NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 15627R-1

Symbol CG15627 Full Name CG15627

CG No CG15627 Old CG No CG15627

Synonyms CT35778, CG15627

Accession No (Link to NCBI) NM_135019.1

Inserted Chr. ll

Insertional Mutation lethal

Phenotype induced by Act5C-GAL4 at 28 degrees viable

Map Viewer

Reference
[Please submit your publication] Chen C, Buhl E, Xu M, Croset V, Rees JS, Lilley KS, Benton R, Hodge JJ, Stanewsky R.
Drosophila Ionotropic Receptor 25a mediates circadian clock resetting by temperature.
Nature (2015) 527(7579) 516-20 [ PubMed ID = 26580016 ] [ RRC reference ]

Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCCATCCCTTTGGATAGTGTTC

Primer Seq. 3'

aatctagaggtaccTGCAGCAGGGACTTGTACTTG

Predicted Fragment Size

IR fragment full Seq

0001 CCATCCCTTT GGATAGTGTT CATCAACGAG GTGGACAATG AGCCGGCTGC GAAGGcTGTG 
0061 GAGGTGGTGC TCACCTACTT GAAGAAGAAC ATACGATATG GTCTATCGGT GCAACTAGAT 
0121 TCGATAGAGG CGAACAAGTC CGATGCCAAG GTGCTGCTGG AAGCTAGTGA GTTGTGTTTG 
0181 CAATAAGTAC GCAACAAGTA TTGAGAAGAA ACAAACGCCC CATCTGATCC TGGACACCAC 
0241 CAAATCGGGC ATAGCCTCGG AAACGGTGAA GAGCTTCANC CCAGGCTCTG GGTCTGCCCA 
0301 CCATTAGTGC CTCCTATGGC CAGCAGGGCG ACTTGAGGCA GTGGCGCGAC TTGGATGAGG 
0361 NGAAGCAGAA GTATTTGCTG CAGGTGAtNC CGCCGGCGGA TATTATTCCC GAGGCCATTC 
0421 GAAGTATAGT GATTCACATG AACATCACGA ATGCTGCCAT TCTGTACGAT GATTCCTTTG 
0481 TCATGGACCA CAAGTACAAG TCCCTGCTGC A

in silico PCR Fragment

0001 CCATCCCTTT GGATAGTGTT CATCAACGAG GTGGACAATG AGCCGGCTGC GAAGGCTGTG 
0061 GAGGTGGTGC TCACCTACTT GAAGAAGAAC ATACGATATG GTCTATCGGT GCAACTAGAT 
0121 TCGATAGAGG CGAACAAGTC CGATGCCAAG GTGCTGCTGG AAGCTA---- ------TTTG 
0181 CAATAAGTAC GCAACAAGTA TTGAGAAGAA ACAAACGCCC CATCTGATCC TGGACACCAC 
0241 CAAATCGGGC ATAGCCTCGG AAACGGTGAA GAGCTTCA-C CCAGGCTCTG GGTCTGCCCA 
0301 CCATTAGTGC CTCCTATGGC CAGCAGGGCG ACTTGAGGCA GTGGCGCGAC TTGGATGAGG 
0361 CGAAGCAGAA GTATTTGCTG CAGGTGATGC CGCCGGCGGA TATTATTCCC GAGGCCATTC 
0421 GAAGTATAGT GATTCACATG AACATCACGA ATGCTGCCAT TCTGTACGAT GATTCCTTTG 
0481 TCATGGACCA CAAGTACAAG TCCCTGCTGC A

Assemble Data


15627R-1.IR_full       1   CCATCCCTTTGGATAGTGTTCATCAACGAGGTGGACAATGAGCCGGCTGCGAAGGCTGTG 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15627R-1.in silico     1   CCATCCCTTTGGATAGTGTTCATCAACGAGGTGGACAATGAGCCGGCTGCGAAGGCTGTG 60


15627R-1.IR_full       61  GAGGTGGTGCTCACCTACTTGAAGAAGAACATACGATATGGTCTATCGGTGCAACTAGAT 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15627R-1.in silico     61  GAGGTGGTGCTCACCTACTTGAAGAAGAACATACGATATGGTCTATCGGTGCAACTAGAT 120


15627R-1.IR_full       121 TCGATAGAGGCGAACAAGTCCGATGCCAAGGTGCTGCTGGAAGCTAGTGAGTTGTGTTTG 180
                           ||||||||||||||||||||||||||||||||||||||||||||||          ||||
15627R-1.in silico     121 TCGATAGAGGCGAACAAGTCCGATGCCAAGGTGCTGCTGGAAGCTA----------TTTG 180


15627R-1.IR_full       181 CAATAAGTACGCAACAAGTATTGAGAAGAAACAAACGCCCCATCTGATCCTGGACACCAC 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15627R-1.in silico     181 CAATAAGTACGCAACAAGTATTGAGAAGAAACAAACGCCCCATCTGATCCTGGACACCAC 240


