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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 18110R-1 
 Symbol CG18110  Full Name CG18110 
 CG No CG18110  Old CG No CG18110 
 Synonyms CG18110 
 Accession No (Link to NCBI) NM_143449.1 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees pupal semi-lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]

Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgCTTCTACTACTGGGTGCAGAC 
 Primer Seq. 3' 
aatctagaggtaccGCACTATCACCCCCATTGTAT 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 CTTCTACTAC TGGGTGCAGA CCTCCATCGA AAGGACTGAC ATGCATGTAT CCGAGATCGA 
0061 TTTCCCAGCC GTGACCATCA TTCCGATCAA TTTGAGCATC CTGAAAGCCG AGAAGCTCTC 
0121 CAAGGCCTAT AACTTGGTTC AGTCCGTGGT CTGGCAAACA CCGATGTCCG CCCGCCTCAC 
0181 GGATGAGAAC TTCACGGAGT TCTCCGAACT GGAAAACTGG AACGGCGCAG AGCTGGGGAA 
0241 TCTACCAGGC GCTGCAGATG AACTGCCAGC ACTTCTTCAC CGAGTGCCAG TGGCGTCGCA 
0301 AGGCGATGAA CTGCTGTGAC CTATTCCGAC CCACCAAGAC CTTCAATGGC TTCGCCTTCG 
0361 AGTTCAACTC ACTGGTCTCC TCGGGAAGAG ATGAAACGTG GCCCTGGTCT GTGGCTTCAT 
0421 GCGGATCATA TAGCGGACTC AATGTGAAGA TCAAACGGCA GCAGGGTCTG TATACGTTGA 
0481 ATACAATGGG GGTGATAgTG N 
 in silico PCR Fragment 
0001 CTTCTACTAC TGGGTGCAGA CCTCCATCGA AAGGACTGAC ATGCATGTAT CCGAGATCGA 
0061 TTTCCCAGCC GTGACCATCA TTCCGATCAA TTTGAGCATC CTGAAAGCCG AGAAGCTCTC 
0121 CAAGGCCTAT AACTTGGTTC AGTCCGTGGT CTGGCAAACA CCGATGTCCG CCCGCCTCAC 
0181 GGATGAGAAC TTCACGGAGT TCTCCGAACT GGAAAACTGG AAC-GCGCAG AGCTGGGGAA 
0241 TCTACCAGGC GCTGCAGATG AACTGCCAGC ACTTCTTCAC CGAGTGCCAG TGGCGTCGCA 
0301 AGGCGATGAA CTGCTGTGAC CTATTCCGAC CCACCAAGAC CTTCAATGGC TTCGCCTTCG 
0361 AGTTCAACTC ACTGGTCTCC TCGGGAAGAG ATGAAACGTG GCCCTGGTCC GTGGCTTCAT 
0421 GCGGATCATA TAGCGGACTC AATGTGAAGA TCAAACGGCA GCAGGGTCTG TATACGTTGA 
0481 ATACAATGGG GGTGATAGTG C 
 Assemble Data 

18110R-1.IR_full       1   CTTCTACTACTGGGTGCAGACCTCCATCGAAAGGACTGACATGCATGTATCCGAGATCGA 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18110R-1.in silico     1   CTTCTACTACTGGGTGCAGACCTCCATCGAAAGGACTGACATGCATGTATCCGAGATCGA 60


18110R-1.IR_full       61  TTTCCCAGCCGTGACCATCATTCCGATCAATTTGAGCATCCTGAAAGCCGAGAAGCTCTC 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18110R-1.in silico     61  TTTCCCAGCCGTGACCATCATTCCGATCAATTTGAGCATCCTGAAAGCCGAGAAGCTCTC 120


18110R-1.IR_full       121 CAAGGCCTATAACTTGGTTCAGTCCGTGGTCTGGCAAACACCGATGTCCGCCCGCCTCAC 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18110R-1.in silico     121 CAAGGCCTATAACTTGGTTCAGTCCGTGGTCTGGCAAACACCGATGTCCGCCCGCCTCAC 180


18110R-1.IR_full       181 GGATGAGAACTTCACGGAGTTCTCCGAACTGGAAAACTGGAACGGCGCAGAGCTGGGGAA 240
                           ||||||||||||||||||||||||||||||||||||||||||| ||||||||||||||||
18110R-1.in silico     181 GGATGAGAACTTCACGGAGTTCTCCGAACTGGAAAACTGGAAC-GCGCAGAGCTGGGGAA 240


18110R-1.IR_full       241 TCTACCAGGCGCTGCAGATGAACTGCCAGCACTTCTTCACCGAGTGCCAGTGGCGTCGCA 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18110R-1.in silico     241 TCTACCAGGCGCTGCAGATGAACTGCCAGCACTTCTTCACCGAGTGCCAGTGGCGTCGCA 300


