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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 17923R-3 
 Symbol JYalpha  Full Name JYalpha 
 CG No CG17923  Old CG No CG17923 
 Synonyms JYalpha, JYAlpha, CG17923 
 Accession No (Link to NCBI) NM_143896.2 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgACGTTTCTAAGCAGGCGGCAG 
 Primer Seq. 3' 
aatctagaggtaccCCATTCTTGTCCGTAGCCATC 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 ACGTTTCTAA GCAGGCGGCA GATATGATTC TATTGGATGA CAACTTTGCA TCAATTGTTG 
0061 TTGGTATTGA AGAGGGGCGG ATTATTTTCG ATAATCTTAA AAAGTCCATC GCATATCACC 
0121 TTGACTTCAA ATCTTCCTGA AATAGTGCCG TTTTTATTTT TTGTGATATT TGATATACCT 
0181 TTAGCTCTGG GCACTATAGC AATTCTATGC ATCGATATCG GCACTGATAT GCTTCCGGCA 
0241 ATATCGCTAG CTTATGAAAA AGCTGAATCT GATATAATGG CGCGTATGCC AAGAGATCCG 
0301 TTTGAAGACC GTTTGGTAAA TAAAAAGTTA ATTCTAATGG CTTATTTGCA AATTGGAGTT 
0361 ATACAAACAG TAGCATGTTT TTTTACTTTT TTTGCTATAA TGGCAGAACA TGGATTCCCC 
0421 CCCTCCAGAC TTAAAGGAAT TCGAGAAGAC TGGGACTCAA AAAAATGTAG AAGACCTTGA 
0481 AGATGGCTAC GGACAAGAAT GG 
 in silico PCR Fragment 
0001 ACGTTTCTAA GCAGGCGGCA GATATGATTC TATTGGATGA CAACTTTGCA TCAATTGTTG 
0061 TTGGTATTGA AGAGGGGCGG ATTATTTTCG ATAATCTTAA AAAGTCCATC GCATAT-ACC 
0121 TTGACTTCAA ATCTTCCTGA AATAGTGCCG TTTTTATTTT TTGTGATATT TGATATACCT 
0181 TTAGCTCTGG GCACTATAGC AATTCTATGC ATCGATATCG GCACTGATAT GCTTCCGGCA 
0241 ATATCGCTAG CTTATGAAAA AGCTGAATCT GATATAATGG CGCGTATGCC AAGAGATCCG 
0301 TTTGAAGACC GTTTGGTAAA TAAAAAGTTA ATTCTAATGG CTTATTTGCA AATTGGAGTT 
0361 ATACAAACAG TAGCATGTTT TTTTACTTTT TTTGCTATAA TGGCAGAACA TGGATTCCCC 
0421 CCCTCCAGAC TTAAAGGAAT TCGAGAAGAC TGGGACTC-A AAAAATGTAG AAGACCTTGA 
0481 AGATGGCTAC GGACAAGAAT GG 
 Assemble Data 

17923R-3.IR_full       1   ACGTTTCTAAGCAGGCGGCAGATATGATTCTATTGGATGACAACTTTGCATCAATTGTTG 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17923R-3.in silico     1   ACGTTTCTAAGCAGGCGGCAGATATGATTCTATTGGATGACAACTTTGCATCAATTGTTG 60


17923R-3.IR_full       61  TTGGTATTGAAGAGGGGCGGATTATTTTCGATAATCTTAAAAAGTCCATCGCATATCACC 120
                           |||||||||||||||||||||||||||||||||||||||||||||||||||||||| |||
17923R-3.in silico     61  TTGGTATTGAAGAGGGGCGGATTATTTTCGATAATCTTAAAAAGTCCATCGCATAT-ACC 120


17923R-3.IR_full       121 TTGACTTCAAATCTTCCTGAAATAGTGCCGTTTTTATTTTTTGTGATATTTGATATACCT 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17923R-3.in silico     121 TTGACTTCAAATCTTCCTGAAATAGTGCCGTTTTTATTTTTTGTGATATTTGATATACCT 180


17923R-3.IR_full       181 TTAGCTCTGGGCACTATAGCAATTCTATGCATCGATATCGGCACTGATATGCTTCCGGCA 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17923R-3.in silico     181 TTAGCTCTGGGCACTATAGCAATTCTATGCATCGATATCGGCACTGATATGCTTCCGGCA 240


17923R-3.IR_full       241 ATATCGCTAGCTTATGAAAAAGCTGAATCTGATATAATGGCGCGTATGCCAAGAGATCCG 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17923R-3.in silico     241 ATATCGCTAGCTTATGAAAAAGCTGAATCTGATATAATGGCGCGTATGCCAAGAGATCCG 300


