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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 1624R-2 
 Symbol dpld  Full Name dappled 
 CG No CG1624  Old CG No CG1624 
 Synonyms lin-41, CG1624, l(2)k08815-3, dpld 
 Accession No (Link to NCBI) NM_165533.1 
 Inserted Chr. ll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgCAGTGTGAGCAGCAATGCCAG 
 Primer Seq. 3' 
aatctagaggtaccGGCAGTAGTCACATACGTAGC 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 CAGTGTGAGC AGCAATGcCA GCAACTCCTC CGAGCGACTG CTGGCGGGCA TCCTGGAGAG 
0061 CTTTCCCGCC TgGGACCTCA ACGTGGGACT ANCTGCCCAA CGTGGGcCAG AGCTCACCAC 
0121 CACGCGCAGA CTTCTTTATC AATAACTTCC TGGGCGGCCT GGACACGCAT GGCGACTTCA 
0181 GCATTGGACC CATTGGCAGC GGTGCCCGCA GCAATCCGAA GATGTCGCCA GAGTCATCTA 
0241 ACAACTCGAG CATCAGCTGC GGTTGGTGCG AGGTTAGTGC TTCAATACGC TGCCTGGAGT 
0301 GCAACGAGTT CATGTGCAAC GACTGCCTAA GGGAGCATCG CAACAGTCCG CTGTCCTCCA 
0361 ACCACTCGAT CGTGTCCTTG CCCACGCCCA TTGGAGCCTC GcCCACGGGT GGCAGCTCGG 
0421 TAAATGCACA NGANCTCcgN CCAGCGGCAA CTTTATCTGC GACATACACA ACGAGATGTT 
0481 GCGCTACGTA TGTGACTATC TNCc 
 in silico PCR Fragment 
0001 CAGTGTGAGC AGCAATGCCA GCAACTCCTC CGAGCGACTG CTGGCGGGCA TCCTGGAGAG 
0061 CTTTCCCGCC TGGGACCTCA ACGTGGGACT A-CTGCCCAA CGTGGGCCAG AGCTCACCAC 
0121 CACGCGCAGA CTTCTTTATC AATAACTTCC TGGGCGGCCT GGACACGCAT GGCGACTTCA 
0181 GCATTGGACC CATTGGCAGC GGTGCCCGCA GCAATCCGAA GATGTCGCCA GAGTCATCTA 
0241 ACAACTCGAG CATCAGCTGC GGTTGGTGCG AGGTTAGTGC TTCAATACGC TGCCTGGAGT 
0301 GCAACGAGTT CATGTGCAAC GACTGCCTAA GGGAGCATCG CAACAGTCCG CTGTCCTCCA 
0361 ACCACTCGAT CGTGTCCTTG CCCACGCCCA TTGGAGCCTC GCCCACGGGT GGCAGCTCGG 
0421 TAAATGCACA -GA-CTCCGC CCAGCGGCAA CTTTATCTGC GACATACACA ACGAGATGTT 
0481 GCGCTACGTA TGTGACTA-C TGCC 
 Assemble Data 

1624R-2.IR_full       1   CAGTGTGAGCAGCAATGCCAGCAACTCCTCCGAGCGACTGCTGGCGGGCATCCTGGAGAG 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1624R-2.in silico     1   CAGTGTGAGCAGCAATGCCAGCAACTCCTCCGAGCGACTGCTGGCGGGCATCCTGGAGAG 60


1624R-2.IR_full       61  CTTTCCCGCCTGGGACCTCAACGTGGGACTANCTGCCCAACGTGGGCCAGAGCTCACCAC 120
                          ||||||||||||||||||||||||||||||| ||||||||||||||||||||||||||||
1624R-2.in silico     61  CTTTCCCGCCTGGGACCTCAACGTGGGACTA-CTGCCCAACGTGGGCCAGAGCTCACCAC 120


1624R-2.IR_full       121 CACGCGCAGACTTCTTTATCAATAACTTCCTGGGCGGCCTGGACACGCATGGCGACTTCA 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1624R-2.in silico     121 CACGCGCAGACTTCTTTATCAATAACTTCCTGGGCGGCCTGGACACGCATGGCGACTTCA 180


1624R-2.IR_full       181 GCATTGGACCCATTGGCAGCGGTGCCCGCAGCAATCCGAAGATGTCGCCAGAGTCATCTA 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1624R-2.in silico     181 GCATTGGACCCATTGGCAGCGGTGCCCGCAGCAATCCGAAGATGTCGCCAGAGTCATCTA 240


1624R-2.IR_full       241 ACAACTCGAGCATCAGCTGCGGTTGGTGCGAGGTTAGTGCTTCAATACGCTGCCTGGAGT 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1624R-2.in silico     241 ACAACTCGAGCATCAGCTGCGGTTGGTGCGAGGTTAGTGCTTCAATACGCTGCCTGGAGT 300


