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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 17122R-1 
 Symbol CG17122  Full Name CG17122 
 CG No CG17122  Old CG No CG17122 
 Synonyms CG17122 
 Accession No (Link to NCBI) NM_170639.1 
 Inserted Chr. ll 
 Insertional Mutation  lethal 
 Phenotype induced by Act5C-GAL4 at 28 degrees pupal lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgGATGTGAACAAGGTGACCCAG 
 Primer Seq. 3' 
aatctagaggtaccTCCTTCTCCACCACAGCTCGT 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 GATGTGAACA AGGTGACCCA GGTGGACAGA ACCTTCTACG AGCTGACCAT CACGGATCTC 
0061 AACCAGAAAC TAGCTCGACT GCGATCCCAC GTGACCACCG TCGATGAGTC CAACATCACG 
0121 CTGACCGAGA AACTCAAGGA AGTGGAGGCC GATGGCGTCG ACGTGGCCGC TCACTTGGAA 
0181 CGCACCCTGG CGGAGAGGAA TAACTCGATC ACCGAACTGG AGGAGCGCCT GGTAGAGATC 
0241 ACCAAGGTGC GGGATGTGGA GAACCGCGTG GCACAGGAGA AGATCGGCGA CTTGGAGTCG 
0301 AAATACAAGG CCATGCACGA CCAGTTGACC TCCGAAATAA AGCTGCTTAA TGGCAAGCTG 
0361 AATTCCCTCG ACGAGTTCCG CATCCAGAGG GATGTCCTGT TGGCCAAGTT CGATGAGCAG 
0421 GAGGCGGATC TCAATGAGCG CGAGAAGGAT CACAAGGAGG CGCTCTATAA TATGGAACAA 
0481 CGAGCTGTGG TGGAGAAGGA  
 in silico PCR Fragment 
0001 GATGTGAACA AGGTGACCCA GGTGGACAGA ACCTTCTACG AGCTGACCAT CACGGATCTC 
0061 AACCAGAAAC TAGCTCGACT GCGATCCCAC GTGACCACCG TCGATGAGTC CAACATCACG 
0121 CTGACCGAGA AACTCAAGGA AGTGGAGGCC GATGGCGTCG ACGTGGCCGC TCACTTGGAA 
0181 CGCACCCTGG CGGAGAGGAA TAACTCGATC ACCGAACTGG AGGAGCGCCT GGTAGAGATC 
0241 ACCAAGGTGC GGGATGTGGA GAACCGCGTG GCACAGGAGA AGATCGGCGA CTTGGAGTCG 
0301 AAATACAAGG CCATGCACGA CCAGTTGACC TCCGAAATAA AGCTGCTTAA TGGCAAGCTG 
0361 AATTCCCTCG ACGAGTTCCG CATCCAGAGG GATGTCCTGT TGGCCAAGTT CGATGAGCAG 
0421 GAGGCGGATC TCAATGAGCG CGAGAAGGAT CACAAGGAGG CGCTCTATAA TATGGAACAA 
0481 CGAGCTGTGG TGGAGAAGGA  
 Assemble Data 

17122R-1.IR_full       1   GATGTGAACAAGGTGACCCAGGTGGACAGAACCTTCTACGAGCTGACCATCACGGATCTC 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17122R-1.in silico     1   GATGTGAACAAGGTGACCCAGGTGGACAGAACCTTCTACGAGCTGACCATCACGGATCTC 60


17122R-1.IR_full       61  AACCAGAAACTAGCTCGACTGCGATCCCACGTGACCACCGTCGATGAGTCCAACATCACG 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17122R-1.in silico     61  AACCAGAAACTAGCTCGACTGCGATCCCACGTGACCACCGTCGATGAGTCCAACATCACG 120


17122R-1.IR_full       121 CTGACCGAGAAACTCAAGGAAGTGGAGGCCGATGGCGTCGACGTGGCCGCTCACTTGGAA 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17122R-1.in silico     121 CTGACCGAGAAACTCAAGGAAGTGGAGGCCGATGGCGTCGACGTGGCCGCTCACTTGGAA 180


17122R-1.IR_full       181 CGCACCCTGGCGGAGAGGAATAACTCGATCACCGAACTGGAGGAGCGCCTGGTAGAGATC 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17122R-1.in silico     181 CGCACCCTGGCGGAGAGGAATAACTCGATCACCGAACTGGAGGAGCGCCTGGTAGAGATC 240


17122R-1.IR_full       241 ACCAAGGTGCGGGATGTGGAGAACCGCGTGGCACAGGAGAAGATCGGCGACTTGGAGTCG 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17122R-1.in silico     241 ACCAAGGTGCGGGATGTGGAGAACCGCGTGGCACAGGAGAAGATCGGCGACTTGGAGTCG 300


