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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 1815R-3 
 Symbol CG1815  Full Name CG1815 
 CG No CG1815  Old CG No CG1815 
 Synonyms CG1815 
 Accession No (Link to NCBI) NM_170562.1 
 Inserted Chr. ll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Hosono C, Matsuda R, Adryan B, Samakovlis C.
Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes.
Nat Cell Biol (2015) 17(12) 1569-76 [ PubMed ID = 26551273 ] [ RRC reference ]

Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgATGGAAAGCACGGACAGCAGC 
 Primer Seq. 3' 
aatctagaggtaccGAGGTATTTAAGCTGCGGGAC 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 ATGGAAAGCA CGGACAGCAG CGACAGCAGT GGGGATAGCT TAAAATCCAT TCCCCGGCCG 
0061 GACTTTGAGG CCCTCTCCGC CATCGGCAGC NCCACAGCCG CAGTTGCAGA NCTCCTCGCT 
0121 GGATGGCCAC GTCGAGATGG AGCCGGTCAT CATGCCCAGC AAGCGTCAAA AGTATATTGT 
0181 CAGTGCTCCG CCNTGGGTCG GCCAACTGCA GCAGTAACTC ATCCAGTTCG AGTCCCTCGA 
0241 ACATCAACGT GGGCTCCTCG CTTACCATGA AGTTGACGAA GGTGCAGCCA CAACAGCATC 
0301 AGGCTAAGTC CTCCACGCCA AACAACAAAG GCAAAGTAGC CAAGGAAATG GTTGCTCTGC 
0361 AGCGCTCACA CATCGAATCC GAGGTCCTCA GCAACTTTGT GACTGGGGTG TCCAAGCTGA 
0421 AGAGTCGAAA GTCGCGATAT GTGCCCGGGA ATCTCGCCAC TCAGGCAGTA GGTGGAGAGC 
0481 TGTCCCGCAG CTTAAATACC TC 
 in silico PCR Fragment 
0001 ATGGAAAGCA CGGACAGCAG CGACAGCAGT GGGGATAGCT TAAAATCCAT TCCCCGGCCG 
0061 GACTTTGAGG CCCTCTCCGC CATCGGCAGC -CCACAGCCG CAGTTGCAGA GCTCCTCGCT 
0121 GGATGGCCAC GTCGAGATGG AGCCGGTCAT CATGCCCAGC AAGCGTCAAA AGTATATTGT 
0181 CAGTGCTCCG CC-TGGGTCG GCCAACTGCA GCAGTAACTC ATCCAGTTCG AGTCCCTCGA 
0241 ACATCAACGT GGGCTCCTCG CTTACCATGA AGTTGACGAA GGTGCAGCCA CAACAGCATC 
0301 AGGCTAAGTC CTCCACGCCA AACAACAAAG GCAAAGTAGC CAAGGAAATG GTTGCTCTGC 
0361 AGCGCTCACA CATCGAATCC GAGGTCCTCA GCAACTTTGT GACTGGGGTG TCCAAGCTGA 
0421 AGAGTCGAAA GTCGCGATAT GTGCCCGGGA ATCTCGCCAC TCAGGCAGTA GGTGGAGAGC 
0481 TGTCCCGCAG CTTAAATACC TC 
 Assemble Data 

1815R-3.IR_full       1   ATGGAAAGCACGGACAGCAGCGACAGCAGTGGGGATAGCTTAAAATCCATTCCCCGGCCG 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1815R-3.in silico     1   ATGGAAAGCACGGACAGCAGCGACAGCAGTGGGGATAGCTTAAAATCCATTCCCCGGCCG 60


1815R-3.IR_full       61  GACTTTGAGGCCCTCTCCGCCATCGGCAGCNCCACAGCCGCAGTTGCAGANCTCCTCGCT 120
                          |||||||||||||||||||||||||||||| ||||||||||||||||||| |||||||||
1815R-3.in silico     61  GACTTTGAGGCCCTCTCCGCCATCGGCAGC-CCACAGCCGCAGTTGCAGAGCTCCTCGCT 120


1815R-3.IR_full       121 GGATGGCCACGTCGAGATGGAGCCGGTCATCATGCCCAGCAAGCGTCAAAAGTATATTGT 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1815R-3.in silico     121 GGATGGCCACGTCGAGATGGAGCCGGTCATCATGCCCAGCAAGCGTCAAAAGTATATTGT 180


1815R-3.IR_full       181 CAGTGCTCCGCCNTGGGTCGGCCAACTGCAGCAGTAACTCATCCAGTTCGAGTCCCTCGA 240
                          |||||||||||| |||||||||||||||||||||||||||||||||||||||||||||||
1815R-3.in silico     181 CAGTGCTCCGCC-TGGGTCGGCCAACTGCAGCAGTAACTCATCCAGTTCGAGTCCCTCGA 240


