NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 17562R-1

Symbol CG17562 Full Name CG17562

CG No CG17562 Old CG No CG17562

Synonyms CG17562

Accession No (Link to NCBI) NM_142309.1

Inserted Chr. lll

Insertional Mutation lethal

Phenotype induced by Act5C-GAL4 at 28 degrees viable

Map Viewer

Reference
[Please submit your publication] Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgAAGATGCTTCCGGAGCAAAAC

Primer Seq. 3'

aatctagaggtaccTGAGATGCTCGGGGTAGAAAT

Predicted Fragment Size

IR fragment full Seq

0001 AAGATGCTTC CGGAGCAAAA CGGTGTTCCT AACTGGAGCC ACCGGCTTTT TGGGTAAAGT 
0061 CGTTATTGAG AAACTTTTGC GGACCACAGA GGTGAAACGC ATCTATGTGC TAATTAGACC 
0121 AAAGCGCGGA GTTGAGATTA AGGATCGGAT TATCACCTGG TCAAAAGATG CGGTATTTGA 
0181 ACTGCTGCTG AAGTCCAAGC CGGAGGCACT TCAAAGAGTA TGTCCCATCG CTGGGGATTG 
0241 CCTCGACTTT GATTTGGGAA TCAGCGAGAA TGATCGAAGA CTTCTGGCCT CCGAGGTGCA 
0301 GATTGTCATC CATGGAGCTG CCACCGTGCG ATTCAATGAG CCGCTTGCAC GTgGCCCTGG 
0361 CCATCAACAC AAGGGCCACC AAGCTGATGA TTCAACTGGC CAAGGAAATG AGGCAGCTGG 
0421 AGGCCTTCGT TCATGTATCG ACCGCCTTCT CCAACTGCGT TATCTACAAC ATCAAGGAAA 
0481 ATTTCTACCC CGAGCATCTC A

in silico PCR Fragment

0001 AAGATGCTTC CGGAGCAAAA CGGTGTTCCT AACTGGAGCC ACCGGCTTTT TGGGTAAAGT 
0061 CGTTATTGAG AAACTTTTGC GGACCACAGA GGTGAAACGC ATCTATGTGC TAATTAGACC 
0121 AAAGCGCGGA GTTGAGATTA AGGATCGGAT TATCACCTGG TCAAAAGATG CGGTATTTGA 
0181 ACTGCTGCTG AAGTCCAAGC CGGAGGCACT TCAAAGAGTA TGTCCCATCG CTGGGGATTG 
0241 CCTCGACTTT GATTTGGGAA TCAGCGAGAA TGATCGAAGA CTTCTGGCCT CCGAGGTGCA 
0301 GATTGTCATC CATGGAGCTG CCACCGTGCG ATTCAATGAG CCGCTT-CAC GTGGCCCTGG 
0361 CCATCAACAC AAGGGCCACC AAGCTGATGA TTCAACTGGC CAAGGAAATG AGGCAGCTGG 
0421 AGGCCTTCGT TCATGTATCG ACCGCCTTCT CCAACTGCGT TATCTACAAC ATCAAGGAAA 
0481 ATTTCTACCC CGAGCATCTC A

Assemble Data


17562R-1.IR_full       1   AAGATGCTTCCGGAGCAAAACGGTGTTCCTAACTGGAGCCACCGGCTTTTTGGGTAAAGT 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17562R-1.in silico     1   AAGATGCTTCCGGAGCAAAACGGTGTTCCTAACTGGAGCCACCGGCTTTTTGGGTAAAGT 60


17562R-1.IR_full       61  CGTTATTGAGAAACTTTTGCGGACCACAGAGGTGAAACGCATCTATGTGCTAATTAGACC 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17562R-1.in silico     61  CGTTATTGAGAAACTTTTGCGGACCACAGAGGTGAAACGCATCTATGTGCTAATTAGACC 120


17562R-1.IR_full       121 AAAGCGCGGAGTTGAGATTAAGGATCGGATTATCACCTGGTCAAAAGATGCGGTATTTGA 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17562R-1.in silico     121 AAAGCGCGGAGTTGAGATTAAGGATCGGATTATCACCTGGTCAAAAGATGCGGTATTTGA 180


17562R-1.IR_full       181 ACTGCTGCTGAAGTCCAAGCCGGAGGCACTTCAAAGAGTATGTCCCATCGCTGGGGATTG 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17562R-1.in silico     181 ACTGCTGCTGAAGTCCAAGCCGGAGGCACTTCAAAGAGTATGTCCCATCGCTGGGGATTG 240


