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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 17100R-3 
 Symbol CG17100  Full Name CG17100 
 CG No CG17100  Old CG No CG17100 
 Synonyms AAF24476.1, GM05287, unnamed, CG17100 
 Accession No (Link to NCBI) NM_080036.1 
 Inserted Chr. ll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Nitta Y, Yamazaki D, Sugie A, Hiroi M, Tabata T.
DISCO Interacting Protein 2 regulates axonal bifurcation and guidance of Drosophila mushroom body neurons.
Dev Biol (2017) 421(2) 233-244 [ PubMed ID = 27908785 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgCATCGAGATGGCCATCAGGCA 
 Primer Seq. 3' 
aatctagaggtaccTGGTCAGCGGGACCTTGGAAT 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 CATCGAGATG GCCATCAGGc ACCTGAAGCA CCTGCAGAGC GAGTGCCAGC AGAAGGAGAG 
0061 CGACTACCGG AGTGGCTACA TGGACTGTAT GAAGGAGGCG GCCAAGTTTC TCTACGACGT 
0121 TCACATGCAG GACTTTTGCC ACCGACTATT GGGTCGTCTG CAGGAGCACA TCGATGAGAT 
0181 GTTCAAAACG GACTGCTACA AGTCGNACGC GCAGCTGCCN ACATGCCGGA TAATGTAAGC 
0241 GCCTCCAGCG GCAGTCCCCA TCAGGCGTAC CACCCACCGC TGTGCCACCT GCGTGACATG 
0301 TTGGCCACCT CCGCCTCGGA TGTGGAGCAC AGTCAGGACC ATAACGACGT CAAGGATCTG 
0361 AGTTTCCGGA ACCATCTCAA CCAGCTTCAG AGGAGTCAGC AggcgGCGGN NGNCGCAGCA 
0421 GTAGcNGCAG CTGCCGTTGC AGTCgNCAAT GGCAGTTCGc CGGCTTCGAA TGCCGGTGTG 
0481 GATTCCAAGG TCNCGCTGAC CA 
 in silico PCR Fragment 
0001 CATCGAGATG GCCATCAGGC ACCTGAAGCA CCTGCAGAGC GAGTGCCAGC AGAAGGAGAG 
0061 CGACTACCGG AGTGGCTACA TGGACTGTAT GAAGGAGGCG GCCAAGTTCC TCTACGACGT 
0121 TCACATGCAG GACTTTTGCC ACCGACTATT GGGTCGCCTG CAGGAGCACA TCGATGAGAT 
0181 GTTCAAAACG GACTGCTACA AGTCG-ACGC GCAGCTGCC- ACATGCCGGA TAATGTAAGC 
0241 GCCTCCAGCG GCAGTCCCCA TCAGGCATAC CACCCACCGC TGTGCCACCT GCGTGACATG 
0301 TTGGCCACCT CCGCCTCGGA TGTGGAGCAC AGTCAGGACC ATAACGACGT CAAGGATCTG 
0361 AGTTTCCGGA ACCATCTCAA CCAGCTTCAG AGGAGTCAGC AGGCGGCGGC AGCCGCAGCA 
0421 GTAGCGGCAG CTGCCGTTGC AGTCGCCAAT GGCAGTTCGC CGGCTTCGAA TGCCGGTGTG 
0481 GATTCCAAGG TCCCGCTGAC CA 
 Assemble Data 

17100R-3.IR_full       1   CATCGAGATGGCCATCAGGCACCTGAAGCACCTGCAGAGCGAGTGCCAGCAGAAGGAGAG 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17100R-3.in silico     1   CATCGAGATGGCCATCAGGCACCTGAAGCACCTGCAGAGCGAGTGCCAGCAGAAGGAGAG 60


17100R-3.IR_full       61  CGACTACCGGAGTGGCTACATGGACTGTATGAAGGAGGCGGCCAAGTTTCTCTACGACGT 120
                           |||||||||||||||||||||||||||||||||||||||||||||||| |||||||||||
17100R-3.in silico     61  CGACTACCGGAGTGGCTACATGGACTGTATGAAGGAGGCGGCCAAGTTCCTCTACGACGT 120


17100R-3.IR_full       121 TCACATGCAGGACTTTTGCCACCGACTATTGGGTCGTCTGCAGGAGCACATCGATGAGAT 180
                           |||||||||||||||||||||||||||||||||||| |||||||||||||||||||||||
17100R-3.in silico     121 TCACATGCAGGACTTTTGCCACCGACTATTGGGTCGCCTGCAGGAGCACATCGATGAGAT 180


17100R-3.IR_full       181 GTTCAAAACGGACTGCTACAAGTCGNACGCGCAGCTGCCNACATGCCGGATAATGTAAGC 240
                           ||||||||||||||||||||||||| ||||||||||||| ||||||||||||||||||||
17100R-3.in silico     181 GTTCAAAACGGACTGCTACAAGTCG-ACGCGCAGCTGCC-ACATGCCGGATAATGTAAGC 240


