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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 1705R-2 
 Symbol Met  Full Name Methoprene-tolerant 
 CG No CG1705  Old CG No CG1705 
 Synonyms Met, met, Mett, CG1705, Rst(1)JH 
 Accession No (Link to NCBI) NM_078571.2 
 Inserted Chr. ll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees semi-lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Nitta Y, Yamazaki D, Sugie A, Hiroi M, Tabata T.
DISCO Interacting Protein 2 regulates axonal bifurcation and guidance of Drosophila mushroom body neurons.
Dev Biol (2017) 421(2) 233-244 [ PubMed ID = 27908785 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgCCACCCATGGCCTGAGACTTC 
 Primer Seq. 3' 
aatctagaggtaccGTGATCCTCGTCATCGTCCGC 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 CCACCCATGG CCTGAGACTT CAGTATGTCT TTGGCAAGTC CGCTTCCAGA CGTCGCAAGA 
0061 AACCCGGCCT CAAGGGAACG GGTATGTCTG CCTCACCTGT CGGAGATCTA CCCAATCCCA 
0121 GTCTGCATCT AACGGACACT CTAATGCAAC TGCTGGACTG CTGCTTCCTC ACCCTAACCT 
0181 GCAGTGGCCA AATCGTTTTG GTATCCACCA GCGTGGAGCA GCTATTGGGT CACTGTCAGT 
0241 CCGATTTGTA TGGCCAGAAT CTACTGCAGA TCACGCATCC CGATGATCAG GATCTGTTAA 
0301 GACAGCAGCT AATACCCAGG GATATAGAGA CCCTGTTCTA TCAGCATCAG CACCACCAGC 
0361 AGCAGGGGCA CAATCCCCAG CAGCACTCCA CTTCCACGTC GGCCTCAGCT TCGGGCAGTG 
0421 ATCTGGAGGA GGAGGAAATG GAGACGGAGG AACACCGTCT GGGTCGGCAG CAGGGAGAGG 
0481 CGGACGATGA CGAGGATCAC  
 in silico PCR Fragment 
0001 CCACCCATGG CCTGAGACTT CAGTATGTCT TTGGCAAGTC CGCTTCCAGA CGTCGCAAGA 
0061 AAACCGGCCT CAAGGGAACG GGTATGTCTG CCTCACCTGT CGGAGATCTA CCCAATCCCA 
0121 GTCTGCATCT AACGGACACT CTAATGCAAC TGCTGGACTG CTGCTTCCTC ACCCTAACCT 
0181 GCAGTGGCCA AATCGTTTTG GTATCCACCA GCGTGGAGCA GCTATTGGGT CACTGTCAGT 
0241 CCGATTTGTA TGGCCAGAAT CTACTGCAGA TCACGCATCC CGATGATCAG GATCTGTTAA 
0301 GACAGCAGCT AATACCCAGG GATATAGAGA CCCTGTTCTA TCAGCATCAG CACCACCAGC 
0361 AGCAGGGGCA CAATCCCCAG CAGCACTCCA CTTCCACGTC GGCCTCAACT TCGGGCAGTG 
0421 ATCTGGAGGA GGAGGAAATG GAGACGGAGG AACACCGTCT GGGTCGGCAG CAGGGAGAGG 
0481 CGGACGATGA CGAGGATCAC  
 Assemble Data 

1705R-2.IR_full       1   CCACCCATGGCCTGAGACTTCAGTATGTCTTTGGCAAGTCCGCTTCCAGACGTCGCAAGA 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1705R-2.in silico     1   CCACCCATGGCCTGAGACTTCAGTATGTCTTTGGCAAGTCCGCTTCCAGACGTCGCAAGA 60


1705R-2.IR_full       61  AACCCGGCCTCAAGGGAACGGGTATGTCTGCCTCACCTGTCGGAGATCTACCCAATCCCA 120
                          || |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1705R-2.in silico     61  AAACCGGCCTCAAGGGAACGGGTATGTCTGCCTCACCTGTCGGAGATCTACCCAATCCCA 120


1705R-2.IR_full       121 GTCTGCATCTAACGGACACTCTAATGCAACTGCTGGACTGCTGCTTCCTCACCCTAACCT 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1705R-2.in silico     121 GTCTGCATCTAACGGACACTCTAATGCAACTGCTGGACTGCTGCTTCCTCACCCTAACCT 180


1705R-2.IR_full       181 GCAGTGGCCAAATCGTTTTGGTATCCACCAGCGTGGAGCAGCTATTGGGTCACTGTCAGT 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1705R-2.in silico     181 GCAGTGGCCAAATCGTTTTGGTATCCACCAGCGTGGAGCAGCTATTGGGTCACTGTCAGT 240


