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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
15632R-3 |
| Symbol |
Taf12L |
Full Name |
TBP-associated factor 30kD subunit alpha-2 |
| CG No |
CG15632 |
Old CG No |
CG15632 |
| Synonyms |
dTAF12L, rye, dTAF12, TAF, TFIID, dTAF[[II]]30a, dmTAF12b, dTAF[[II]]30alpha-2, TAF[[II]], dmTAF12L, CG15632, Taf30alpha-2, TAF30alpha-2, Taf12L, TAF12L |
| Accession No (Link to NCBI) |
NM_135008.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgACATGGAACCTTGGTCCTTTG |
| Primer Seq. 3' |
aatctagaggtaccGGAACTCCATGTTGAACTCCC |
| Predicted Fragment Size |
396 |
| IR fragment full Seq |
0001 ACATGGAACC TTGGTCCTTT GCCCTCAATT TCGATGACAG CGATAAATCG TCGGATAACG
0061 AGAGCCACTC TTCGACCAGA TCCTCGAGCC GCTCCAGTGA CACCAGCATA GACACCAGCT
0121 CCGTGGAGAA GGAGCCAGCT AGCGTAATTG AGAGCCAGTC TGTTCCTGGC GGCAGCTACG
0181 ATATTATCTC CAAGACCAAC ATGCTGCAAT TTGTGCAGAA AATCGATGCA AATTCATCGC
0241 TGGACGATCA AGGTTGCGAT ATGATGGCCA GAATNAGCCG ATGCCTTTGT TAACGACATA
0301 TCCATGCGCA TAGTTAAGTT GGCCAAGTAT CGAAAGAGCG ACGTTAGCGT GCTGGACCTG
0361 AAGTTCATCC TCAAGCGGGA GTTCAACATG GATGTTCC |
| in silico PCR Fragment |
0001 ACATGGAACC TTGGTCCTTT GCCCTCAATT TCGATGACAG CGATAAATCG TCGGATAACG
0061 AGAGCCACTC TTCGACCAGA TCCTCGAGCC GCTCCAGTGA CACCAGCATA GACACCAGCT
0121 CCGTGGAGAA GGAGCCAGCT AGCGTAATTG AGAGCCAGTC TGTCCCTGGC GGAAGCTACG
0181 ATATTATCTC CAAGACCAAC ATGCTGCAAT TTGTGCAGAA AATCGATGCA AATTCATCGC
0241 TGGACGATCA AGGTTGCGAT ATGATGGCCA GAAT-AGCCG ATGCCTTTGT TAACGACATA
0301 TCCATGCGCA TAGTTAAGTT GGCCAAGTAT CGAAAGAGCG ACGTTAGCGT GCTGGACCTG
0361 AAGTTCATCC TCAAGCGGGA GTTCAACATG GA-GTTCC |
| Assemble Data |
15632R-3.IR_full 1 ACATGGAACCTTGGTCCTTTGCCCTCAATTTCGATGACAGCGATAAATCGTCGGATAACG 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15632R-3.in silico 1 ACATGGAACCTTGGTCCTTTGCCCTCAATTTCGATGACAGCGATAAATCGTCGGATAACG 60
15632R-3.IR_full 61 AGAGCCACTCTTCGACCAGATCCTCGAGCCGCTCCAGTGACACCAGCATAGACACCAGCT 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15632R-3.in silico 61 AGAGCCACTCTTCGACCAGATCCTCGAGCCGCTCCAGTGACACCAGCATAGACACCAGCT 120
15632R-3.IR_full 121 CCGTGGAGAAGGAGCCAGCTAGCGTAATTGAGAGCCAGTCTGTTCCTGGCGGCAGCTACG 180
||||||||||||||||||||||||||||||||||||||||||| |||||||| |||||||
15632R-3.in silico 121 CCGTGGAGAAGGAGCCAGCTAGCGTAATTGAGAGCCAGTCTGTCCCTGGCGGAAGCTACG 180
15632R-3.IR_full 181 ATATTATCTCCAAGACCAACATGCTGCAATTTGTGCAGAAAATCGATGCAAATTCATCGC 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15632R-3.in silico 181 ATATTATCTCCAAGACCAACATGCTGCAATTTGTGCAGAAAATCGATGCAAATTCATCGC 240
15632R-3.IR_full 241 TGGACGATCAAGGTTGCGATATGATGGCCAGAATNAGCCGATGCCTTTGTTAACGACATA 300
|||||||||||||||||||||||||||||||||| |||||||||||||||||||||||||
15632R-3.in silico 241 TGGACGATCAAGGTTGCGATATGATGGCCAGAAT-AGCCGATGCCTTTGTTAACGACATA 300
15632R-3.IR_full 301 TCCATGCGCATAGTTAAGTTGGCCAAGTATCGAAAGAGCGACGTTAGCGTGCTGGACCTG 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15632R-3.in silico 301 TCCATGCGCATAGTTAAGTTGGCCAAGTATCGAAAGAGCGACGTTAGCGTGCTGGACCTG 360
15632R-3.IR_full 361 AAGTTCATCCTCAAGCGGGAGTTCAACATGGATGTTCC 398
|||||||||||||||||||||||||||||||| |||||
15632R-3.in silico 361 AAGTTCATCCTCAAGCGGGAGTTCAACATGGA-GTTCC 398
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
378 |
0 |
0 |
0 |
NM_135008.2 |
CG15632-RA (Taf12L), mRNA |
| 0 |
0 |
3 |
2 |
2 |
NM_137460.2 |
