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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 16785R-3 
 Symbol fz3  Full Name frizzled 3 
 CG No CG16785  Old CG No CG16785 
 Synonyms Dfz3, dfz3, DFz3, EG:34F3.6, CG16785, fz3, frizzled 3, Dfrizzled-3 
 Accession No (Link to NCBI) NM_080296.2 
 Inserted Chr. ll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]

Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624 [ PubMed ID = 21666802 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgAGGGCCTAACGGATTGCAGTG 
 Primer Seq. 3' 
aatctagaggtaccCAGCCAAGTCAGAGGTGGGAG 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 AGGGcCTAAC GGATTGCAGT GCCAGCCCAT CGCCGTGTCC GCCTGCCAAG GcCTCGGCTA 
0061 CAACATGACC GCACTGCCCA ACCTGGCAGG TCACACCAAT CAGCTGGAGG CTGAGCTCCA 
0121 GATTGCCAAA CTGGTGCCAC TAATAGAATC CGGCTGCTCG CGTCGCGCCC GCNTTTTTGC 
0181 TCTGCTCGTC TCTGTTTCCG CTGTGCACGC CGGATGTGCC ACGCCCCGTT GCCGCCTGCA 
0241 AATTGCTGTG CGAGACTGTT AGGGGCGAGT GTATGGAAAA CGCTCCGCCG GAACTGATGG 
0301 AGCTGTGGCC TTTCGTTCCT TAANCTGCGA CGGACTGCCG CAGCCGGAGA AGCACGAGTT 
0361 GTGCATGCAG ATTCCACAGG AGGTGGCTGT GCCAGGCGGG AGTCCAAGTG GTCCGCCCAC 
0421 CACTGGTTCA CCTGGCGTCG AAGATCACCC GCAGACGTAC CGCTTTTGGA AAAGCGGAGT 
0481 GTCTCCCACC TCTGACTTGG CTG 
 in silico PCR Fragment 
0001 AGGGCCTAAC GGATTGCAGT GCCAGCCCAT CGCCGTGTCC GCCTGCCAAG GCCTCGGCTA 
0061 CAACATGACC GCACTGCCCA ACCTGGCAGG TCACACCAAT CAGCTGGAGG CTGAGCTCCA 
0121 GATTGCCAAA CTGGTGCCAC TAATAGAATC CGGCTGCTCG CGTCGCGCCC GC-TTTTTGC 
0181 TCTGCTCGTC TCTGTTTCCG CTGTGCACGC CGGATGTGCC ACGCCCCGTT GCCGCCTGCA 
0241 AATTGCTGTG CGAGACTGTT AGGGGCGAGT GTATGGAAAA CGCTCCGCCG GAACTGATGG 
0301 AGCTGTGGCC -TTCGTTCCT TAA-CTGCGA CGGACTGCCG CAGCCGGAGA AGCACGAGTT 
0361 GTGCATGCAG ATTCCACAGG AGGTGGCTGT GCCAGGCGGG AGTCCAAGTG GTCCGCCCAC 
0421 CACTGGTTCA CCTGGCGTCG AAGATCACCC GCAGACGTAC CGCTTTTGGA AAAGCGGAGC 
0481 ATCTCCCACC TCTGACTTGG CTG 
 Assemble Data 

16785R-3.IR_full       1   AGGGCCTAACGGATTGCAGTGCCAGCCCATCGCCGTGTCCGCCTGCCAAGGCCTCGGCTA 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
16785R-3.in silico     1   AGGGCCTAACGGATTGCAGTGCCAGCCCATCGCCGTGTCCGCCTGCCAAGGCCTCGGCTA 60


16785R-3.IR_full       61  CAACATGACCGCACTGCCCAACCTGGCAGGTCACACCAATCAGCTGGAGGCTGAGCTCCA 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
16785R-3.in silico     61  CAACATGACCGCACTGCCCAACCTGGCAGGTCACACCAATCAGCTGGAGGCTGAGCTCCA 120


16785R-3.IR_full       121 GATTGCCAAACTGGTGCCACTAATAGAATCCGGCTGCTCGCGTCGCGCCCGCNTTTTTGC 180
                           |||||||||||||||||||||||||||||||||||||||||||||||||||| |||||||
16785R-3.in silico     121 GATTGCCAAACTGGTGCCACTAATAGAATCCGGCTGCTCGCGTCGCGCCCGC-TTTTTGC 180


16785R-3.IR_full       181 TCTGCTCGTCTCTGTTTCCGCTGTGCACGCCGGATGTGCCACGCCCCGTTGCCGCCTGCA 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
16785R-3.in silico     181 TCTGCTCGTCTCTGTTTCCGCTGTGCACGCCGGATGTGCCACGCCCCGTTGCCGCCTGCA 240


