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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 17943R-3 
 Symbol comm  Full Name commissureless 
 CG No CG17943  Old CG No CG17943 
 Synonyms CG17943, comm, Comm 
 Accession No (Link to NCBI) NM_079371.2 
 Inserted Chr. lll 
 Insertional Mutation  lethal 
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgGCAGCATGAGTCCCGCTGCAA 
 Primer Seq. 3' 
aatctagaggtaccTCTGCTGGCATCGCAACCAAC 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 GCAGCATGAG TCCCGCTGCA ATTGCCGAGC ACCTGCAGCA GAATCAGATC ACCTTCGAGA 
0061 TACCCAGTGC CCACGATCTG CGACACATCG ACGCCCTCAA CTCCTTCANA CGCCCTGCTC 
0121 CAGAGGATTG GCAACGCAGC GGTGTCCTAC GATCCCGCTC CACCCAGCGG CTGGTCTCCG 
0181 GATGGCAGCA TCAGCACCGA GCAGCTCTCC AAGTCGGTGG TTCTGGATCT GGCCGACTTG 
0241 CGGGATAGGT CCGAGGAATC GGGCGAATCC TCGTGGTGGA GCCAGATCTT CGGGGATGCC 
0301 GACATGCATG TGATCATCAA TTATCTGTGG ATCGGAGTGG TCAGCTCGCT GGTCATTCTG 
0361 TCGCTGGTCT TCATCCTCTT CAGCTGCTAC TTCTACAGGA AGTTCCGCAC TTGGAAAAAA 
0421 TGCAATAAGG ACATACGTGC CCAGATNCAT GCGGCCAGTG ACTCGTACTC CTCGCACCTG 
0481 GTTGGTTgNG ATGCCAGCAG A 
 in silico PCR Fragment 
0001 GCAGCATGAG TCCCGCTGCA ATTGCCGAGC ACCTGCAGCA GAATCAGATC ACCTTCGAGA 
0061 TACCCAGTGC CCACGATCTG CGACACATCG ACGCCCTCAA CTCCTTCA-A CGCCCTGCTC 
0121 CAGAGGATTG GCAACGCAGC GGTGTCCTAC GATCCCGCTC CACCCAGCGG CTGGTCTCCG 
0181 GATGGCAGCA TCAGCACCGA GCAGCTCTCC AAGTCGGTGG TTCTGGATCT GGCCGACTTG 
0241 CGGGATAGGT CCGAGGAATC GGGCGAATCC TCGTGGTGGA GCCAGATCTT CGGGGATGCC 
0301 GACATGCATG TGATCATCAA TTATCTGTGG ATCGGAGTGG TCAGCTCGCT GGTCATTCTG 
0361 TCGCTGGTCT TCATCCTCTT CAGCTGCTAC TTCTACAGGA AGTTCCGCAC TTGGAAAAAA 
0421 TGCAATAAGG ACATACGTGC CCAGATCCAT GCGGCCAGTG ACTCGTACTC CTCGCACCTG 
0481 GTTGGTTGCG ATGCCAGCAG A 
 Assemble Data 

17943R-3.IR_full       1   GCAGCATGAGTCCCGCTGCAATTGCCGAGCACCTGCAGCAGAATCAGATCACCTTCGAGA 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17943R-3.in silico     1   GCAGCATGAGTCCCGCTGCAATTGCCGAGCACCTGCAGCAGAATCAGATCACCTTCGAGA 60


17943R-3.IR_full       61  TACCCAGTGCCCACGATCTGCGACACATCGACGCCCTCAACTCCTTCANACGCCCTGCTC 120
                           |||||||||||||||||||||||||||||||||||||||||||||||| |||||||||||
17943R-3.in silico     61  TACCCAGTGCCCACGATCTGCGACACATCGACGCCCTCAACTCCTTCA-ACGCCCTGCTC 120


17943R-3.IR_full       121 CAGAGGATTGGCAACGCAGCGGTGTCCTACGATCCCGCTCCACCCAGCGGCTGGTCTCCG 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17943R-3.in silico     121 CAGAGGATTGGCAACGCAGCGGTGTCCTACGATCCCGCTCCACCCAGCGGCTGGTCTCCG 180


17943R-3.IR_full       181 GATGGCAGCATCAGCACCGAGCAGCTCTCCAAGTCGGTGGTTCTGGATCTGGCCGACTTG 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17943R-3.in silico     181 GATGGCAGCATCAGCACCGAGCAGCTCTCCAAGTCGGTGGTTCTGGATCTGGCCGACTTG 240


