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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 16953R-1 
 Symbol CG16953  Full Name CG16953 
 CG No CG16953  Old CG No CG16953 
 Synonyms CG16953 
 Accession No (Link to NCBI) NM_142640.2 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624 [ PubMed ID = 21666802 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgCTCGCTCTTCTGCAGACAGTT 
 Primer Seq. 3' 
aatctagaggtaccCCGAGATATGTGCAGTTCTGA 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 CTCGCTCTTC TGCAGACAGT TACCTGCTAC ACCGTCATCA TCACTGCTCC CGAACAGGCC 
0061 CTCATCGGAT CCACCATCAA TGTGTCCGCC CAGCTCTTCG ATGGCGACAA ACCGGCTGAC 
0121 GGCGGCAGGC CCTACAGGTT CACATGGTCC GACAGTGCTG GCCATCGAAA GGTGATTGAA 
0181 ACGAACAGCG TTAGCAGCAA TTGGTCTGTG GTCTTCACGC AAGAACAACC GGCGACGCAT 
0241 GTCATCAAGG TGGAAGTGGA GAAGTTCGTC ACATTTTTTT GGGTGACGGA GGCTAAGGCC 
0301 TCGAAGGGGA ATCAAGCTGG ACAGCTTGCT CGGCGGCAGC CTGGAAGCTG AATTCAGAAC 
0361 AATGTGATGC GACCCCATGA GTTTGTGTCC ACCCAGGAGG CGGTGAATCA CAGCGTATCA 
0421 CTTTCCGATG CGGACCTACT TTTTCTGCAG TCGAAGGGTT ATCGCATTCT GACGTATTGG 
0481 TTTCAGAACT GCACATATCT CGG 
 in silico PCR Fragment 
0001 CTCGCTCTTC TGCAGACAGT TACCTGCTAC ACCGTCATCA TCACTGCTCC CGAACAGGCC 
0061 CTCATCGGAT CCACCATCAA TGTGTCCGCC CAGCTCTTCG ATGGCGACAA ACCGGCTGAC 
0121 GGAGGCAGGC CCTACAGGTT CACATGGTCC GACAGTGCTG GCCATCGAAA GGTGATTGAA 
0181 ACGAACAGCG TTAGCAGCAA TTGGTCTGTG GTCTTCACGC AAGAACAACC GGCGACGCAT 
0241 GTCATCAAGG TGGAAGTGGA GAAGTTCGTC ACATTTTTTT GGGTGACGGA GGCTAAGGCC 
0301 TCG-AGGGGA ATCAAGCTGG ACAGCTTGCT CGGCGGCAGC CTGG-AGCTG -ATTCAGAAC 
0361 AATGTGATGC GACCCCATGA GTTTGTGTCC ACCCAGGAGG CGGTGAATCA CAGCGTATCA 
0421 CTTTCCGATG CGGACCTACT TTTTCTGCAG TCGAAGGGTT ATCGCATTCT GACGTATTGG 
0481 TTTCAGAACT GCACATATCT CGG 
 Assemble Data 

16953R-1.IR_full       1   CTCGCTCTTCTGCAGACAGTTACCTGCTACACCGTCATCATCACTGCTCCCGAACAGGCC 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
16953R-1.in silico     1   CTCGCTCTTCTGCAGACAGTTACCTGCTACACCGTCATCATCACTGCTCCCGAACAGGCC 60


16953R-1.IR_full       61  CTCATCGGATCCACCATCAATGTGTCCGCCCAGCTCTTCGATGGCGACAAACCGGCTGAC 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
16953R-1.in silico     61  CTCATCGGATCCACCATCAATGTGTCCGCCCAGCTCTTCGATGGCGACAAACCGGCTGAC 120


16953R-1.IR_full       121 GGCGGCAGGCCCTACAGGTTCACATGGTCCGACAGTGCTGGCCATCGAAAGGTGATTGAA 180
                           || |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
16953R-1.in silico     121 GGAGGCAGGCCCTACAGGTTCACATGGTCCGACAGTGCTGGCCATCGAAAGGTGATTGAA 180


16953R-1.IR_full       181 ACGAACAGCGTTAGCAGCAATTGGTCTGTGGTCTTCACGCAAGAACAACCGGCGACGCAT 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
16953R-1.in silico     181 ACGAACAGCGTTAGCAGCAATTGGTCTGTGGTCTTCACGCAAGAACAACCGGCGACGCAT 240


16953R-1.IR_full       241 GTCATCAAGGTGGAAGTGGAGAAGTTCGTCACATTTTTTTGGGTGACGGAGGCTAAGGCC 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
16953R-1.in silico     241 GTCATCAAGGTGGAAGTGGAGAAGTTCGTCACATTTTTTTGGGTGACGGAGGCTAAGGCC 300