1624R-2.IR_full       301 GCAACGAGTTCATGTGCAACGACTGCCTAAGGGAGCATCGCAACAGTCCGCTGTCCTCCA 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1624R-2.in silico     301 GCAACGAGTTCATGTGCAACGACTGCCTAAGGGAGCATCGCAACAGTCCGCTGTCCTCCA 360


1624R-2.IR_full       361 ACCACTCGATCGTGTCCTTGCCCACGCCCATTGGAGCCTCGCCCACGGGTGGCAGCTCGG 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1624R-2.in silico     361 ACCACTCGATCGTGTCCTTGCCCACGCCCATTGGAGCCTCGCCCACGGGTGGCAGCTCGG 420


1624R-2.IR_full       421 TAAATGCACANGANCTCCGNCCAGCGGCAACTTTATCTGCGACATACACAACGAGATGTT 480
                          |||||||||| || ||||| ||||||||||||||||||||||||||||||||||||||||
1624R-2.in silico     421 TAAATGCACA-GA-CTCCGCCCAGCGGCAACTTTATCTGCGACATACACAACGAGATGTT 480


1624R-2.IR_full       481 GCGCTACGTATGTGACTATCTNCC 504
                          |||||||||||||||||| || ||
1624R-2.in silico     481 GCGCTACGTATGTGACTA-CTGCC 504



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_080033.2  CG1624-RC, transcript variant C (dpld), mRNA 
100   482  0  0  0  NM_165534.1  CG1624-RB, transcript variant B (dpld), mRNA 
100   482  0  0  0  NM_165533.1  CG1624-RA, transcript variant A (dpld), mRNA 
0   0  2  5  3  NM_080208.2  CG8822-RA (PpD6), mRNA 
0   0  2  3  2  NM_057739.3  CG3969-RB, transcript variant B (PR2), mRNA 
0   0  2  3  2  NM_057738.3  CG3969-RA, transcript variant A (PR2), mRNA 
0   0  2  2  7  NM_079470.2  CG10564-RA (Ac78C), mRNA 
0   0  2  2  5  NM_168079.1  CG15013-RC, transcript variant C (dyl), mRNA 
0   0  2  2  5  NM_139633.2  CG15013-RA, transcript variant A (dyl), mRNA 
0   0  2  2  5  NM_168078.1  CG15013-RB, transcript variant B (dyl), mRNA 
0   0  2  2  4  NM_143259.1  CG5521-RA (CG5521), mRNA 
0   0  2  2  3  NM_135896.3  CG4168-RA (CG4168), mRNA 
0   0  2  1  3  NM_168449.1  CG32076-RA (Alg10), mRNA 
0   0  1  5  4  NM_166584.1  CG3082-RD, transcript variant D (l(2)k09913), mRNA 
0   0  1  5  4  NM_166582.1  CG3082-RA, transcript variant A (l(2)k09913), mRNA 
0   0  1  5  4  NM_137907.2  CG3082-RC, transcript variant C (l(2)k09913), mRNA 
0   0  1  5  4  NM_166583.1  CG3082-RB, transcript variant B (l(2)k09913), mRNA 
0   0  1  3  6  NM_057690.2  CG8084-RA (ana), mRNA 
0   0  1  3  3  NM_132531.1  CG11802-RA (CG11802), mRNA 
0   0  1  3  3  NM_165098.1  CG31832-RA (CG31832), mRNA 
0   0  1  2  18  NM_143225.2  CG6490-RA (CG6490), mRNA 
0   0  1  2  5  NM_141673.4  CG31352-RA (CG31352), mRNA 
0   0  1  2  5  NM_169302.1  CG31332-RA, transcript variant A (unc-115), mRNA 
0   0  1  2  5  NM_169303.2  CG31332-RB, transcript variant B (unc-115), mRNA 
0   0  1  2  5  NM_169300.2  CG31332-RD, transcript variant D (unc-115), mRNA 
0   0  1  2  5  NM_169301.2  CG31332-RC, transcript variant C (unc-115), mRNA 
0   0  1  2  3  NM_080314.2  CG8590-RA (Klp3A), mRNA 
0   0  1  2  3  NM_142474.1  CG14304-RA (CG14304), mRNA 
0   0  1  2  2  NM_080138.2  CG1618-RA (comt), mRNA 
0   0  1  2  2  NM_132994.1  CG12992-RA (CG12992), mRNA 
 Wing Disc
GFP
 Puc
 Caspase
DAPI
 Merge
 Figure
JNK signal
N
 
DB
+- 
DC
++ 
DM
+- 
VM
 
VB
 
Caspase-3 signal
N
 
DB
++ 
DC
++ 
DM
+ 
VM
+ 
VB
 
Dcompartment   Penetrance
    - 

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