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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
15637R-1 |
| Symbol |
dp |
Full Name |
dumpy |
| CG No |
CG33196 |
Old CG No |
CG15637 |
| Synonyms |
Dp, CT35799, CG15637, SP460, DP, olv[D], l(2)24Fd, CR33195, CG31966, CG31968, CG31967, CG33000, CG31725, CG31727, CG33086, CG31965, CG31964, CG31726, CG31724, CG33196, BcDNA:SD02173, dp |
| Accession No (Link to NCBI) |
NM_175960.3 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Sakaidani Y, Nomura T, Matsuura A, Ito M, Suzuki E, Murakami K, Nadano D, Matsuda T, Furukawa K, Okajima T.
O-linked-N-acetylglucosamine on extracellular protein domains mediates epithelial cell-matrix interactions.
Nat Commun (2011) 2 583
[
PubMed ID = 22158438
]
[
RRC reference
]
Mummery-Widmer JL, Yamazaki M, Stoeger T, Novatchkova M, Bhalerao S, Chen D, Dietzl G, Dickson BJ, Knoblich JA.
Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi.
Nature (2009) 458(7241) 987-92
[
PubMed ID = 19363474
]
[
RRC reference
]
|
| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGAATGCCAAGATCCCGCGATA |
| Primer Seq. 3' |
aatctagaggtaccTCGCAGCCGTTCATGGGATCA |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 GAATGCCAAG ATCCCGCGAT AGcANCGCGA NTGCGTGGAG AACgNCGAGT GCTGCAACCT
0061 GCCGGCGCAC TTCCTCTGCA AGTGCAAGGA TGGATACGAG GGCGACGGCG AAGTTCTGTG
0121 CACGGATGTG GACGAGTGTC GCAATCCGGA GAACTGCGGT CCCAACGCTC TGTGCACCAA
0181 CACGCCGGGC AATTACACAT NGCTCCTGCC CGGACGGCTA TGTGGGCAAC AATCCGTATC
0241 GGGAGGGATG CCAGGATGTG GACGAGTGCT CGTACCCGAA TGTCTGCGGA CCCGGTGCCA
0301 TATGTACGAA TCTCGAGGGC AGTTATCGCT GCGACTGCCC GCCGGGATAC GACGGCGATG
0361 GACGCTCGGA GTCCGGTTGC GTGGATCAGG ATGAGTGCGC ACGGACgCCC TGCGGCCGGA
0421 ACGCGGNATT GTTTGAACAC GGACNGCAGC TTCCGCTGCT TGTGTCCGGA CGGCTACAGT
0481 GGTGATCCCA TGAACGGCTG CGA |
| in silico PCR Fragment |
0001 GAATGCCAAG ATCCCGCGAT AGCAGCGCGA -TGCGTGGAG AACGCCGAGT GCTGCAACCT
0061 GCCGGCGCAC TTCCTCTGCA AGTGCAAGGA TGGATACGAG GGCGACGGCG AAGTTCTGTG
0121 CACGGATGTG GACGAGTGTC GCAATCCGGA GAACTGCGGT CCCAACGCTC TGTGCACCAA
0181 CACGCCGGGC AATTACACAT -GCTCCTGCC CGGACGGCTA TGTGGGCAAC AATCCGTATC
0241 GGGAGGGATG CCAGGATGTG GACGAGTGCT CGTACCCGAA TGTCTGCGGA CCCGGTGCCA
0301 TATGTACGAA TCTCGAGGGC AGTTATCGCT GCGACTGCCC GCCGGGATAC GACGGCGATG
0361 GACGCTCGGA GTCCGGTTGC GTGGATCAGG ATGAGTGCGC ACGGACGCCC TGCGGCCGGA
0421 ACGCGG-ATT GTTTGAACAC GGACGGCAGC TTCCGCTGCT TGTGTCCGGA CGGCTACAGT
0481 GGTGATCCCA TGAACGGCTG CGA |
| Assemble Data |
15637R-1.IR_full 1 GAATGCCAAGATCCCGCGATAGCANCGCGANTGCGTGGAGAACGNCGAGTGCTGCAACCT 60
|||||||||||||||||||||||| ||||| ||||||||||||| |||||||||||||||
15637R-1.in silico 1 GAATGCCAAGATCCCGCGATAGCAGCGCGA-TGCGTGGAGAACGCCGAGTGCTGCAACCT 60
15637R-1.IR_full 61 GCCGGCGCACTTCCTCTGCAAGTGCAAGGATGGATACGAGGGCGACGGCGAAGTTCTGTG 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15637R-1.in silico 61 GCCGGCGCACTTCCTCTGCAAGTGCAAGGATGGATACGAGGGCGACGGCGAAGTTCTGTG 120
15637R-1.IR_full 121 CACGGATGTGGACGAGTGTCGCAATCCGGAGAACTGCGGTCCCAACGCTCTGTGCACCAA 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15637R-1.in silico 121 CACGGATGTGGACGAGTGTCGCAATCCGGAGAACTGCGGTCCCAACGCTCTGTGCACCAA 180
15637R-1.IR_full 181 CACGCCGGGCAATTACACATNGCTCCTGCCCGGACGGCTATGTGGGCAACAATCCGTATC 240
|||||||||||||||||||| |||||||||||||||||||||||||||||||||||||||
15637R-1.in silico 181 CACGCCGGGCAATTACACAT-GCTCCTGCCCGGACGGCTATGTGGGCAACAATCCGTATC 240
15637R-1.IR_full 241 GGGAGGGATGCCAGGATGTGGACGAGTGCTCGTACCCGAATGTCTGCGGACCCGGTGCCA 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15637R-1.in silico 241 GGGAGGGATGCCAGGATGTGGACGAGTGCTCGTACCCGAATGTCTGCGGACCCGGTGCCA 300
