NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

17036R-1

Symbol

CG17036

Full Name

CG17036

CG No

CG17036

Old CG No

CG17036

Synonyms

CG17036

Accession No (Link to NCBI)

NM_135740.1

Inserted Chr.

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

viable

Map Viewer

Reference
[Please submit your publication]

Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgTCGCTGCTGCTGTGCACGTTT

Primer Seq. 3'

aatctagaggtaccATTAGCTGCGTGGCCAGCGAG

Predicted Fragment Size

IR fragment full Seq

0001 TCGCTGCTGC TGTGCACGTT TGGCTTTTTC CGGGAGTTTC GACCTTCGGA GCCGTACGTC 
0061 ACAGAGTACC TAGCCTCGGA TTATGGCAAC CTGACGACGG ATCAAATCTA TCAGGATGTG 
0121 TATCCCTTCG GCACGTACTC GGTGCTGGCC CAGCTGGTAA TTGTGTTCCT CATCACAGAT 
0181 CTGGTGAGGT ACAAACCGGT TATCGTGCTA TCGGCACTGG CTGGCATTAC CCTGTTCTCC 
0241 ATGCTTATCT GGACGGAAAC CGTGGGAATG CTGCAGGTGG CCCAAGTGTT TTTTGGCACC 
0301 TTTACGGCCG CTGAGGTGGC CTACTACACG TACATGTACG CCAAGGTCAA CAAGGAGCAG 
0361 TATCAGGTAG TCACGGGCCA CACGCGAGCT GCCATCTTGA GCGGCAAGTT CCTCGGCGGC 
0421 CTGTTTGCCC AGATTTTGGT GTCCACCGAT AGCATGGATT ACCGGCAGCT GCACTACCTC 
0481 NTCGCTGGCC ACGCAGCTAA T

in silico PCR Fragment

0001 TCGCTGCTGC TGTGCACGTT TGGCTTTTTC CGGGAGTTTC GACCTTCGGA GCCGTACGTC 
0061 ACAGAGTACC TAGCCTCGGA TTATGGCAAC CTGACGACGG ATCAAATCTA TCAGGATGTG 
0121 TATCCCTTCG GCACGTACTC GGTGCTGGCC CAGCTGGTAA TTGTGTTCCT CATCACAGAT 
0181 CTGGTGAGGT ACAAACCGGT TATCGTGCTA TCGGCACTGG CTGGCATTAC CCTGTTCTCC 
0241 ATGCTTATCT GGACGGAAAC CGTGGGAATG CTGCAGGTGG CCCAAGTGTT TTTTGGCACC 
0301 TTTACGGCCG CTGAGGTGGC CTACTACACG TACATGTACG CCAAGGTCAA CAAGGAGCAG 
0361 TATCAGGTAG TCACGGGCCA CACGCGAGCT GCCATCTTGA GCGGCAAGTT CCTCGGCGGC 
0421 CTGTTTGCCC AGATTTTGGT GTCCACCGAT AGCATGGATT ACCGGCAGCT GCACTACCTC 
0481 -TCGCTGGCC ACGCAGCTAA T

Assemble Data


17036R-1.IR_full       1   TCGCTGCTGCTGTGCACGTTTGGCTTTTTCCGGGAGTTTCGACCTTCGGAGCCGTACGTC 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17036R-1.in silico     1   TCGCTGCTGCTGTGCACGTTTGGCTTTTTCCGGGAGTTTCGACCTTCGGAGCCGTACGTC 60


17036R-1.IR_full       61  ACAGAGTACCTAGCCTCGGATTATGGCAACCTGACGACGGATCAAATCTATCAGGATGTG 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17036R-1.in silico     61  ACAGAGTACCTAGCCTCGGATTATGGCAACCTGACGACGGATCAAATCTATCAGGATGTG 120


17036R-1.IR_full       121 TATCCCTTCGGCACGTACTCGGTGCTGGCCCAGCTGGTAATTGTGTTCCTCATCACAGAT 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17036R-1.in silico     121 TATCCCTTCGGCACGTACTCGGTGCTGGCCCAGCTGGTAATTGTGTTCCTCATCACAGAT 180


17036R-1.IR_full       181 CTGGTGAGGTACAAACCGGTTATCGTGCTATCGGCACTGGCTGGCATTACCCTGTTCTCC 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17036R-1.in silico     181 CTGGTGAGGTACAAACCGGTTATCGTGCTATCGGCACTGGCTGGCATTACCCTGTTCTCC 240


