NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 15642R-3

Symbol CG15642 Full Name CG15642

CG No CG15642 Old CG No CG15642

Synonyms CG15642

Accession No (Link to NCBI) NM_132800.1

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
[Please submit your publication] Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624 [ PubMed ID = 21666802 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCAACCTGGTGCTGCAAATGCT

Primer Seq. 3'

aatctagaggtaccCGCGTCGCTTTTGCTCATTGG

Predicted Fragment Size

IR fragment full Seq

0001 CAACCTGGTG CTGCAAATGC TGCTGCAGGC CAACACGTAT GTGTCCATGG TGTGGGCCAT 
0061 GAGCTACATG ATCCACATGC TCATACGCCT GCATCATTTG TGGAACCTGG AGGGCCTGTC 
0121 CATGCTGGTG GCCTACGTTT TGGCGGTGTC CGCTGAATCC GTACGCCTGT ACGCCGGCTA 
0181 CTCGGTGAAC CTGTGCTCTG GCGCCACCGC CATGTGGATG CTGCTCACCG TGACACCATG 
0241 CATCCTGCTG CCCGCCATGG TCTTTCTTCG CCTCTCAGCT GCCGGACGCA GTTTGTGGCT 
0301 ACGCATCATC ACGAACGCCG TGTTCGCGCT AATAGTTCTC GAGGTGATCG TGTCTCTGGT 
0361 GCACTTTGTT ATCTGCAAGC CAGGCTGTAA GCTGCCGCTG CCGCTAGAAG GGAACGAGCA 
0421 GCTGCCGGAG GAGGATGAGG AGGAGCAGCA GCAATCCGCC AGTGTGGGCA CACCCACGAC 
0481 CAATGAGCAA ATAAGCGACG CG

in silico PCR Fragment

0001 CAACCTGGTG CTGCAAATGC TGCTGCAGGC CAACACGTAT GTGTCCATGG TGTGGGCCAT 
0061 GAGCTACATG ATCCACATGC TCATACGCCT GCATCATTTG TGGAACCTGG AGGGCCTGTC 
0121 CATGCTGGTG GCCTACGTTT TGGCGGTGTC CGCTGAATCC GTACGCCTAT ACGCCGGCTA 
0181 CTCGGTGAAC CTGTGCTCTG GCGCCACCGC CATGTGGATG CTGCTCACCG TGACACCATG 
0241 CATCCTGCTG CCCGCCATGG TCTTTCTTCG CCTCTCAGCT GCCGGACGCA GTTTGTGGCT 
0301 ACGCATCATC ACGAACGCCG TGTTCGCGCT AATAGTTCTC GAGGTGATCG TGTCTCTGGT 
0361 GCACTTTGTT ATCTGCAAGC CAGGCTGTAA GCTGCCGCTG CCGCTAGAAG GGAACGAGCA 
0421 GCTGCCGGAG GAGGATGAGG AGGAGCAGCA GCAATCCGCC AGTGTGGGCA CACCCACGAC 
0481 CAATGAGCAA --AAGCGACG CG

Assemble Data


15642R-3.IR_full       1   CAACCTGGTGCTGCAAATGCTGCTGCAGGCCAACACGTATGTGTCCATGGTGTGGGCCAT 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15642R-3.in silico     1   CAACCTGGTGCTGCAAATGCTGCTGCAGGCCAACACGTATGTGTCCATGGTGTGGGCCAT 60


15642R-3.IR_full       61  GAGCTACATGATCCACATGCTCATACGCCTGCATCATTTGTGGAACCTGGAGGGCCTGTC 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15642R-3.in silico     61  GAGCTACATGATCCACATGCTCATACGCCTGCATCATTTGTGGAACCTGGAGGGCCTGTC 120


15642R-3.IR_full       121 CATGCTGGTGGCCTACGTTTTGGCGGTGTCCGCTGAATCCGTACGCCTGTACGCCGGCTA 180
                           |||||||||||||||||||||||||||||||||||||||||||||||| |||||||||||
15642R-3.in silico     121 CATGCTGGTGGCCTACGTTTTGGCGGTGTCCGCTGAATCCGTACGCCTATACGCCGGCTA 180


15642R-3.IR_full       181 CTCGGTGAACCTGTGCTCTGGCGCCACCGCCATGTGGATGCTGCTCACCGTGACACCATG 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15642R-3.in silico     181 CTCGGTGAACCTGTGCTCTGGCGCCACCGCCATGTGGATGCTGCTCACCGTGACACCATG 240


