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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 18024R-2 
 Symbol SoxN  Full Name SoxNeuro 
 CG No CG18024  Old CG No CG18024 
 Synonyms soxN, Sox B1, CG18024, SOXB1, SOX29F, DM64, Sry-rDM64, SoxN 
 Accession No (Link to NCBI) NM_079996.2 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Nitta Y, Yamazaki D, Sugie A, Hiroi M, Tabata T.
DISCO Interacting Protein 2 regulates axonal bifurcation and guidance of Drosophila mushroom body neurons.
Dev Biol (2017) 421(2) 233-244 [ PubMed ID = 27908785 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgTGCTGACCATGGAATCGGATA 
 Primer Seq. 3' 
aatctagaggtaccGGCGCTGTTGTTGTTGTTGTT 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 TGCTGACCAT GGAATCGGAT ATGAAAGGCA GCCTGCTGCA TGCCACCATG CCGCCGCATC 
0061 ACACAAGTGC CGCGTTGCAC GGGACATGCG GCATCGCCGT ACTCCGCCCT AGCTCCCCTT 
0121 ATGAACCTGG GCCAGTCGCA CCTGACCCAC TCGCAGCTGA GCCATCACAA CCACCATCAT 
0181 CACCACATGA GCGCCCATAT CGCGGCCAGC CAGAGCCCCA ATCCGCTGAG CTCGCTCCAG 
0241 TCCTCGATGG CGAACACCCT CAATGGCAGC CAGGTGGGGC AGCAGCAGCA GCAACAGCAG 
0301 CAGCAGCAGA GNTCCNCCGC TGCACAGCTC CAGCGAATTG AGTCCCACCC AGAGCAGCAT 
0361 CGGAAGCCAT CACATGACAT CGCCGGTCAG CCACCAGCAG CACACGCAAC AGCAGCAGCA 
0421 CGGGCAGCAG CAGCATCTGG GCGCGGGATT CTGCATTGAG CAGCCNTAAC TGGAGGATCC 
0481 AGCAACAACA ACAACAANCA GCGCc 
 in silico PCR Fragment 
0001 TGCTGACCAT GGAATCGGAT ATGAAAGGCA GCCTGCTGCA TGCCACCATG CCGCCGCATC 
0061 ACACAAGTGC CGCGTTGCAC -GGACATGCG GCATCGCCGT ACTCCGCCCT AGCTCCCCTT 
0121 ATGAACCTGG GCCAGTCGCA CCTGACCCAC TCGCAGCTGA GCCATCACAA CCACCATCAT 
0181 CACCACATGA GCGCCCATAT CGCGGCCAGC CAGAGTCCCA ATCCGCTGAG CTCGCTGCAG 
0241 TCCTCGATGG CGAACACCCT CAATGGCAGC CAGGTGGGGC AGCAGCAGCA GCAACAGCAG 
0301 CAGCAGCAGA GCTCC-CCGC TGCACAGCTC CAGCGAATTG AGTCCCACCC AGAGCAGCAT 
0361 CGGAAGCCAT CACATGACAT CGCCGGTCAG CCACCAGCAG CACACGCAAC AGCAGCAGCA 
0421 CGGGCAGCAG CAGCATCTGG GCGCGGGA-T CTGCATTGAG CAGCC-TAAC TGGAGGATCC 
0481 AGCAACAACA ACAACAA-CA GCGCC 
 Assemble Data 

18024R-2.IR_full       1   TGCTGACCATGGAATCGGATATGAAAGGCAGCCTGCTGCATGCCACCATGCCGCCGCATC 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18024R-2.in silico     1   TGCTGACCATGGAATCGGATATGAAAGGCAGCCTGCTGCATGCCACCATGCCGCCGCATC 60


18024R-2.IR_full       61  ACACAAGTGCCGCGTTGCACGGGACATGCGGCATCGCCGTACTCCGCCCTAGCTCCCCTT 120
                           |||||||||||||||||||| |||||||||||||||||||||||||||||||||||||||
18024R-2.in silico     61  ACACAAGTGCCGCGTTGCAC-GGACATGCGGCATCGCCGTACTCCGCCCTAGCTCCCCTT 120


18024R-2.IR_full       121 ATGAACCTGGGCCAGTCGCACCTGACCCACTCGCAGCTGAGCCATCACAACCACCATCAT 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18024R-2.in silico     121 ATGAACCTGGGCCAGTCGCACCTGACCCACTCGCAGCTGAGCCATCACAACCACCATCAT 180


18024R-2.IR_full       181 CACCACATGAGCGCCCATATCGCGGCCAGCCAGAGCCCCAATCCGCTGAGCTCGCTCCAG 240
                           ||||||||||||||||||||||||||||||||||| |||||||||||||||||||| |||
18024R-2.in silico     181 CACCACATGAGCGCCCATATCGCGGCCAGCCAGAGTCCCAATCCGCTGAGCTCGCTGCAG 240