17122R-1.IR_full       301 AAATACAAGGCCATGCACGACCAGTTGACCTCCGAAATAAAGCTGCTTAATGGCAAGCTG 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17122R-1.in silico     301 AAATACAAGGCCATGCACGACCAGTTGACCTCCGAAATAAAGCTGCTTAATGGCAAGCTG 360


17122R-1.IR_full       361 AATTCCCTCGACGAGTTCCGCATCCAGAGGGATGTCCTGTTGGCCAAGTTCGATGAGCAG 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17122R-1.in silico     361 AATTCCCTCGACGAGTTCCGCATCCAGAGGGATGTCCTGTTGGCCAAGTTCGATGAGCAG 420


17122R-1.IR_full       421 GAGGCGGATCTCAATGAGCGCGAGAAGGATCACAAGGAGGCGCTCTATAATATGGAACAA 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17122R-1.in silico     421 GAGGCGGATCTCAATGAGCGCGAGAAGGATCACAAGGAGGCGCTCTATAATATGGAACAA 480


17122R-1.IR_full       481 CGAGCTGTGGTGGAGAAGGA 500
                           ||||||||||||||||||||
17122R-1.in silico     481 CGAGCTGTGGTGGAGAAGGA 500



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  1  NM_170639.1  CG17122-RA (CG17122), mRNA 
0.41   2  4  2  3  NM_078854.1  CG18096-RA (TepI), mRNA 
0.41   2  2  2  2  NM_140751.2  CG32190-RA (NUCB1), mRNA 
0.2   1  2  3  12  NM_001014668.1  CG8318-RD, transcript variant D (Nf1), mRNA 
0.2   1  2  3  12  NM_170252.2  CG8318-RC, transcript variant C (Nf1), mRNA 
0.2   1  2  3  12  NM_170253.2  CG8318-RB, transcript variant B (Nf1), mRNA 
0   0  4  2  4  NM_144305.2  CG18661-RA (CG18661), mRNA 
0   0  3  7  7  NM_080535.2  CG9774-RA (rok), mRNA 
0   0  2  5  9  NM_079913.2  CG8110-RA, transcript variant A (syd), mRNA 
0   0  2  5  9  NM_168245.2  CG8110-RB, transcript variant B (syd), mRNA 
0   0  2  4  3  NM_136201.3  CG2614-RA (CG2614), mRNA 
0   0  2  3  7  NM_137075.2  CG18568-RA (CG18568), mRNA 
0   0  2  2  5  NM_137384.2  CG4878-RB, transcript variant B (eIF3-S9), mRNA 
0   0  2  2  5  NM_166239.2  CG4878-RA, transcript variant A (eIF3-S9), mRNA 
0   0  2  2  4  NM_001032402.1  CG33957-RB, transcript variant B (cp309), mRNA 
0   0  2  2  4  NM_001032401.1  CG33957-RC, transcript variant C (cp309), mRNA 
0   0  2  2  2  NM_057910.3  CG4472-RA (Idgf1), mRNA 
0   0  1  4  2  NM_141250.1  CG14661-RA (CG14661), mRNA 
0   0  1  3  8  NM_057469.3  CG10718-RA (neb), mRNA 
0   0  1  3  6  NM_141858.1  CG17360-RA (CG17360), mRNA 
0   0  1  3  5  NM_168290.1  CG5939-RB, transcript variant B (Prm), mRNA 
0   0  1  3  5  NM_079258.2  CG5939-RA, transcript variant A (Prm), mRNA 
0   0  1  2  8  NM_166876.1  CG14622-RB, transcript variant B (DAAM), mRNA 
0   0  1  2  8  NM_166875.1  CG14622-RC, transcript variant C (DAAM), mRNA 
0   0  1  2  8  NM_130544.3  CG14622-RA, transcript variant A (DAAM), mRNA 
0   0  1  2  7  NM_058071.3  CG7885-RA, transcript variant A (RpII33), mRNA 
0   0  1  2  7  NM_165064.1  CG7885-RB, transcript variant B (RpII33), mRNA 
0   0  1  2  6  NM_132792.2  CG6227-RA (CG6227), mRNA 
0   0  1  2  6  NM_143367.2  CG10011-RA (CG10011), mRNA 
0   0  1  2  5  NM_143314.2  CG5634-RA (dsd), mRNA 
 Wing Disc
GFP
 Puc
 Caspase
DAPI
 Merge
 Figure
JNK signal
N
 
DB
 
DC
+- 
DM
 
VM
 
VB
 
Caspase-3 signal
N
++ 
DB
+ 
DC
+ 
DM
 
VM
 
VB
 
Dcompartment   Penetrance
     

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