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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
discarded
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
17083R-2 |
| Symbol |
CG17083 |
Full Name |
CG17083 |
| CG No |
CG17083 |
Old CG No |
CG17083 |
| Synonyms |
CG17083 |
| Accession No (Link to NCBI) |
NM_142882.1 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Ohhara Y, Nakamura A, Kato Y, Yamakawa-Kobayashi K.
Chaperonin TRiC/CCT supports mitotic exit and entry into endocycle in Drosophila.
PLoS Genet (2019) 15(4) e1008121
[
PubMed ID = 31034473
]
[
RRC reference
]
Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624
[
PubMed ID = 21666802
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgAGCTACCTGCTGAAGCACAAG |
| Primer Seq. 3' |
aatctagaggtaccCGATGATTCAGGATGCGGATA |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 AGCTACCTGC TGAAGCACAA GGAATTGGGC AAGCGACagC GNGAGTACAA GAAGATCCTG
0061 TCCAGCAAAC TCAAGACCCG CaANGAGACC ATCATCGAGG CCAGCTTTGA GACGGCCATC
0121 GATCAGCTGG AGGAGGAGAA AAACGATCTG CGTGCCCAGA TCTGGGTGGC CAGTGGTACG
0181 ACCTCACAAA AAACAGAACA ATCGCTGGCT GGAGCTGATT CGCTGCCATG TCGAGTGCCA
0241 GGAGAATCTG TCGCAGGACA TTAACTCTCT GAAGACATCG ATTTCCAACA TGGAGAAGGA
0301 GATAAGTCGC ATGTCCAAGC AAATATACAA CCTGAATCGT TTAACCATTC CGGATCAGCA
0361 ACATCAGGCC TATGTAATGC GAGCACGGAA AAGGATGACC ATTTTGGAGA ATTCCCTGGA
0421 GGTGGGAGTG CGTCAGGAGT GCGGTTTTAC GGCAGCCAAT GCGGATCTGC GAGAGCAGCT
0481 TATCCGCATC CTGAATCATC G |
| in silico PCR Fragment |
0001 AGCTACCTGC TGAAGCACAA GGAATTGGGC AAGCGACAGC GCGAGTACAA GAAGATCCTG
0061 TCCAGCAAAC TCAAGACCCG CAAGGAGACC ATCATCGAGG CCAGCTTTGA GACGGCCATC
0121 GATCAGCTGG AGGAGGAGAA AAACGATCTG CGTGCCCAGA TCTGGGTGGC CAGTGGTACG
0181 ACC-CACAAA AAACAGAACA ATCGCTGGCT GGAGCTGATT CGCTGCCATG TCGAGTGCCA
0241 GGAGAATCTG TCGCAGGACA TTAACTCTCT GAAGACATCG ATTTCCAACA TGGAGAAGGA
0301 GATAAGTCGC ATGTCCAAGC AAATATACAA CCTGAATCGT TTAACCATTC CGGATCAGCA
0361 ACATCAGGCC TATGTAATGC GAGCACGGAA AAGGATGACC ATTTTGGAGA ATTCCCTGGA
0421 GGTGGGAGTG CGTCAGGAGT GCGGTTTTAC GGCAGCCAAT GCGGATCTGC GAGAGCAGCT
0481 TATCCGCATC CTGAATCATC G |
| Assemble Data |
17083R-2.IR_full 1 AGCTACCTGCTGAAGCACAAGGAATTGGGCAAGCGACAGCGNGAGTACAAGAAGATCCTG 60
||||||||||||||||||||||||||||||||||||||||| ||||||||||||||||||
17083R-2.in silico 1 AGCTACCTGCTGAAGCACAAGGAATTGGGCAAGCGACAGCGCGAGTACAAGAAGATCCTG 60
17083R-2.IR_full 61 TCCAGCAAACTCAAGACCCGCAANGAGACCATCATCGAGGCCAGCTTTGAGACGGCCATC 120
||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||||
17083R-2.in silico 61 TCCAGCAAACTCAAGACCCGCAAGGAGACCATCATCGAGGCCAGCTTTGAGACGGCCATC 120
17083R-2.IR_full 121 GATCAGCTGGAGGAGGAGAAAAACGATCTGCGTGCCCAGATCTGGGTGGCCAGTGGTACG 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17083R-2.in silico 121 GATCAGCTGGAGGAGGAGAAAAACGATCTGCGTGCCCAGATCTGGGTGGCCAGTGGTACG 180
17083R-2.IR_full 181 ACCTCACAAAAAACAGAACAATCGCTGGCTGGAGCTGATTCGCTGCCATGTCGAGTGCCA 240
||| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17083R-2.in silico 181 ACC-CACAAAAAACAGAACAATCGCTGGCTGGAGCTGATTCGCTGCCATGTCGAGTGCCA 240
17083R-2.IR_full 241 GGAGAATCTGTCGCAGGACATTAACTCTCTGAAGACATCGATTTCCAACATGGAGAAGGA 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17083R-2.in silico 241 GGAGAATCTGTCGCAGGACATTAACTCTCTGAAGACATCGATTTCCAACATGGAGAAGGA 300
17083R-2.IR_full 301 GATAAGTCGCATGTCCAAGCAAATATACAACCTGAATCGTTTAACCATTCCGGATCAGCA 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17083R-2.in silico 301 GATAAGTCGCATGTCCAAGCAAATATACAACCTGAATCGTTTAACCATTCCGGATCAGCA 360
