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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 17593R-1 
 Symbol CG17593  Full Name CG17593 
 CG No CG17593  Old CG No CG17593 
 Synonyms CG17593 
 Accession No (Link to NCBI) NM_134942.2 
 Inserted Chr. lll 
 Insertional Mutation  lethal 
 Phenotype induced by Act5C-GAL4 at 28 degrees semi-lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgCTCATCTGCTTCGCCAGCACT 
 Primer Seq. 3' 
aatctagaggtaccAGGGCTCTGTGGGTACTGAAC 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 CTCATCTGCT TCgCCAGCAC TAAGTCGGAG AGCGACTTCG AGGAAGACGA CAGTGATGAT 
0061 TTTGCCGAAT TCGACGACGG CAATTTTTAC GCTGAGCCAG CGGCCAAGAA TGCAGATTCG 
0121 TTCTCCGGAG GAGCAGNGAT CCAGCCACGG CGAGTCCCGC GACGATCCGC CTGCCAAGGA 
0181 GCCGCGGAAG CAAGCACAAA ACCAGGAGAG CGACGAGGAG GACGGCCTTG TCGAGGATGA 
0241 CGATGAGTTT GAGCACTTCC AGGATGAGGA AGAGTTCGAG GGCTACGATG GCGGAGACTC 
0301 CAAGGATCCG CCCATCGACC AAAAGGTTGA GCCAAAACTG AACATACCAA ACATCCCTCT 
0361 GCACTTCCGC ACCCACTGGG ACTCCTACTG GATGGAGATG CTCATGGTGG CAGGGTTGTT 
0421 GGCCTACTTC GCCAACTTCT TTGCGGGAAA GGCCAAAAAC GCGCGTTTgG NGCAGCTTTG 
0481 GTTCAGTACC CACAGAGCCC T 
 in silico PCR Fragment 
0001 CTCATCTGCT TCGCCAGCAC TAAGTCGGAG AGCGACTTCG AGGAAGACGA CAGTGATGAT 
0061 TTTGCCGAAT TCGACGACGG CAATTTTTAC GCTGAGCCAG CGGCCAAGAA TGCAGATTCG 
0121 TTCTCCGGAG GAGCAG-GAT CCAGCCACGG CGAGTCCCGC GACGATCCGC CTGCCAAGGA 
0181 GCCGCGGAAG CAAGCACAAA ACCAGGAGAG CGACGAGGAG GACGGCCTTG TCGAGGATGA 
0241 CGATGAGTTT GAGCACTTCC AGGATGAGGA AGAGTTCGAG GGCTACGATG GCGGAGACTC 
0301 CAAGGATCCG CCCATCGACC AAAAGGTTGA GCCAAAACTG AACATACCAA ACATCCCTCT 
0361 GCACTTCCGC ACCCACTGGG ACTCCTACTG GATGGAGATG CTCATGGTGG CAGGGTTGTT 
0421 GGCCTACTTC GCCAACTTCT TTGCGGGAAA GGCCAAAAAC GCGCGTTTGG CGCAGCTTTG 
0481 GTTCAGTACC CACAGAGCCC T 
 Assemble Data 

3213R-3.IR_full       1   CGGCCATGTACATGGAGTACCTGGCGCTGATCAAGGAGATCGATTGCCAGAACAAGATCA 60
                          ||  ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3213R-3.in silico     1   CG--CATGTACATGGAGTACCTGGCGCTGATCAAGGAGATCGATTGCCAGAACAAGATCA 60


3213R-3.IR_full       61  TCCAGGACATCAAGAGCAACATCATGGACATGTGCGCCAAGCCCTGCAAGACCCGCAACG 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3213R-3.in silico     61  TCCAGGACATCAAGAGCAACATCATGGACATGTGCGCCAAGCCCTGCAAGACCCGCAACG 120


3213R-3.IR_full       121 AACTGCGCGAGATCAAGCGGTTGAGGATTTGCATGGAGCAGGAGAACATTAAGCTGCACA 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3213R-3.in silico     121 AACTGCGCGAGATCAAGCGGTTGAGGATTTGCATGGAGCAGGAGAACATTAAGCTGCACA 180


3213R-3.IR_full       181 CCATGATGAACAGAGCGGTGCAGCTGCAAAACTTCGGCTCCCATCGGCACTACAAGGAGC 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3213R-3.in silico     181 CCATGATGAACAGAGCGGTGCAGCTGCAAAACTTCGGCTCCCATCGGCACTACAAGGAGC 240


3213R-3.IR_full       241 TGGCCATGACCACCACCGTCGACGAGGACAACCTGTCACCGTATATGTGTGGCATCGGTC 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3213R-3.in silico     241 TGGCCATGACCACCACCGTCGACGAGGACAACCTGTCACCGTATATGTGTGGCATCGGTC 300


3213R-3.IR_full       301 CTTGTGCTCCAGCTGGCAGCGCCACGGATCCCTGTAGCGAAGAGCCTAGCTCGTGTGCCA 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3213R-3.in silico     301 CTTGTGCTCCAGCTGGCAGCGCCACGGATCCCTGTAGCGAAGAGCCTAGCTCGTGTGCCA 360


3213R-3.IR_full       361 GTGTTTGTGGTGGCGGCGGAGGCGGTGGAGGTCAACGGGACATGGCTGGTCAGGACGACA 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3213R-3.in silico     361 GTGTTTGTGGTGGCGGCGGAGGCGGTGGAGGTCAACGGGACATGGCTGGTCAGGACGACA 420


3213R-3.IR_full       421 AGTTGATCTGCGCTCTGCAAAAGGCCATGCAGAAGATGAAGGGCGCTTGTCCTGAGGACA 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3213R-3.in silico     421 AGTTGATCTGCGCTCTGCAAAAGGCCATGCAGAAGATGAAGGGCGCTTGTCCTGAGGACA 480


3213R-3.IR_full       481 AGAAGATGATCGAAGAGATCGC 502
                          ||||||||||||||||||||||
3213R-3.in silico     481 AGAAGATGATCGAAGAGATCGC 502



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch 		 

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