17100R-3.IR_full       241 GCCTCCAGCGGCAGTCCCCATCAGGCGTACCACCCACCGCTGTGCCACCTGCGTGACATG 300
                           |||||||||||||||||||||||||| |||||||||||||||||||||||||||||||||
17100R-3.in silico     241 GCCTCCAGCGGCAGTCCCCATCAGGCATACCACCCACCGCTGTGCCACCTGCGTGACATG 300


17100R-3.IR_full       301 TTGGCCACCTCCGCCTCGGATGTGGAGCACAGTCAGGACCATAACGACGTCAAGGATCTG 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17100R-3.in silico     301 TTGGCCACCTCCGCCTCGGATGTGGAGCACAGTCAGGACCATAACGACGTCAAGGATCTG 360


17100R-3.IR_full       361 AGTTTCCGGAACCATCTCAACCAGCTTCAGAGGAGTCAGCAGGCGGCGGNNGNCGCAGCA 420
                           |||||||||||||||||||||||||||||||||||||||||||||||||  | |||||||
17100R-3.in silico     361 AGTTTCCGGAACCATCTCAACCAGCTTCAGAGGAGTCAGCAGGCGGCGGCAGCCGCAGCA 420


17100R-3.IR_full       421 GTAGCNGCAGCTGCCGTTGCAGTCGNCAATGGCAGTTCGCCGGCTTCGAATGCCGGTGTG 480
                           ||||| ||||||||||||||||||| ||||||||||||||||||||||||||||||||||
17100R-3.in silico     421 GTAGCGGCAGCTGCCGTTGCAGTCGCCAATGGCAGTTCGCCGGCTTCGAATGCCGGTGTG 480


17100R-3.IR_full       481 GATTCCAAGGTCNCGCTGACCA 502
                           |||||||||||| |||||||||
17100R-3.in silico     481 GATTCCAAGGTCCCGCTGACCA 502



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  6  NM_080036.1  CG17100-RA (CG17100), mRNA 
0   0  8  11  81  NM_132246.2  CG10555-RA (CG10555), mRNA 
0   0  5  8  26  NM_079914.2  CG9936-RD, transcript variant D (skd), mRNA 
0   0  5  8  26  NM_168879.2  CG9936-RC, transcript variant C (skd), mRNA 
0   0  5  8  19  NM_001043256.1  CG34157-RB, transcript variant B (Dys), mRNA 
0   0  5  5  15  NM_206684.2  CG1725-RE, transcript variant E (dlg1), mRNA 
0   0  5  5  15  NM_206682.2  CG1725-RG, transcript variant G (dlg1), mRNA 
0   0  5  5  15  NM_078565.3  CG1725-RD, transcript variant D (dlg1), mRNA 
0   0  5  5  15  NM_167282.2  CG1725-RA, transcript variant A (dlg1), mRNA 
0   0  5  5  13  NM_142456.1  CG15803-RA (CG15803), mRNA 
0   0  4  5  5  NM_169787.1  CG7913-RD, transcript variant D (PP2A-B'), mRNA 
0   0  4  5  5  NM_142424.2  CG7913-RE, transcript variant E (PP2A-B'), mRNA 
0   0  3  19  58  NM_169696.1  CG3992-RA, transcript variant A (srp), mRNA 
0   0  3  19  57  NM_169694.1  CG3992-RB, transcript variant B (srp), mRNA 
0   0  3  8  12  NM_080294.3  CG3992-RB, transcript variant B (srp), mRNA, abnormal vision CG4262-RA, transcript variant A (elav), mRNA 
0   0  3  8  12  NM_001014713.1  CG3992-RB, transcript variant B (srp), mRNA, abnormal vision CG4262-RA, transcript variant A (elav), mRNA, abnormal vision CG4262-RB, transcript variant B (elav), mRNA 
0   0  3  6  17  NM_132953.1  CG13001-RA (CG13001), mRNA 
0   0  3  2  10  NM_136854.1  CG13194-RA (pyr), mRNA 
0   0  3  2  3  NM_001043111.1  CG17046-RB, transcript variant B (klar), mRNA 
0   0  3  2  3  NM_079149.3  CG17046-RA, transcript variant A (klar), mRNA 
0   0  2  14  55  NM_143745.2  CG18389-RA (Eip93F), mRNA 
0   0  2  12  113  NM_134516.2  CG32529-RA, transcript variant A (CG32529), mRNA 
0   0  2  10  37  NM_001042815.1  CG9907-RB, transcript variant B (para), mRNA 
0   0  2  10  37  NM_001042816.1  CG9907-RC, transcript variant C (para), mRNA 
0   0  2  10  37  NM_078647.3  CG9907-RA, transcript variant A (para), mRNA 
0   0  2  10  35  NM_001043265.1  CG34139-RA (CG34139), mRNA 
0   0  2  9  22  NM_132479.2  CG1597-RA (CG1597), mRNA 
0   0  2  8  94  NM_001038766.1  CG32529-RD, transcript variant D (CG32529), mRNA 
0   0  2  8  86  NM_167688.1  CG32529-RC, transcript variant C (CG32529), mRNA 
0   0  2  8  35  NM_168412.1  CG32062-RD, transcript variant D (CG32062), mRNA 

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