NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 16870R-1

Symbol Acyp Full Name Acylphosphatase

CG No CG16870 Old CG No CG16870

Synonyms AcPDro, CG16870, ACPDro, BG:DS00180.1, Acyp

Accession No (Link to NCBI) NM_078839.1

Inserted Chr. ll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees viable

Map Viewer

Reference
[Please submit your publication] Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Swarup S, Pradhan-Sundd T, Verheyen EM.
Genome-wide identification of phospho-regulators of Wnt signaling in Drosophila.
Development (2015) 142(8) 1502-15 [ PubMed ID = 25852200 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGACCCATAATGTCCATTCCTG

Primer Seq. 3'

aatctagaggtaccTGCTATCATGGTGGCTGGAAC

Predicted Fragment Size

IR fragment full Seq

0001 GACCCATAAT GTCCATTCCT GCGAATTCGA GGTATTTGGC CGCGTGCAGG GCGTCAACTT 
0061 CCGGCGACAC gCCCTGCGAA AGGCCAAGAC ACTGGGTCTT CGCGGCTGGT GCATGAACTC 
0121 CAGCAGGGGC ACCGTGAAGG GTTACATCGA AGGTCGTCCG GCCGAGATGG ATGTGATGAA 
0181 GGAGTGGCTC AGGACGACGG GCAGTCCGCT GTCCAGCATC GAGAANGTGG AGTTCAGTTC 
0241 TCAGCGTGAN GCGCGNATCG CTATGGCTAT GCCAACTTTC ATATCAAGCC CGATCCGCAC 
0301 GAGAATCGCC CAGTTCATGA AGGATTGGGC AGTAGTTCCA GCCACCATGA TAGCA

in silico PCR Fragment

0001 GACCCATAAT GTCCATTCCT GCGAATTCGA GGTATTTGGC CGCGTGCAGG GCGTCAACTT 
0061 CCGGCGACAC GCCCTGCGAA AGGCCAAGAC ACTGGGTCTT CGCGGCTGGT GCATGAACTC 
0121 CAGCAGGGGC ACCGTGAAGG GTTACATCGA AGGTCGTCCG GCCGAGATGG ATGTGATGAA 
0181 GGAGTGGCTC AGGACGACGG GCAGTCCGCT GTCCAGCATC GAGAAGGTGG AGTTCAGTTC 
0241 TCAGCGTGA- GCGCG-ATCG CTATGGCTAT GCCAACTTTC ATATCAAGCC CGATCCGCAC 
0301 GAGAATCGCC CAGTTCATGA AGGATTGGGC AGTAGTTCCA GCCACCATGA TAGCA

Assemble Data


16870R-1.IR_full       1   GACCCATAATGTCCATTCCTGCGAATTCGAGGTATTTGGCCGCGTGCAGGGCGTCAACTT 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
16870R-1.in silico     1   GACCCATAATGTCCATTCCTGCGAATTCGAGGTATTTGGCCGCGTGCAGGGCGTCAACTT 60


16870R-1.IR_full       61  CCGGCGACACGCCCTGCGAAAGGCCAAGACACTGGGTCTTCGCGGCTGGTGCATGAACTC 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
16870R-1.in silico     61  CCGGCGACACGCCCTGCGAAAGGCCAAGACACTGGGTCTTCGCGGCTGGTGCATGAACTC 120


16870R-1.IR_full       121 CAGCAGGGGCACCGTGAAGGGTTACATCGAAGGTCGTCCGGCCGAGATGGATGTGATGAA 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
16870R-1.in silico     121 CAGCAGGGGCACCGTGAAGGGTTACATCGAAGGTCGTCCGGCCGAGATGGATGTGATGAA 180


16870R-1.IR_full       181 GGAGTGGCTCAGGACGACGGGCAGTCCGCTGTCCAGCATCGAGAANGTGGAGTTCAGTTC 240
                           ||||||||||||||||||||||||||||||||||||||||||||| ||||||||||||||
16870R-1.in silico     181 GGAGTGGCTCAGGACGACGGGCAGTCCGCTGTCCAGCATCGAGAAGGTGGAGTTCAGTTC 240


