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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 17999R-1 
 Symbol CG17999  Full Name CG17999 
 CG No CG17999  Old CG No CG17999 
 Synonyms CG17999 
 Accession No (Link to NCBI) NM_137673.2 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication]
Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5'
aaggcctacatggccggaccgCCAGAAATGACCCTGGGCGAG 
 Primer Seq. 3'
aatctagaggtaccGCGGTGTGATCGCCGTGTAGT 
 Predicted Fragment Size
506 
 IR fragment full Seq
0001 CCAGAAATGA CCCTGGGCGA GGTCATCATG CGCGTGCTGC AGATTAACGC AGATCAGGTG 
0061 ATGCAAATAT GTGATACCAC GGGTCAGGAG CTCACCGGTG CTCAGTTGGC TCAGCAGAGT 
0121 GCCCGCATAG CACAGGCCTT CAAGAGATTG GGTCTACGGA GGGGCGATGT CGTTGGCATA 
0181 TCGGCCAATA ACTCGACGTA TTTAACCAGT GTGATAATTG CGGCTTTGCT CCGTGGCATC 
0241 CCCATTAATC CACTGCATCC GGAGTTTACT GAAGAGACTG TCAAATACAT GTACGACATT 
0301 ACCGAGCCGA AGGTTATCTT CTGCGATGTA GAAAACTACC ATATAATAAA GACGGTCAAT 
0361 GGAAAACTAC AAAATCCCGC CAAGATCTAT CTGGTCAATG GAAAATTAGA AGGAGTGCTG 
0421 GACATAAGCG AGATGCTAAA TGATGAAGAC AGCATTACAG CAGCCGCGTA TGTTCCTTGT 
0481 CCAAAACTAC ACGGCGATCA CACCGC 
 in silico PCR Fragment
0001 CCAGAAATGA CCCTGGGCGA GGTCATCATG CGCGTGCTGC AGATTAACGC AGATCAGGTG 
0061 ATGCAAATAT GTGATACCAC GGGTCAGGAG CTCACCGGTG CTCAGTTGGC TCAGCAGAGT 
0121 GCCCGCATAG CACAGGCCTT CAAGAGATTG GGTCTACGGA GGGGCGATGT CGTTGGCATA 
0181 TCGGCCAATA ACTCGACGTA TTTAACCAGT GTGATAATTG CGGCTTTGCT CCGTGGCATC 
0241 CCCATTAATC CACTGCATCC GGAGTTTACT GAAGAGACTG TCAAATACAT GTACGACATT 
0301 ACCGAGCCGA AGGTTATCTT CTGCGATGTA GAAAACTACC ATATAATAAA GACGGTCAAT 
0361 GGAAAACTAC AAAATCCCGC CAAGATCTAT CTGGTCAATG GAAAATTAGA AGGAGTGCTG 
0421 GACATAAGCG AGATGCTAAA TGATGAAGAC AGCATTACAG CAGCCGCGTA TGTTCCTTGT 
0481 CCAAAACTAC ACGGCGATCA CACCGC 
 Assemble Data

17999R-1.IR_full       1   CCAGAAATGACCCTGGGCGAGGTCATCATGCGCGTGCTGCAGATTAACGCAGATCAGGTG 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17999R-1.in silico     1   CCAGAAATGACCCTGGGCGAGGTCATCATGCGCGTGCTGCAGATTAACGCAGATCAGGTG 60


17999R-1.IR_full       61  ATGCAAATATGTGATACCACGGGTCAGGAGCTCACCGGTGCTCAGTTGGCTCAGCAGAGT 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17999R-1.in silico     61  ATGCAAATATGTGATACCACGGGTCAGGAGCTCACCGGTGCTCAGTTGGCTCAGCAGAGT 120


17999R-1.IR_full       121 GCCCGCATAGCACAGGCCTTCAAGAGATTGGGTCTACGGAGGGGCGATGTCGTTGGCATA 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17999R-1.in silico     121 GCCCGCATAGCACAGGCCTTCAAGAGATTGGGTCTACGGAGGGGCGATGTCGTTGGCATA 180


