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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 17514R-3 
 Symbol CG17514  Full Name CG17514 
 CG No CG17514  Old CG No CG17514 
 Synonyms CG17514 
 Accession No (Link to NCBI) NM_001015316.1 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624 [ PubMed ID = 21666802 ] [ RRC reference ]

Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgTTTGACTCCGGATGGAGAGTT 
 Primer Seq. 3' 
aatctagaggtaccAGGACGGGCTTTGAAGTTTGA 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 TTTGACTCCG GATGGaNAGT TATTTGATAA AAGTGTAATA CCTCATATAG ACTCGCAATA 
0061 TGAAACAGTT CGACTTAAAA GAGAAAATAA AGTTTACAGC TACAAGGAAC AATTAGAAGA 
0121 GATTCAACTT CGGCGTGAAA TTGACGATAA AAGGGAAAAA GAAGGCAAAT TAAAAACTAT 
0181 AAGGTATACT CAGAAGCAAG AAGAACAAAT TAAAAATCAA ATGGAAAAAG AACTACATAT 
0241 AAAATTAAGA ATTACACTGC TTTATGAAAA ACTTATTTCG AAAATAAGCT TACTAAAAGC 
0301 ATCATGTTCT GGGAATGGAG AACAAATGGC CCAACATTTT TATTCTTTAT TAGACGGCAT 
0361 ATTGAATGCT TCTAAAAGTC CCCTATGTGC CGAAGTTCTT ACTGACCTGT ACATATTTTT 
0421 GCATAATCTG TGCTTTACTT TCCAGCCAAA ACTTGGACGT GCCATCGCAT TAGCGACAAT 
0481 CAAACTTCAA AGCCCGTCCT  
 in silico PCR Fragment 
0001 TTTGACTCCG GATGGAGAGT TATTTGATAA AAGTGTAATA CCTCATATAG ACTCGCAATA 
0061 TGAAACAGTT CGACTTAAAA GAGAAAATAA AGTTTACAGC TACAAGGAAC AATTAGAAGA 
0121 GATTCAACTT CGGCGTGAAA TTGACGATAA AAGGGAAAAA GAAGGCAAAT TAAAAACTAT 
0181 AAGGTATACT CAGAAGCAAG AAGAACAAAT TAAAAATCAA ATGGAAAAAG AACTACATAT 
0241 AAAATTAAGA ATTACACTGC TTTATGAAAA ACTTATTTCG AAAATAAGCT TACTAAAAGC 
0301 ATCATGTTCT GGGAATGGAG AACAAATGGC CCAACATTTT TATTCTTTAT TAGACGGCAT 
0361 ATTGAATGCT TCTAAAAGTC CCCTATGTGC CGAAGTTCTT ACTGACCTGT ACATATTTTT 
0421 GCATAATCTG TGCTTTACTT TCCAGCCAAA ACTTGGACGT GCCATCGCAT TAGCGACAAT 
0481 CAAACTTCAA AGCCCGTCCT  
 Assemble Data 

17514R-3.IR_full       1   TTTGACTCCGGATGGANAGTTATTTGATAAAAGTGTAATACCTCATATAGACTCGCAATA 60
                           |||||||||||||||| |||||||||||||||||||||||||||||||||||||||||||
17514R-3.in silico     1   TTTGACTCCGGATGGAGAGTTATTTGATAAAAGTGTAATACCTCATATAGACTCGCAATA 60


17514R-3.IR_full       61  TGAAACAGTTCGACTTAAAAGAGAAAATAAAGTTTACAGCTACAAGGAACAATTAGAAGA 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17514R-3.in silico     61  TGAAACAGTTCGACTTAAAAGAGAAAATAAAGTTTACAGCTACAAGGAACAATTAGAAGA 120


17514R-3.IR_full       121 GATTCAACTTCGGCGTGAAATTGACGATAAAAGGGAAAAAGAAGGCAAATTAAAAACTAT 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17514R-3.in silico     121 GATTCAACTTCGGCGTGAAATTGACGATAAAAGGGAAAAAGAAGGCAAATTAAAAACTAT 180


17514R-3.IR_full       181 AAGGTATACTCAGAAGCAAGAAGAACAAATTAAAAATCAAATGGAAAAAGAACTACATAT 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17514R-3.in silico     181 AAGGTATACTCAGAAGCAAGAAGAACAAATTAAAAATCAAATGGAAAAAGAACTACATAT 240


17514R-3.IR_full       241 AAAATTAAGAATTACACTGCTTTATGAAAAACTTATTTCGAAAATAAGCTTACTAAAAGC 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17514R-3.in silico     241 AAAATTAAGAATTACACTGCTTTATGAAAAACTTATTTCGAAAATAAGCTTACTAAAAGC 300


