NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 15592R-1

Symbol Osi9 Full Name Osiris 9

CG No CG15592 Old CG No CG15592

Synonyms CG15592, Osi9

Accession No (Link to NCBI) NM_141371.2

Inserted Chr. ll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees semi-lethal

Map Viewer

Reference
[Please submit your publication] Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCTTCTTCGGCACCCACACGCT

Primer Seq. 3'

aatctagaggtaccCTTATACGCCGCATAGGCCAG

Predicted Fragment Size

IR fragment full Seq

0001 NTTCTTCGGC ACCCACACGC TGCAGTTCAA GGTGCCCAAA GACTCCATCC AGGACATGCA 
0061 GCgNGCCCTC GAGGAATCTC GCGGCAAGAA GAAGGAGAAG AAGAAGTACC TGATGCCCCT 
0121 GCTAATGCTC TTCAAGCTGA AGATGGCCGC CCTGCTGCCC CTGGNCATTG GCTTCCTGGC 
0181 CCTGATCTCC TTCAAGGCCC TGGTCATCGG CAAGANTCGC CCTGCTGCTC TCCGGCATCA 
0241 TTGGGCTGAA GAAACTGCTC GAGTCGAAGA AGGAGAACTA CGAGGTCGTT GCGCACCCGC 
0301 ANCTACGAAC ACGAGCACAG CTACGGCCGC TCGCTGCCAT CTGATGACTC CCAGgccCAG 
0361 CAGCTgGCNT ATGCGGCGTA TAAG

in silico PCR Fragment

0001 CTTCTTCGGC ACCCACACGC TGCAGTTCAA GGTGCCCAAA GACTCCATCC AGGACATGCA 
0061 GCGCGCCCTC GAGGAATCTC GCGGCAAGAA GAAGGAGAAG AAGAAGTACC TGATGCCCCT 
0121 GCTAATGCTC TTCAAGCTGA AGATGGCCGC CCTGCTGCCC CTGGCCATTG GCTTCCTGGC 
0181 CCTGATCTCC TTCAAGGCCC TGGTCATCGG CAAGA-TCGC CCTGCTGCTC TCCGGCATCA 
0241 TTGGGCTGAA GAAACTGCTC GAGTCGAAGA AGGAGAACTA CGAGGTCGTT GCGCACCCGC 
0301 A-CTACGAGC ACGAGCACAG CTACGGCCGC TCGCTGCCAT CTGATGACTC CCAGGCCCAG 
0361 CAGCTGGCCT ATGCGGCGTA TAAG

Assemble Data


15592R-1.IR_full        1   TTCTTCGGCACCCACACGCTGCAGTTCAAGGTGCCCAAAGACTCCATCCAGGACATGCA 59
                            |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15592R-1.in silico      1   TTCTTCGGCACCCACACGCTGCAGTTCAAGGTGCCCAAAGACTCCATCCAGGACATGCA 59


15592R-1.IR_full       61  GCGNGCCCTCGAGGAATCTCGCGGCAAGAAGAAGGAGAAGAAGAAGTACCTGATGCCCCT 119
                           ||| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15592R-1.in silico     61  GCGCGCCCTCGAGGAATCTCGCGGCAAGAAGAAGGAGAAGAAGAAGTACCTGATGCCCCT 119


15592R-1.IR_full       121 GCTAATGCTCTTCAAGCTGAAGATGGCCGCCCTGCTGCCCCTGGNCATTGGCTTCCTGGC 179
                           |||||||||||||||||||||||||||||||||||||||||||| |||||||||||||||
15592R-1.in silico     121 GCTAATGCTCTTCAAGCTGAAGATGGCCGCCCTGCTGCCCCTGGCCATTGGCTTCCTGGC 179


15592R-1.IR_full       181 CCTGATCTCCTTCAAGGCCCTGGTCATCGGCAAGANTCGCCCTGCTGCTCTCCGGCATCA 239
                           ||||||||||||||||||||||||||||||||||| ||||||||||||||||||||||||
15592R-1.in silico     181 CCTGATCTCCTTCAAGGCCCTGGTCATCGGCAAGA-TCGCCCTGCTGCTCTCCGGCATCA 239


