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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
1762R-1 |
| Symbol |
betaInt-nu |
Full Name |
beta[nu] integrin |
| CG No |
CG1762 |
Old CG No |
CG1762 |
| Synonyms |
betanu, betav, CG1762, CT5192, beta[[v]], Beta[[nu]], betaInt[nu], betaInt-nu, beta[nu]-integrin |
| Accession No (Link to NCBI) |
NM_078884.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
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Reference
[Please submit your publication]
|
Zhang M, Nagaosa K, Nakai Y, Yasugi T, Kushihiki M, Rahmatika D, Sato M, Shiratsuchi A, Nakanishi Y.
Role for phagocytosis in the prevention of neoplastic transformation in Drosophila.
Genes Cells (2020) 25(10) 675-684
[
PubMed ID = 32865275
]
[
RRC reference
]
Nonaka S, Hori A, Nakanishi Y, Kuraishi T.
Phagocytosis Assay for Apoptotic Cells in Drosophila Embryos.
J Vis Exp (2017) (126)
[
PubMed ID = 28809832
]
[
RRC reference
]
Nonaka S, Ando Y, Kanetani T, Hoshi C, Nakai Y, Nainu F, Nagaosa K, Shiratsuchi A, Nakanishi Y.
Signaling pathway for phagocyte priming upon encounter with apoptotic cells.
J Biol Chem (2017) 292(19) 8059-8072
[
PubMed ID = 28325838
]
[
RRC reference
]
Kim MJ, Choe KM.
Basement membrane and cell integrity of self-tissues in maintaining Drosophila immunological tolerance.
PLoS Genet (2014) 10(10) e1004683
[
PubMed ID = 25329560
]
[
RRC reference
]
Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340
[
PubMed ID = 25473846
]
[
RRC reference
]
Nonaka S, Nagaosa K, Mori T, Shiratsuchi A, Nakanishi Y.
Integrin αPS3/βν-mediated phagocytosis of apoptotic cells and bacteria in Drosophila.
J Biol Chem (2013) 288(15) 10374-80
[
PubMed ID = 23426364
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
Saj A, Arziman Z, Stempfle D, van Belle W, Sauder U, Horn T, Dürrenberger M, Paro R, Boutros M, Merdes G.
A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network.
Dev Cell (2010) 18(5) 862-76
[
PubMed ID = 20493818
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGGAATACCAGGTGGGATATCG |
| Primer Seq. 3' |
aatctagaggtaccCTGGTATATCATCGGTTAGCG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 GGAATACCAG GTGGGATATC GTTGTCTCTC TCGCCGGCAA CTACTTAACT ACAACTGCAG
0061 TGAAACGTGA TATATACGAG AACCAGCCGG TCCTAGATGT CCTTCAGGAT AAGCCCCTAA
0121 AGGACTACGA AACCAGTGAT CAGGCTGTCC AAGTCACACC CCAGCGGGCA TACCTTAAGT
0181 TGGTCAAAGG AAACAAGCAA AGAATGAAGC TGAGCTATAG AACTGCACGC AACAATCCAC
0241 TCGATTTGTA CGTATTGATG GATCTAACCT GGACAATGAG GGATGACAAG AAAACCCTGG
0301 AGGAGTTGGG AGCCCAACTC AGTCAGACTC TTAAAAATCT AACAGGAAAC TACAGATTGG
0361 GATTTGGTTC TTTCGCGGAC AAGCCAACTC TACCCATGAT TCTGCCACAG CATAGGGAAA
0421 ACCCCTGTGC TGCGGAGCGG GCCACGTGTG AACCAACCTA TGGATATAGA CATCAACTTT
0481 CGCTAACCGA TGATATACCA G |
| in silico PCR Fragment |