18024R-2.IR_full       241 TCCTCGATGGCGAACACCCTCAATGGCAGCCAGGTGGGGCAGCAGCAGCAGCAACAGCAG 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18024R-2.in silico     241 TCCTCGATGGCGAACACCCTCAATGGCAGCCAGGTGGGGCAGCAGCAGCAGCAACAGCAG 300


18024R-2.IR_full       301 CAGCAGCAGAGNTCCNCCGCTGCACAGCTCCAGCGAATTGAGTCCCACCCAGAGCAGCAT 360
                           ||||||||||| ||| ||||||||||||||||||||||||||||||||||||||||||||
18024R-2.in silico     301 CAGCAGCAGAGCTCC-CCGCTGCACAGCTCCAGCGAATTGAGTCCCACCCAGAGCAGCAT 360


18024R-2.IR_full       361 CGGAAGCCATCACATGACATCGCCGGTCAGCCACCAGCAGCACACGCAACAGCAGCAGCA 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
18024R-2.in silico     361 CGGAAGCCATCACATGACATCGCCGGTCAGCCACCAGCAGCACACGCAACAGCAGCAGCA 420


18024R-2.IR_full       421 CGGGCAGCAGCAGCATCTGGGCGCGGGATTCTGCATTGAGCAGCCNTAACTGGAGGATCC 480
                           |||||||||||||||||||||||||||| |||||||||||||||| ||||||||||||||
18024R-2.in silico     421 CGGGCAGCAGCAGCATCTGGGCGCGGGA-TCTGCATTGAGCAGCC-TAACTGGAGGATCC 480


18024R-2.IR_full       481 AGCAACAACAACAACAANCAGCGCC 505
                           ||||||||||||||||| |||||||
18024R-2.in silico     481 AGCAACAACAACAACAA-CAGCGCC 505



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
102.28  493  52  235  462  NM_079996.2  CG18024-RA (SoxN), mRNA 
16.39   79  693  1190  2373  NM_167335.1  CG4013-RC, transcript variant C (Smr), mRNA 
16.39   79  693  1190  2373  NM_080536.2  CG4013-RA, transcript variant A (Smr), mRNA 
16.39   79  693  1190  2373  NM_167334.1  CG4013-RB, transcript variant B (Smr), mRNA 
6.63   32  59  390  593  NM_080364.3  CG5461-RA, transcript variant A (bun), mRNA 
6.63   32  53  356  506  NM_001042894.1  CG5461-RF, transcript variant F (bun), mRNA 
6.43   31  89  456  791  NM_139493.2  CG2083-RA (CG2083), mRNA 
6.43   31  50  238  427  NM_132004.2  CG4136-RA (CG4136), mRNA 
6.22   30  166  412  877  NM_079671.2  CG7847-RA, transcript variant A (sr), mRNA 
6.01   29  232  541  1183  NM_080376.1  CG8118-RA, transcript variant A (mam), mRNA 
6.01   29  232  541  1183  NM_166030.1  CG8118-RB, transcript variant B (mam), mRNA 
6.01   29  87  319  490  NM_167000.1  CG32778-RA (CG32778), mRNA 
5.6   27  89  209  555  NM_169386.1  CG17228-RA, transcript variant A (pros), mRNA 
5.6   27  73  159  350  NM_166446.1  CG9696-RD, transcript variant D (dom), mRNA 
5.6   27  73  159  350  NM_080094.2  CG9696-RA, transcript variant A (dom), mRNA 
5.6   27  38  134  240  NM_057511.3  CG3936-RA (N), mRNA 
5.39   26  148  332  749  NM_206628.1  CG32767-RB, transcript variant B (CG32767), mRNA 
5.39   26  148  332  749  NM_131975.3  CG32767-RA, transcript variant A (CG32767), mRNA 
5.39   26  129  416  803  NM_079903.2  CG15319-RB (nej), mRNA 
5.39   26  64  289  337  NM_206456.1  CG32466-RB, transcript variant B (rn), mRNA 
4.97   24  73  245  512  NM_079507.2  CG2530-RA (corto), mRNA 
4.77   23  148  357  753  NM_169786.1  CG7847-RB, transcript variant B (sr), mRNA 
4.77   23  94  292  516  NM_143575.2  CG12071-RA, transcript variant A (CG12071), mRNA 
4.77   23  94  292  516  NM_176592.1  CG12071-RB, transcript variant B (CG12071), mRNA 
4.77   23  51  220  282  NM_143700.3  CG17724-RA, transcript variant A (CG17724), mRNA 
4.56   22  164  558  1066  NM_001038734.1  CG16902-RC (Hr4), mRNA 
4.56   22  70  222  527  NM_078592.2  CG11172-RA (NFAT), mRNA 
4.56   22  61  260  667  NM_132403.3  CG17255-RA, transcript variant A (CG17255), mRNA 
4.56   22  61  260  667  NM_167231.3  CG17255-RB, transcript variant B (CG17255), mRNA 
4.56   22  28  168  369  NM_176542.1  CG7029-RA (CG7029), mRNA 

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