NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 1603R-1

Symbol CG1603 Full Name CG1603

CG No CG1603 Old CG No CG1603

Synonyms CG1603

Accession No (Link to NCBI) NM_136446.2

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees semi-lethal

Map Viewer

Reference
[Please submit your publication] Ohhara Y, Nakamura A, Kato Y, Yamakawa-Kobayashi K.
Chaperonin TRiC/CCT supports mitotic exit and entry into endocycle in Drosophila.
PLoS Genet (2019) 15(4) e1008121 [ PubMed ID = 31034473 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGTGCGGCAATGTGATGACCAA

Primer Seq. 3'

aatctagaggtaccGAGGTGCCAAGTCATCCTCAG

Predicted Fragment Size

IR fragment full Seq

0001 GTGCGGCAAT GTGATGACCA ACTCCAAGTA CGAGATGTTC CGGCTGAAAT GCCTCTACTG 
0061 CAGCATAGAG AGCGAGCTGA AGGATTGGGA GCTGTTCATC GTCCATGTGA AATCGGCGCA 
0121 CTACTGCGAA GACGAGGACG TTAGGATTAA TGAAATCAAA GGGGATACCG AGGAGCTATA 
0181 CAGCGTCGTT GACGCGGCGG ACCCAGCCAT CGCCTATGGG CCGGATGAGT TCTTCGAGGT 
0241 GATCGAAAAC AGCAATGGGG CCGATCAATG GATGGAAGCT GATAGCAAGG ATATCCAGTA 
0301 CGAGGAAGAC GCCACCGCTT GGTCTGCAGC TACAAACAAT TCCTGGGAGT TCGGTCCAGG 
0361 CGGCAGCTCT TTGGAACTTC CAGCCGGAAC GGAAGTCACA CAAATGGACG AGTCTTCCCA 
0421 AAATCACATA CCCCTAAACA CAGACGATGA TGATGTTGAT TGTAGTGACT TCTTTATGTC 
0481 TGAGGATGAC TTGGCACCTC

in silico PCR Fragment

0001 GTGCGGCAAT GTGATGACCA ACTCCAAGTA CGAGATGTTC CGGCTGAAAT GCCTCTACTG 
0061 CAGCATAGAG AGCGAGCTGA AGGATTGGGA GCTGTTCATC GTCCATGTGA AATCGGCGCA 
0121 CTACTGCGAA GACGAGGACG TTAGGATTAA TGAAATCAAA GAGGATACCG AGGAGCTATA 
0181 CAGCGTCGTT GACGCGGCGG ACCCAGCCAT CGCCTATGGG CCGGATGAGT TCTTCGAGGT 
0241 GATCGAAAAC AGCAATGGGG TCGATCAATG GATGGAAGCT GATAGCAAGG ATATCCAGTA 
0301 CGAGGAAGAC GCCACCGCTT GGTCTGCAGC TACAAACAAT TCCTGGGAGT TCGGTCCAGG 
0361 CGGCAGCTCT TTGGAACTTC CAGCCGGAAA GGAAGTCACA CAAATGGACG AGTCTTCCCA 
0421 AAATCACATA CCCCTAAACA CAGACGATGA TGATGTTGAT TGTAGTGACT TCTTTATGTC 
0481 TGAGGATGAC TTGGCACCTC

Assemble Data


1603R-1.IR_full       1   GTGCGGCAATGTGATGACCAACTCCAAGTACGAGATGTTCCGGCTGAAATGCCTCTACTG 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1603R-1.in silico     1   GTGCGGCAATGTGATGACCAACTCCAAGTACGAGATGTTCCGGCTGAAATGCCTCTACTG 60


1603R-1.IR_full       61  CAGCATAGAGAGCGAGCTGAAGGATTGGGAGCTGTTCATCGTCCATGTGAAATCGGCGCA 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1603R-1.in silico     61  CAGCATAGAGAGCGAGCTGAAGGATTGGGAGCTGTTCATCGTCCATGTGAAATCGGCGCA 120


1603R-1.IR_full       121 CTACTGCGAAGACGAGGACGTTAGGATTAATGAAATCAAAGGGGATACCGAGGAGCTATA 180
                          ||||||||||||||||||||||||||||||||||||||||| ||||||||||||||||||
1603R-1.in silico     121 CTACTGCGAAGACGAGGACGTTAGGATTAATGAAATCAAAGAGGATACCGAGGAGCTATA 180


1603R-1.IR_full       181 CAGCGTCGTTGACGCGGCGGACCCAGCCATCGCCTATGGGCCGGATGAGTTCTTCGAGGT 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1603R-1.in silico     181 CAGCGTCGTTGACGCGGCGGACCCAGCCATCGCCTATGGGCCGGATGAGTTCTTCGAGGT 240


