NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 16894R-1

Symbol CG16894 Full Name CG16894

CG No CG16894 Old CG No CG16894

Synonyms CG16894

Accession No (Link to NCBI) NM_137611.1

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees viable

Map Viewer

Reference
[Please submit your publication] Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGGAACCCAAGTATTGCCCTCA

Primer Seq. 3'

aatctagaggtaccTGCTTGCTCCTGGACTCGTTT

Predicted Fragment Size

IR fragment full Seq

0001 GNAACCCAAG TATTGCCCTC ATCAAACAGG GCTACCATAT CCTCGCCGAG TACAATCTGG 
0061 TGAAGGACGA GCTGAAGAAC ATCTATGCCA TACCAAGTTA CGCTTGTGGA TTGCACTGGT 
0121 TCGGCGTCAT CTTTGTGCAC AGTGGAATCT ATGCGGGCAG CGTGNTTTCG CTTCTCCATT 
0181 CTGCTGCCGG ATAATTTTCC GGCGGACATC AGCCTGCCCA CGGTGGTCTT CTCGACGGAG 
0241 GTCCTACATC CGCACATCTG TCCGCAGAAT AAGACCCTCG ATCTGGCGCA TTTCCTGAAC 
0301 GAGTGGCGCA AGGATGAGCA CCACATCTGG CACGTGCTTC GCTATATCCA GGCTATTTTC 
0361 GCGGATCCAG AGGGCAGTAT TTGCACCGGA CAATCCTCTT CGGGGGATCT CGTGATCATG 
0421 GATGAGGTTA GAAACATGAA TGCCTTGAAT ATGTTGGCCA AAAGCCGGCC TGAATACATC 
0481 AAACGAGTCC AGGAGCAAGC A

in silico PCR Fragment

0001 GGAACCCAAG TATTGCCCTC ATCAAACAGG GCTACCATAT TCTCGCCGAA TACAATCTGG 
0061 TGAAGGAAGA GCTAAAGAAC ATCTATGCCA TACCCAGTTA CGCTTGTGGA TTGCACTGGT 
0121 TCGGCGTCAT CTTTGTGCAC AGTGGAATCT ATGCGGGCAG CGTG-TTTCG CTTCTCCATT 
0181 CTGCTGCCGG AAAATTTTCC GGCGGACATC AGCCTGCCCA CGGTGGTCTT CTCGACGGAG 
0241 GTCCTACATC CGCACATCTG TCCGCAGAAT AAGACCCTCG ATCTGGCGCA TTTCCTGAAC 
0301 GAGTGGCGCA AGGATGAGCA CCACATCTGG CACGTGCTTC GCTATATCCA GGCTATTTTC 
0361 GCGGATCCAG AGGGCAGTAT TTGCACCGGA CAATCCTCTT CGGGGGATCT CGTGATCATG 
0421 GATGAGGTTA GAAACATGAA TGCCTTGAAT ATGTTGGCCA AAAGCCGGCC TGAATACATC 
0481 AAACGAGTCC AGGAGCAAGC A

Assemble Data


16894R-1.IR_full       1   GNAACCCAAGTATTGCCCTCATCAAACAGGGCTACCATATCCTCGCCGAGTACAATCTGG 60
                           | |||||||||||||||||||||||||||||||||||||| |||||||| ||||||||||
16894R-1.in silico     1   GGAACCCAAGTATTGCCCTCATCAAACAGGGCTACCATATTCTCGCCGAATACAATCTGG 60


16894R-1.IR_full       61  TGAAGGACGAGCTGAAGAACATCTATGCCATACCAAGTTACGCTTGTGGATTGCACTGGT 120
                           ||||||| ||||| |||||||||||||||||||| |||||||||||||||||||||||||
16894R-1.in silico     61  TGAAGGAAGAGCTAAAGAACATCTATGCCATACCCAGTTACGCTTGTGGATTGCACTGGT 120


16894R-1.IR_full       121 TCGGCGTCATCTTTGTGCACAGTGGAATCTATGCGGGCAGCGTGNTTTCGCTTCTCCATT 180
                           |||||||||||||||||||||||||||||||||||||||||||| |||||||||||||||
16894R-1.in silico     121 TCGGCGTCATCTTTGTGCACAGTGGAATCTATGCGGGCAGCGTG-TTTCGCTTCTCCATT 180


16894R-1.IR_full       181 CTGCTGCCGGATAATTTTCCGGCGGACATCAGCCTGCCCACGGTGGTCTTCTCGACGGAG 240
                           ||||||||||| ||||||||||||||||||||||||||||||||||||||||||||||||
16894R-1.in silico     181 CTGCTGCCGGAAAATTTTCCGGCGGACATCAGCCTGCCCACGGTGGTCTTCTCGACGGAG 240


