NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 1746R-3

Symbol CG1746 Full Name CG1746

CG No CG1746 Old CG No CG1746

Synonyms anon-EST:Posey224, CG1746

Accession No (Link to NCBI) NM_143595.1

Inserted Chr. ll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
[Please submit your publication] Ohhara Y, Nakamura A, Kato Y, Yamakawa-Kobayashi K.
Chaperonin TRiC/CCT supports mitotic exit and entry into endocycle in Drosophila.
PLoS Genet (2019) 15(4) e1008121 [ PubMed ID = 31034473 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgTCGTGTCGACAGTCTCTCGCA

Primer Seq. 3'

aatctagaggtaccTTAGAAGGCGAACAGCAGCAG

Predicted Fragment Size

IR fragment full Seq

0001 TCGTGTCGAC AGTCTCTCGC ATTGCCCCAG TTGCCAGGAG CGCCTTCCTC GCCAACTCCA 
0061 AGCAGTACTT GCGACCATTG AGCAGCGCCA TCATCAGCCA GAGCCAGACT TTGGCCGCTC 
0121 AGAACACAAC CCCCGTTGCA TTGCTGCCAC AGATCAGGTC ATTCCAGACC TCGCCAGTCA 
0181 CGCGTGACAT TGACTCGGCC GCCAAATTCA TTGGTGCTGG TGCCGCAACA GTCGGTGTCG 
0241 CTGGATCCGG TGCTGGTATC GGAACAGTAT TCGGTTCCCT CATCATCGGC TACGCCAGGA 
0301 ACCCATCGCT GAAACAGCAG CTGTTCTCCT ACGCCATNTC TgGGCTTCGC CCTGTCCGAG 
0361 GCCATGGNGT CTGTTCTGTT TGATGATGGC CTTCCTGCTG CTGTTCGCCT TCTAA

in silico PCR Fragment

0001 TCGTGTCGAC AGTCTCTCGC ATTGCCCCAG TTGCCAGGAG CGCCTTCCTC GCCAACTCCA 
0061 AGCAGTACTT GCGACCATTG AGCAGCGCCA TCATCAGCCA GAGCCAGACT TTGGCCGCTC 
0121 AGAACACAAC CCCCGTTGCA TTGCTGCCAC AGATCAGGTC ATTCCAGACC TCGCCAGTCA 
0181 CGCGTGACAT TGACTCGGCC GCCAAATTCA TTGGTGCTGG TGCCGCAACA GTCGGTGTCG 
0241 CTGGATCCGG TGCTGGTATC GGAACAGTAT TCGGTTCCCT CATCATCGGC TACGCCAGGA 
0301 ACCCATCGCT GAAACAGCAG CTGTTCTCCT ACGCCAT-TC TGGGCTTCGC CCTGTCCGAG 
0361 GCCATGG-GT CTGTTCTGTT TGATGATGGC CTTCCTGCTG CTGTTCGCCT TCTAA

Assemble Data


1746R-3.IR_full       1   TCGTGTCGACAGTCTCTCGCATTGCCCCAGTTGCCAGGAGCGCCTTCCTCGCCAACTCCA 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1746R-3.in silico     1   TCGTGTCGACAGTCTCTCGCATTGCCCCAGTTGCCAGGAGCGCCTTCCTCGCCAACTCCA 60


1746R-3.IR_full       61  AGCAGTACTTGCGACCATTGAGCAGCGCCATCATCAGCCAGAGCCAGACTTTGGCCGCTC 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1746R-3.in silico     61  AGCAGTACTTGCGACCATTGAGCAGCGCCATCATCAGCCAGAGCCAGACTTTGGCCGCTC 120


1746R-3.IR_full       121 AGAACACAACCCCCGTTGCATTGCTGCCACAGATCAGGTCATTCCAGACCTCGCCAGTCA 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1746R-3.in silico     121 AGAACACAACCCCCGTTGCATTGCTGCCACAGATCAGGTCATTCCAGACCTCGCCAGTCA 180


1746R-3.IR_full       181 CGCGTGACATTGACTCGGCCGCCAAATTCATTGGTGCTGGTGCCGCAACAGTCGGTGTCG 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1746R-3.in silico     181 CGCGTGACATTGACTCGGCCGCCAAATTCATTGGTGCTGGTGCCGCAACAGTCGGTGTCG 240


