NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 16993R-2

Symbol in Full Name inturned

CG No CG16993 Old CG No CG16993

Synonyms B, CG16993, in

Accession No (Link to NCBI) NM_176370.1

Inserted Chr. ll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees viable

Map Viewer

Reference
[Please submit your publication] Mummery-Widmer JL, Yamazaki M, Stoeger T, Novatchkova M, Bhalerao S, Chen D, Dietzl G, Dickson BJ, Knoblich JA.
Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi.
Nature (2009) 458(7241) 987-92 [ PubMed ID = 19363474 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCGTCGGAGGCCTTCTCCAGTT

Primer Seq. 3'

aatctagaggtaccGTGCTGCAGCGCACTCACATC

Predicted Fragment Size

IR fragment full Seq

0001 CGTCGGAGGC CTTCTCCAGT TCGAACTCCT CGCTGAGCAA CTCGTACAGC GATGGCAGCG 
0061 ACCTGGCCAA CTGGGAGGAG TACGTGTCCA CAGACGGCAG TCTCTTCTAC ATGGAGTATG 
0121 TGCGATGTGA GAAGACTGGC GTCTCGGCAG AGCGGCGGGT GGAGTTCATG CGTCGATCGC 
0181 TGCGCCTGGG AAAGAAGTCG TCGCGTCGCC AGCCGCACAG CAATAATCAG AATCAGAGTC 
0241 AGAACCAAAA CCAGTCCCAC GGCCCGAGCA GGTTGGATAG CCAGACGGAG CCGCAAGAGT 
0301 TCCGCTTCTC ACAGTTCAAG ACGGCTGCCC CCGATCCCAA GAAACTGGAT CTGGTGATCA 
0361 CAGCCGCCGA TCGCTTCCGT TTCGGACGAC GATCCACGGC GGTGGAGTCC ATACTGGGCT 
0421 TCCGGGTCCT GCCCTTTCCG GACCAACCAG AATGCCTGAT GGTCGATGGG TTCGTTCACG 
0481 ATGTGAGTgN GCTGCAGCAC

in silico PCR Fragment

0001 CGTCGGAGGC CTTCTCCAGT TCGAACTCCT CGCTGAGCAA CTCGTACAGC GATGGCAGCG 
0061 ACCTGGCCAA CTGGGAGGAG TACGTGTCCA CAGACGGCAG TCTCTTCTAC ATGGAGTATG 
0121 TGCGATGTGA GAAGACTGGC GTCTCGGCAG AGCGGCGGGT GGAGTTCATG CGTCGATCGC 
0181 TGCGCCTGGG AAAGAAGTCG TCGCGTCGCC AGCCGCACAG CAATAATCAG AATCAGAGTC 
0241 AGAACCAAAA CCAGTCCCAC GGCCCGAGCA GGTTGGATAG CCAGACGGAG CCGCAAGAGT 
0301 TCCGCTTCTC ACAGTTCAAG ACGGCTGCCC CCGATCCCAA GAAACTGGAT CTGGTGATCA 
0361 CAGCCGCTGA TCGCTTCCGT TTCGGACGAC GATCCACGGC GGTGGAGTCC ATACTGGGCT 
0421 TCCGGGTCCT GCCCTTTCCG GACCAACCAG AATGCCTGAT GGTCGATGGG TTCGTTCACG 
0481 ATGTGAGTGC GCTGCAGCAC

Assemble Data


16993R-2.IR_full       1   CGTCGGAGGCCTTCTCCAGTTCGAACTCCTCGCTGAGCAACTCGTACAGCGATGGCAGCG 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
16993R-2.in silico     1   CGTCGGAGGCCTTCTCCAGTTCGAACTCCTCGCTGAGCAACTCGTACAGCGATGGCAGCG 60


16993R-2.IR_full       61  ACCTGGCCAACTGGGAGGAGTACGTGTCCACAGACGGCAGTCTCTTCTACATGGAGTATG 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
16993R-2.in silico     61  ACCTGGCCAACTGGGAGGAGTACGTGTCCACAGACGGCAGTCTCTTCTACATGGAGTATG 120


16993R-2.IR_full       121 TGCGATGTGAGAAGACTGGCGTCTCGGCAGAGCGGCGGGTGGAGTTCATGCGTCGATCGC 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
16993R-2.in silico     121 TGCGATGTGAGAAGACTGGCGTCTCGGCAGAGCGGCGGGTGGAGTTCATGCGTCGATCGC 180


16993R-2.IR_full       181 TGCGCCTGGGAAAGAAGTCGTCGCGTCGCCAGCCGCACAGCAATAATCAGAATCAGAGTC 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
16993R-2.in silico     181 TGCGCCTGGGAAAGAAGTCGTCGCGTCGCCAGCCGCACAGCAATAATCAGAATCAGAGTC 240