16894R-1.IR_full       241 GTCCTACATCCGCACATCTGTCCGCAGAATAAGACCCTCGATCTGGCGCATTTCCTGAAC 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
16894R-1.in silico     241 GTCCTACATCCGCACATCTGTCCGCAGAATAAGACCCTCGATCTGGCGCATTTCCTGAAC 300


16894R-1.IR_full       301 GAGTGGCGCAAGGATGAGCACCACATCTGGCACGTGCTTCGCTATATCCAGGCTATTTTC 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
16894R-1.in silico     301 GAGTGGCGCAAGGATGAGCACCACATCTGGCACGTGCTTCGCTATATCCAGGCTATTTTC 360


16894R-1.IR_full       361 GCGGATCCAGAGGGCAGTATTTGCACCGGACAATCCTCTTCGGGGGATCTCGTGATCATG 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
16894R-1.in silico     361 GCGGATCCAGAGGGCAGTATTTGCACCGGACAATCCTCTTCGGGGGATCTCGTGATCATG 420


16894R-1.IR_full       421 GATGAGGTTAGAAACATGAATGCCTTGAATATGTTGGCCAAAAGCCGGCCTGAATACATC 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
16894R-1.in silico     421 GATGAGGTTAGAAACATGAATGCCTTGAATATGTTGGCCAAAAGCCGGCCTGAATACATC 480


16894R-1.IR_full       481 AAACGAGTCCAGGAGCAAGCA 501
                           |||||||||||||||||||||
16894R-1.in silico     481 AAACGAGTCCAGGAGCAAGCA 501

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	4	2	2	NM_137611.1	CG16894-RA (CG16894), mRNA
0	0	2	2	3	NM_144374.1	CG16834-RA (lectin-33A), mRNA
0	0	1	6	2	NM_140649.2	CG17286-RA (CG17286), mRNA
0	0	1	5	4	NM_142092.2	CG14840-RA (CG14840), mRNA
0	0	1	4	2	NM_167525.1	CG9802-RB, transcript variant B (Cap), mRNA
0	0	1	4	2	NM_078650.2	CG9802-RA, transcript variant A (Cap), mRNA
0	0	1	3	7	NM_130604.2	CG17766-RA (CG17766), mRNA
0	0	1	2	4	NM_168992.2	CG10520-RA (tub), mRNA
0	0	1	2	3	NM_141285.1	CG14670-RA (CG14670), mRNA
0	0	1	2	3	NM_079677.2	CG6027-RA (cdi), mRNA
0	0	1	2	3	NM_169595.1	CG3508-RB, transcript variant B (CG3508), mRNA
0	0	1	2	3	NM_142124.2	CG3508-RA, transcript variant A (CG3508), mRNA
0	0	1	2	2	NM_206408.1	CG33285-RA (CG33285), mRNA
0	0	0	7	6	NM_141812.2	CG6723-RA (CG6723), mRNA
0	0	0	6	3	NM_165966.1	CG3991-RD, transcript variant D (TppII), mRNA
0	0	0	6	3	NM_057899.2	CG3991-RA, transcript variant A (TppII), mRNA
0	0	0	5	7	NM_141383.1	CG15189-RA (Osi19), mRNA
0	0	0	4	9	NM_165948.1	CG8766-RC, transcript variant C (tafazzin), mRNA
0	0	0	4	6	NM_136547.1	CG14748-RA (CG14748), mRNA
0	0	0	4	6	NM_058084.3	CG8766-RA, transcript variant A (tafazzin), mRNA
0	0	0	4	6	NM_165947.1	CG8766-RB, transcript variant B (tafazzin), mRNA
0	0	0	4	4	NM_140250.1	CG5964-RA (CG5964), mRNA
0	0	0	4	3	NM_143621.1	CG1792-RA (CG1792), mRNA
0	0	0	4	2	NM_169566.1	CG9924-RB, transcript variant B (CG9924), mRNA
0	0	0	4	2	NM_143380.3	CG1520-RA, transcript variant A (WASp), mRNA
0	0	0	4	2	NM_170378.2	CG1520-RB, transcript variant B (WASp), mRNA
0	0	0	4	2	NM_176578.1	CG1520-RC, transcript variant C (WASp), mRNA
0	0	0	4	2	NM_080054.2	CG1560-RA (mys), mRNA
0	0	0	3	10	NM_057920.3	CG3423-RA (SA), mRNA
0	0	0	3	8	NM_170407.2	CG11958-RA, transcript variant A (Cnx99A), mRNA

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