CAGCGCCGGA AAACGAACGT GGCCCGCACC 
0181 CAGGCGGCGT CCATCACCTC GCCCACCGAT GGCAGTTGTG GGGCTTTGGC AGA-AGCCAA 
0241 CGCAGCTCCA GGTGCAATTG CAGATTTCAC AGAACCGGCG ACAATCAGTA GCACTAGTCA 
0301 AAAGAGAAAC ATGATCGGTT CAGAGGAAAA GTCTGAGAAA TCTTCTATTT CCAATACCAA 
0361 TTCCGATTCC ACTGGAGGTC ATCACTCTGT TGTTGCCGTC TCCCTTTCGC CCGATGCGGC 
0421 AGCAACAACA AATGTAACAG TAACACCAAT ACCAAAGCAG CGATCGAGTT TACTCAACAC 
0481 AAGAAGTCAG GAGAGGGAGA T 
 Assemble Data 

1822R-3.IR_full       1   ATGGAGTCACAGAAGCGGCCTTCGTTGGACTTACACACGGATGTGCCAGCAGGATTAGCA 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1822R-3.in silico     1   ATGGAGTCACAGAAGCGGCCTTCGTTGGACTTACACACGGATGTGCCAGCAGGATTAGCA 60


1822R-3.IR_full       61  GCTGGAGGATCTGGCCTGGGAGCTGCAGCTGAGATGTCGCCCACTTCCGGTTTCCTGCCG 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1822R-3.in silico     61  GCTGGAGGATCTGGCCTGGGAGCTGCAGCTGAGATGTCGCCCACTTCCGGTTTCCTGCCG 120


1822R-3.IR_full       121 GACATGCCGCAGTGGAAGAAGGACCTCATCCAGCGCCGGAAAACGAACGTGGCCCGCACC 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1822R-3.in silico     121 GACATGCCGCAGTGGAAGAAGGACCTCATCCAGCGCCGGAAAACGAACGTGGCCCGCACC 180


1822R-3.IR_full       181 CAGGCGGCGTCCATCACCTCGCCCACCGATGGCAGTTGTGGGGCTTTGGCAGANAGCCAA 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||| ||||||
1822R-3.in silico     181 CAGGCGGCGTCCATCACCTCGCCCACCGATGGCAGTTGTGGGGCTTTGGCAGA-AGCCAA 240


1822R-3.IR_full       241 CGCAGCTCCAGGTGCAATTGCAGATTTCACAGAACCGGCGACAATCAGTAGCACTAGTCA 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1822R-3.in silico     241 CGCAGCTCCAGGTGCAATTGCAGATTTCACAGAACCGGCGACAATCAGTAGCACTAGTCA 300


1822R-3.IR_full       301 AAAGAGAAACATGATCGGTTCAGAGGAAAAGTCTGAGAAATCTTCTATTTCCAATACCAA 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1822R-3.in silico     301 AAAGAGAAACATGATCGGTTCAGAGGAAAAGTCTGAGAAATCTTCTATTTCCAATACCAA 360


1822R-3.IR_full       361 TTCCGATTCCACTGGAGGTCATCACTCTGTTGTTGCCGTCTCCCTTTCGCCCGATGCGGC 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1822R-3.in silico     361 TTCCGATTCCACTGGAGGTCATCACTCTGTTGTTGCCGTCTCCCTTTCGCCCGATGCGGC 420


1822R-3.IR_full       421 AGCAACAACAAATGTAACAGTAACACCAATACCAAAGCAGCGATCGAGTTTACTCAACAC 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1822R-3.in silico     421 AGCAACAACAAATGTAACAGTAACACCAATACCAAAGCAGCGATCGAGTTTACTCAACAC 480


1822R-3.IR_full       481 AAGAAGTCAGGAGAGGGAGAT 501
                          |||||||||||||||||||||
1822R-3.in silico     481 AAGAAGTCAGGAGAGGGAGAT 501



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  2  NM_078573.2  CG1822-RC, transcript variant C (bif), mRNA 
100   482  0  0  2  NM_167295.1  CG1822-RB, transcript variant B (bif), mRNA 
0   0  6  2  2  NM_144140.1  CG2249-RA, transcript variant A (CG2249), mRNA 
0   0  6  2  2  NM_176118.1  CG2249-RB, transcript variant B (CG2249), mRNA 
0   0  4  4  10  NM_141823.2  CG5308-RA, transcript variant A (dpr5), mRNA 
0   0  3  5  7  NM_130689.1  CG17959-RA (CG17959), mRNA 
0   0  3  4  4  NM_140007.1  CG5747-RA (mfr), mRNA 
0   0  3  2  4  NM_142493.2  CG7717-RA, transcript variant A (Mekk1), mRNA 
0   0  3  2  4  NM_169833.1  CG7717-RB, transcript variant B (Mekk1), mRNA 
0   0  3  2  2  NM_176544.1  CG33193-RA (sav), mRNA 
0   0  2  4  8  NM_079445.2  CG8567-RA, transcript variant A (Deaf1), mRNA 
0   0  2  4  8  NM_001038944.1  CG8567-RB, transcript variant B (Deaf1), mRNA 
0   0  2  3  2  NM_136060.1  CG10348-RA (CG10348), mRNA 
0   0  2  2  3  NM_142797.1  CG5376-RA (CG5376), mRNA 
0   0  1  7  15  NM_078740.2  CG8817-RB, transcript variant B (lilli), mRNA 
0   0  1  7  15  NM_164516.1  CG8817-RA, transcript variant A (lilli), mRNA 
0   0  1  7  15  NM_164517.1  CG8817-RC, transcript variant C (lilli), mRNA 
0   0  1  5  7  NM_140602.1  CG13050-RA (CG13050), mRNA 
0   0  1  4  5  NM_139717.1  CG5146-RA (CG5146), mRNA 
0   0  1  4  5  NM_141178.3  CG12581-RA, transcript variant A (CG12581), mRNA 
0   0  1  4  5  NM_164313.1  CG12581-RB, transcript variant B (CG12581), mRNA 
0   0  1  3  8  NM_001042893.1  CG5461-RE, transcript variant E (bun), mRNA 
0   0  1  3  5  NM_079571.1  CG8374-RA (dmt), mRNA 
0   0  1  3  5  NM_170146.1  CG6844-RB, transcript variant B (nAcRalpha-96Ab), mRNA 
0   0  1  3  5  NM_079758.2  CG6844-RA, transcript variant A (nAcRalpha-96Ab), mRNA 
0   0  1  3  3  NM_140521.1  CG6498-RA (CG6498), mRNA 
0   0  1  3  3  NM_135567.2  CG6187-RA (RluA-2), mRNA 
0   0  1  3  3  NM_137200.2  CG30467-RA (CG30467), mRNA 
0   0  1  3  2  NM_057884.4  CG3478-RA (ppk), mRNA 
0   0  1  2  5  NM_165453.1  CG14471-RA, transcript variant A (CG14471), mRNA 
 Wing Disc
GFP
 Puc
 Caspase
DAPI
 Merge
 Figure
JNK signal
N
 
DB
 
DC
 
DM
 
VM
 
VB
 
Caspase-3 signal
N
++ 
DB
+ 
DC
+ 
DM
 
VM
 
VB
 
Dcompartment   Penetrance
     

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