15642R-3.IR_full       241 CATCCTGCTGCCCGCCATGGTCTTTCTTCGCCTCTCAGCTGCCGGACGCAGTTTGTGGCT 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15642R-3.in silico     241 CATCCTGCTGCCCGCCATGGTCTTTCTTCGCCTCTCAGCTGCCGGACGCAGTTTGTGGCT 300


15642R-3.IR_full       301 ACGCATCATCACGAACGCCGTGTTCGCGCTAATAGTTCTCGAGGTGATCGTGTCTCTGGT 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15642R-3.in silico     301 ACGCATCATCACGAACGCCGTGTTCGCGCTAATAGTTCTCGAGGTGATCGTGTCTCTGGT 360


15642R-3.IR_full       361 GCACTTTGTTATCTGCAAGCCAGGCTGTAAGCTGCCGCTGCCGCTAGAAGGGAACGAGCA 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15642R-3.in silico     361 GCACTTTGTTATCTGCAAGCCAGGCTGTAAGCTGCCGCTGCCGCTAGAAGGGAACGAGCA 420


15642R-3.IR_full       421 GCTGCCGGAGGAGGATGAGGAGGAGCAGCAGCAATCCGCCAGTGTGGGCACACCCACGAC 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15642R-3.in silico     421 GCTGCCGGAGGAGGATGAGGAGGAGCAGCAGCAATCCGCCAGTGTGGGCACACCCACGAC 480


15642R-3.IR_full       481 CAATGAGCAAATAAGCGACGCG 502
                           ||||||||||  ||||||||||
15642R-3.in silico     481 CAATGAGCAA--AAGCGACGCG 502

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	12	NM_132800.1	CG15642-RA (CG15642), mRNA
0.2	1	6	10	17	NM_142126.1	CG3509-RA (CG3509), mRNA
0.2	1	5	3	4	NM_168352.1	CG32048-RA, transcript variant A (CG32048), mRNA
0.2	1	5	3	3	NM_168353.1	CG32048-RB, transcript variant B (CG32048), mRNA
0	0	13	44	59	NM_167523.2	CG9819-RA, transcript variant A (CanA-14F), mRNA
0	0	13	44	59	NM_167524.2	CG9819-RB, transcript variant B (CanA-14F), mRNA
0	0	12	29	37	NM_141658.3	CG8301-RA (CG8301), mRNA
0	0	9	13	26	NM_134517.2	CG15618-RA (CG15618), mRNA
0	0	8	27	62	NM_132457.1	CG11122-RA (CG11122), mRNA
0	0	6	23	49	NM_133114.2	CG32541-RA (CG32541), mRNA
0	0	6	21	77	NM_167376.1	CG7107-RD, transcript variant D (up), mRNA
0	0	6	21	77	NM_001014739.1	CG7107-RE, transcript variant E (up), mRNA
0	0	6	21	77	NM_167375.1	CG7107-RB, transcript variant B (up), mRNA
0	0	6	21	77	NM_080349.2	CG7107-RA, transcript variant A (up), mRNA
0	0	6	21	76	NM_001014737.1	CG7107-RG, transcript variant G (up), mRNA
0	0	6	21	76	NM_001014738.1	CG7107-RF, transcript variant F (up), mRNA
0	0	6	16	24	NM_167401.1	CG1517-RB, transcript variant B (na), mRNA
0	0	6	16	24	NM_167402.1	CG1517-RC, transcript variant C (na), mRNA
0	0	6	14	20	NM_134680.1	CG13691-RA (BBS8), mRNA
0	0	5	20	41	NM_170053.2	CG17894-RC, transcript variant C (cnc), mRNA
0	0	5	12	25	NM_166335.2	CG7097-RA, transcript variant A (CG7097), mRNA
0	0	5	8	19	NM_142959.2	CG6129-RB, transcript variant B (CG6129), mRNA
0	0	4	24	67	NM_132012.1	CG15776-RA (CG15776), mRNA
0	0	4	23	30	NM_135975.2	CG31781-RB (CG31781), mRNA
0	0	4	18	43	NM_133104.2	CG7282-RA (CG7282), mRNA
0	0	4	17	50	NM_078516.2	CG12690-RA (CHES-1-like), mRNA
0	0	4	17	34	NM_167309.1	CG32663-RA (CG32663), mRNA
0	0	4	16	28	NM_176374.1	CG4761-RA (knrl), mRNA
0	0	4	15	25	NM_206287.1	CG8571-RB, transcript variant B (smid), mRNA
0	0	4	15	25	NM_079235.2	CG8571-RA, transcript variant A (smid), mRNA

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