NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 17386R-2

Symbol CG17386 Full Name CG17386

CG No CG17386 Old CG No CG17386

Synonyms CT33484, CG17386

Accession No (Link to NCBI) NM_137120.1

Inserted Chr. ll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
[Please submit your publication] Ohhara Y, Hoshino G, Imahori K, Matsuyuki T, Yamakawa-Kobayashi K.
The Nutrient-Responsive Molecular Chaperone Hsp90 Supports Growth and Development in Drosophila.
Front Physiol (2021) 12 690564 [ PubMed ID = 34239451 ] [ RRC reference ]

Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624 [ PubMed ID = 21666802 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCGGAGCAGGTGTACGAGGTGA

Primer Seq. 3'

aatctagaggtaccGGTTAGGTCCACCTCGGCTAT

Predicted Fragment Size

IR fragment full Seq

0001 CGGAGNAGNT GTACGAGGTG AAGGCTCCAC ATGCCGGTGC AGAGGGTCAC ACGTTCCTTG 
0061 TGAGTCCCGT GGACCCCACT CCGATCCTAG CGCCCATTCT CACCGCTGTG CCTGTGATGA 
0121 AAGCACCGGA TTCGCCTGCT TCAATGGACN AAGACCAAGG AGATGGAGGA GCTGGATGAT 
0181 GAGCAGCCGA GCTGCAGNTT CCCAGGCGGT GGTGCCGTTG TCCTCATCGC TGCCCTGCAA 
0241 ANGCTGCTCC GCATGCATGC CAGACGCTCG GCCCAGGCGC TGCTCCACCA AATGGAATCG 
0301 ATGGACTCTC AGCTGGGCAG CAGCAATGGC AGCACCTCGG AGGATACTTC ACCCTCTGCT 
0361 CCGCCCATGT CGCCCACTGA CCACCAACTG GTGGATGTGG GCATGGGTTC CTCCATTGGG 
0421 GACTCGGATG AATCGTCGGA AGAGGGTGAT GAGCTGAAGC CCNTGGCCGT GGACAATCAC 
0481 ATCATAGCCG AGGTGGAcCT AACC

in silico PCR Fragment

0001 CGGAGCAGGT GTACGAGGTG AAGGCTCCAC ATGCCGGTGC AGAGGGTCAC ACGTTCCTTG 
0061 TGAGTCCCGT GGACCCCACT CCGATCCTAG CGCCCATTCT CACCGCTGTG CCTGTGATGA 
0121 AAGCACCGGA TTCGCCTGCT TCAATGGAC- AAGACCAAGG AGATGGAGGA GCTGGATGAT 
0181 GAGCAGCCGA GCTGCAG-TT CCCAGGCGGT GGTGCCGTTG TCCTCATCGC TGCCCTGCAA 
0241 --GCTGCTCC GCATGCATGC CAGACGCTCG GCCCAGGCGC TGCTCCACCA AATGGAATCG 
0301 ATGGACTCTC AGCTGGGCAG CAGCAATGGC AGCACCTCGG AGGATACTTC ACCCTCTGCT 
0361 CCGCCCATGT CGCCCACTGA CCACCAACTG GTGGATGTGG GCATGGGTTC CTCCATTGGG 
0421 GACTCGGATG AATCGTCGGA AGAGGGTGAT GAGCTGAAGC CCCTGGCCGT GGACAATCAC 
0481 ATCATAGCCG AGGTGGACCT AACC

Assemble Data


17386R-2.IR_full       1   CGGAGNAGNTGTACGAGGTGAAGGCTCCACATGCCGGTGCAGAGGGTCACACGTTCCTTG 60
                           ||||| || |||||||||||||||||||||||||||||||||||||||||||||||||||
17386R-2.in silico     1   CGGAGCAGGTGTACGAGGTGAAGGCTCCACATGCCGGTGCAGAGGGTCACACGTTCCTTG 60


17386R-2.IR_full       61  TGAGTCCCGTGGACCCCACTCCGATCCTAGCGCCCATTCTCACCGCTGTGCCTGTGATGA 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17386R-2.in silico     61  TGAGTCCCGTGGACCCCACTCCGATCCTAGCGCCCATTCTCACCGCTGTGCCTGTGATGA 120


17386R-2.IR_full       121 AAGCACCGGATTCGCCTGCTTCAATGGACNAAGACCAAGGAGATGGAGGAGCTGGATGAT 180
                           ||||||||||||||||||||||||||||| ||||||||||||||||||||||||||||||
17386R-2.in silico     121 AAGCACCGGATTCGCCTGCTTCAATGGAC-AAGACCAAGGAGATGGAGGAGCTGGATGAT 180


17386R-2.IR_full       181 GAGCAGCCGAGCTGCAGNTTCCCAGGCGGTGGTGCCGTTGTCCTCATCGCTGCCCTGCAA 240
                           ||||||||||||||||| ||||||||||||||||||||||||||||||||||||||||||
17386R-2.in silico     181 GAGCAGCCGAGCTGCAG-TTCCCAGGCGGTGGTGCCGTTGTCCTCATCGCTGCCCTGCAA 240