16785R-3.IR_full       241 AATTGCTGTGCGAGACTGTTAGGGGCGAGTGTATGGAAAACGCTCCGCCGGAACTGATGG 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
16785R-3.in silico     241 AATTGCTGTGCGAGACTGTTAGGGGCGAGTGTATGGAAAACGCTCCGCCGGAACTGATGG 300


16785R-3.IR_full       301 AGCTGTGGCCTTTCGTTCCTTAANCTGCGACGGACTGCCGCAGCCGGAGAAGCACGAGTT 360
                           |||||||||| |||||||||||| ||||||||||||||||||||||||||||||||||||
16785R-3.in silico     301 AGCTGTGGCC-TTCGTTCCTTAA-CTGCGACGGACTGCCGCAGCCGGAGAAGCACGAGTT 360


16785R-3.IR_full       361 GTGCATGCAGATTCCACAGGAGGTGGCTGTGCCAGGCGGGAGTCCAAGTGGTCCGCCCAC 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
16785R-3.in silico     361 GTGCATGCAGATTCCACAGGAGGTGGCTGTGCCAGGCGGGAGTCCAAGTGGTCCGCCCAC 420


16785R-3.IR_full       421 CACTGGTTCACCTGGCGTCGAAGATCACCCGCAGACGTACCGCTTTTGGAAAAGCGGAGT 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| 
16785R-3.in silico     421 CACTGGTTCACCTGGCGTCGAAGATCACCCGCAGACGTACCGCTTTTGGAAAAGCGGAGC 480


16785R-3.IR_full       481 GTCTCCCACCTCTGACTTGGCTG 503
                            ||||||||||||||||||||||
16785R-3.in silico     481 ATCTCCCACCTCTGACTTGGCTG 503



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  2  NM_080296.2  CG16785-RA (fz3), mRNA 
0   0  4  2  2  NM_079019.2  CG10122-RA (RpI1), mRNA 
0   0  3  2  2  NM_170006.2  CG31169-RB, transcript variant B (CG31169), mRNA 
0   0  3  2  2  NM_170005.1  CG31169-RA, transcript variant A (CG31169), mRNA 
0   0  2  3  2  NM_079435.2  CG9262-RA (Shal), mRNA 
0   0  2  2  6  NM_206691.2  CG1817-RA, transcript variant A (Ptp10D), mRNA 
0   0  2  2  6  NM_206690.1  CG1817-RD, transcript variant D (Ptp10D), mRNA 
0   0  2  2  6  NM_167292.2  CG1817-RB, transcript variant B (Ptp10D), mRNA 
0   0  2  2  2  NM_130576.2  CG3740-RA (CG3740), mRNA 
0   0  2  2  2  NM_057797.2  CG10387-RA (tos), mRNA 
0   0  1  2  6  NM_001043070.1  CG34146-RA (brp), mRNA 
0   0  1  2  4  NM_206506.1  CG7467-RC, transcript variant C (osa), mRNA 
0   0  1  2  4  NM_079668.2  CG7467-RB, transcript variant B (osa), mRNA 
0   0  1  2  4  NM_169775.1  CG7467-RA, transcript variant A (osa), mRNA 
0   0  0  7  2  NM_078913.1  CG12841-RA (Tsp42Ek), mRNA 
0   0  0  6  9  NM_136092.1  CG10470-RA (CG10470), mRNA 
0   0  0  6  5  NM_138171.1  CG17142-RA, transcript variant A (pyx), mRNA 
0   0  0  5  7  NM_132967.1  CG5004-RA (CG5004), mRNA 
0   0  0  4  8  NM_057724.3  CG18000-RI, transcript variant I (sw), mRNA 
0   0  0  4  7  NM_206796.1  CG33499-RA (Sdic:CG33499), mRNA 
0   0  0  4  7  NM_167693.2  CG32823-RB (Sdic:CG32823), mRNA 
0   0  0  4  7  NM_079931.3  CG9580-RA (Sdic:CG9580), mRNA 
0   0  0  4  7  NM_206793.1  CG33497-RA (Sdic:CG33497), mRNA 
0   0  0  4  5  NM_165445.2  CG8376-RA, transcript variant A (ap), mRNA 
0   0  0  4  5  NM_166872.1  CG3638-RD, transcript variant D (CG3638), mRNA 
0   0  0  4  5  NM_130541.3  CG3638-RC, transcript variant C (CG3638), mRNA 
0   0  0  4  5  NM_166874.1  CG3638-RA, transcript variant A (CG3638), mRNA 
0   0  0  4  5  NM_141118.3  CG7470-RA (CG7470), mRNA 
0   0  0  4  4  NM_166873.1  CG3638-RB, transcript variant B (CG3638), mRNA 
0   0  0  4  4  NM_168218.1  CG32372-RA (CG32372), mRNA 

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