16953R-1.IR_full       301 TCGAAGGGGAATCAAGCTGGACAGCTTGCTCGGCGGCAGCCTGGAAGCTGAATTCAGAAC 360
                           ||| |||||||||||||||||||||||||||||||||||||||| ||||| |||||||||
16953R-1.in silico     301 TCG-AGGGGAATCAAGCTGGACAGCTTGCTCGGCGGCAGCCTGG-AGCTG-ATTCAGAAC 360


16953R-1.IR_full       361 AATGTGATGCGACCCCATGAGTTTGTGTCCACCCAGGAGGCGGTGAATCACAGCGTATCA 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
16953R-1.in silico     361 AATGTGATGCGACCCCATGAGTTTGTGTCCACCCAGGAGGCGGTGAATCACAGCGTATCA 420


16953R-1.IR_full       421 CTTTCCGATGCGGACCTACTTTTTCTGCAGTCGAAGGGTTATCGCATTCTGACGTATTGG 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
16953R-1.in silico     421 CTTTCCGATGCGGACCTACTTTTTCTGCAGTCGAAGGGTTATCGCATTCTGACGTATTGG 480


16953R-1.IR_full       481 TTTCAGAACTGCACATATCTCGG 503
                           |||||||||||||||||||||||
16953R-1.in silico     481 TTTCAGAACTGCACATATCTCGG 503



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_142640.2  CG16953-RA (CG16953), mRNA 
0   0  4  2  4  NM_164520.1  CG17224-RA, transcript variant A (CG17224), mRNA 
0   0  4  2  4  NM_134891.2  CG17224-RB, transcript variant B (CG17224), mRNA 
0   0  3  2  7  NM_001042810.1  CG10952-RB, transcript variant B (eag), mRNA 
0   0  2  6  4  NM_165314.1  CG10952-RB, transcript variant B (eag), mRNA,6-bisphosphatase CG31692-RB, transcript variant B (fbp), mRNA 
0   0  2  6  4  NM_136157.2  CG10952-RB, transcript variant B (eag), mRNA,6-bisphosphatase CG31692-RB, transcript variant B (fbp), mRNA,6-bisphosphatase CG31692-RA, transcript variant A (fbp), mRNA 
0   0  2  3  4  NM_079551.2  CG7494-RA (mRpL1), mRNA 
0   0  2  3  3  NM_137020.1  CG4734-RA (CG4734), mRNA 
0   0  2  2  4  NM_079761.2  CG13626-RA (Syx18), mRNA 
0   0  2  2  3  NM_140347.1  CG11008-RA (CG11008), mRNA 
0   0  2  2  2  NM_134517.2  CG15618-RA (CG15618), mRNA 
0   0  1  5  2  NM_140582.2  CG5215-RA, transcript variant A (Zn72D), mRNA 
0   0  1  5  2  NM_176332.1  CG5215-RB, transcript variant B (Zn72D), mRNA 
0   0  1  4  4  NM_167408.1  CG32600-RA (dpr8), mRNA 
0   0  1  2  5  NM_135840.2  CG7968-RA (CG7968), mRNA 
0   0  1  2  3  NM_164539.1  CG31956-RA (pgant4), mRNA 
0   0  1  2  2  NM_168383.1  CG6718-RB, transcript variant B (CG6718), mRNA 
0   0  1  2  2  NM_168385.1  CG6718-RD, transcript variant D (CG6718), mRNA 
0   0  1  2  2  NM_140109.1  CG6718-RA, transcript variant A (CG6718), mRNA 
0   0  1  2  2  NM_168384.1  CG6718-RC, transcript variant C (CG6718), mRNA 
0   0  1  2  2  NM_143570.3  CG31012-RB, transcript variant B (CG31012), mRNA 
0   0  1  2  2  NM_170527.2  CG31012-RA, transcript variant A (CG31012), mRNA 
0   0  1  2  2  NM_137163.3  CG10255-RA (Lap1), mRNA 
0   0  1  2  2  NM_170528.2  CG31012-RD, transcript variant D (CG31012), mRNA 
0   0  1  2  2  NM_170526.2  CG31012-RC, transcript variant C (CG31012), mRNA 
0   0  1  2  2  NM_206283.1  CG8398-RD, transcript variant D (CG8398), mRNA 
0   0  1  2  2  NM_139794.1  CG8398-RA, transcript variant A (CG8398), mRNA 
0   0  1  2  2  NM_168173.1  CG8398-RB, transcript variant B (CG8398), mRNA 
0   0  1  2  2  NM_168174.1  CG8398-RC, transcript variant C (CG8398), mRNA 
0   0  0  4  14  NM_134544.1  CG11734-RB (HERC2), mRNA 

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