17999R-1.IR_full       181 TCGGCCAATAACTCGACGTATTTAACCAGTGTGATAATTGCGGCTTTGCTCCGTGGCATC 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17999R-1.in silico     181 TCGGCCAATAACTCGACGTATTTAACCAGTGTGATAATTGCGGCTTTGCTCCGTGGCATC 240


17999R-1.IR_full       241 CCCATTAATCCACTGCATCCGGAGTTTACTGAAGAGACTGTCAAATACATGTACGACATT 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17999R-1.in silico     241 CCCATTAATCCACTGCATCCGGAGTTTACTGAAGAGACTGTCAAATACATGTACGACATT 300


17999R-1.IR_full       301 ACCGAGCCGAAGGTTATCTTCTGCGATGTAGAAAACTACCATATAATAAAGACGGTCAAT 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17999R-1.in silico     301 ACCGAGCCGAAGGTTATCTTCTGCGATGTAGAAAACTACCATATAATAAAGACGGTCAAT 360


17999R-1.IR_full       361 GGAAAACTACAAAATCCCGCCAAGATCTATCTGGTCAATGGAAAATTAGAAGGAGTGCTG 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17999R-1.in silico     361 GGAAAACTACAAAATCCCGCCAAGATCTATCTGGTCAATGGAAAATTAGAAGGAGTGCTG 420


17999R-1.IR_full       421 GACATAAGCGAGATGCTAAATGATGAAGACAGCATTACAGCAGCCGCGTATGTTCCTTGT 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17999R-1.in silico     421 GACATAAGCGAGATGCTAAATGATGAAGACAGCATTACAGCAGCCGCGTATGTTCCTTGT 480


17999R-1.IR_full       481 CCAAAACTACACGGCGATCACACCGC 506
                           ||||||||||||||||||||||||||
17999R-1.in silico     481 CCAAAACTACACGGCGATCACACCGC 506

 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   488  NM_137673.2  CG17999-RA (CG17999), mRNA 
0   NM_132908.2  CG4453-RA (Nup153), mRNA 
0   NM_078496.2  CG3025-RA (mof), mRNA 
0   NM_136068.2  CG17597-RA (CG17597), mRNA 
0   31  26  NM_137674.1  CG9993-RA (CG9993), mRNA 
0   NM_138050.2  CG3363-RA (CG3363), mRNA 
0   NM_136828.1  CG13209-RA (sha), mRNA 
0   NM_142745.2  CG6028-RA, transcript variant A (CG6028), mRNA 
0   NM_169989.1  CG6028-RB, transcript variant B (CG6028), mRNA 
0   NM_079336.2  CG7002-RA (Hml), mRNA 
0   NM_134810.2  CG15385-RA (CG15385), mRNA 
0   NM_142744.1  CG13410-RA (mRpL35), mRNA 
0   NM_166127.1  CG18255-RF, transcript variant F (Strn-Mlck), mRNA 
0   NM_135201.1  CG9595-RA (osm-6), mRNA 
0   NM_057472.2  CG3299-RA (Vinc), mRNA 
0   NM_078717.2  CG3696-RA, transcript variant A (kis), mRNA 
0   NM_166823.1  CG11059-RB, transcript variant B (cals), mRNA 
0   NM_079893.2  CG11059-RA, transcript variant A (cals), mRNA 
0   NM_164376.1  CG3696-RB, transcript variant B (kis), mRNA 
0   NM_143098.2  CG13664-RA (Cad96Cb), mRNA 
0   NM_137879.2  CG9825-RA (CG9825), mRNA 
0   NM_143788.3  CG13397-RA (CG13397), mRNA 
0   NM_166790.2  CG32019-RA, transcript variant A (bt), mRNA 
0   NM_205877.1  CG32019-RD, transcript variant D (bt), mRNA 
0   NM_206524.1  CG17269-RA (Fancd2), mRNA 
0   NM_205875.1  CG32019-RE, transcript variant E (bt), mRNA 
0   NR_001660.1  CR31808, miscRNA 
0   NM_170484.1  CG1469-RC, transcript variant C (Fer2LCH), mRNA 
0   NM_079981.2  CG1469-RC, transcript variant C (Fer2LCH), mRNA, type II CG8856-RA (Sr-CII), mRNA 
0   NM_058032.3  CG12389-RA (Fpps), mRNA 

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