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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
15658R-1 |
| Symbol |
CG15658 |
Full Name |
CG15658 |
| CG No |
CG15658 |
Old CG No |
CG15658 |
| Synonyms |
CT35842, CG15658 |
| Accession No (Link to NCBI) |
NM_137717.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
lethal |
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Richier B, Vijandi CM, Mackensen S, Salecker I.
Lapsyn controls branch extension and positioning of astrocyte-like glia in the Drosophila optic lobe.
Nat Commun (2017) 8(1) 317
[
PubMed ID = 28827667
]
[
RRC reference
]
Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340
[
PubMed ID = 25473846
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgTGGCAGCTGCTGACGTTCATC |
| Primer Seq. 3' |
aatctagaggtaccGGTAACTCCTGTAGCTCGCAA |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 TGGCAGCTGC TGACGTTCAT CGTGTGCCTC CAGTTGTTGC ATTCCGCCGG ATTCATCATT
0061 CAGTCGGAAG TGCGCAAGTG CACGTNACGG CCATATCGAT AAGCTGCTCC GGATTCGTTG
0121 CTACGACTTG GACCTCAAGG AGGTGCCACA GAACCTCAAG AGCTCGNTGG AGGTTCTGGA
0181 TCTATCGCAC AATCGCATCA GGAAGCTGAA AACTAGCTCG TTTCAGCGAT ACACGGACAT
0241 TAAGTTTCTG ATGCTCTATG ATAACATGAT CTTATCGGTG GAGGTGGGAA CCTTCGAGCC
0301 ACTCACCTCG CTCCAGGAAA TTGATCTTTC GAATAATGGA CTGACAACGA TACCGCTGGA
0361 GTTGTTNCCA GCTGCCGCGA CTGAGAAACC TATACATCGA TAGCAATGAG CTAACATCCC
0421 TCAATCTTCA GGCACTGGAG AAGCCCATTC GTGCTCCACT GGAGTACCTG AACGTGGCAG
0481 GTTGCGAGCT ACAGGAGTTA CC |
| in silico PCR Fragment |
0001 TGGCAGCTGC TGACGTTCAT CGTGTGCCTC CAGTTGTTGC ATTCCGCCGG ATTCATCATT
0061 CAGTCGGAAG TGCGCAAGTG CACGT-ACGG CCATATCGAT AAGCTGCTCC GGATTCGTTG
0121 CTACGACTTG GACCTCAAGG AGGTGCCACA GAACCTCAAG AGTTCGGTGG AGGTTCTGGA
0181 TCTATCGCAC AATCGCATCA GGAAGCTGAA AACTAGCTCG TTTCAGCGAT ACACGGACAT
0241 TAAGTTTCTG ATGCTCTATG ATAACATGAT CTTATCGGTG GAGGTGGGAA CCTTCGAGCC
0301 ACTCACCTCG CTCCAGGAAA TTGATCTTTC GAATAATGGA CTGACAACGA TACCGCTGGA
0361 GTTGTT-CCA GCTGCCGCGA CTGAGAAACC TATACATCGA TAGCAATGAG CTAACATCCC
0421 TCAATCTTCA GGCACTGGAG AAGCCCATTC GTGCTCCACT GGAGTACCTG AACGTGGCAG
0481 GTTGCGAGCT ACAGGAGTTA CC |
| Assemble Data |
15658R-1.IR_full 1 TGGCAGCTGCTGACGTTCATCGTGTGCCTCCAGTTGTTGCATTCCGCCGGATTCATCATT 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15658R-1.in silico 1 TGGCAGCTGCTGACGTTCATCGTGTGCCTCCAGTTGTTGCATTCCGCCGGATTCATCATT 60
15658R-1.IR_full 61 CAGTCGGAAGTGCGCAAGTGCACGTNACGGCCATATCGATAAGCTGCTCCGGATTCGTTG 120
||||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||
15658R-1.in silico 61 CAGTCGGAAGTGCGCAAGTGCACGT-ACGGCCATATCGATAAGCTGCTCCGGATTCGTTG 120
15658R-1.IR_full 121 CTACGACTTGGACCTCAAGGAGGTGCCACAGAACCTCAAGAGCTCGNTGGAGGTTCTGGA 180
|||||||||||||||||||||||||||||||||||||||||| ||| |||||||||||||
15658R-1.in silico 121 CTACGACTTGGACCTCAAGGAGGTGCCACAGAACCTCAAGAGTTCGGTGGAGGTTCTGGA 180
15658R-1.IR_full 181 TCTATCGCACAATCGCATCAGGAAGCTGAAAACTAGCTCGTTTCAGCGATACACGGACAT 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15658R-1.in silico 181 TCTATCGCACAATCGCATCAGGAAGCTGAAAACTAGCTCGTTTCAGCGATACACGGACAT 240
15658R-1.IR_full 241 TAAGTTTCTGATGCTCTATGATAACATGATCTTATCGGTGGAGGTGGGAACCTTCGAGCC 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15658R-1.in silico 241 TAAGTTTCTGATGCTCTATGATAACATGATCTTATCGGTGGAGGTGGGAACCTTCGAGCC 300
15658R-1.IR_full 301 ACTCACCTCGCTCCAGGAAATTGATCTTTCGAATAATGGACTGACAACGATACCGCTGGA 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15658R-1.in silico 301 ACTCACCTCGCTCCAGGAAATTGATCTTTCGAATAATGGACTGACAACGATACCGCTGGA 360
15658R-1.IR_full 361 GTTGTTNCCAGCTGCCGCGACTGAGAAACCTATACATCGATAGCAATGAGCTAACATCCC 420
|||||| |||||||||||||||||||||||||||||||||||||||||||||||||||||
