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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 17387R-2 
 Symbol CG17387  Full Name CG17387 
 CG No CG17387  Old CG No CG17387 
 Synonyms CG17387 
 Accession No (Link to NCBI) NM_141238.1 
 Inserted Chr. lll 
 Insertional Mutation  lethal 
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgATTCCAATGGCGAGTGCCGAG 
 Primer Seq. 3' 
aatctagaggtaccGCCTGCAAGAGCTGACGCTTA 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 ATTCCAATGG CGAGTGCCGA GAACTTGGCC ATCCTAAAGG AACTGACTGC TCTGCGGGCG 
0061 TTGAAGCTGG AGCTGCAGCA GCACATGAAN CTCGCTGGAT GAGCGCGAGA TGGCGGTGGA 
0121 ACGACATACC CGCAACATTG AGGGCACTAT TCAGCAGAAC CTGACACTGT ACAGCTCCCT 
0181 GAAGGATGAT GTTATCAAGG AGGCGCACAA CGTCCAGCTT GTGATCTTGG AGCGCAACAA 
0241 GATCAAGGAG GATCTGCGGA AGAATAACAA GGAGCTGGAG GAGTACACTG AATATGTCAA 
0301 AGTCACTGAG CGAAAGATTT GCCCAGAGCA AGCGCGAGAT AGATGAGCTG ACTTCCCCGC 
0361 ATCAAGTCGG CTAAGACTAC CTTGGTGGAG TGGACAGAGG CCATGGAAGA CGGAAACAAG 
0421 GGCTACCAGC TAATCGAAAA GTACTACCTC GATGACCAGC AAAAGGCACG GGAGCTGAAC 
0481 ATTAAGCGTC AGCTCTTGCA GGC 
 in silico PCR Fragment 
0001 ATTCCAATGG CGAGTGCCGA GAACTTGGCC ATCCTAAAGG AACTGACTGC TCTGCGGGCG 
0061 TTGAAGCTGG AGCTGCAGCA GCACATGAA- CTCGCTGGAT GAGCGCGAGA TGGCGGTGGA 
0121 ACGACATACC CGCAACATTG AGGGCACTAT TCAGCAGAAC CTGACACTGT ACAGCTCCCT 
0181 GAAGGATGAT GTTATCAAGG AGGCGCACAA CGTCCAGCTT GTGATCTTGG AGCGCAACAA 
0241 GATCAAGGAG GATCTGCGGA AGAATAACAA GGAGCTGGAG GAGTACACTG AATATGTCAA 
0301 AGTCACTGAG CGAAAGATTT G-CCAGAGCA AGCGCGAGAT AGATGAGCTG ACTT-CCCGC 
0361 ATCAAGTCGG CTAAGACTAC CTTGGTGGAG TGGACAGAGG CCATGGAAGA CGGAAACAAG 
0421 GGCTACCAGC TAATCGAAAA GTACTACCTC GATGACCAGC AAAAGGCACG GGAGCTGAAC 
0481 ATTAAGCGTC AGCTCTTGCA GGC 
 Assemble Data 

17387R-2.IR_full       1   ATTCCAATGGCGAGTGCCGAGAACTTGGCCATCCTAAAGGAACTGACTGCTCTGCGGGCG 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17387R-2.in silico     1   ATTCCAATGGCGAGTGCCGAGAACTTGGCCATCCTAAAGGAACTGACTGCTCTGCGGGCG 60


17387R-2.IR_full       61  TTGAAGCTGGAGCTGCAGCAGCACATGAANCTCGCTGGATGAGCGCGAGATGGCGGTGGA 120
                           ||||||||||||||||||||||||||||| ||||||||||||||||||||||||||||||
17387R-2.in silico     61  TTGAAGCTGGAGCTGCAGCAGCACATGAA-CTCGCTGGATGAGCGCGAGATGGCGGTGGA 120


17387R-2.IR_full       121 ACGACATACCCGCAACATTGAGGGCACTATTCAGCAGAACCTGACACTGTACAGCTCCCT 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17387R-2.in silico     121 ACGACATACCCGCAACATTGAGGGCACTATTCAGCAGAACCTGACACTGTACAGCTCCCT 180


17387R-2.IR_full       181 GAAGGATGATGTTATCAAGGAGGCGCACAACGTCCAGCTTGTGATCTTGGAGCGCAACAA 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17387R-2.in silico     181 GAAGGATGATGTTATCAAGGAGGCGCACAACGTCCAGCTTGTGATCTTGGAGCGCAACAA 240


