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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
discarded
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
16863R-2 |
| Symbol |
CG16863 |
Full Name |
CG16863 |
| CG No |
CG16863 |
Old CG No |
CG16863 |
| Synonyms |
DS00797.6, BG:DS00797.6, CG16863 |
| Accession No (Link to NCBI) |
NM_135830.3 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgATGGAAGAACCTCTGCCGAGC |
| Primer Seq. 3' |
aatctagaggtaccTGATGATGAGCGCCCATGAAC |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 ATGGAAGAAC CTCTGCCGAG CGAGAGTCCG TTTCACAAGG ACATTGCCTT CTTTGTCTGC
0061 CGTGACCGCC AGTCATTGCA GATTGTGCAG GGCGAGGGAT TCCAGCACCT AGTGAAAGTA
0121 CTGTGCCCCA CCTACAGACC ACCGAGTGCG GCGAAACTGG AGGCCATTAT CTGCAGGGAG
0181 TCGGAAGCGC AGAGGTCCAA GCTCTGCCAG CAGTTGGCCG ACATCTCGAC ACTCAGCCTA
0241 AGTTGCTCGG TGCACACGAA TGCGGAGAGT CGAAGCTGGA TGGAGCTGGT GGCCCACTTT
0301 CACGAGGGGC GGCAACGAAT CTCCCGAACT CTTTNTGNGN TGCGCCTTCC CGATCTTTTC
0361 ACCTCCAACG ATCTGGTGGA TCGCATGGAC CAAGTGTGTC AGCGCTTCGA CATTCCAAAG
0421 TCAAGGATCT GCTGCGTGTC GACAAGGAGC AGTCAACTAC TGGAGGATGC GGTGACATCG
0481 TTCATGGGCG CTCATCATCA |
| in silico PCR Fragment |
0001 ATGGAAGAAC CTCTGCCGAG CGAGAGTCCG TTTCACAAGG ACATTGCCTT CTTTGTCTGC
0061 CGTGACCGCC AGTCATTGCA GATTGTGCAG GGCGAGGGAT TCCAGCACCT AGTGAAAGTA
0121 CTGTGCCCCA CCTACAGACC ACCGAGTGCG GCGAAACTGG AGGCCATTAT CTGCAGGGAG
0181 TCGGAAGCGC AGAGGTCCAA GCTCTGCCAG CAGTTGGCCG ACATCTCGAC ACTCAGCCTA
0241 AGTTGCTCGG TGCACACGAA TGCGGAGAGT CGAAGCTGGA TGGAGCTGGT GGCCCACTTT
0301 CACGAGGGGC GGCAACGAAT CTCCCGAACT CTTTCTGTGC TGCGCCTTCC CGATCTTTTC
0361 ACCTCCAACG ATCTGGTGGA TCGCATGGAC CAAGTGTGTC AGCGCTTCGA CATTCCAAAG
0421 TCAAGGATCT GCTGCGTGTC GACAAGGAGC AGTCAACTAC TGGAGGATGC GGTGACATCG
0481 TTCATGGGCG CTCATCATCA |
| Assemble Data |
16863R-2.IR_full 1 ATGGAAGAACCTCTGCCGAGCGAGAGTCCGTTTCACAAGGACATTGCCTTCTTTGTCTGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
16863R-2.in silico 1 ATGGAAGAACCTCTGCCGAGCGAGAGTCCGTTTCACAAGGACATTGCCTTCTTTGTCTGC 60
16863R-2.IR_full 61 CGTGACCGCCAGTCATTGCAGATTGTGCAGGGCGAGGGATTCCAGCACCTAGTGAAAGTA 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
16863R-2.in silico 61 CGTGACCGCCAGTCATTGCAGATTGTGCAGGGCGAGGGATTCCAGCACCTAGTGAAAGTA 120
16863R-2.IR_full 121 CTGTGCCCCACCTACAGACCACCGAGTGCGGCGAAACTGGAGGCCATTATCTGCAGGGAG 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
16863R-2.in silico 121 CTGTGCCCCACCTACAGACCACCGAGTGCGGCGAAACTGGAGGCCATTATCTGCAGGGAG 180
16863R-2.IR_full 181 TCGGAAGCGCAGAGGTCCAAGCTCTGCCAGCAGTTGGCCGACATCTCGACACTCAGCCTA 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
16863R-2.in silico 181 TCGGAAGCGCAGAGGTCCAAGCTCTGCCAGCAGTTGGCCGACATCTCGACACTCAGCCTA 240
16863R-2.IR_full 241 AGTTGCTCGGTGCACACGAATGCGGAGAGTCGAAGCTGGATGGAGCTGGTGGCCCACTTT 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
16863R-2.in silico 241 AGTTGCTCGGTGCACACGAATGCGGAGAGTCGAAGCTGGATGGAGCTGGTGGCCCACTTT 300
16863R-2.IR_full 301 CACGAGGGGCGGCAACGAATCTCCCGAACTCTTTNTGNGNTGCGCCTTCCCGATCTTTTC 360
|||||||||||||||||||||||||||||||||| || | ||||||||||||||||||||
16863R-2.in silico 301 CACGAGGGGCGGCAACGAATCTCCCGAACTCTTTCTGTGCTGCGCCTTCCCGATCTTTTC 360
16863R-2.IR_full 361 ACCTCCAACGATCTGGTGGATCGCATGGACCAAGTGTGTCAGCGCTTCGACATTCCAAAG 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