0001 GGAATACCAG GTGGGATATC GTTGTCTCTC TCGCCGGCAA CTACTTAACT ACAACTGCAG
0061 TGAAACG-GA TATATACGAG AACCAGCCGG TCCTAGATGT CCTTCAGGAT AAGCCCCTAA
0121 AGGACTACGA AACCAGTGAT CAGGCTGTCC AAGTCACACC CCAGCGGGCA TACCTTAAGT
0181 TGGTCAAAGG AAACACGCAA AGAATGAAGC TGAGCTATAG AACTGCACGC AACAATCCAC
0241 TCGATTTGTA CGTATTGATG GATCTAACCT GGACAATGAG GGATGACAAG AAAACCCTGG
0301 AGGAGTTGGG AGCCCAACTC AGTCAGACTC TTAAAAATCT AACAGGAAAC TACAGATTGG
0361 GATTTGGTTC TTTCGCGGAC AAGCCAACTC TACCCATGAT TCTGCCACAG CATAGGGAAA
0421 ACCCCTGTGC TGCGGAGCGG GCCACGTGTG AACCAACCTA TGGATATAGA CATCAACTTT
0481 CGCTAACCGA TGATATACCA G |
| Assemble Data |
1762R-1.IR_full 1 GGAATACCAGGTGGGATATCGTTGTCTCTCTCGCCGGCAACTACTTAACTACAACTGCAG 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1762R-1.in silico 1 GGAATACCAGGTGGGATATCGTTGTCTCTCTCGCCGGCAACTACTTAACTACAACTGCAG 60
1762R-1.IR_full 61 TGAAACGTGATATATACGAGAACCAGCCGGTCCTAGATGTCCTTCAGGATAAGCCCCTAA 120
||||||| ||||||||||||||||||||||||||||||||||||||||||||||||||||
1762R-1.in silico 61 TGAAACG-GATATATACGAGAACCAGCCGGTCCTAGATGTCCTTCAGGATAAGCCCCTAA 120
1762R-1.IR_full 121 AGGACTACGAAACCAGTGATCAGGCTGTCCAAGTCACACCCCAGCGGGCATACCTTAAGT 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1762R-1.in silico 121 AGGACTACGAAACCAGTGATCAGGCTGTCCAAGTCACACCCCAGCGGGCATACCTTAAGT 180
1762R-1.IR_full 181 TGGTCAAAGGAAACAAGCAAAGAATGAAGCTGAGCTATAGAACTGCACGCAACAATCCAC 240
||||||||||||||| ||||||||||||||||||||||||||||||||||||||||||||
1762R-1.in silico 181 TGGTCAAAGGAAACACGCAAAGAATGAAGCTGAGCTATAGAACTGCACGCAACAATCCAC 240
1762R-1.IR_full 241 TCGATTTGTACGTATTGATGGATCTAACCTGGACAATGAGGGATGACAAGAAAACCCTGG 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1762R-1.in silico 241 TCGATTTGTACGTATTGATGGATCTAACCTGGACAATGAGGGATGACAAGAAAACCCTGG 300
1762R-1.IR_full 301 AGGAGTTGGGAGCCCAACTCAGTCAGACTCTTAAAAATCTAACAGGAAACTACAGATTGG 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1762R-1.in silico 301 AGGAGTTGGGAGCCCAACTCAGTCAGACTCTTAAAAATCTAACAGGAAACTACAGATTGG 360
1762R-1.IR_full 361 GATTTGGTTCTTTCGCGGACAAGCCAACTCTACCCATGATTCTGCCACAGCATAGGGAAA 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1762R-1.in silico 361 GATTTGGTTCTTTCGCGGACAAGCCAACTCTACCCATGATTCTGCCACAGCATAGGGAAA 420
1762R-1.IR_full 421 ACCCCTGTGCTGCGGAGCGGGCCACGTGTGAACCAACCTATGGATATAGACATCAACTTT 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1762R-1.in silico 421 ACCCCTGTGCTGCGGAGCGGGCCACGTGTGAACCAACCTATGGATATAGACATCAACTTT 480
1762R-1.IR_full 481 CGCTAACCGATGATATACCAG 501
|||||||||||||||||||||
1762R-1.in silico 481 CGCTAACCGATGATATACCAG 501
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
2 |
NM_078884.2 |
CG1762-RA (betaInt-nu), mRNA |
| 0 |
0 |
2 |
5 |
2 |
NM_143112.1 |
CG11878-RA (CG11878), mRNA |
| 0 |
0 |
2 |
2 |
7 |
NM_165198.1 |
CG31810-RA (CG31810), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_135472.4 |
