|
Notice:
|
|
Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
|
|
| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
|
| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
1771R-1 |
| Symbol |
mew |
Full Name |
multiple edematous wings |
| CG No |
CG1771 |
Old CG No |
CG1771 |
| Synonyms |
alphaPS1, PS1, alpha[[PS1]], CG1771, CT5254, alpha-PS1, PS1alpha, aPS1, l(1)G0429, l(1)G0443, mew, PSalpha1 |
| Accession No (Link to NCBI) |
NM_078590.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Park SH, Lee CW, Lee JH, Park JY, Roshandell M, Brennan CA, Choe KM.
Requirement for and polarized localization of integrin proteins during Drosophila wound closure.
Mol Biol Cell (2018) 29(18) 2137-2147
[
PubMed ID = 29995573
]
[
RRC reference
]
Kim MJ, Choe KM.
Basement membrane and cell integrity of self-tissues in maintaining Drosophila immunological tolerance.
PLoS Genet (2014) 10(10) e1004683
[
PubMed ID = 25329560
]
[
RRC reference
]
Nonaka S, Nagaosa K, Mori T, Shiratsuchi A, Nakanishi Y.
Integrin αPS3/βν-mediated phagocytosis of apoptotic cells and bacteria in Drosophila.
J Biol Chem (2013) 288(15) 10374-80
[
PubMed ID = 23426364
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgCAACTGCCGGCTAAGACAGAA |
| Primer Seq. 3' |
aatctagaggtaccTGGTGTCATCGTCCAGTAGGG |
| Predicted Fragment Size |
623 |
| IR fragment full Seq |
0001 CAACTGCCGG CTAAGACAGA ATCTTGGTAT TCTGATAATC CTGCAATGCG TTCTCACTTG
0061 CTACAATTTC AACCTAGAAC AGCGATTGCC CATTGTGAAG TACGGACATC CGCATTCGCA
0121 TTTCGGCTAC TCGGTGGCCA CCCACACTAT TGGCGAGGCC AATGGGCCCA ACAAGACCAA
0181 CTGTGTTTTA GTGGGAGCTC CATTGGATCA AAATCGACAG CCGAACACCA CACACTCTGG
0241 CGCCCTCTGG CGGTGTCCAA TGACACAGCG TTTCGATGAC TGCGAACAAG TGATCACCGA
0301 TGGCAGGCGA AACTTCGACA GCGAAATCCT TTCACCGCCC GGCAATGACG AAATCAAGGA
0361 GGATCAATGG ATGGGCGTTA CGGTGCGTTC GAATCCCCTA CAGGCCAACG GATCCGGCGG
0421 AAAGGTAATC GTCTGCGCCC ATCGCTATAT GTACATCGTC CGAGAGAATC GTTATGGACA
0481 GGGTCTCTGC TATCTCCTAA CGAACGATCT GCAATTCGAA GAGGTCCACG AGCCCTGCAA
0541 AGGACGACCT GTGCAAAGGC AACACGAAGA TTATGGANCT GTGCCAGGCG GGCACATCGG
0601 CTGCCCTACT GGACGATGAC ACCA |
| in silico PCR Fragment |
0001 CAACTGCCGG CTAAGACAGA ATCTTGGTAT TCTGATAATC CTGCAATGCG TTCTCACTTG
0061 CTACAATTTC AACCTAGAAC AGCGATTGCC CATTGTGAAG TACGGACATC CGCATTCGCA
0121 TTTCGGCTAC TCGGTGGCCA CCCACACTAT TGGCGAGGCC AATGGGCCCA ACAAGACCAA
0181 CTGTGTTTTA GTGGGAGCTC CATTGGATCA AAATCGACAG CCGAACACCA CACACTCTGG
0241 CGCCCTCTGG CGGTGTCCAA TGACACAGCG TTTCGATGAC TGCGAACAAG TGATCACCGA
0301 TGGCAGGCGA AACTTCGACA GCGAAATCCT TTCACCGCCC GGCAATGACG AAATCAAGGA
0361 GGATCAATGG ATGGGCGTTA CGGTGCGTTC GAATCCCCTA CAGGCCAACG GATCCGGCGG
0421 AAAGGTAATC GTCTGCGCCC ATCGCTATAT GTACATCGTC CGAGAGAATC GTTATGGACA
0481 GGGTCTCTGC TATCTCCTAA CGAACGATCT GCAATTCGAA GAGGTCCACG AGCCCTGCAA
0541 AGGACGACCT GTGCAAAGGC AACACGAAGA TTATGGA-CT GTGCCAGGCG GGCACATCGG
0601 CTGCCCTACT GGACGATGAC ACCA |
| Assemble Data |
1771R-1.IR_full 1 CAACTGCCGGCTAAGACAGAATCTTGGTATTCTGATAATCCTGCAATGCGTTCTCACTTG 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1771R-1.in silico 1 CAACTGCCGGCTAAGACAGAATCTTGGTATTCTGATAATCCTGCAATGCGTTCTCACTTG 60
1771R-1.IR_full 61 CTACAATTTCAACCTAGAACAGCGATTGCCCATTGTGAAGTACGGACATCCGCATTCGCA 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1771R-1.in silico 61 CTACAATTTCAACCTAGAACAGCGATTGCCCATTGTGAAGTACGGACATCCGCATTCGCA 120
1771R-1.IR_full 121 TTTCGGCTACTCGGTGGCCACCCACACTATTGGCGAGGCCAATGGGCCCAACAAGACCAA 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1771R-1.in silico 121 TTTCGGCTACTCGGTGGCCACCCACACTATTGGCGAGGCCAATGGGCCCAACAAGACCAA 180
1771R-1.IR_full 181 CTGTGTTTTAGTGGGAGCTCCATTGGATCAAAATCGACAGCCGAACACCACACACTCTGG 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1771R-1.in silico 181 CTGTGTTTTAGTGGGAGCTCCATTGGATCAAAATCGACAGCCGAACACCACACACTCTGG 240
1771R-1.IR_full 241 CGCCCTCTGGCGGTGTCCAATGACACAGCGTTTCGATGACTGCGAACAAGTGATCACCGA 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1771R-1.in silico 241 CGCCCTCTGGCGGTGTCCAATGACACAGCGTTTCGATGACTGCGAACAAGTGATCACCGA 300
1771R-1.IR_full 301 TGGCAGGCGAAACTTCGACAGCGAAATCCTTTCACCGCCCGGCAATGACGAAATCAAGGA 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1771R-1.in silico 301 TGGCAGGCGAAACTTCGACAGCGAAATCCTTTCACCGCCCGGCAATGACGAAATCAAGGA 360