15658R-1.in silico 361 GTTGTT-CCAGCTGCCGCGACTGAGAAACCTATACATCGATAGCAATGAGCTAACATCCC 420
15658R-1.IR_full 421 TCAATCTTCAGGCACTGGAGAAGCCCATTCGTGCTCCACTGGAGTACCTGAACGTGGCAG 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15658R-1.in silico 421 TCAATCTTCAGGCACTGGAGAAGCCCATTCGTGCTCCACTGGAGTACCTGAACGTGGCAG 480
15658R-1.IR_full 481 GTTGCGAGCTACAGGAGTTACC 502
||||||||||||||||||||||
15658R-1.in silico 481 GTTGCGAGCTACAGGAGTTACC 502
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_137717.2 |
CG15658-RA (CG15658), mRNA |
| 0.2 |
1 |
3 |
4 |
8 |
NM_140971.1 |
CG5195-RA (CG5195), mRNA |
| 0 |
0 |
3 |
5 |
2 |
NM_134538.1 |
CG1504-RA (CG1504), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_080329.2 |
CG3697-RA (mei-9), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_142221.1 |
CG18516-RA (CG18516), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_135917.2 |
CG18480-RA (CG18480), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_170213.2 |
CG31102-RA (CG31102), mRNA |
| 0 |
0 |
1 |
7 |
2 |
NM_168468.1 |
CG32091-RB (CG32091), mRNA |
| 0 |
0 |
1 |
6 |
14 |
NM_080358.2 |
CG1484-RA (fliI), mRNA |
| 0 |
0 |
1 |
4 |
6 |
NM_135239.1 |
CG18304-RA (CG18304), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_167532.1 |
CG9699-RD, transcript variant D (CG9699), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_167534.1 |
CG9699-RF, transcript variant F (CG9699), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_167530.1 |
CG9699-RA, transcript variant A (CG9699), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_167533.1 |
CG9699-RE, transcript variant E (CG9699), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_167531.1 |
CG9699-RB, transcript variant B (CG9699), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_132919.2 |
CG9699-RC, transcript variant C (CG9699), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_137186.2 |
CG8157-RA (CG8157), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_138108.2 |
CG3663-RA (CG3663), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_166957.1 |
CG32793-RA (CG32793), mRNA |
| 0 |
0 |
0 |
6 |
4 |
NM_136401.3 |
CG9455-RA (CG9455), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_141574.2 |
CG13318-RA (CG13318), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_169659.2 |
CG14869-RA, transcript variant A (CG14869), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_206496.1 |
CG14869-RB, transcript variant B (CG14869), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_168306.1 |
CG5651-RB, transcript variant B (CG5651), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_140015.2 |
CG5651-RA, transcript variant A (CG5651), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_001031890.1 |
CG14425-RA, transcript variant A (CG14425), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_001031891.1 |
CG18350-RO, transcript variant O (Sxl), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_001031893.1 |
CG18350-RM, transcript variant M (Sxl), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_167114.2 |
CG18350-RH, transcript variant H (Sxl), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_169829.1 |
CG7702-RB, transcript variant B (CG7702), mRNA |
|
| Wing Disc |
GFP
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Puc
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Caspase
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DAPI
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Merge
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Figure
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JNK signal
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Caspase-3 signal
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