1705R-2.IR_full       241 CCGATTTGTATGGCCAGAATCTACTGCAGATCACGCATCCCGATGATCAGGATCTGTTAA 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1705R-2.in silico     241 CCGATTTGTATGGCCAGAATCTACTGCAGATCACGCATCCCGATGATCAGGATCTGTTAA 300


1705R-2.IR_full       301 GACAGCAGCTAATACCCAGGGATATAGAGACCCTGTTCTATCAGCATCAGCACCACCAGC 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1705R-2.in silico     301 GACAGCAGCTAATACCCAGGGATATAGAGACCCTGTTCTATCAGCATCAGCACCACCAGC 360


1705R-2.IR_full       361 AGCAGGGGCACAATCCCCAGCAGCACTCCACTTCCACGTCGGCCTCAGCTTCGGGCAGTG 420
                          ||||||||||||||||||||||||||||||||||||||||||||||| ||||||||||||
1705R-2.in silico     361 AGCAGGGGCACAATCCCCAGCAGCACTCCACTTCCACGTCGGCCTCAACTTCGGGCAGTG 420


1705R-2.IR_full       421 ATCTGGAGGAGGAGGAAATGGAGACGGAGGAACACCGTCTGGGTCGGCAGCAGGGAGAGG 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1705R-2.in silico     421 ATCTGGAGGAGGAGGAAATGGAGACGGAGGAACACCGTCTGGGTCGGCAGCAGGGAGAGG 480


1705R-2.IR_full       481 CGGACGATGACGAGGATCAC 500
                          ||||||||||||||||||||
1705R-2.in silico     481 CGGACGATGACGAGGATCAC 500



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_078571.2  CG1705-RA (Rst(1)JH), mRNA 
0.41   2  2  4  83  NM_169386.1  CG17228-RA, transcript variant A (pros), mRNA 
0.41   2  2  4  73  NM_176459.1  CG17228-RD, transcript variant D (pros), mRNA 
0.41   2  2  4  73  NM_079593.3  CG17228-RC, transcript variant C (pros), mRNA 
0.2   1  9  17  16  NM_078605.1  CG6211-RA (gce), mRNA 
0.2   1  5  12  27  NM_079155.2  CG9102-RA (bab2), mRNA 
0.2   1  5  3  4  NM_140260.1  CG14129-RA (CG14129), mRNA 
0   0  16  25  60  NM_080120.2  CG14560-RA (msopa), mRNA 
0   0  9  3  20  NM_164653.1  CG31646-RA (CG31646), mRNA 
0   0  8  8  32  NM_168497.1  CG32105-RB (CG32105), mRNA 
0   0  7  8  18  NM_167487.1  CG32577-RA (disco-r), mRNA 
0   0  7  6  45  NM_080086.1  CG9930-RA (E5), mRNA 
0   0  6  18  84  NM_001014500.1  CG33558-RA (CG33558), mRNA 
0   0  6  7  45  NM_168007.1  CG11505-RA, transcript variant A (CG11505), mRNA 
0   0  6  7  45  NM_001038878.1  CG2956-RB, transcript variant B (twi), mRNA 
0   0  6  7  45  NM_139536.1  CG11505-RB, transcript variant B (CG11505), mRNA 
0   0  6  7  45  NM_079092.2  CG2956-RA, transcript variant A (twi), mRNA 
0   0  5  11  110  NM_057496.3  CG5058-RC, transcript variant C (grh), mRNA 
0   0  5  11  110  NM_057497.3  CG5058-RD, transcript variant D (grh), mRNA 
0   0  5  10  89  NM_079409.2  CG8127-RA, transcript variant A (Eip75B), mRNA 
0   0  5  7  38  NM_136397.1  CG15236-RA, transcript variant A (CG15236), mRNA 
0   0  5  7  38  NM_165493.1  CG15236-RB, transcript variant B (CG15236), mRNA 
0   0  5  4  41  NM_166252.1  CG5058-RG, transcript variant G (grh), mRNA 
0   0  5  4  9  NM_078604.3  CG32592-RA (hiw), mRNA 
0   0  5  4  6  NM_166920.1  CG4376-RB, transcript variant B (Actn), mRNA 
0   0  5  4  6  NM_058137.3  CG4376-RC, transcript variant C (Actn), mRNA 
0   0  5  4  6  NM_058136.3  CG4376-RA, transcript variant A (Actn), mRNA 
0   0  5  3  36  NM_176133.3  CG12052-RI, transcript variant I (lola), mRNA 
0   0  4  17  96  NM_001032244.1  CG32904-RA, transcript variant A (seq), mRNA 
0   0  4  12  47  NM_139420.1  CG12361-RA (CG12361), mRNA 

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