1603R-1.IR_full       241 GATCGAAAACAGCAATGGGGCCGATCAATGGATGGAAGCTGATAGCAAGGATATCCAGTA 300
                          |||||||||||||||||||| |||||||||||||||||||||||||||||||||||||||
1603R-1.in silico     241 GATCGAAAACAGCAATGGGGTCGATCAATGGATGGAAGCTGATAGCAAGGATATCCAGTA 300


1603R-1.IR_full       301 CGAGGAAGACGCCACCGCTTGGTCTGCAGCTACAAACAATTCCTGGGAGTTCGGTCCAGG 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1603R-1.in silico     301 CGAGGAAGACGCCACCGCTTGGTCTGCAGCTACAAACAATTCCTGGGAGTTCGGTCCAGG 360


1603R-1.IR_full       361 CGGCAGCTCTTTGGAACTTCCAGCCGGAACGGAAGTCACACAAATGGACGAGTCTTCCCA 420
                          ||||||||||||||||||||||||||||| ||||||||||||||||||||||||||||||
1603R-1.in silico     361 CGGCAGCTCTTTGGAACTTCCAGCCGGAAAGGAAGTCACACAAATGGACGAGTCTTCCCA 420


1603R-1.IR_full       421 AAATCACATACCCCTAAACACAGACGATGATGATGTTGATTGTAGTGACTTCTTTATGTC 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1603R-1.in silico     421 AAATCACATACCCCTAAACACAGACGATGATGATGTTGATTGTAGTGACTTCTTTATGTC 480


1603R-1.IR_full       481 TGAGGATGACTTGGCACCTC 500
                          ||||||||||||||||||||
1603R-1.in silico     481 TGAGGATGACTTGGCACCTC 500

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_136446.2	CG1603-RA (CG1603), mRNA
0	0	4	28	43	NM_132390.1	CG15306-RA (CG15306), mRNA
0	0	2	6	6	NM_206363.2	CG17839-RB, transcript variant B (CG17839), mRNA
0	0	2	5	11	NM_001043114.1	CG33484-RD, transcript variant D (zormin), mRNA
0	0	2	5	10	NM_001043112.1	CG33484-RB, transcript variant B (zormin), mRNA
0	0	2	5	7	NM_001043113.1	CG33484-RC, transcript variant C (zormin), mRNA
0	0	2	5	7	NM_206240.1	CG33484-RA, transcript variant A (zormin), mRNA
0	0	2	3	2	NM_132692.2	CG10992-RA (CG10992), mRNA
0	0	1	3	5	NM_166874.1	CG3638-RA, transcript variant A (CG3638), mRNA
0	0	1	3	5	NM_142706.2	CG3308-RA (CG3308), mRNA
0	0	1	3	5	NM_130541.3	CG3638-RC, transcript variant C (CG3638), mRNA
0	0	1	3	5	NM_166872.1	CG3638-RD, transcript variant D (CG3638), mRNA
0	0	1	3	5	NM_166873.1	CG3638-RB, transcript variant B (CG3638), mRNA
0	0	1	3	5	NM_142705.1	CG5919-RA (CG5919), mRNA
0	0	1	3	3	NM_137736.1	CG15666-RA (CG15666), mRNA
0	0	1	3	2	NM_143314.2	CG5634-RA (dsd), mRNA
0	0	1	2	6	NM_136069.2	CG10600-RA (CG10600), mRNA
0	0	1	2	6	NM_142983.2	CG6356-RA (CG6356), mRNA
0	0	1	2	5	NM_132301.3	CG7065-RA (CG7065), mRNA
0	0	1	2	4	NM_138205.1	CG3386-RA (CG3386), mRNA
0	0	1	2	2	NM_132260.2	CG12113-RA (l(1)G0095), mRNA
0	0	1	2	2	NM_136030.1	CG15157-RA (CG15157), mRNA
0	0	1	2	2	NM_142603.2	CG4854-RA (CG4854), mRNA
0	0	0	5	13	NM_168401.1	CG6611-RC, transcript variant C (ect), mRNA
0	0	0	5	4	NM_136251.2	CG9246-RA (CG9246), mRNA
0	0	0	5	3	NM_135247.2	CG9138-RA (SP1070), mRNA
0	0	0	4	8	NM_168400.1	CG6611-RB, transcript variant B (ect), mRNA
0	0	0	4	8	NM_079967.4	CG6611-RA, transcript variant A (ect), mRNA
0	0	0	4	5	NM_135887.2	CG3491-RA (CG3491), mRNA
0	0	0	4	5	NM_138175.2	CG7020-RA (DIP2), mRNA

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