15637R-1.IR_full 301 TATGTACGAATCTCGAGGGCAGTTATCGCTGCGACTGCCCGCCGGGATACGACGGCGATG 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15637R-1.in silico 301 TATGTACGAATCTCGAGGGCAGTTATCGCTGCGACTGCCCGCCGGGATACGACGGCGATG 360
15637R-1.IR_full 361 GACGCTCGGAGTCCGGTTGCGTGGATCAGGATGAGTGCGCACGGACGCCCTGCGGCCGGA 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15637R-1.in silico 361 GACGCTCGGAGTCCGGTTGCGTGGATCAGGATGAGTGCGCACGGACGCCCTGCGGCCGGA 420
15637R-1.IR_full 421 ACGCGGNATTGTTTGAACACGGACNGCAGCTTCCGCTGCTTGTGTCCGGACGGCTACAGT 480
|||||| ||||||||||||||||| |||||||||||||||||||||||||||||||||||
15637R-1.in silico 421 ACGCGG-ATTGTTTGAACACGGACGGCAGCTTCCGCTGCTTGTGTCCGGACGGCTACAGT 480
15637R-1.IR_full 481 GGTGATCCCATGAACGGCTGCGA 503
|||||||||||||||||||||||
15637R-1.in silico 481 GGTGATCCCATGAACGGCTGCGA 503
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
3 |
28 |
139 |
NM_175960.3 |
CG33196-RB (dp), mRNA |
| 0 |
0 |
3 |
3 |
2 |
NM_137716.2 |
CG4286-RA (CG4286), mRNA |
| 0 |
0 |
3 |
2 |
2 |
NM_058033.3 |
CG7581-RA (Bub3), mRNA |
| 0 |
0 |
2 |
3 |
7 |
NM_139641.2 |
CG7447-RA, transcript variant A (CG7447), mRNA |
| 0 |
0 |
2 |
3 |
7 |
NM_176283.1 |
CG7447-RB, transcript variant B (CG7447), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_136616.3 |
CG8057-RA, transcript variant A (CG8057), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_206061.1 |
CG8057-RB, transcript variant B (CG8057), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_058044.4 |
CG9148-RA, transcript variant A (scf), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_206231.1 |
CG9148-RC, transcript variant C (scf), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_058045.4 |
CG9148-RB, transcript variant B (scf), mRNA |
| 0 |
0 |
1 |
4 |
10 |
NM_164582.1 |
CG3054-RA, transcript variant A (l(2)k05819), mRNA |
| 0 |
0 |
1 |
4 |
10 |
NM_134985.2 |
CG3054-RB, transcript variant B (l(2)k05819), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_167253.1 |
CG1691-RH, transcript variant H (Imp), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_167251.1 |
CG1691-RF, transcript variant F (Imp), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_167252.1 |
CG1691-RG, transcript variant G (Imp), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_167254.2 |
CG1691-RB, transcript variant B (Imp), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_001042803.1 |
CG1691-RI, transcript variant I (Imp), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_167249.1 |
CG1691-RD, transcript variant D (Imp), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_167250.1 |
CG1691-RE, transcript variant E (Imp), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_167255.1 |
CG1691-RC, transcript variant C (Imp), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_078556.3 |
CG1691-RA, transcript variant A (Imp), mRNA |
| 0 |
0 |
1 |
3 |
7 |
NM_080049.2 |
CG11614-RA (nkd), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_142241.1 |
CG4560-RB, transcript variant B (Arpc3A), mRNA |
| 0 |
0 |
1 |
2 |
8 |
NM_141055.2 |
CG6014-RA (CG6014), mRNA |
| 0 |
0 |
1 |
2 |
7 |
NM_057790.4 |
CG32848-RA (VAChT), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_139961.1 |
CG7120-RA (CG7120), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_001014718.1 |
CG33548-RB, transcript variant B (msta), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_132260.2 |
CG12113-RA (l(1)G0095), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_140044.1 |
CG4446-RA, transcript variant A (CG4446), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_206309.1 |
CG4446-RB, transcript variant B (CG4446), mRNA |
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