17514R-3.IR_full       301 ATCATGTTCTGGGAATGGAGAACAAATGGCCCAACATTTTTATTCTTTATTAGACGGCAT 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17514R-3.in silico     301 ATCATGTTCTGGGAATGGAGAACAAATGGCCCAACATTTTTATTCTTTATTAGACGGCAT 360


17514R-3.IR_full       361 ATTGAATGCTTCTAAAAGTCCCCTATGTGCCGAAGTTCTTACTGACCTGTACATATTTTT 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17514R-3.in silico     361 ATTGAATGCTTCTAAAAGTCCCCTATGTGCCGAAGTTCTTACTGACCTGTACATATTTTT 420


17514R-3.IR_full       421 GCATAATCTGTGCTTTACTTTCCAGCCAAAACTTGGACGTGCCATCGCATTAGCGACAAT 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17514R-3.in silico     421 GCATAATCTGTGCTTTACTTTCCAGCCAAAACTTGGACGTGCCATCGCATTAGCGACAAT 480


17514R-3.IR_full       481 CAAACTTCAAAGCCCGTCCT 500
                           ||||||||||||||||||||
17514R-3.in silico     481 CAAACTTCAAAGCCCGTCCT 500



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  7  NM_001015316.1  CG17514-PA.3 (CG17514), mRNA 
0   0  2  3  2  NM_136227.1  CG9338-RA (CG9338), mRNA 
0   0  1  5  2  NM_140282.2  CG6928-RB, transcript variant B (CG6928), mRNA 
0   0  1  5  2  NM_168486.1  CG6928-RA, transcript variant A (CG6928), mRNA 
0   0  1  3  14  NM_001014565.1  CG10491-RB, transcript variant B (vn), mRNA 
0   0  1  3  6  NM_079218.2  CG10491-RA, transcript variant A (vn), mRNA 
0   0  1  3  2  NM_170348.1  CG31055-RB, transcript variant B (CG31055), mRNA 
0   0  1  3  2  NM_170347.1  CG31055-RC, transcript variant C (CG31055), mRNA 
0   0  1  3  2  NM_170346.1  CG31055-RA, transcript variant A (CG31055), mRNA 
0   0  1  2  5  NM_079347.2  CG32146-RA, transcript variant A (dlp), mRNA 
0   0  1  2  5  NM_206353.1  CG32146-RB, transcript variant B (dlp), mRNA 
0   0  1  2  4  NM_139505.3  CG32484-RA (Sk2), mRNA 
0   0  1  2  3  NM_141280.2  CG14669-RA (CG14669), mRNA 
0   0  1  2  3  NM_140687.4  CG6664-RA, transcript variant A (CG6664), mRNA 
0   0  1  2  3  NM_168710.3  CG6664-RC, transcript variant C (CG6664), mRNA 
0   0  1  2  2  NM_140966.1  CG5262-RA (CG5262), mRNA 
0   0  1  2  2  NM_137947.2  CG3530-RB, transcript variant B (CG3530), mRNA 
0   0  1  2  2  NM_166603.1  CG3530-RA, transcript variant A (CG3530), mRNA 
0   0  1  2  2  NM_166601.1  CG10315-RB, transcript variant B (eIF2B-delta), mRNA 
0   0  1  2  2  NM_166602.1  CG10315-RC, transcript variant C (eIF2B-delta), mRNA 
0   0  1  2  2  NM_137946.2  CG10315-RA, transcript variant A (eIF2B-delta), mRNA 
0   0  0  6  5  NM_141922.1  CG4115-RA (CG4115), mRNA 
0   0  0  6  4  NM_165837.2  CG17835-RA, transcript variant A (inv), mRNA 
0   0  0  6  4  NM_165839.2  CG17835-RD, transcript variant D (inv), mRNA 
0   0  0  6  2  NM_079371.2  CG17943-RA (comm), mRNA 
0   0  0  5  12  NM_135978.1  CG12288-RA (CG12288), mRNA 
0   0  0  5  11  NM_130709.1  CG14271-RB (Gas8), mRNA 
0   0  0  5  9  NM_001031892.1  CG18350-RN, transcript variant N (Sxl), mRNA 
0   0  0  5  9  NM_167113.2  CG18350-RE, transcript variant E (Sxl), mRNA 
0   0  0  5  9  NM_167115.2  CG18350-RA, transcript variant A (Sxl), mRNA 
 Wing Disc
GFP
 Puc
 Caspase
DAPI
 Merge
 Figure
JNK signal
N
 
DB
 
DC
 
DM
 
VM
 
VB
 
Caspase-3 signal
N
 
DB
 
DC
 
DM
 
VM
 
VB
 
Dcompartment   Penetrance
     

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