17036R-1.IR_full       241 ATGCTTATCTGGACGGAAACCGTGGGAATGCTGCAGGTGGCCCAAGTGTTTTTTGGCACC 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17036R-1.in silico     241 ATGCTTATCTGGACGGAAACCGTGGGAATGCTGCAGGTGGCCCAAGTGTTTTTTGGCACC 300


17036R-1.IR_full       301 TTTACGGCCGCTGAGGTGGCCTACTACACGTACATGTACGCCAAGGTCAACAAGGAGCAG 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17036R-1.in silico     301 TTTACGGCCGCTGAGGTGGCCTACTACACGTACATGTACGCCAAGGTCAACAAGGAGCAG 360


17036R-1.IR_full       361 TATCAGGTAGTCACGGGCCACACGCGAGCTGCCATCTTGAGCGGCAAGTTCCTCGGCGGC 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17036R-1.in silico     361 TATCAGGTAGTCACGGGCCACACGCGAGCTGCCATCTTGAGCGGCAAGTTCCTCGGCGGC 420


17036R-1.IR_full       421 CTGTTTGCCCAGATTTTGGTGTCCACCGATAGCATGGATTACCGGCAGCTGCACTACCTC 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17036R-1.in silico     421 CTGTTTGCCCAGATTTTGGTGTCCACCGATAGCATGGATTACCGGCAGCTGCACTACCTC 480


17036R-1.IR_full        481 TCGCTGGCCACGCAGCTAAT 500
                            ||||||||||||||||||||
17036R-1.in silico      481 TCGCTGGCCACGCAGCTAAT 500

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_135740.1	CG17036-RA (CG17036), mRNA
0.2	1	2	3	4	NM_139366.2	CG12099-RB, transcript variant B (CG12099), mRNA
0.2	1	2	3	4	NM_167895.1	CG12099-RA, transcript variant A (CG12099), mRNA
0	0	8	19	21	NM_141772.1	CG6574-RA (CG6574), mRNA
0	0	6	15	23	NM_141771.1	CG14694-RA (CG14694), mRNA
0	0	2	3	3	NM_138209.2	CG3279-RA (CG3279), mRNA
0	0	1	5	5	NM_136365.1	CG11212-RA (Ptr), mRNA
0	0	1	5	4	NM_166619.1	CG5411-RC, transcript variant C (Pde8), mRNA
0	0	1	5	4	NM_166617.1	CG5411-RD, transcript variant D (Pde8), mRNA
0	0	1	5	4	NM_166618.1	CG5411-RB, transcript variant B (Pde8), mRNA
0	0	1	5	4	NM_137970.3	CG5411-RE, transcript variant E (Pde8), mRNA
0	0	1	5	4	NM_166616.1	CG5411-RA, transcript variant A (Pde8), mRNA
0	0	1	4	4	NM_142913.1	CG10254-RA, transcript variant A (CG10254), mRNA
0	0	1	4	4	NM_170081.1	CG10254-RB, transcript variant B (CG10254), mRNA
0	0	1	3	3	NM_132370.1	CG2989-RA (CG2989), mRNA
0	0	1	2	13	NM_142682.1	CG3421-RA (RhoGAP93B), mRNA
0	0	1	2	3	NM_078776.4	CG31629-RA, transcript variant A (Pvf3), mRNA
0	0	0	5	13	NM_136041.1	CG10231-RA (Pde11), mRNA
0	0	0	5	6	NM_167965.1	CG32296-RA (Mrtf), mRNA
0	0	0	5	3	NM_133129.2	CG7635-RA (Mec2), mRNA
0	0	0	4	14	NM_141683.2	CG8417-RA (CG8417), mRNA
0	0	0	4	11	NM_132709.1	CG12480-RA (CG12480), mRNA
0	0	0	4	8	NM_170158.1	CG5794-RB, transcript variant B (CG5794), mRNA
0	0	0	4	8	NM_143018.2	CG5794-RD, transcript variant D (CG5794), mRNA
0	0	0	4	8	NM_206564.1	CG5794-RE, transcript variant E (CG5794), mRNA
0	0	0	4	6	NM_167080.1	CG32742-RA (l(1)G0148), mRNA
0	0	0	4	6	NM_079409.2	CG8127-RA, transcript variant A (Eip75B), mRNA
0	0	0	4	5	NM_168756.1	CG8127-RC, transcript variant C (Eip75B), mRNA
0	0	0	4	5	NM_168755.1	CG8127-RB, transcript variant B (Eip75B), mRNA
0	0	0	4	5	NM_168757.1	CG8127-RD, transcript variant D (Eip75B), mRNA

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