15592R-1.IR_full       241 TTGGGCTGAAGAAACTGCTCGAGTCGAAGAAGGAGAACTACGAGGTCGTTGCGCACCCGC 299
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15592R-1.in silico     241 TTGGGCTGAAGAAACTGCTCGAGTCGAAGAAGGAGAACTACGAGGTCGTTGCGCACCCGC 299


15592R-1.IR_full       301 ANCTACGAACACGAGCACAGCTACGGCCGCTCGCTGCCATCTGATGACTCCCAGGCCCAG 359
                           | |||||| |||||||||||||||||||||||||||||||||||||||||||||||||||
15592R-1.in silico     301 A-CTACGAGCACGAGCACAGCTACGGCCGCTCGCTGCCATCTGATGACTCCCAGGCCCAG 359


15592R-1.IR_full       361 CAGCTGGCNTATGCGGCGTATAAG 383
                           |||||||| |||||||||||||||
15592R-1.in silico     361 CAGCTGGCCTATGCGGCGTATAAG 383

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	364	0	0	7	NM_141371.2	CG15592-RA (Osi9), mRNA
0.82	3	3	3	22	NM_168387.1	CG6711-RA (Taf2), mRNA
0.82	3	2	3	15	NM_166347.1	CG9325-RB, transcript variant B (hts), mRNA
0.82	3	2	3	15	NM_166349.1	CG9325-RF, transcript variant F (hts), mRNA
0.82	3	2	3	15	NM_166348.1	CG9325-RE, transcript variant E (hts), mRNA
0.27	1	3	3	4	NM_079702.2	CG3637-RA (Cortactin), mRNA
0.27	1	2	3	5	NM_142429.2	CG7957-RA (MED17), mRNA
0	0	9	10	15	NM_139541.1	CG12017-RA (CG12017), mRNA
0	0	4	6	8	NM_057282.3	CG4087-RA (RpLP1), mRNA
0	0	4	4	10	NM_078691.2	CG12532-RA (Bap), mRNA
0	0	3	12	32	NM_140709.1	CG13724-RA (CG13724), mRNA
0	0	3	10	11	NM_141374.1	CG1154-RA (Osi12), mRNA
0	0	3	5	4	NM_142392.2	CG12334-RA (Atg8b), mRNA
0	0	3	5	2	NM_167245.1	CG32672-RA (Atg8a), mRNA
0	0	3	4	15	NM_140915.3	CG14183-RA (CG14183), mRNA
0	0	3	4	11	NM_132790.1	CG9123-RA (CG9123), mRNA
0	0	3	3	5	NM_139457.1	CG13801-RA (CG13801), mRNA
0	0	3	3	4	NM_168892.1	CG18023-RB, transcript variant B (Eip78C), mRNA
0	0	3	3	4	NM_079471.2	CG18023-RA, transcript variant A (Eip78C), mRNA
0	0	3	3	4	NM_169340.1	CG12592-RA (CG12592), mRNA
0	0	3	2	2	NM_079826.2	CG7610-RA, transcript variant A (ATPsyn-gamma), mRNA
0	0	3	2	2	NM_170425.2	CG7610-RB, transcript variant B (ATPsyn-gamma), mRNA
0	0	3	2	2	NM_170426.1	CG7610-RC, transcript variant C (ATPsyn-gamma), mRNA
0	0	2	7	21	NM_166126.1	CG18255-RC, transcript variant C (Strn-Mlck), mRNA
0	0	2	7	16	NM_166130.1	CG18255-RE, transcript variant E (Strn-Mlck), mRNA
0	0	2	7	15	NM_142665.1	CG15697-RA, transcript variant A (RpS30), mRNA
0	0	2	7	15	NM_169933.1	CG15697-RB, transcript variant B (RpS30), mRNA
0	0	2	5	10	NM_140412.1	CG8833-RA (CG8833), mRNA
0	0	2	3	7	NM_140665.1	CG9674-RA, transcript variant A (CG9674), mRNA
0	0	2	3	7	NM_176339.1	CG9674-RD, transcript variant D (CG9674), mRNA

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