16863R-2.in silico 361 ACCTCCAACGATCTGGTGGATCGCATGGACCAAGTGTGTCAGCGCTTCGACATTCCAAAG 420
16863R-2.IR_full 421 TCAAGGATCTGCTGCGTGTCGACAAGGAGCAGTCAACTACTGGAGGATGCGGTGACATCG 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
16863R-2.in silico 421 TCAAGGATCTGCTGCGTGTCGACAAGGAGCAGTCAACTACTGGAGGATGCGGTGACATCG 480
16863R-2.IR_full 481 TTCATGGGCGCTCATCATCA 500
||||||||||||||||||||
16863R-2.in silico 481 TTCATGGGCGCTCATCATCA 500
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
2 |
NM_135830.3 |
CG16863-RA, transcript variant A (CG16863), mRNA |
| 100 |
482 |
0 |
0 |
2 |
NM_001042896.1 |
CG16863-RB, transcript variant B (CG16863), mRNA |
| 0 |
0 |
3 |
9 |
4 |
NM_168081.1 |
CG32250-RA (CG32250), mRNA |
| 0 |
0 |
2 |
5 |
3 |
NM_001043091.1 |
CG5748-RC, transcript variant C (Hsf), mRNA |
| 0 |
0 |
2 |
5 |
3 |
NM_057227.3 |
CG5748-RA, transcript variant A (Hsf), mRNA |
| 0 |
0 |
2 |
5 |
3 |
NM_001043090.1 |
CG5748-RD, transcript variant D (Hsf), mRNA |
| 0 |
0 |
2 |
5 |
3 |
NM_001043092.1 |
CG5748-RB, transcript variant B (Hsf), mRNA |
| 0 |
0 |
2 |
2 |
7 |
NM_140914.2 |
CG7749-RA, transcript variant A (fat2), mRNA |
| 0 |
0 |
2 |
2 |
7 |
NM_001031967.1 |
CG7749-RB, transcript variant B (fat2), mRNA |
| 0 |
0 |
1 |
5 |
5 |
NM_132504.1 |
CG11695-RA (CG11695), mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_133158.2 |
CG12204-RA (CG12204), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_143469.1 |
CG7837-RA (CG7837), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_136043.2 |
CG10413-RA (CG10413), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_137006.2 |
CG4663-RA (CG4663), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_140466.1 |
CG5295-RA (CG5295), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_165551.2 |
CG30496-RA (CG30496), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_057527.3 |
CG6122-RA (piwi), mRNA |
| 0 |
0 |
0 |
6 |
2 |
NM_132832.2 |
CG8191-RA (CG8191), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_144343.2 |
CG8250-RA (Alk), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_001038732.1 |
CG12598-RC, transcript variant C (Adar), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_130584.2 |
CG12598-RA, transcript variant A (Adar), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_166903.1 |
CG12598-RB, transcript variant B (Adar), mRNA |
| 0 |
0 |
0 |
3 |
10 |
NM_166696.1 |
CG9071-RB, transcript variant B (NaCP60E), mRNA |
| 0 |
0 |
0 |
3 |
9 |
NM_080255.2 |
CG6889-RA, transcript variant A (tara), mRNA |
| 0 |
0 |
0 |
3 |
9 |
NM_169709.1 |
CG6889-RB, transcript variant B (tara), mRNA |
| 0 |
0 |
0 |
3 |
7 |
NM_168587.1 |
CG17697-RB, transcript variant B (fz), mRNA |
| 0 |
0 |
0 |
3 |
7 |
NM_080073.2 |
CG17697-RA, transcript variant A (fz), mRNA |
| 0 |
0 |
0 |
3 |
6 |
NM_138986.3 |
CG1168-RA, transcript variant A (7B2), mRNA |
| 0 |
0 |
0 |
3 |
6 |
NM_001014608.1 |
CG1168-RB, transcript variant B (7B2), mRNA |
| 0 |
0 |
0 |
3 |
6 |
NM_079903.2 |
CG15319-RB (nej), mRNA |
|
| Wing Disc |
GFP
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Puc
 |
Caspase
 |
DAPI
 |
Merge
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Figure
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JNK signal
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Caspase-3 signal
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