17386R-2.IR_full       241 ANGCTGCTCCGCATGCATGCCAGACGCTCGGCCCAGGCGCTGCTCCACCAAATGGAATCG 300
                             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17386R-2.in silico     241 --GCTGCTCCGCATGCATGCCAGACGCTCGGCCCAGGCGCTGCTCCACCAAATGGAATCG 300


17386R-2.IR_full       301 ATGGACTCTCAGCTGGGCAGCAGCAATGGCAGCACCTCGGAGGATACTTCACCCTCTGCT 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17386R-2.in silico     301 ATGGACTCTCAGCTGGGCAGCAGCAATGGCAGCACCTCGGAGGATACTTCACCCTCTGCT 360


17386R-2.IR_full       361 CCGCCCATGTCGCCCACTGACCACCAACTGGTGGATGTGGGCATGGGTTCCTCCATTGGG 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17386R-2.in silico     361 CCGCCCATGTCGCCCACTGACCACCAACTGGTGGATGTGGGCATGGGTTCCTCCATTGGG 420


17386R-2.IR_full       421 GACTCGGATGAATCGTCGGAAGAGGGTGATGAGCTGAAGCCCNTGGCCGTGGACAATCAC 480
                           |||||||||||||||||||||||||||||||||||||||||| |||||||||||||||||
17386R-2.in silico     421 GACTCGGATGAATCGTCGGAAGAGGGTGATGAGCTGAAGCCCCTGGCCGTGGACAATCAC 480


17386R-2.IR_full       481 ATCATAGCCGAGGTGGACCTAACC 504
                           ||||||||||||||||||||||||
17386R-2.in silico     481 ATCATAGCCGAGGTGGACCTAACC 504

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_137120.1	CG17386-RA (CG17386), mRNA
0.41	2	3	2	2	NM_080027.4	CG12052-RG, transcript variant G (lola), mRNA
0.41	2	3	2	2	NM_176132.2	CG12052-RR, transcript variant R (lola), mRNA
0.41	2	3	2	2	NM_079711.2	CG6545-RA (lbe), mRNA
0.41	2	2	2	3	NM_140199.2	CG7590-RA, transcript variant A (scyl), mRNA
0.41	2	2	2	3	NM_206321.1	CG7590-RB, transcript variant B (scyl), mRNA
0.41	2	2	2	2	NM_079710.2	CG6570-RA (lbl), mRNA
0	0	7	3	6	NM_164386.2	CG11822-RB, transcript variant B (nAcRbeta-21C), mRNA
0	0	7	3	6	NM_080359.3	CG11822-RA, transcript variant A (nAcRbeta-21C), mRNA
0	0	7	3	3	NM_143092.2	CG10244-RA (Cad96Ca), mRNA
0	0	4	4	3	NM_139833.1	CG8616-RA (CG8616), mRNA
0	0	3	6	6	NM_132858.2	CG9056-RA (CG9056), mRNA
0	0	3	5	9	NM_169826.1	CG31224-RA (CG31224), mRNA
0	0	3	3	5	NM_137128.2	CG12864-RA, transcript variant A (Su(var)2-HP2), mRNA
0	0	3	3	4	NM_176174.1	CG12864-RB, transcript variant B (Su(var)2-HP2), mRNA
0	0	2	6	15	NM_170420.1	CG18741-RA, transcript variant A (DopR2), mRNA
0	0	2	6	15	NM_079824.2	CG18741-RB, transcript variant B (DopR2), mRNA
0	0	2	3	7	NM_079118.2	CG3401-RA (betaTub60D), mRNA
0	0	2	3	4	NM_168495.1	CG32100-RA (CG32100), mRNA
0	0	2	3	4	NM_169202.1	CG11094-RA, transcript variant A (dsx), mRNA
0	0	2	3	3	NM_140300.2	CG5632-RA (thoc6), mRNA
0	0	2	3	2	NM_134515.1	CG11942-RA (skpE), mRNA
0	0	2	2	11	NM_130616.2	CG4290-RA (CG4290), mRNA
0	0	2	2	4	NM_168571.2	CG32133-RA (CG32133), mRNA
0	0	2	2	2	NM_079703.2	CG3593-RA (r-l), mRNA
0	0	1	8	2	NM_170639.1	CG17122-RA (CG17122), mRNA
0	0	1	7	3	NM_057805.2	CG9660-RA, transcript variant A (toc), mRNA
0	0	1	6	11	NM_167309.1	CG32663-RA (CG32663), mRNA
0	0	1	5	11	NM_080025.1	CG11387-RA, transcript variant A (ct), mRNA
0	0	1	5	7	NM_079471.2	CG18023-RA, transcript variant A (Eip78C), mRNA

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