17562R-1.IR_full       241 CCTCGACTTTGATTTGGGAATCAGCGAGAATGATCGAAGACTTCTGGCCTCCGAGGTGCA 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17562R-1.in silico     241 CCTCGACTTTGATTTGGGAATCAGCGAGAATGATCGAAGACTTCTGGCCTCCGAGGTGCA 300


17562R-1.IR_full       301 GATTGTCATCCATGGAGCTGCCACCGTGCGATTCAATGAGCCGCTTGCACGTGGCCCTGG 360
                           |||||||||||||||||||||||||||||||||||||||||||||| |||||||||||||
17562R-1.in silico     301 GATTGTCATCCATGGAGCTGCCACCGTGCGATTCAATGAGCCGCTT-CACGTGGCCCTGG 360


17562R-1.IR_full       361 CCATCAACACAAGGGCCACCAAGCTGATGATTCAACTGGCCAAGGAAATGAGGCAGCTGG 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17562R-1.in silico     361 CCATCAACACAAGGGCCACCAAGCTGATGATTCAACTGGCCAAGGAAATGAGGCAGCTGG 420


17562R-1.IR_full       421 AGGCCTTCGTTCATGTATCGACCGCCTTCTCCAACTGCGTTATCTACAACATCAAGGAAA 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17562R-1.in silico     421 AGGCCTTCGTTCATGTATCGACCGCCTTCTCCAACTGCGTTATCTACAACATCAAGGAAA 480


17562R-1.IR_full       481 ATTTCTACCCCGAGCATCTCA 501
                           |||||||||||||||||||||
17562R-1.in silico     481 ATTTCTACCCCGAGCATCTCA 501

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_142309.1	CG17562-RA (CG17562), mRNA
0.41	2	14	15	45	NM_141929.2	CG10096-RA, transcript variant A (CG10096), mRNA
0.41	2	14	15	45	NM_169464.2	CG10097-RA, transcript variant A (CG10097), mRNA
0.41	2	14	11	20	NM_001032012.1	CG10096-RB, transcript variant B (CG10096), mRNA
0	0	26	28	59	NM_142310.1	CG17560-RA (CG17560), mRNA
0	0	16	49	35	NM_142311.1	CG14893-RA (CG14893), mRNA
0	0	14	6	4	NM_166697.1	CG30427-RC, transcript variant C (CG30427), mRNA
0	0	14	6	4	NM_138136.2	CG30427-RB, transcript variant B (CG30427), mRNA
0	0	14	6	4	NM_166699.1	CG30427-RD, transcript variant D (CG30427), mRNA
0	0	14	6	4	NM_166698.1	CG30427-RA, transcript variant A (CG30427), mRNA
0	0	5	10	5	NM_135397.2	CG13091-RA (CG13091), mRNA
0	0	4	10	9	NM_143764.1	CG18031-RA (CG18031), mRNA
0	0	2	2	2	NM_140564.1	CG17029-RA (CG17029), mRNA
0	0	1	3	4	NM_135960.2	CG17331-RA (CG17331), mRNA
0	0	1	3	3	NM_168382.1	CG6721-RB, transcript variant B (Gap1), mRNA
0	0	1	3	3	NM_079290.2	CG6721-RA, transcript variant A (Gap1), mRNA
0	0	1	3	2	NM_079845.2	CG7951-RA (sima), mRNA
0	0	1	3	2	NM_141848.1	CG6908-RA (CG6908), mRNA
0	0	1	3	2	NM_170360.1	CG4976-RA (Mes-4), mRNA
0	0	1	2	3	NM_142335.2	CG5208-RA (CG5208), mRNA
0	0	0	8	4	NM_169679.1	CG9734-RC, transcript variant C (glob1), mRNA
0	0	0	8	4	NM_169680.1	CG9734-RD, transcript variant D (glob1), mRNA
0	0	0	8	4	NM_079645.3	CG9734-RB, transcript variant B (glob1), mRNA
0	0	0	8	4	NM_169678.1	CG9734-RA, transcript variant A (glob1), mRNA
0	0	0	8	2	NM_057714.3	CG12296-RA (klu), mRNA
0	0	0	4	25	NM_001032011.1	CG10097-RB, transcript variant B (CG10097), mRNA
0	0	0	4	8	NM_136422.2	CG1845-RA (CG1845), mRNA
0	0	0	4	5	NM_001038782.1	CG34049-RA (CG34049), mRNA
0	0	0	4	3	NM_140293.2	CG5645-RA (CG5645), mRNA
0	0	0	4	3	NM_165535.1	CG2146-RC, transcript variant C (didum), mRNA

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