NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 18155R-1

Symbol CG18155 Full Name CG18155

CG No CG18155 Old CG No CG18155

Synonyms FBgn0029945, CG18155

Accession No (Link to NCBI) NM_132164.1

Inserted Chr. lll

Insertional Mutation lethal

Phenotype induced by Act5C-GAL4 at 28 degrees viable

Map Viewer

Reference
[Please submit your publication] Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624 [ PubMed ID = 21666802 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCTGGTGGCGATCCAGGGTGTT

Primer Seq. 3'

aatctagaggtaccACCGAATCCGGTAGCAAGGCA

Predicted Fragment Size

IR fragment full Seq

0001 CTGGTGGCGA TCCAGGGTGT TATCGTAACG GAAAATACGT TTCCAAACGA TTTCTATTCC 
0061 AAGGCACCAG CCATGCTGAT CTATACGCCC AATGCGGTCA ATAGTCCCAA GCCAGTGTTG 
0121 TTGACCCATC GGAATATCGA AGCTCAGATG CGATGTTTGA TCGGAACTTG GCATTTGGGA 
0181 CCTACAGACT GTATGCTGCC AATACTATCC ATGAATCGAA TGCATGCCGC CTTGGCAGCA 
0241 GTTCTCAGTG TGGGCGGTAA TGTGGTGTTG CAGCAAAAAT TCGATGGTCA CAATGCGTGG 
0301 AGTGCTCTAT TGGGCATAAA TAGCCCATCC AAGCAACGGG TCACCCTCTT TTTGGCCATG 
0361 CCAATTTGTC TACAAGCGTC TGATTGTCGA GTATGAGAAA ATGTTTGCCA AGGATTCGAG 
0421 AATGGTTGAG TACATTGTGA ATCATTGCCG GCAAAAGATA CGATTGATGG CGACAGCTTT 
0481 TGCCTTGCTA CCGGATTCGG T

in silico PCR Fragment

0001 CTGGTGGCGA TCCAGGGTGT TATCGTAACG GAAAATACGT TTCCAAACGA TTTCTATTCC 
0061 AAGGCACCAG CCATGCTGAT CTATACGCCC AATGCGGTCA ATAGTCCCAA GCCAGTGTTG 
0121 TTGACCCATC GGAATATCGA AGCTCAGATG CGATGTTTGA TCGGAACTTG GCATTTGGGA 
0181 CCTACAGACT GTATGCTGCC AATACTATCC ATGAATCGAA TGCATGCCGC CTTGGCAGCA 
0241 GTTCTCAGTG TGGGCGGTAA TGTGGTGTTG CAGCAAAAAT TCGATGGTCA CAATGCGTGG 
0301 AGTGCTCTAT TGGGCATAAA TAGCCCATCC AAGCAACGGG TCACCCTCTT TTTGGCCATG 
0361 CCAA-TTGTC TACAAGCGTC TGATTGTCGA GTATGAGAAA ATGTTTGCCA AGGATTCGAG 
0421 AATGGTTGAG TACATTGTGA ATCATTGCCG GCAAAAGATA CGATTGATGG CGACAGCTTT 
0481 TGCCTTGCTA CCGGATTCGG T

Assemble Data


18155R-1.IR_full       1   CTGGTGGCGATCCAGGGTGTTATCGTAACGGAAAATACGTTTCCAAACGATTTCTATTCC 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18155R-1.in silico     1   CTGGTGGCGATCCAGGGTGTTATCGTAACGGAAAATACGTTTCCAAACGATTTCTATTCC 60


18155R-1.IR_full       61  AAGGCACCAGCCATGCTGATCTATACGCCCAATGCGGTCAATAGTCCCAAGCCAGTGTTG 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18155R-1.in silico     61  AAGGCACCAGCCATGCTGATCTATACGCCCAATGCGGTCAATAGTCCCAAGCCAGTGTTG 120


18155R-1.IR_full       121 TTGACCCATCGGAATATCGAAGCTCAGATGCGATGTTTGATCGGAACTTGGCATTTGGGA 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18155R-1.in silico     121 TTGACCCATCGGAATATCGAAGCTCAGATGCGATGTTTGATCGGAACTTGGCATTTGGGA 180


18155R-1.IR_full       181 CCTACAGACTGTATGCTGCCAATACTATCCATGAATCGAATGCATGCCGCCTTGGCAGCA 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18155R-1.in silico     181 CCTACAGACTGTATGCTGCCAATACTATCCATGAATCGAATGCATGCCGCCTTGGCAGCA 240