16870R-1.IR_full       241 TCAGCGTGANGCGCGNATCGCTATGGCTATGCCAACTTTCATATCAAGCCCGATCCGCAC 300
                           ||||||||| ||||| ||||||||||||||||||||||||||||||||||||||||||||
16870R-1.in silico     241 TCAGCGTGA-GCGCG-ATCGCTATGGCTATGCCAACTTTCATATCAAGCCCGATCCGCAC 300


16870R-1.IR_full       301 GAGAATCGCCCAGTTCATGAAGGATTGGGCAGTAGTTCCAGCCACCATGATAGCA 355
                           |||||||||||||||||||||||||||||||||||||||||||||||||||||||
16870R-1.in silico     301 GAGAATCGCCCAGTTCATGAAGGATTGGGCAGTAGTTCCAGCCACCATGATAGCA 355

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	335	0	0	0	NM_078839.1	CG16870-RA (Acyp), mRNA
0	0	1	6	5	NM_132301.3	CG7065-RA (CG7065), mRNA
0	0	1	3	6	NM_001038795.1	CG8683-RA (CG8683), mRNA
0	0	1	2	7	NM_130545.2	CG11411-RA (fs(1)N), mRNA
0	0	0	6	8	NM_078520.2	CG2212-RA, transcript variant A (sws), mRNA
0	0	0	6	7	NM_167141.1	CG2212-RB, transcript variant B (sws), mRNA
0	0	0	4	10	NM_140428.2	CG6650-RA (CG6650), mRNA
0	0	0	4	4	NM_143701.2	CG6698-RA (NtR), mRNA
0	0	0	4	4	NM_078856.2	CG4838-RA (beat-Ic), mRNA
0	0	0	4	4	NM_142218.1	CG18522-RA (CG18522), mRNA
0	0	0	4	3	NM_164430.2	CG31666-RD, transcript variant D (CG31666), mRNA
0	0	0	4	3	NM_134766.3	CG31666-RA, transcript variant A (CG31666), mRNA
0	0	0	4	3	NM_078879.2	CG10363-RA (TepIV), mRNA
0	0	0	4	3	NM_164428.2	CG31666-RB, transcript variant B (CG31666), mRNA
0	0	0	4	3	NM_164429.1	CG31666-RC, transcript variant C (CG31666), mRNA
0	0	0	4	2	NM_143089.2	CG11849-RA (dan), mRNA
0	0	0	4	2	NM_130658.2	CG10260-RB (CG10260), mRNA
0	0	0	3	14	NM_175960.3	CG33196-RB (dp), mRNA
0	0	0	3	6	NM_079155.2	CG9102-RA (bab2), mRNA
0	0	0	3	5	NM_168290.1	CG5939-RB, transcript variant B (Prm), mRNA
0	0	0	3	5	NM_079258.2	CG5939-RA, transcript variant A (Prm), mRNA
0	0	0	3	3	NM_057257.3	CG7664-RA (crp), mRNA
0	0	0	3	3	NM_136527.1	CG14755-RA (CG14755), mRNA
0	0	0	3	2	NM_164988.2	CG12317-RB, transcript variant B (JhI-21), mRNA
0	0	0	3	2	NM_135698.2	CG12317-RA, transcript variant A (JhI-21), mRNA
0	0	0	3	2	NM_168085.1	CG11347-RC, transcript variant C (CG11347), mRNA
0	0	0	3	2	NM_139643.1	CG11347-RA, transcript variant A (CG11347), mRNA
0	0	0	3	2	NM_168083.1	CG11347-RB, transcript variant B (CG11347), mRNA
0	0	0	3	2	NM_168084.1	CG11347-RD, transcript variant D (CG11347), mRNA
0	0	0	3	2	NM_141520.2	CG9630-RA (CG9630), mRNA

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