17387R-2.IR_full       241 GATCAAGGAGGATCTGCGGAAGAATAACAAGGAGCTGGAGGAGTACACTGAATATGTCAA 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17387R-2.in silico     241 GATCAAGGAGGATCTGCGGAAGAATAACAAGGAGCTGGAGGAGTACACTGAATATGTCAA 300


17387R-2.IR_full       301 AGTCACTGAGCGAAAGATTTGCCCAGAGCAAGCGCGAGATAGATGAGCTGACTTCCCCGC 360
                           ||||||||||||||||||||| |||||||||||||||||||||||||||||||| |||||
17387R-2.in silico     301 AGTCACTGAGCGAAAGATTTG-CCAGAGCAAGCGCGAGATAGATGAGCTGACTT-CCCGC 360


17387R-2.IR_full       361 ATCAAGTCGGCTAAGACTACCTTGGTGGAGTGGACAGAGGCCATGGAAGACGGAAACAAG 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17387R-2.in silico     361 ATCAAGTCGGCTAAGACTACCTTGGTGGAGTGGACAGAGGCCATGGAAGACGGAAACAAG 420


17387R-2.IR_full       421 GGCTACCAGCTAATCGAAAAGTACTACCTCGATGACCAGCAAAAGGCACGGGAGCTGAAC 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17387R-2.in silico     421 GGCTACCAGCTAATCGAAAAGTACTACCTCGATGACCAGCAAAAGGCACGGGAGCTGAAC 480


17387R-2.IR_full       481 ATTAAGCGTCAGCTCTTGCAGGC 503
                           |||||||||||||||||||||||
17387R-2.in silico     481 ATTAAGCGTCAGCTCTTGCAGGC 503



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  8  NM_141238.1  CG17387-RA (CG17387), mRNA 
0   0  3  4  2  NM_132537.1  CG15738-RA (CG15738), mRNA 
0   0  3  2  9  NM_170094.2  CG13598-RA, transcript variant A (sba), mRNA 
0   0  3  2  9  NM_176549.1  CG13598-RB, transcript variant B (sba), mRNA 
0   0  3  2  5  NM_139732.2  CG10542-RA (CG10542), mRNA 
0   0  2  6  5  NM_206382.1  CG5151-RB, transcript variant B (CG5151), mRNA 
0   0  2  3  4  NM_057728.3  CG18000-RC, transcript variant C (sw), mRNA 
0   0  2  3  4  NM_057731.3  CG18000-RF, transcript variant F (sw), mRNA 
0   0  2  3  4  NM_057721.3  CG18000-RD, transcript variant D (sw), mRNA 
0   0  2  3  4  NM_057729.3  CG18000-RB, transcript variant B (sw), mRNA 
0   0  2  3  4  NM_057727.4  CG18000-RJ, transcript variant J (sw), mRNA 
0   0  2  3  4  NM_057722.3  CG18000-RG, transcript variant G (sw), mRNA 
0   0  2  3  4  NM_057724.3  CG18000-RI, transcript variant I (sw), mRNA 
0   0  2  3  4  NM_167693.2  CG32823-RB (Sdic:CG32823), mRNA 
0   0  2  3  4  NM_057725.3  CG18000-RK, transcript variant K (sw), mRNA 
0   0  2  3  4  NM_057723.3  CG18000-RH, transcript variant H (sw), mRNA 
0   0  2  3  4  NM_057726.3  CG18000-RA, transcript variant A (sw), mRNA 
0   0  2  3  4  NM_057730.4  CG18000-RE, transcript variant E (sw), mRNA 
0   0  2  3  4  NM_206798.1  CG18000-RM, transcript variant M (sw), mRNA 
0   0  2  3  4  NM_206796.1  CG33499-RA (Sdic:CG33499), mRNA 
0   0  2  3  4  NM_206799.1  CG18000-RL, transcript variant L (sw), mRNA 
0   0  2  3  4  NM_140968.2  CG11796-RB, transcript variant B (CG11796), mRNA 
0   0  2  3  4  NM_168857.1  CG11796-RA, transcript variant A (CG11796), mRNA 
0   0  2  3  4  NM_079931.3  CG9580-RA (Sdic:CG9580), mRNA 
0   0  2  3  4  NM_206793.1  CG33497-RA (Sdic:CG33497), mRNA 
0   0  2  2  3  NM_143130.2  CG10675-RA (CHKov2), mRNA 
0   0  2  2  2  NM_167232.1  CG2890-RA, transcript variant A (PPP4R2r), mRNA 
0   0  2  2  2  NM_206677.1  CG2890-RC, transcript variant C (PPP4R2r), mRNA 
0   0  2  2  2  NM_080344.2  CG2890-RB, transcript variant B (PPP4R2r), mRNA 
0   0  1  9  2  NM_142710.2  CG16791-RA (CG16791), mRNA 

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