17923R-3.IR_full       301 TTTGAAGACCGTTTGGTAAATAAAAAGTTAATTCTAATGGCTTATTTGCAAATTGGAGTT 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17923R-3.in silico     301 TTTGAAGACCGTTTGGTAAATAAAAAGTTAATTCTAATGGCTTATTTGCAAATTGGAGTT 360


17923R-3.IR_full       361 ATACAAACAGTAGCATGTTTTTTTACTTTTTTTGCTATAATGGCAGAACATGGATTCCCC 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17923R-3.in silico     361 ATACAAACAGTAGCATGTTTTTTTACTTTTTTTGCTATAATGGCAGAACATGGATTCCCC 420


17923R-3.IR_full       421 CCCTCCAGACTTAAAGGAATTCGAGAAGACTGGGACTCAAAAAAATGTAGAAGACCTTGA 480
                           |||||||||||||||||||||||||||||||||||||| |||||||||||||||||||||
17923R-3.in silico     421 CCCTCCAGACTTAAAGGAATTCGAGAAGACTGGGACTC-AAAAAATGTAGAAGACCTTGA 480


17923R-3.IR_full       481 AGATGGCTACGGACAAGAATGG 502
                           ||||||||||||||||||||||
17923R-3.in silico     481 AGATGGCTACGGACAAGAATGG 502



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  2  NM_143896.2  CG17923-RA (CG17923), mRNA 
0   0  2  2  3  NM_137045.2  CG13335-RB, transcript variant B (CG13335), mRNA 
0   0  1  4  5  NM_168094.1  CG32243-RA (CG32243), mRNA 
0   0  1  4  4  NM_001032080.1  CG33695-RA, transcript variant A (CG33695), mRNA 
0   0  1  4  4  NM_001032077.1  CG33695-RE, transcript variant E (CG33695), mRNA 
0   0  1  4  4  NM_001032079.1  CG33695-RC, transcript variant C (CG33695), mRNA 
0   0  1  4  4  NM_001032081.1  CG33695-RF, transcript variant F (CG33695), mRNA 
0   0  1  4  4  NM_001032078.1  CG33695-RD, transcript variant D (CG33695), mRNA 
0   0  1  4  4  NM_001032076.1  CG33694-RA, transcript variant A (cana), mRNA 
0   0  1  4  2  NM_141274.1  CG2669-RA (CG2669), mRNA 
0   0  1  2  5  NR_001708.1  CR30009, miscRNA 
0   0  1  2  2  NM_167356.1  CG1770-RB, transcript variant B (HDAC4), mRNA 
0   0  1  2  2  NM_167708.1  CG32504-RA (CG32504), mRNA 
0   0  1  2  2  NM_132036.1  CG15767-RA (CG15767), mRNA 
0   0  0  14  44  NM_206526.1  CG5670-RG, transcript variant G (Atpalpha), mRNA 
0   0  0  12  43  NM_206525.1  CG5670-RH, transcript variant H (Atpalpha), mRNA 
0   0  0  12  43  NM_206527.1  CG5670-RF, transcript variant F (Atpalpha), mRNA 
0   0  0  12  39  NM_169939.1  CG5670-RD, transcript variant D (Atpalpha), mRNA 
0   0  0  12  37  NM_169938.1  CG5670-RC, transcript variant C (Atpalpha), mRNA 
0   0  0  12  37  NM_169937.1  CG5670-RB, transcript variant B (Atpalpha), mRNA 
0   0  0  12  37  NM_206528.1  CG5670-RE, transcript variant E (Atpalpha), mRNA 
0   0  0  12  37  NM_169936.1  CG5670-RA, transcript variant A (Atpalpha), mRNA 
0   0  0  5  8  NM_166793.2  CG2999-RC, transcript variant C (unc-13), mRNA 
0   0  0  5  8  NM_143692.2  CG2999-RA, transcript variant A (unc-13), mRNA 
0   0  0  5  5  NR_002068.3  CG2999-RA, transcript variant A (unc-13), mRNA, miscRNA 
0   0  0  5  2  NM_165612.1  CG30353-RA (CG30353), mRNA 
0   0  0  4  7  NM_168961.2  CG32451-RB, transcript variant B (SPoCk), mRNA 
0   0  0  4  7  NM_168963.1  CG32451-RA, transcript variant A (SPoCk), mRNA 
0   0  0  4  7  NM_168962.1  CG32451-RC, transcript variant C (SPoCk), mRNA 
0   0  0  4  7  NM_133108.2  CG32542-RA (CG32542), mRNA 
 Wing Disc
GFP
 Puc
 Caspase
DAPI
 Merge
 Figure
JNK signal
N
 
DB
 
DC
 
DM
 
VM
 
VB
 
Caspase-3 signal
N
++ 
DB
+ 
DC
 
DM
 
VM
 
VB
 
Dcompartment   Penetrance
     

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