16993R-2.IR_full       241 AGAACCAAAACCAGTCCCACGGCCCGAGCAGGTTGGATAGCCAGACGGAGCCGCAAGAGT 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
16993R-2.in silico     241 AGAACCAAAACCAGTCCCACGGCCCGAGCAGGTTGGATAGCCAGACGGAGCCGCAAGAGT 300


16993R-2.IR_full       301 TCCGCTTCTCACAGTTCAAGACGGCTGCCCCCGATCCCAAGAAACTGGATCTGGTGATCA 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
16993R-2.in silico     301 TCCGCTTCTCACAGTTCAAGACGGCTGCCCCCGATCCCAAGAAACTGGATCTGGTGATCA 360


16993R-2.IR_full       361 CAGCCGCCGATCGCTTCCGTTTCGGACGACGATCCACGGCGGTGGAGTCCATACTGGGCT 420
                           ||||||| ||||||||||||||||||||||||||||||||||||||||||||||||||||
16993R-2.in silico     361 CAGCCGCTGATCGCTTCCGTTTCGGACGACGATCCACGGCGGTGGAGTCCATACTGGGCT 420


16993R-2.IR_full       421 TCCGGGTCCTGCCCTTTCCGGACCAACCAGAATGCCTGATGGTCGATGGGTTCGTTCACG 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
16993R-2.in silico     421 TCCGGGTCCTGCCCTTTCCGGACCAACCAGAATGCCTGATGGTCGATGGGTTCGTTCACG 480


16993R-2.IR_full       481 ATGTGAGTGNGCTGCAGCAC 500
                           ||||||||| ||||||||||
16993R-2.in silico     481 ATGTGAGTGCGCTGCAGCAC 500

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_176370.1	CG16993-RA (in), mRNA
0.62	3	2	7	5	NM_138014.1	CG13566-RA (CG13566), mRNA
0	0	5	10	23	NM_078811.2	CG5387-RA (Cdk5alpha), mRNA
0	0	3	3	4	NM_176510.1	CG31247-RD, transcript variant D (tinc), mRNA
0	0	3	3	4	NM_169780.2	CG31247-RB, transcript variant B (tinc), mRNA
0	0	3	3	4	NM_176511.1	CG31247-RC, transcript variant C (tinc), mRNA
0	0	3	3	4	NM_176509.1	CG31247-RA, transcript variant A (tinc), mRNA
0	0	2	13	20	NM_078499.3	CG4690-RA (Tsp5D), mRNA
0	0	2	12	26	NM_143361.3	CG1866-RB, transcript variant B (Moca-cyp), mRNA
0	0	2	12	26	NM_170367.1	CG1866-RA, transcript variant A (Moca-cyp), mRNA
0	0	2	7	14	NM_176424.1	CG9821-RA, transcript variant A (CG9821), mRNA
0	0	2	7	14	NM_176425.1	CG9821-RB, transcript variant B (CG9821), mRNA
0	0	2	5	28	NM_132578.1	CG11146-RA (CG11146), mRNA
0	0	2	5	11	NM_143111.2	CG11891-RA, transcript variant A (CG11891), mRNA
0	0	2	5	11	NM_143110.2	CG11889-RA, transcript variant A (CG11889), mRNA
0	0	2	5	2	NM_132781.1	CG5662-RA (CG5662), mRNA
0	0	2	4	7	NM_205987.1	CG32972-RA, transcript variant A (CG32972), mRNA
0	0	2	3	5	NM_001032041.1	CG11891-RB, transcript variant B (CG11891), mRNA
0	0	2	3	5	NM_001032040.1	CG11891-RC, transcript variant C (CG11891), mRNA
0	0	2	3	4	NM_058033.3	CG7581-RA (Bub3), mRNA
0	0	2	2	6	NM_168670.1	CG32158-RB, transcript variant B (CG32158), mRNA
0	0	2	2	5	NM_168671.2	CG32158-RA, transcript variant A (CG32158), mRNA
0	0	2	2	5	NM_206386.1	CG32158-RF, transcript variant F (CG32158), mRNA
0	0	2	2	5	NM_140079.1	CG3280-RA (CG3280), mRNA
0	0	2	2	4	NM_134737.2	CG5118-RA (CG5118), mRNA
0	0	2	2	3	NM_136114.1	CG17350-RA (CG17350), mRNA
0	0	2	2	2	NM_141554.2	CG8032-RA (CG8032), mRNA
0	0	1	6	2	NM_167620.2	CG32547-RA (CG32547), mRNA
0	0	1	5	5	NM_141750.1	CG14684-RA (CG14684), mRNA
0	0	1	5	2	NM_175980.1	CG7102-RA (CG7102), mRNA

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