1815R-3.IR_full       241 ACATCAACGTGGGCTCCTCGCTTACCATGAAGTTGACGAAGGTGCAGCCACAACAGCATC 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1815R-3.in silico     241 ACATCAACGTGGGCTCCTCGCTTACCATGAAGTTGACGAAGGTGCAGCCACAACAGCATC 300


1815R-3.IR_full       301 AGGCTAAGTCCTCCACGCCAAACAACAAAGGCAAAGTAGCCAAGGAAATGGTTGCTCTGC 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1815R-3.in silico     301 AGGCTAAGTCCTCCACGCCAAACAACAAAGGCAAAGTAGCCAAGGAAATGGTTGCTCTGC 360


1815R-3.IR_full       361 AGCGCTCACACATCGAATCCGAGGTCCTCAGCAACTTTGTGACTGGGGTGTCCAAGCTGA 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1815R-3.in silico     361 AGCGCTCACACATCGAATCCGAGGTCCTCAGCAACTTTGTGACTGGGGTGTCCAAGCTGA 420


1815R-3.IR_full       421 AGAGTCGAAAGTCGCGATATGTGCCCGGGAATCTCGCCACTCAGGCAGTAGGTGGAGAGC 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1815R-3.in silico     421 AGAGTCGAAAGTCGCGATATGTGCCCGGGAATCTCGCCACTCAGGCAGTAGGTGGAGAGC 480


1815R-3.IR_full       481 TGTCCCGCAGCTTAAATACCTC 502
                          ||||||||||||||||||||||
1815R-3.in silico     481 TGTCCCGCAGCTTAAATACCTC 502



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_170562.1  CG1815-RA, transcript variant A (CG1815), mRNA 
100   482  0  0  0  NM_143624.2  CG1815-RC, transcript variant C (CG1815), mRNA 
100   482  0  0  0  NM_170563.1  CG1815-RB, transcript variant B (CG1815), mRNA 
0   0  4  3  3  NM_141220.1  CG9783-RA (CG9783), mRNA 
0   0  3  3  4  NM_080488.2  CG6349-RA (DNApol-alpha180), mRNA 
0   0  2  5  15  NM_001031889.1  CG33691-RA, transcript variant A (CG33691), mRNA 
0   0  2  5  15  NM_001031886.1  CG33692-RA, transcript variant A (CG33692), mRNA 
0   0  2  5  10  NM_001031887.1  CG33691-RC, transcript variant C (CG33691), mRNA 
0   0  2  3  4  NM_142062.1  CG14363-RA (CG14363), mRNA 
0   0  2  2  3  NM_079898.2  CG11081-RA, transcript variant A (plexA), mRNA 
0   0  2  2  3  NM_166806.2  CG11081-RD, transcript variant D (plexA), mRNA 
0   0  2  2  2  NM_001038715.1  CG11081-RE, transcript variant E (plexA), mRNA 
0   0  1  9  53  NM_135077.2  CG14023-RA (CG14023), mRNA 
0   0  1  6  15  NM_078575.2  CG9355-RA (dy), mRNA 
0   0  1  5  12  NM_137196.1  CG12964-RA (CG12964), mRNA 
0   0  1  5  12  NM_139766.1  CG6619-RA (CG6619), mRNA 
0   0  1  4  17  NM_136154.1  CG10631-RA (CG10631), mRNA 
0   0  1  4  9  NM_135954.1  CG13260-RA (CG13260), mRNA 
0   0  1  4  4  NM_137617.3  CG11209-RA (ppk6), mRNA 
0   0  1  4  3  NM_142862.1  CG17111-RA (CG17111), mRNA 
0   0  1  4  3  NM_166440.1  CG30386-RA (CG30386), mRNA 
0   0  1  4  3  NM_167505.1  CG4211-RB, transcript variant B (nonA), mRNA 
0   0  1  4  3  NM_001014747.1  CG4211-RC, transcript variant C (nonA), mRNA 
0   0  1  4  3  NM_078643.2  CG4211-RA, transcript variant A (nonA), mRNA 
0   0  1  3  15  NM_137564.2  CG7417-RA (Tab2), mRNA 
0   0  1  3  10  NM_166331.1  CG15112-RB, transcript variant B (ena), mRNA 
0   0  1  3  10  NM_001014537.1  CG15112-RD, transcript variant D (ena), mRNA 
0   0  1  3  10  NM_166329.1  CG15112-RA, transcript variant A (ena), mRNA 
0   0  1  3  10  NM_001014536.1  CG15112-RE, transcript variant E (ena), mRNA 
0   0  1  3  10  NM_166330.1  CG15112-RC, transcript variant C (ena), mRNA 
 Wing Disc
GFP
 Puc
 Caspase
DAPI
 Merge
 Figure
JNK signal
N
 
DB
 
DC
+ 
DM
 
VM
 
VB
 
Caspase-3 signal
N
 
DB
 
DC
 
DM
 
VM
 
VB
 
Dcompartment   Penetrance
+     

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