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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 17119R-1 
 Symbol CG17119  Full Name CG17119 
 CG No CG17119  Old CG No CG17119 
 Synonyms CG17119 
 Accession No (Link to NCBI) NM_142859.2 
 Inserted Chr. ll 
 Insertional Mutation  lethal 
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgCTCCTCCTCCTGGCCACAGTT 
 Primer Seq. 3' 
aatctagaggtaccCTGTACAGGGTGAAGCCCACG 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 NTCCTCCTCC TGGCCACAGT TGCCCTGGGA AATGCCCAAA GCAGTCAGCT CACCGTCGAT 
0061 TCCCATGACA TCACCGTTCT GCTGAACAGC AACGAGACTT TTCTGGTGTT CGCCAACGGA 
0121 TNTGCTAGAC AGCGACGTGG AAGTTGCGCT GGGAACAGAT TCGGAGGATC ATTTGCTCCT 
0181 CGATCCCGCA ACGTTTGTGT ATCCAGCGGG CAGTACTCGA AATCAGTCGG TGGTGATAAC 
0241 TGGCCTCAAA GCCGGCAACG TCAAAGTGGT CGCAGATAGC GATGATGCGA ACAAAGAGAT 
0301 TGTGAAGGAT GTGTTCGTAC GCGTGACTGT GGCCAAATCG AGAGCTTTGA TCTACACCTC 
0361 CATCATCTTT GGCTGGGTTT ACTTTGTGGC CTGGTCGGTG TCCTTCTATC CGCAGATCTG 
0421 GAGCAACTAT CGCCGCAAGT CCGTCGAGGG ACTGAACTTT GATTTCCTGG CCCTCAATAT 
0481 CGTGGGCTTC ACCCTGTACA G 
 in silico PCR Fragment 
0001 CTCCTCCTCC TGGCCACAGT TGCCCTGGGA AATGCCCAAA GCAGTCAGCT CACCGTCGAT 
0061 TCCCATGACA TCACCGTTCT GCTGAACAGC AACGAGACTT TTCTGGTGTT CGCCAACGGA 
0121 T-TGCTAGAC AGCGACGTGG AAGTTGCGCT GGGAACAGAT TCGGAGGATC ATTTGCTCCT 
0181 CGATCCCGCA ACGTTTGTGT ATCCAGCGGG CAGTACTCGA AATCAGTCGG TGGTGATAAC 
0241 TGGCCTCAAA GCCGGCAACG TCAAAGTGGT CGCAGATAGC GATGATGCGA ACAAAGAGAT 
0301 TGTGAAGGAT GTGTTCGTAC GCGTGACTGT GGCCAAATCG AGAGCTTTGA TCTACACCTC 
0361 CATCATCTTT GGCTGGGTTT ACTTTGTGGC CTGGTCGGTG TCCTTCTATC CGCAGATCTG 
0421 GAGCAACTAT CGCCGCAAGT CCGTCGAGGG ACTGAACTTT GATTTCCTGG CCCTCAATAT 
0481 CGTGGGCTTC ACCCTGTACA G 
 Assemble Data 

17119R-1.IR_full        1   TCCTCCTCCTGGCCACAGTTGCCCTGGGAAATGCCCAAAGCAGTCAGCTCACCGTCGAT 59
                            |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17119R-1.in silico      1   TCCTCCTCCTGGCCACAGTTGCCCTGGGAAATGCCCAAAGCAGTCAGCTCACCGTCGAT 59


17119R-1.IR_full       61  TCCCATGACATCACCGTTCTGCTGAACAGCAACGAGACTTTTCTGGTGTTCGCCAACGGA 119
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17119R-1.in silico     61  TCCCATGACATCACCGTTCTGCTGAACAGCAACGAGACTTTTCTGGTGTTCGCCAACGGA 119


17119R-1.IR_full       121 TNTGCTAGACAGCGACGTGGAAGTTGCGCTGGGAACAGATTCGGAGGATCATTTGCTCCT 179
                           | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17119R-1.in silico     121 T-TGCTAGACAGCGACGTGGAAGTTGCGCTGGGAACAGATTCGGAGGATCATTTGCTCCT 179


17119R-1.IR_full       181 CGATCCCGCAACGTTTGTGTATCCAGCGGGCAGTACTCGAAATCAGTCGGTGGTGATAAC 239
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17119R-1.in silico     181 CGATCCCGCAACGTTTGTGTATCCAGCGGGCAGTACTCGAAATCAGTCGGTGGTGATAAC 239


17119R-1.IR_full       241 TGGCCTCAAAGCCGGCAACGTCAAAGTGGTCGCAGATAGCGATGATGCGAACAAAGAGAT 299
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17119R-1.in silico     241 TGGCCTCAAAGCCGGCAACGTCAAAGTGGTCGCAGATAGCGATGATGCGAACAAAGAGAT 299