18155R-1.IR_full       241 GTTCTCAGTGTGGGCGGTAATGTGGTGTTGCAGCAAAAATTCGATGGTCACAATGCGTGG 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18155R-1.in silico     241 GTTCTCAGTGTGGGCGGTAATGTGGTGTTGCAGCAAAAATTCGATGGTCACAATGCGTGG 300


18155R-1.IR_full       301 AGTGCTCTATTGGGCATAAATAGCCCATCCAAGCAACGGGTCACCCTCTTTTTGGCCATG 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18155R-1.in silico     301 AGTGCTCTATTGGGCATAAATAGCCCATCCAAGCAACGGGTCACCCTCTTTTTGGCCATG 360


18155R-1.IR_full       361 CCAATTTGTCTACAAGCGTCTGATTGTCGAGTATGAGAAAATGTTTGCCAAGGATTCGAG 420
                           |||| |||||||||||||||||||||||||||||||||||||||||||||||||||||||
18155R-1.in silico     361 CCAA-TTGTCTACAAGCGTCTGATTGTCGAGTATGAGAAAATGTTTGCCAAGGATTCGAG 420


18155R-1.IR_full       421 AATGGTTGAGTACATTGTGAATCATTGCCGGCAAAAGATACGATTGATGGCGACAGCTTT 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18155R-1.in silico     421 AATGGTTGAGTACATTGTGAATCATTGCCGGCAAAAGATACGATTGATGGCGACAGCTTT 480


18155R-1.IR_full       481 TGCCTTGCTACCGGATTCGGT 501
                           |||||||||||||||||||||
18155R-1.in silico     481 TGCCTTGCTACCGGATTCGGT 501

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_206637.1	CG18155-RB, transcript variant B (CG18155), mRNA
100	482	0	0	0	NM_132164.1	CG18155-RA, transcript variant A (CG18155), mRNA
0	0	2	2	2	NM_139695.1	CG4597-RA (CG4597), mRNA
0	0	1	3	5	NM_166030.1	CG8118-RB, transcript variant B (mam), mRNA
0	0	1	3	5	NM_080376.1	CG8118-RA, transcript variant A (mam), mRNA
0	0	1	3	3	NM_001014545.1	CG33519-RB (Unc-89), mRNA
0	0	1	2	5	NM_078694.2	CG17058-RA, transcript variant A (Peritrophin-A), mRNA
0	0	1	2	5	NM_167694.1	CG17058-RB, transcript variant B (Peritrophin-A), mRNA
0	0	1	2	4	NM_175983.1	CG8222-RC, transcript variant C (Pvr), mRNA
0	0	1	2	4	NM_205926.1	CG8222-RE, transcript variant E (Pvr), mRNA
0	0	1	2	4	NM_205925.1	CG8222-RF, transcript variant F (Pvr), mRNA
0	0	1	2	4	NM_078785.2	CG8222-RA, transcript variant A (Pvr), mRNA
0	0	1	2	4	NM_164801.1	CG8222-RB, transcript variant B (Pvr), mRNA
0	0	1	2	4	NM_205927.1	CG8222-RD, transcript variant D (Pvr), mRNA
0	0	1	2	3	NM_176408.1	CG33097-RA, transcript variant A (CG33097), mRNA
0	0	1	2	3	NM_001043218.1	CG33097-RB, transcript variant B (CG33097), mRNA
0	0	1	2	2	NM_080341.2	CG12737-RA, transcript variant A (Crag), mRNA
0	0	1	2	2	NM_167164.2	CG12737-RB, transcript variant B (Crag), mRNA
0	0	1	1	1	NM_130543.3	CG32812-RA (CG32812), mRNA
0	0	0	5	5	NM_136041.1	CG10231-RA (Pde11), mRNA
0	0	0	4	4	NM_001014604.1	CG9795-RD, transcript variant D (CG9795), mRNA
0	0	0	4	4	NM_141197.3	CG9795-RA, transcript variant A (CG9795), mRNA
0	0	0	4	4	NM_176396.1	CG9795-RC, transcript variant C (CG9795), mRNA
0	0	0	4	4	NM_164338.2	CG9795-RB, transcript variant B (CG9795), mRNA
0	0	0	4	2	NM_205898.1	CG33281-RA (CG33281), mRNA
0	0	0	4	2	NM_078656.3	CG9108-RA (RSG7), mRNA
0	0	0	4	2	NM_206430.1	CG2666-RC, transcript variant C (kkv), mRNA
0	0	0	4	2	NM_079509.1	CG2666-RA, transcript variant A (kkv), mRNA
0	0	0	4	2	NM_169052.1	CG2666-RB, transcript variant B (kkv), mRNA
0	0	0	3	8	NM_135506.1	CG13137-RA (CG13137), mRNA

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