NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 15596R-3

Symbol Osi11 Full Name Osiris 11

CG No CG15596 Old CG No CG15596

Synonyms osi11, CG15596, Osi11

Accession No (Link to NCBI) NM_141373.1

Inserted Chr. X

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees viable

Map Viewer

Reference
[Please submit your publication] Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgATGGAAGCACCGTCGAACTAT

Primer Seq. 3'

aatctagaggtaccAACAGAACGGCGGCAATGAAG

Predicted Fragment Size

IR fragment full Seq

0001 ATGGAAGCAC CGTCGAACTA TANTCAGAAC TCTACTGAGA GTGGACTGCT GCGGACAGTG 
0061 CGTCATATCT ATGGGCAGTG TGCATATAGC GAGGACGTTT TCTGGTGCTG CAAGATACAG 
0121 GGAGTTCGCC TGCTGGGACG GGCTCTGAAG GTCCCTCAAC TGGGGATTGT GGATGGCGTT 
0181 AGCTTGGTGC GGCGGGAATC CNTTTACCCA GGACACGCGG AGTGGACGCT CTAGCCTGCT 
0241 CGAGAGCCAG CTCAGCAATC GCGATCTCGA GCACTTGTCG GGGAAGAGTC TGGACGCCCT 
0301 GCTGCTGGAG CGGTTCCTCA ACTTTGTCCA TAGTCACCAA CTGCAGGTCA ACCTACCACG 
0361 GTTGCTGCGG TTTGGTGAGC GGAATGTCCA GGATTGGCTG CTGCACGTGG TCGGCTACTT 
0421 TATGCCAGCC AGCGAAAGCG AGGGTCGCAA AAAGAAGGAT GACAAAAAGT ATCTGGGACC 
0481 CTTCATTGcC GCCGTTCTNN N

in silico PCR Fragment

0001 ATGGAAGCAC CGTCGAACTA TAATCAGAAC TCTACTGAGA GTGGACTGCT GCGGACAGTG 
0061 CGTCATATCT ATGGGCAGTG TGCATATAGC GAGGACGTTT TCTGGTGCTG CAAGATACAG 
0121 GGAGTTCGCC TGCTGGGACG GGCTCTGAAG GTCCCTCAAC TGGGGATTGT GGATGGCGTT 
0181 AGCTTGGTGC GGCGGGAATC C-TTTACCCA GGACACGCGG AGTGGACGCT CTAGCCTGCT 
0241 CGAGAGCCAG CTCAGCAATC GCGATCTCGA GCACTTGTCG GGGAAGAGTC TGGACGCCCT 
0301 GCTGCTGGAG CGGTTCCTCA ACTTTGTCCA TAGTCACCAG CTGCAGGTCA ACCTACCACG 
0361 GTTGCTGCGG TTTGGTGAGC GGAATGTCCA GGATTGGCTG CTGCACGTGG TCGGCTACTT 
0421 TATGCCAGCC AGCGAAAGCG AGGGTCGCAA AAAGAAGGAT GACAAAAAGT ATCTGGGACC 
0481 CTTCATTGCC GCCGTTCTGT T

Assemble Data


15596R-3.IR_full       1   ATGGAAGCACCGTCGAACTATANTCAGAACTCTACTGAGAGTGGACTGCTGCGGACAGTG 60
                           |||||||||||||||||||||| |||||||||||||||||||||||||||||||||||||
15596R-3.in silico     1   ATGGAAGCACCGTCGAACTATAATCAGAACTCTACTGAGAGTGGACTGCTGCGGACAGTG 60


15596R-3.IR_full       61  CGTCATATCTATGGGCAGTGTGCATATAGCGAGGACGTTTTCTGGTGCTGCAAGATACAG 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15596R-3.in silico     61  CGTCATATCTATGGGCAGTGTGCATATAGCGAGGACGTTTTCTGGTGCTGCAAGATACAG 120


15596R-3.IR_full       121 GGAGTTCGCCTGCTGGGACGGGCTCTGAAGGTCCCTCAACTGGGGATTGTGGATGGCGTT 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15596R-3.in silico     121 GGAGTTCGCCTGCTGGGACGGGCTCTGAAGGTCCCTCAACTGGGGATTGTGGATGGCGTT 180


15596R-3.IR_full       181 AGCTTGGTGCGGCGGGAATCCNTTTACCCAGGACACGCGGAGTGGACGCTCTAGCCTGCT 240
                           ||||||||||||||||||||| ||||||||||||||||||||||||||||||||||||||
15596R-3.in silico     181 AGCTTGGTGCGGCGGGAATCC-TTTACCCAGGACACGCGGAGTGGACGCTCTAGCCTGCT 240