17119R-1.IR_full       301 TGTGAAGGATGTGTTCGTACGCGTGACTGTGGCCAAATCGAGAGCTTTGATCTACACCTC 359
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17119R-1.in silico     301 TGTGAAGGATGTGTTCGTACGCGTGACTGTGGCCAAATCGAGAGCTTTGATCTACACCTC 359


17119R-1.IR_full       361 CATCATCTTTGGCTGGGTTTACTTTGTGGCCTGGTCGGTGTCCTTCTATCCGCAGATCTG 419
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17119R-1.in silico     361 CATCATCTTTGGCTGGGTTTACTTTGTGGCCTGGTCGGTGTCCTTCTATCCGCAGATCTG 419


17119R-1.IR_full       421 GAGCAACTATCGCCGCAAGTCCGTCGAGGGACTGAACTTTGATTTCCTGGCCCTCAATAT 479
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17119R-1.in silico     421 GAGCAACTATCGCCGCAAGTCCGTCGAGGGACTGAACTTTGATTTCCTGGCCCTCAATAT 479


17119R-1.IR_full       481 CGTGGGCTTCACCCTGTACAG 500
                           |||||||||||||||||||||
17119R-1.in silico     481 CGTGGGCTTCACCCTGTACAG 500



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  1  NM_176543.1  CG17119-RB, transcript variant B (CG17119), mRNA 
100   482  0  0  1  NM_142859.2  CG17119-RA, transcript variant A (CG17119), mRNA 
0   0  6  2  3  NM_137926.2  CG3092-RA (CG3092), mRNA 
0   0  3  3  2  NM_142229.2  CG4525-RA (CG4525), mRNA 
0   0  2  2  6  NM_142696.1  CG7000-RA (CG7000), mRNA 
0   0  2  2  4  NM_079984.2  CG7400-RA, transcript variant A (Fatp), mRNA 
0   0  2  2  4  NM_164934.1  CG7400-RB, transcript variant B (Fatp), mRNA 
0   0  2  2  3  NM_079470.2  CG10564-RA (Ac78C), mRNA 
0   0  1  10  4  NM_139813.2  CG17744-RA (CG17744), mRNA 
0   0  1  5  1  NM_142401.2  CG17806-RA (CG17806), mRNA 
0   0  1  3  2  NM_141808.3  CG17216-RA (KP78b), mRNA 
0   0  1  1  4  NM_142114.1  CG14850-RA (CG14850), mRNA 
0   0  0  5  7  NM_168402.1  CG32055-RA (CG32055), mRNA 
0   0  0  4  18  NM_166790.2  CG32019-RA, transcript variant A (bt), mRNA 
0   0  0  4  18  NM_205877.1  CG32019-RD, transcript variant D (bt), mRNA 
0   0  0  4  18  NM_205876.1  CG32019-RC, transcript variant C (bt), mRNA 
0   0  0  4  18  NM_205875.1  CG32019-RE, transcript variant E (bt), mRNA 
0   0  0  4  6  NM_141300.1  CG2919-RA (CG2919), mRNA 
0   0  0  4  4  NM_143348.2  CG4849-RA (CG4849), mRNA 
0   0  0  4  4  NM_170363.1  CG31054-RA (CG31054), mRNA 
0   0  0  4  4  NM_135227.1  CG11322-RA (CG11322), mRNA 
0   0  0  4  4  NM_140649.2  CG17286-RA (CG17286), mRNA 
0   0  0  4  4  NM_137197.1  CG12960-RA (CG12960), mRNA 
0   0  0  3  9  NM_167095.1  CG32743-RA (Smg1), mRNA 
0   0  0  3  7  NM_141611.1  CG9746-RA (CG9746), mRNA 
0   0  0  3  5  NM_057380.2  CG8355-RA, transcript variant A (sli), mRNA 
0   0  0  3  5  NM_057381.2  CG8355-RB, transcript variant B (sli), mRNA 
0   0  0  3  5  NM_057379.2  CG8355-RC, transcript variant C (sli), mRNA 
0   0  0  3  4  NM_137719.2  CG4302-RA (CG4302), mRNA 
0   0  0  3  4  NM_164655.2  CG7235-RB, transcript variant B (Hsp60C), mRNA 
 Wing Disc
GFP
 Puc
 Caspase
DAPI
 Merge
 Figure
JNK signal
N
 
DB
 
DC
 
DM
 
VM
 
VB
 
Caspase-3 signal
N
 
DB
 
DC
 
DM
 
VM
 
VB
 
Dcompartment   Penetrance
     

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