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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
discarded
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
15773R-4 |
| Symbol |
CG15773 |
Full Name |
CG15773 |
| CG No |
CG15773 |
Old CG No |
CG15773 |
| Synonyms |
CG15773 |
| Accession No (Link to NCBI) |
NM_132023.1 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624
[
PubMed ID = 21666802
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGAAAGCGAGCACTGGATTCCC |
| Primer Seq. 3' |
aatctagaggtaccCTTAAGGCAGACGTGCGGACA |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 GAAAGCGAGC ACTGGATTCC CAACTGCCGC TCCTTGCGCT GGGTGGCCTA ATCATTATGG
0061 GATTGACCAT CAGTCGATTC CCTGGAGTGC TATGCCTGCG ACTCCGCCGA AGATTCGGAG
0121 TGTGCCACCC GACCGGGTCA GCAGCTGGAA GTGGAGGAGT GTCAGCAGAC GGGCGACGAG
0181 TGTGTCACCT CCATATCGGC GGGATTAACG CGACGCGGCT GCCTGGCGCG GCTGTATCCT
0241 AATGGCTATT GTGCCGCTCC ATGCGACCGG TGCAATACGA GCCTGTGCAA TCGGCACGTT
0301 TATCCTGCGG ATCGATTGCG GTGCTATCAG TGCAGCGGAT CGGATTGCAT CGATGTGGCC
0361 AGTcGGCCGC AATACCTGCT GCCGTGTCCG GTTTACCAGG AGGATGACAG GTGCTACACG
0421 AACGTAGTCC ACTTATCCAA CACCCTGCGC GGCTGTGAGC ACACCAATTT GCCGACAACC
0481 TGTCCGCACG TCTGCCTTAA G |
| in silico PCR Fragment |
0001 GAAAGCGAGC ACTGGATTCC CAACTGCCGC TCCTTGCGCT GGGTGGCCTA ATCATTATGG
0061 GATTGGCCAT -AGTCGATTC CCTGGAGTGT TATGCCTGCG ACTCCGCCGA AGATTCGGAG
0121 TGTGCCACCC GACCGGGTCA GCAGCTGGAA GTGGAGGAGT GTCAGCAGAC GGGCGACGAG
0181 TGTGTCACCT CCATATCGGC GGGATTAACG CGACGCGGCT GCCTGGCGCG GCTGTATCCT
0241 AATGGCTATT GTGCCGCTCC ATGCGACCGG TGCAATACGA GCCTGTGCAA TCGGCACGTT
0301 TATCCTGCGG ATCGATTGCG GTGCTATCAG TGCAGCGGAT CGGATTGCAT CGATGTGGCC
0361 AGTCGGCCGC AATACCTGCT GCCGTGTCCG GTTTACCAGG AGGATGACAG GTGCTACACG
0421 AACGTAGTCC ACTTATCCAA CACCCTGCGC GGCTGTGAGC ACACCAATTT GCCGACAACC
0481 TGTCCGCACG TCTGCCTTAA G |
| Assemble Data |
15773R-4.IR_full 1 GAAAGCGAGCACTGGATTCCCAACTGCCGCTCCTTGCGCTGGGTGGCCTAATCATTATGG 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15773R-4.in silico 1 GAAAGCGAGCACTGGATTCCCAACTGCCGCTCCTTGCGCTGGGTGGCCTAATCATTATGG 60
15773R-4.IR_full 61 GATTGACCATCAGTCGATTCCCTGGAGTGCTATGCCTGCGACTCCGCCGAAGATTCGGAG 120
||||| |||| |||||||||||||||||| ||||||||||||||||||||||||||||||
15773R-4.in silico 61 GATTGGCCAT-AGTCGATTCCCTGGAGTGTTATGCCTGCGACTCCGCCGAAGATTCGGAG 120
15773R-4.IR_full 121 TGTGCCACCCGACCGGGTCAGCAGCTGGAAGTGGAGGAGTGTCAGCAGACGGGCGACGAG 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15773R-4.in silico 121 TGTGCCACCCGACCGGGTCAGCAGCTGGAAGTGGAGGAGTGTCAGCAGACGGGCGACGAG 180
15773R-4.IR_full 181 TGTGTCACCTCCATATCGGCGGGATTAACGCGACGCGGCTGCCTGGCGCGGCTGTATCCT 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15773R-4.in silico 181 TGTGTCACCTCCATATCGGCGGGATTAACGCGACGCGGCTGCCTGGCGCGGCTGTATCCT 240
15773R-4.IR_full 241 AATGGCTATTGTGCCGCTCCATGCGACCGGTGCAATACGAGCCTGTGCAATCGGCACGTT 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15773R-4.in silico 241 AATGGCTATTGTGCCGCTCCATGCGACCGGTGCAATACGAGCCTGTGCAATCGGCACGTT 300
15773R-4.IR_full 301 TATCCTGCGGATCGATTGCGGTGCTATCAGTGCAGCGGATCGGATTGCATCGATGTGGCC 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15773R-4.in silico 301 TATCCTGCGGATCGATTGCGGTGCTATCAGTGCAGCGGATCGGATTGCATCGATGTGGCC 360
15773R-4.IR_full 361 AGTCGGCCGCAATACCTGCTGCCGTGTCCGGTTTACCAGGAGGATGACAGGTGCTACACG 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15773R-4.in silico 361 AGTCGGCCGCAATACCTGCTGCCGTGTCCGGTTTACCAGGAGGATGACAGGTGCTACACG 420
15773R-4.IR_full 421 AACGTAGTCCACTTATCCAACACCCTGCGCGGCTGTGAGCACACCAATTTGCCGACAACC 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15773R-4.in silico 421 AACGTAGTCCACTTATCCAACACCCTGCGCGGCTGTGAGCACACCAATTTGCCGACAACC 480