CG5726-RA (CG5726), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_169451.1 |
CG31361-RA, transcript variant A (dpr17), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_165423.1 |
CG3136-RA, transcript variant A (CG3136), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_136315.2 |
CG3136-RB, transcript variant B (CG3136), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_132434.2 |
CG1637-RC, transcript variant C (CG1637), mRNA |
| 0 |
0 |
1 |
3 |
9 |
NM_001032053.1 |
CG33715-RB, transcript variant B (Msp-300), mRNA |
| 0 |
0 |
1 |
3 |
9 |
NM_001032051.1 |
CG33715-RD, transcript variant D (Msp-300), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_132610.2 |
CG4404-RA (CG4404), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_132831.1 |
CG8184-RB (CG8184), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_078976.3 |
CG9015-RA, transcript variant A (en), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_165841.1 |
CG9015-RB, transcript variant B (en), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_057261.2 |
CG3821-RA (Aats-asp), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_167325.1 |
CG11138-RB, transcript variant B (CG11138), mRNA |
| 0 |
0 |
0 |
7 |
5 |
NM_079770.2 |
CG7748-RA (OstStt3), mRNA |
| 0 |
0 |
0 |
6 |
18 |
NM_206687.1 |
CG33235-RA (CG33235), mRNA |
| 0 |
0 |
0 |
6 |
5 |
NM_175945.1 |
CG4297-RB, transcript variant B (CG4297), mRNA |
| 0 |
0 |
0 |
6 |
5 |
NM_134683.1 |
CG4297-RA, transcript variant A (CG4297), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_167375.1 |
CG7107-RB, transcript variant B (up), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_080349.2 |
CG7107-RA, transcript variant A (up), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_001014737.1 |
CG7107-RG, transcript variant G (up), mRNA |
| 0 |
0 |
0 |
5 |
2 |
NM_001043190.1 |
CG14636-RB, transcript variant B (CG14636), mRNA |
| 0 |
0 |
0 |
5 |
2 |
NM_141180.2 |
CG14636-RA, transcript variant A (CG14636), mRNA |
| 0 |
0 |
0 |
4 |
15 |
NM_001038866.1 |
CG33981-RB, transcript variant B (CG33981), mRNA |
| 0 |
0 |
0 |
4 |
15 |
NM_001038865.1 |
CG33981-RA, transcript variant A (CG33981), mRNA |
| 0 |
0 |
0 |
4 |
15 |
NM_206158.1 |
CG6355-RB, transcript variant B (CG6355), mRNA |
| 0 |
0 |
0 |
4 |
15 |
NM_137425.3 |
CG6355-RA, transcript variant A (CG6355), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_145191.1 |
CG4098-RA (CG4098), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_140650.2 |
CG18217-RA (CG18217), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_206023.1 |
CG3136-RC, transcript variant C (CG3136), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
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Figure
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JNK signal
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Caspase-3 signal
| N |
 |
| + |
|
| DB |
 |
| ++ |
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| DC |
 |
| ++ |
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| DM |
 |
| ++ |
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| VM |
 |
| + |
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| VB |
 |
| |
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| Dcompartment |
|
Penetrance |
| + |
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