CG4128-RC, transcript variant C (nAcRalpha-30D), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_205951.1 |
CG4128-RB, transcript variant B (nAcRalpha-30D), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_164874.2 |
CG4128-RA, transcript variant A (nAcRalpha-30D), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_205953.1 |
CG4128-RE, transcript variant E (nAcRalpha-30D), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_205952.1 |
CG4128-RD, transcript variant D (nAcRalpha-30D), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_001038799.1 |
CG4128-RF, transcript variant F (nAcRalpha-30D), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_142183.1 |
CG4334-RA (CG4334), mRNA |
| 0 |
0 |
0 |
7 |
3 |
NM_144367.1 |
CG6633-RA (Ugt86Dd), mRNA |
| 0 |
0 |
0 |
6 |
5 |
NM_137081.2 |
CG30483-RA (Prosap), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_137140.2 |
CG10151-RC, transcript variant C (CG10151), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_166068.1 |
CG10151-RB, transcript variant B (CG10151), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_166067.1 |
CG10151-RA, transcript variant A (CG10151), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_176542.1 |
CG7029-RA (CG7029), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_134751.1 |
CG5556-RA (CG5556), mRNA |
| 0 |
0 |
0 |
3 |
11 |
NM_001031986.1 |
CG33719-RB, transcript variant B (Pif1A), mRNA |
| 0 |
0 |
0 |
3 |
5 |
NM_166125.2 |
CG18255-RA, transcript variant A (Strn-Mlck), mRNA |
| 0 |
0 |
0 |
3 |
5 |
NM_166129.2 |
CG18255-RD, transcript variant D (Strn-Mlck), mRNA |
| 0 |
0 |
0 |
3 |
5 |
NM_132990.2 |
CG8557-RA, transcript variant A (CG8557), mRNA |
| 0 |
0 |
0 |
3 |
5 |
NM_176751.1 |
CG8557-RB, transcript variant B (CG8557), mRNA |
| 0 |
0 |
0 |
3 |
4 |
NM_134731.2 |
CG4764-RA (CG4764), mRNA |
| 0 |
0 |
0 |
3 |
3 |
NM_139513.2 |
CG7740-RC, transcript variant C (prominin-like), mRNA |
| 0 |
0 |
0 |
3 |
3 |
NM_132395.1 |
CG2898-RA (CG2898), mRNA |
| 0 |
0 |
0 |
3 |
3 |
NM_167986.1 |
CG7740-RA, transcript variant A (prominin-like), mRNA |
| 0 |
0 |
0 |
3 |
3 |
NM_141170.2 |
CG11115-RA (Ssl1), mRNA |
| 0 |
0 |
0 |
3 |
3 |
NM_206021.1 |
CG1374-RB, transcript variant B (tsh), mRNA |
| 0 |
0 |
0 |
3 |
3 |
NM_167987.1 |
CG7740-RB, transcript variant B (prominin-like), mRNA |
| 0 |
0 |
0 |
3 |
3 |
NM_176504.1 |
CG33207-RB, transcript variant B (pxb), mRNA |
|
| Wing Disc |
GFP
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Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
JNK signal
| N |
 |
| + |
|
| DB |
 |
| +- |
|
| DC |
 |
| + |
|
| DM |
 |
| +- |
|
| VM |
 |
| |
|
| VB |
 |
| |
|
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Caspase-3 signal
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