1746R-3.IR_full       241 CTGGATCCGGTGCTGGTATCGGAACAGTATTCGGTTCCCTCATCATCGGCTACGCCAGGA 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1746R-3.in silico     241 CTGGATCCGGTGCTGGTATCGGAACAGTATTCGGTTCCCTCATCATCGGCTACGCCAGGA 300


1746R-3.IR_full       301 ACCCATCGCTGAAACAGCAGCTGTTCTCCTACGCCATNTCTGGGCTTCGCCCTGTCCGAG 360
                          ||||||||||||||||||||||||||||||||||||| ||||||||||||||||||||||
1746R-3.in silico     301 ACCCATCGCTGAAACAGCAGCTGTTCTCCTACGCCAT-TCTGGGCTTCGCCCTGTCCGAG 360


1746R-3.IR_full       361 GCCATGGNGTCTGTTCTGTTTGATGATGGCCTTCCTGCTGCTGTTCGCCTTCTAA 415
                          ||||||| |||||||||||||||||||||||||||||||||||||||||||||||
1746R-3.in silico     361 GCCATGG-GTCTGTTCTGTTTGATGATGGCCTTCCTGCTGCTGTTCGCCTTCTAA 415

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	395	0	0	4	NM_143595.1	CG1746-RA, transcript variant A (CG1746), mRNA
100	395	0	0	4	NM_170544.1	CG1746-RC, transcript variant C (CG1746), mRNA
100	395	0	0	4	NM_170543.1	CG1746-RB, transcript variant B (CG1746), mRNA
0.25	1	3	3	2	NM_170308.1	CG6134-RF, transcript variant F (spz), mRNA
0.25	1	3	3	2	NM_170312.1	CG6134-RB, transcript variant B (spz), mRNA
0.25	1	3	3	2	NM_170309.1	CG6134-RI, transcript variant I (spz), mRNA
0.25	1	3	3	2	NM_170311.1	CG6134-RH, transcript variant H (spz), mRNA
0.25	1	3	3	2	NM_170310.1	CG6134-RE, transcript variant E (spz), mRNA
0.25	1	3	3	2	NM_079802.2	CG6134-RA, transcript variant A (spz), mRNA
0.25	1	3	3	2	NM_170315.1	CG6134-RC, transcript variant C (spz), mRNA
0.25	1	3	3	2	NM_170313.1	CG6134-RJ, transcript variant J (spz), mRNA
0.25	1	3	3	2	NM_170314.1	CG6134-RG, transcript variant G (spz), mRNA
0.25	1	3	3	2	NM_170316.1	CG6134-RD, transcript variant D (spz), mRNA
0	0	6	3	2	NM_137697.2	CG9353-RA (mRpL54), mRNA
0	0	4	2	1	NM_169423.2	CG17342-RB, transcript variant B (Lk6), mRNA
0	0	3	2	4	NM_132018.2	CG3149-RA (CG3149), mRNA
0	0	2	2	3	NM_167893.1	CG1049-RC, transcript variant C (Cct1), mRNA
0	0	2	2	3	NM_167892.1	CG1049-RB, transcript variant B (Cct1), mRNA
0	0	2	2	3	NM_167894.1	CG1049-RD, transcript variant D (Cct1), mRNA
0	0	2	2	3	NM_139364.2	CG1049-RA, transcript variant A (Cct1), mRNA
0	0	2	2	2	NM_141262.2	CG12005-RB (Mms19), mRNA
0	0	1	6	4	NM_167789.1	CG1210-RC, transcript variant C (Pk61C), mRNA
0	0	1	6	4	NM_167791.1	CG1210-RF, transcript variant F (Pk61C), mRNA
0	0	1	6	4	NM_167792.1	CG1210-RH, transcript variant H (Pk61C), mRNA
0	0	1	6	4	NM_080382.2	CG1210-RA, transcript variant A (Pk61C), mRNA
0	0	1	6	4	NM_167790.1	CG1210-RD, transcript variant D (Pk61C), mRNA
0	0	1	5	4	NM_079181.2	CG1065-RA (Scsalpha), mRNA
0	0	1	5	3	NM_142365.1	CG5860-RA (CG5860), mRNA
0	0	1	5	2	NM_167794.1	CG1210-RE, transcript variant E (Pk61C), mRNA
0	0	1	5	2	NM_167793.1	CG1210-RB, transcript variant B (Pk61C), mRNA

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