15627R-1.IR_full       241 CAAATCGGGCATAGCCTCGGAAACGGTGAAGAGCTTCANCCCAGGCTCTGGGTCTGCCCA 300
                           |||||||||||||||||||||||||||||||||||||| |||||||||||||||||||||
15627R-1.in silico     241 CAAATCGGGCATAGCCTCGGAAACGGTGAAGAGCTTCA-CCCAGGCTCTGGGTCTGCCCA 300


15627R-1.IR_full       301 CCATTAGTGCCTCCTATGGCCAGCAGGGCGACTTGAGGCAGTGGCGCGACTTGGATGAGG 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15627R-1.in silico     301 CCATTAGTGCCTCCTATGGCCAGCAGGGCGACTTGAGGCAGTGGCGCGACTTGGATGAGG 360


15627R-1.IR_full        361 GAAGCAGAAGTATTTGCTGCAGGTGATNCCGCCGGCGGATATTATTCCCGAGGCCATTC 419
                            ||||||||||||||||||||||||||| |||||||||||||||||||||||||||||||
15627R-1.in silico      361 GAAGCAGAAGTATTTGCTGCAGGTGATGCCGCCGGCGGATATTATTCCCGAGGCCATTC 419


15627R-1.IR_full       421 GAAGTATAGTGATTCACATGAACATCACGAATGCTGCCATTCTGTACGATGATTCCTTTG 479
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15627R-1.in silico     421 GAAGTATAGTGATTCACATGAACATCACGAATGCTGCCATTCTGTACGATGATTCCTTTG 479


15627R-1.IR_full       481 TCATGGACCACAAGTACAAGTCCCTGCTGCA 510
                           |||||||||||||||||||||||||||||||
15627R-1.in silico     481 TCATGGACCACAAGTACAAGTCCCTGCTGCA 510

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_135019.1	CG15627-RA (CG15627), mRNA
0.2	1	3	2	6	NM_137629.1	CG13871-RA (CG13871), mRNA
0	0	3	3	2	NM_080013.2	CG6050-RA (EfTuM), mRNA
0	0	3	2	5	NM_141075.1	CG7634-RA (CG7634), mRNA
0	0	2	3	4	NM_140231.1	CG6071-RA (CG6071), mRNA
0	0	2	2	2	NM_143785.2	CG9762-RA (l(3)neo18), mRNA
0	0	1	5	7	NM_166604.1	CG9812-RC, transcript variant C (CG9812), mRNA
0	0	1	5	7	NM_137949.2	CG9812-RB, transcript variant B (CG9812), mRNA
0	0	1	4	7	NM_057343.3	CG9327-RA (Pros29), mRNA
0	0	1	4	2	NM_080138.2	CG1618-RA (comt), mRNA
0	0	1	4	2	NM_079211.2	CG10583-RA (Sse), mRNA
0	0	1	3	5	NM_132794.2	CG18210-RA (CG18210), mRNA
0	0	1	3	2	NM_169345.1	CG31395-RA (CG31395), mRNA
0	0	1	3	2	NM_132991.1	CG12432-RA (CG12432), mRNA
0	0	1	2	6	NM_057738.3	CG3969-RA, transcript variant A (PR2), mRNA
0	0	1	2	6	NM_057739.3	CG3969-RB, transcript variant B (PR2), mRNA
0	0	1	2	5	NM_137162.1	CG10253-RA (CG10253), mRNA
0	0	1	2	4	NM_140876.1	CG8798-RA, transcript variant A (CG8798), mRNA
0	0	1	2	4	NM_168806.1	CG8798-RB, transcript variant B (CG8798), mRNA
0	0	1	2	3	NM_136925.1	CG30042-RA (CG30042), mRNA
0	0	1	2	3	NM_143530.2	CG2217-RA (CG2217), mRNA
0	0	1	2	3	NM_170493.1	CG31020-RA (spdo), mRNA
0	0	1	2	2	NM_141337.1	CG2031-RA (Hpr1), mRNA
0	0	1	2	2	NM_167461.1	CG8962-RA, transcript variant A (Paf-AHalpha), mRNA
0	0	1	2	2	NM_080350.3	CG8962-RB, transcript variant B (Paf-AHalpha), mRNA
0	0	1	2	2	NM_057938.3	CG3093-RA (dor), mRNA
0	0	0	6	3	NM_132811.2	CG7860-RA (CG7860), mRNA
0	0	0	5	18	NM_078670.2	CG7092-RA (Dhc16F), mRNA
0	0	0	5	3	NM_143524.2	CG9743-RA (CG9743), mRNA
0	0	0	4	10	NM_132004.2	CG4136-RA (CG4136), mRNA

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