15596R-3.IR_full       241 CGAGAGCCAGCTCAGCAATCGCGATCTCGAGCACTTGTCGGGGAAGAGTCTGGACGCCCT 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15596R-3.in silico     241 CGAGAGCCAGCTCAGCAATCGCGATCTCGAGCACTTGTCGGGGAAGAGTCTGGACGCCCT 300


15596R-3.IR_full       301 GCTGCTGGAGCGGTTCCTCAACTTTGTCCATAGTCACCAACTGCAGGTCAACCTACCACG 360
                           ||||||||||||||||||||||||||||||||||||||| ||||||||||||||||||||
15596R-3.in silico     301 GCTGCTGGAGCGGTTCCTCAACTTTGTCCATAGTCACCAGCTGCAGGTCAACCTACCACG 360


15596R-3.IR_full       361 GTTGCTGCGGTTTGGTGAGCGGAATGTCCAGGATTGGCTGCTGCACGTGGTCGGCTACTT 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15596R-3.in silico     361 GTTGCTGCGGTTTGGTGAGCGGAATGTCCAGGATTGGCTGCTGCACGTGGTCGGCTACTT 420


15596R-3.IR_full       421 TATGCCAGCCAGCGAAAGCGAGGGTCGCAAAAAGAAGGATGACAAAAAGTATCTGGGACC 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15596R-3.in silico     421 TATGCCAGCCAGCGAAAGCGAGGGTCGCAAAAAGAAGGATGACAAAAAGTATCTGGGACC 480


15596R-3.IR_full       481 CTTCATTGCCGCCGTTCTNNN 501
                           ||||||||||||||||||   
15596R-3.in silico     481 CTTCATTGCCGCCGTTCTGTT 501

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	1	NM_141373.1	CG15596-RA (Osi11), mRNA
0	0	4	2	4	NM_167285.2	CG11727-RA, transcript variant A (CG11727), mRNA
0	0	4	2	2	NM_132488.3	CG11727-RB, transcript variant B (CG11727), mRNA
0	0	3	3	5	NM_206653.1	CG32717-RE, transcript variant E (sdt), mRNA
0	0	3	3	5	NM_132235.2	CG32717-RB, transcript variant B (sdt), mRNA
0	0	3	3	4	NM_001042798.1	CG32717-RH, transcript variant H (sdt), mRNA
0	0	3	3	4	NM_001038747.1	CG32717-RF, transcript variant F (sdt), mRNA
0	0	3	3	3	NM_206652.1	CG32717-RD, transcript variant D (sdt), mRNA
0	0	1	6	2	NM_137537.2	CG15099-RA (CG15099), mRNA
0	0	1	5	1	NM_132874.1	CG14948-RA (dpr18), mRNA
0	0	1	4	2	NM_169131.1	CG15593-RA, transcript variant A (Osi10), mRNA
0	0	1	4	2	NM_169362.1	CG6544-RB, transcript variant B (fau), mRNA
0	0	1	3	3	NM_080712.5	CG1693-RA, transcript variant A (tty), mRNA
0	0	1	3	3	NM_138010.3	CG16783-RA (fzr2), mRNA
0	0	1	3	3	NM_142503.2	CG14291-RA (CG14291), mRNA
0	0	1	3	2	NM_167744.3	CG1693-RB, transcript variant B (tty), mRNA
0	0	1	3	2	NM_057401.3	CG3068-RA (aur), mRNA
0	0	1	2	5	NM_078559.2	CG18085-RA (sev), mRNA
0	0	1	2	3	NM_080310.2	CG7952-RB (gt), mRNA
0	0	1	2	3	NM_135608.1	CG6724-RA (CG6724), mRNA
0	0	1	2	2	NM_170650.3	CG31045-RC, transcript variant C (Mhcl), mRNA
0	0	0	9	4	NM_079408.4	CG5492-RB, transcript variant B (Tsp74F), mRNA
0	0	0	9	4	NM_168754.1	CG5492-RA, transcript variant A (Tsp74F), mRNA
0	0	0	5	3	NM_170272.1	CG32849-RA (Hex-t2), mRNA
0	0	0	5	2	NM_140995.2	CG3947-RA (CG3947), mRNA
0	0	0	5	2	NM_132198.2	CG1530-RA (CG1530), mRNA
0	0	0	4	9	NM_169177.1	CG2507-RB, transcript variant B (sas), mRNA
0	0	0	4	5	NM_079508.2	CG2534-RA, transcript variant A (cno), mRNA
0	0	0	4	5	NM_169025.1	CG2534-RB, transcript variant B (cno), mRNA
0	0	0	4	5	NM_139443.1	CG13809-RA (osm-1), mRNA

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