18110R-1.IR_full       301 AGGCGATGAACTGCTGTGACCTATTCCGACCCACCAAGACCTTCAATGGCTTCGCCTTCG 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18110R-1.in silico     301 AGGCGATGAACTGCTGTGACCTATTCCGACCCACCAAGACCTTCAATGGCTTCGCCTTCG 360


18110R-1.IR_full       361 AGTTCAACTCACTGGTCTCCTCGGGAAGAGATGAAACGTGGCCCTGGTCTGTGGCTTCAT 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||| ||||||||||
18110R-1.in silico     361 AGTTCAACTCACTGGTCTCCTCGGGAAGAGATGAAACGTGGCCCTGGTCCGTGGCTTCAT 420


18110R-1.IR_full       421 GCGGATCATATAGCGGACTCAATGTGAAGATCAAACGGCAGCAGGGTCTGTATACGTTGA 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18110R-1.in silico     421 GCGGATCATATAGCGGACTCAATGTGAAGATCAAACGGCAGCAGGGTCTGTATACGTTGA 480


18110R-1.IR_full       481 ATACAATGGGGGTGATAGTGN 501
                           |||||||||||||||||||| 
18110R-1.in silico     481 ATACAATGGGGGTGATAGTGC 501



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_143449.1  CG18110-RA (CG18110), mRNA 
0   0  3  3  4  NM_140563.1  CG17026-RA (CG17026), mRNA 
0   0  2  4  2  NM_140713.1  CG16793-RA (CG16793), mRNA 
0   0  2  3  3  NM_135400.1  CG9287-RA (CG9287), mRNA 
0   0  1  4  3  NM_137278.1  CG15710-RA (CG15710), mRNA 
0   0  1  2  4  NM_001043133.1  CG32045-RD, transcript variant D (fry), mRNA 
0   0  1  2  4  NM_168357.2  CG32045-RB, transcript variant B (fry), mRNA 
0   0  0  5  3  NM_079494.3  CG8779-RA, transcript variant A (nrm), mRNA 
0   0  0  5  3  NM_001043156.1  CG8779-RB, transcript variant B (nrm), mRNA 
0   0  0  5  2  NM_168514.1  CG32106-RA (CG32106), mRNA 
0   0  0  4  25  NM_167559.1  CG32564-RA (CG32564), mRNA 
0   0  0  4  7  NM_166129.2  CG18255-RD, transcript variant D (Strn-Mlck), mRNA 
0   0  0  4  7  NM_166125.2  CG18255-RA, transcript variant A (Strn-Mlck), mRNA 
0   0  0  4  4  NM_205940.1  CG10595-RB, transcript variant B (d), mRNA 
0   0  0  4  4  NM_205939.1  CG10595-RC, transcript variant C (d), mRNA 
0   0  0  4  4  NM_175991.1  CG10595-RA, transcript variant A (d), mRNA 
0   0  0  4  3  NR_001615.1  CR31969, miscRNA 
0   0  0  4  2  NM_169201.1  CG31284-RB, transcript variant B (CG31284), mRNA 
0   0  0  4  2  NM_206459.1  CG31284-RC, transcript variant C (CG31284), mRNA 
0   0  0  4  2  NM_169200.1  CG31284-RA, transcript variant A (CG31284), mRNA 
0   0  0  4  2  NM_132214.1  CG15335-RB (CG15335), mRNA 
0   0  0  4  2  NM_170085.2  CG31414-RA (CG31414), mRNA 
0   0  0  4  2  NM_140263.1  CG14127-RA (CG14127), mRNA 
0   0  0  4  2  NM_132189.1  CG1422-RA (p115), mRNA 
0   0  0  3  8  NM_141779.2  CG6621-RA (CG6621), mRNA 
0   0  0  3  7  NM_132349.2  CG3106-RA (CG3106), mRNA 
0   0  0  3  6  NM_135979.1  CG15134-RA (CG15134), mRNA 
0   0  0  3  6  NM_079939.2  CG5580-RA, transcript variant A (sbb), mRNA 
0   0  0  3  6  NM_165666.1  CG2040-RB, transcript variant B (hig), mRNA 
0   0  0  3  6  NM_167940.1  CG32305-RA (CG32305), mRNA 
 Wing Disc
GFP
 Puc
 Caspase
DAPI
 Merge
 Figure
JNK signal
N
 
DB
 
DC
+- 
DM
 
VM
 
VB
 
Caspase-3 signal
N
+ 
DB
+ 
DC
+ 
DM
+ 
VM
 
VB
 
Dcompartment   Penetrance
     

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