17083R-2.IR_full 361 ACATCAGGCCTATGTAATGCGAGCACGGAAAAGGATGACCATTTTGGAGAATTCCCTGGA 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17083R-2.in silico 361 ACATCAGGCCTATGTAATGCGAGCACGGAAAAGGATGACCATTTTGGAGAATTCCCTGGA 420
17083R-2.IR_full 421 GGTGGGAGTGCGTCAGGAGTGCGGTTTTACGGCAGCCAATGCGGATCTGCGAGAGCAGCT 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17083R-2.in silico 421 GGTGGGAGTGCGTCAGGAGTGCGGTTTTACGGCAGCCAATGCGGATCTGCGAGAGCAGCT 480
17083R-2.IR_full 481 TATCCGCATCCTGAATCATCG 501
|||||||||||||||||||||
17083R-2.in silico 481 TATCCGCATCCTGAATCATCG 501
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
1 |
NM_142882.1 |
CG17083-RA (CG17083), mRNA |
| 0.2 |
1 |
3 |
3 |
3 |
NM_140370.1 |
CG11274-RA (SRm160), mRNA |
| 0 |
0 |
3 |
2 |
5 |
NM_079902.2 |
CG6741-RB, transcript variant B (a), mRNA |
| 0 |
0 |
3 |
2 |
5 |
NM_166514.1 |
CG6741-RA, transcript variant A (a), mRNA |
| 0 |
0 |
3 |
2 |
5 |
NM_001014543.1 |
CG6741-RC, transcript variant C (a), mRNA |
| 0 |
0 |
2 |
5 |
4 |
NM_170324.1 |
CG31064-RB, transcript variant B (CG31064), mRNA |
| 0 |
0 |
2 |
3 |
8 |
NM_144448.2 |
CG1915-RC, transcript variant C (sls), mRNA |
| 0 |
0 |
2 |
3 |
7 |
NM_079937.2 |
CG1915-RA, transcript variant A (sls), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_139779.1 |
CG7386-RA (CG7386), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_139371.1 |
CG13917-RA (CG13917), mRNA |
| 0 |
0 |
2 |
2 |
7 |
NM_143585.2 |
CG11318-RA (CG11318), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_140367.2 |
CG11255-RA, transcript variant A (CG11255), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_168532.1 |
CG11255-RB, transcript variant B (CG11255), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_132794.2 |
CG18210-RA (CG18210), mRNA |
| 0 |
0 |
2 |
1 |
3 |
NM_080378.2 |
CG18210-RA (CG18210), mRNA,2-N-acetylglucosaminyltransferase I CG13431-RA (Mgat1), mRNA |
| 0 |
0 |
1 |
5 |
9 |
NM_132301.3 |
CG7065-RA (CG7065), mRNA |
| 0 |
0 |
1 |
5 |
8 |
NM_079176.1 |
CG12008-RA, transcript variant A (kst), mRNA |
| 0 |
0 |
1 |
5 |
8 |
NM_206266.1 |
CG12008-RB, transcript variant B (kst), mRNA |
| 0 |
0 |
1 |
5 |
8 |
NM_206265.1 |
CG12008-RC, transcript variant C (kst), mRNA |
| 0 |
0 |
1 |
4 |
12 |
NM_142959.2 |
CG6129-RB, transcript variant B (CG6129), mRNA |
| 0 |
0 |
1 |
4 |
11 |
NM_135223.2 |
CG11199-RA, transcript variant A (Liprin-alpha), mRNA |
| 0 |
0 |
1 |
4 |
11 |
NM_164707.1 |
CG11199-RB, transcript variant B (Liprin-alpha), mRNA |
| 0 |
0 |
1 |
4 |
8 |
NM_176553.1 |
CG6129-RC, transcript variant C (CG6129), mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_080139.3 |
CG8171-RA (dup), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_140918.1 |
CG14185-RA (CG14185), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_135304.2 |
CG7154-RA (CG7154), mRNA |
| 0 |
0 |
1 |
3 |
7 |
NM_205901.1 |
CG3304-RC, transcript variant C (CG3304), mRNA |
| 0 |
0 |
1 |
3 |
7 |
NM_134926.2 |
CG3304-RA, transcript variant A (CG3304), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_141841.1 |
CG10535-RA (CG10535), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_136859.2 |
CG8991-RA (Sobp), mRNA |
|
| Wing Disc |
GFP
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Puc
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Caspase
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DAPI
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Merge
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Figure
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JNK signal
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Caspase-3 signal
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