15773R-4.IR_full 481 TGTCCGCACGTCTGCCTTAAG 501
|||||||||||||||||||||
15773R-4.in silico 481 TGTCCGCACGTCTGCCTTAAG 501
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
2 |
NM_132023.1 |
CG15773-RA (CG15773), mRNA |
| 0.41 |
2 |
3 |
2 |
4 |
NM_167841.1 |
CG32341-RA (CG32341), mRNA |
| 0 |
0 |
6 |
36 |
58 |
NM_167319.3 |
CG32656-RA (CG32656), mRNA |
| 0 |
0 |
3 |
3 |
3 |
NM_133143.1 |
CG8002-RA (rictor), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_001038909.1 |
CG33205-RI, transcript variant I (CG33205), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_079211.2 |
CG10583-RA (Sse), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_078711.2 |
CG1424-RA (mst), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_176126.2 |
CG2204-RF, transcript variant F (G-oalpha47A), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_176127.2 |
CG2204-RG, transcript variant G (G-oalpha47A), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_078960.4 |
CG2204-RA, transcript variant A (G-oalpha47A), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_206079.1 |
CG2204-RI, transcript variant I (G-oalpha47A), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_206080.1 |
CG2204-RH, transcript variant H (G-oalpha47A), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_176125.2 |
CG2204-RE, transcript variant E (G-oalpha47A), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_176124.2 |
CG2204-RD, transcript variant D (G-oalpha47A), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_165773.2 |
CG2204-RC, transcript variant C (G-oalpha47A), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_165772.3 |
CG2204-RB, transcript variant B (G-oalpha47A), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_167230.1 |
CG32683-RA (CG32683), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_134766.3 |
CG31666-RA, transcript variant A (CG31666), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_164428.2 |
CG31666-RB, transcript variant B (CG31666), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_164429.1 |
CG31666-RC, transcript variant C (CG31666), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_057311.4 |
CG6246-RA (nub), mRNA |
| 0 |
0 |
0 |
6 |
6 |
NM_132443.1 |
CG16922-RA (CG16922), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_141687.1 |
CG12946-RA, transcript variant A (CG12946), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_001043234.1 |
CG12946-RB, transcript variant B (CG12946), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_143428.2 |
CG14514-RA (Brd8), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_143754.2 |
CG18660-RC, transcript variant C (Nckx30C), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_164870.1 |
CG18660-RB, transcript variant B (Nckx30C), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_078844.2 |
CG3688-RA (l(2)35Bd), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_164869.1 |
CG18660-RA, transcript variant A (Nckx30C), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_169747.1 |
CG3937-RC, transcript variant C (cher), mRNA |
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| Wing Disc |
GFP
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Puc
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Caspase
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DAPI
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Merge
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Figure
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JNK signal
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Caspase-3 signal
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