17943R-3.IR_full       241 CGGGATAGGTCCGAGGAATCGGGCGAATCCTCGTGGTGGAGCCAGATCTTCGGGGATGCC 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17943R-3.in silico     241 CGGGATAGGTCCGAGGAATCGGGCGAATCCTCGTGGTGGAGCCAGATCTTCGGGGATGCC 300


17943R-3.IR_full       301 GACATGCATGTGATCATCAATTATCTGTGGATCGGAGTGGTCAGCTCGCTGGTCATTCTG 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17943R-3.in silico     301 GACATGCATGTGATCATCAATTATCTGTGGATCGGAGTGGTCAGCTCGCTGGTCATTCTG 360


17943R-3.IR_full       361 TCGCTGGTCTTCATCCTCTTCAGCTGCTACTTCTACAGGAAGTTCCGCACTTGGAAAAAA 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17943R-3.in silico     361 TCGCTGGTCTTCATCCTCTTCAGCTGCTACTTCTACAGGAAGTTCCGCACTTGGAAAAAA 420


17943R-3.IR_full       421 TGCAATAAGGACATACGTGCCCAGATNCATGCGGCCAGTGACTCGTACTCCTCGCACCTG 480
                           |||||||||||||||||||||||||| |||||||||||||||||||||||||||||||||
17943R-3.in silico     421 TGCAATAAGGACATACGTGCCCAGATCCATGCGGCCAGTGACTCGTACTCCTCGCACCTG 480


17943R-3.IR_full       481 GTTGGTTGNGATGCCAGCAGA 501
                           |||||||| ||||||||||||
17943R-3.in silico     481 GTTGGTTGCGATGCCAGCAGA 501



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_079371.2  CG17943-RA (comm), mRNA 
0   0  4  2  4  NM_142685.2  CG7034-RA (sec15), mRNA 
0   0  3  2  2  NM_163888.1  CG32491-RK, transcript variant K (mod(mdg4)), mRNA 
0   0  2  3  5  NM_057611.4  CG3938-RA, transcript variant A (CycE), mRNA 
0   0  2  3  5  NM_057612.4  CG3938-RB, transcript variant B (CycE), mRNA 
0   0  2  3  4  NM_165123.2  CG3938-RC, transcript variant C (CycE), mRNA 
0   0  2  3  4  NM_165125.2  CG3938-RE, transcript variant E (CycE), mRNA 
0   0  2  3  4  NM_165124.2  CG3938-RD, transcript variant D (CycE), mRNA 
0   0  2  3  3  NM_169528.1  CG16901-RB, transcript variant B (sqd), mRNA 
0   0  2  3  3  NM_206481.1  CG16901-RD, transcript variant D (sqd), mRNA 
0   0  2  3  3  NM_143952.1  CG16901-RC, transcript variant C (sqd), mRNA 
0   0  2  2  4  NM_142519.1  CG6011-RA (Prp18), mRNA 
0   0  2  2  2  NM_166076.1  CG12424-RB, transcript variant B (CG12424), mRNA 
0   0  2  2  2  NM_137165.1  CG12424-RA, transcript variant A (CG12424), mRNA 
0   0  2  2  2  NM_166077.1  CG12424-RC, transcript variant C (CG12424), mRNA 
0   0  2  2  2  NM_141281.2  CG2087-RA (PEK), mRNA 
0   0  1  5  6  NM_170178.1  CG31381-RA (CG31381), mRNA 
0   0  1  4  9  NM_141762.2  CG14692-RA (CG14692), mRNA 
0   0  1  4  4  NM_166961.1  CG32498-RI, transcript variant I (dnc), mRNA 
0   0  1  4  4  NM_166965.1  CG32498-RJ, transcript variant J (dnc), mRNA 
0   0  1  4  4  NM_166963.1  CG32498-RK, transcript variant K (dnc), mRNA 
0   0  1  4  4  NM_166964.1  CG32498-RD, transcript variant D (dnc), mRNA 
0   0  1  4  4  NM_166962.1  CG32498-RC, transcript variant C (dnc), mRNA 
0   0  1  4  3  NM_166960.1  CG32498-RB, transcript variant B (dnc), mRNA 
0   0  1  4  3  NM_166966.1  CG32498-RM, transcript variant M (dnc), mRNA 
0   0  1  4  3  NM_166967.1  CG32498-RA, transcript variant A (dnc), mRNA 
0   0  1  4  3  NM_166969.1  CG32498-RE, transcript variant E (dnc), mRNA 
0   0  1  4  3  NM_166968.1  CG32498-RN, transcript variant N (dnc), mRNA 
0   0  1  4  3  NM_166970.1  CG32498-RG, transcript variant G (dnc), mRNA 
0   0  1  4  3  NM_080322.2  CG32498-RF, transcript variant F (dnc), mRNA 

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