1771R-1.IR_full 361 GGATCAATGGATGGGCGTTACGGTGCGTTCGAATCCCCTACAGGCCAACGGATCCGGCGG 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1771R-1.in silico 361 GGATCAATGGATGGGCGTTACGGTGCGTTCGAATCCCCTACAGGCCAACGGATCCGGCGG 420
1771R-1.IR_full 421 AAAGGTAATCGTCTGCGCCCATCGCTATATGTACATCGTCCGAGAGAATCGTTATGGACA 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1771R-1.in silico 421 AAAGGTAATCGTCTGCGCCCATCGCTATATGTACATCGTCCGAGAGAATCGTTATGGACA 480
1771R-1.IR_full 481 GGGTCTCTGCTATCTCCTAACGAACGATCTGCAATTCGAAGAGGTCCACGAGCCCTGCAA 540
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1771R-1.in silico 481 GGGTCTCTGCTATCTCCTAACGAACGATCTGCAATTCGAAGAGGTCCACGAGCCCTGCAA 540
1771R-1.IR_full 541 AGGACGACCTGTGCAAAGGCAACACGAAGATTATGGANCTGTGCCAGGCGGGCACATCGG 600
||||||||||||||||||||||||||||||||||||| ||||||||||||||||||||||
1771R-1.in silico 541 AGGACGACCTGTGCAAAGGCAACACGAAGATTATGGA-CTGTGCCAGGCGGGCACATCGG 600
1771R-1.IR_full 601 CTGCCCTACTGGACGATGACACCA 624
||||||||||||||||||||||||
1771R-1.in silico 601 CTGCCCTACTGGACGATGACACCA 624
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
605 |
0 |
0 |
2 |
NM_078590.2 |
CG1771-RB, transcript variant B (mew), mRNA |
| 69.75 |
422 |
2 |
1 |
5 |
NM_167354.1 |
CG1771-RA, transcript variant A (mew), mRNA |
| 0 |
0 |
4 |
4 |
2 |
NM_166207.1 |
CG6829-RB, transcript variant B (Ark), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_175962.1 |
CG3294-RB, transcript variant B (CG3294), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_135013.2 |
CG3294-RA, transcript variant A (CG3294), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_135211.2 |
CG31634-RA (Oatp26F), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_132539.2 |
CG1559-RA (Upf1), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_164913.1 |
CG13138-RA, transcript variant A (CG13138), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_135528.1 |
CG13138-RB, transcript variant B (CG13138), mRNA |
| 0 |
0 |
1 |
4 |
5 |
NM_134591.2 |
CG1501-RA (unc), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_136615.2 |
CG8058-RA (CG8058), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_079768.2 |
CG6238-RA, transcript variant A (ssh), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_140670.1 |
CG13030-RA (CG13030), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_206200.1 |
CG11206-RB, transcript variant B (CG11206), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_137819.2 |
CG11206-RA, transcript variant A (CG11206), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_057309.2 |
CG6534-RA (slou), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_001015350.1 |
CG40085-PA.3 (CG40085), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_139498.1 |
CG9965-RA (CG9965), mRNA |
| 0 |
0 |
0 |
5 |
5 |
NM_175999.2 |
CG32985-RA (CG32985), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_137801.2 |
CG6203-RA, transcript variant A (Fmr1), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_169324.1 |
CG6203-RC, transcript variant C (Fmr1), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_132622.1 |
CG18646-RA (CG18646), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_168254.1 |
CG7915-RB, transcript variant B (Ect4), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_135360.3 |
CG7830-RA (CG7830), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_144353.2 |
CG7626-RA, transcript variant A (Spt5), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_206167.1 |
CG7626-RB, transcript variant B (Spt5), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_139523.1 |
CG12734-RA, transcript variant A (CG12734), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_206262.1 |
CG12734-RB, transcript variant B (CG12734), mRNA |
| 0 |
0 |
0 |
3 |
15 |
NM_078654.2 |
CG9623-RB, transcript variant B (if), mRNA |
| 0 |
0 |
0 |
3 |
14 |
NM_167544.1 |
CG9623-RA, transcript variant A (if), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
|
JNK signal
|
Caspase-3 signal
| N |
 |
| ++ |
|
| DB |
 |
| + |
|
| DC |
 |
| ++ |
|
| DM |
 |
| + |
|
| VM |
 |
| |
|
| VB |
 |
| |
|
|
|
|
|
|
