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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 15553R-1 
 Symbol CG15553  Full Name CG15553 
 CG No CG15553  Old CG No CG15553 
 Synonyms CG15553 
 Accession No (Link to NCBI) NM_143584.2 
 Inserted Chr. ll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgGGGCGCCTGTTCAACATGTTC 
 Primer Seq. 3' 
aatctagaggtaccTTCATGTCCGTTGTGTCCGTG 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 GGGCGCCTGT TCAACATGTT CTACATAGAA CCGGTGCTTT TTATGCTGAT ATTTTCGCAT 
0061 ATGCTGTCAG GTACTGTCAT GCGCAATCAG TTGATTTATC AGGCCTGCAC GGTGATATTC 
0121 CAGTACAATG AAACCGATTG TAAGCTATTG GATAGCAAAA ACACCACCTC AGAAATACAG 
0181 GCCATTGAGA CGGAGCTTCA GGATTACGTG GCTAACATGT TTCTAACCCG CACCCTCTTC 
0241 GAAAGTATTA TGCCCGCCAT CTGTGGCTTA TTCGTTGGAT CCTGGTCGGA TCAGTATGGA 
0301 CGCAAGCCCC TGATGATTGT CTCCCTGGTC GGCTTTTCCG CTTCTGCCCT AATCTCGTCT 
0361 ATCATTTGCT GGCTGTCCAG CTACTATATG GTTAATCCCT GGTGGTACAC CTTGGCAGCC 
0421 GTGCCACACT CTGTGCTCGG CGGATGGTGT GTATTTTCTG TGGCTGCATT CTGCTTTATC 
0481 ACGGACACAA CGGAcATGAA  
 in silico PCR Fragment 
0001 GGGCGCCTGT TCAACATGTT CTACATAGAA CCGGTGCTTT TTATGCTGAT ATTTTCGCAT 
0061 ATGCTGTCAG GTACTGTCAT GCGCAATCAG TTGATTTATC AGGCCTGCAC GGTGATATTC 
0121 CAGTACAATG AAACCGATTG TAAGCTATTG GATAGCAAAA ACACCACCAC AGAAATACAG 
0181 GCAATTGAGA CGGAGCTTCA GGATTACGTG GCTAACATGT TTTTAACCCG CACCCTCTTC 
0241 GAAAGTATTA TGCCTGCCAT CTGTGGCTTA TTCGTTGGAT CCTGGTCGGA TCAGTATGGA 
0301 CGCAAGCCCC TGATGATTGT CTCCCTGGTC GGCTTTTCCG CTTCTGCCCT AATCTCGTCT 
0361 ATCATTTGCT GGCTGTCCAG CTACTATATG GTTAATCCCT GGTGGTACAC CTTGGCAGCC 
0421 GTGCCACACT CTGTGCTCGG CGGATGGTGT GTATTTTCTG TGGCTGCATT CTGCTTTATC 
0481 ACGGACACAA CGGACATGAA  
 Assemble Data 

15553R-1.IR_full       1   GGGCGCCTGTTCAACATGTTCTACATAGAACCGGTGCTTTTTATGCTGATATTTTCGCAT 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15553R-1.in silico     1   GGGCGCCTGTTCAACATGTTCTACATAGAACCGGTGCTTTTTATGCTGATATTTTCGCAT 60


15553R-1.IR_full       61  ATGCTGTCAGGTACTGTCATGCGCAATCAGTTGATTTATCAGGCCTGCACGGTGATATTC 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15553R-1.in silico     61  ATGCTGTCAGGTACTGTCATGCGCAATCAGTTGATTTATCAGGCCTGCACGGTGATATTC 120


15553R-1.IR_full       121 CAGTACAATGAAACCGATTGTAAGCTATTGGATAGCAAAAACACCACCTCAGAAATACAG 180
                           |||||||||||||||||||||||||||||||||||||||||||||||| |||||||||||
15553R-1.in silico     121 CAGTACAATGAAACCGATTGTAAGCTATTGGATAGCAAAAACACCACCACAGAAATACAG 180


15553R-1.IR_full       181 GCCATTGAGACGGAGCTTCAGGATTACGTGGCTAACATGTTTCTAACCCGCACCCTCTTC 240
                           || ||||||||||||||||||||||||||||||||||||||| |||||||||||||||||
15553R-1.in silico     181 GCAATTGAGACGGAGCTTCAGGATTACGTGGCTAACATGTTTTTAACCCGCACCCTCTTC 240


15553R-1.IR_full       241 GAAAGTATTATGCCCGCCATCTGTGGCTTATTCGTTGGATCCTGGTCGGATCAGTATGGA 300
                           |||||||||||||| |||||||||||||||||||||||||||||||||||||||||||||
15553R-1.in silico     241 GAAAGTATTATGCCTGCCATCTGTGGCTTATTCGTTGGATCCTGGTCGGATCAGTATGGA 300


15553R-1.IR_full       301 CGCAAGCCCCTGATGATTGTCTCCCTGGTCGGCTTTTCCGCTTCTGCCCTAATCTCGTCT 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15553R-1.in silico     301 CGCAAGCCCCTGATGATTGTCTCCCTGGTCGGCTTTTCCGCTTCTGCCCTAATCTCGTCT 360


15553R-1.IR_full       361 ATCATTTGCTGGCTGTCCAGCTACTATATGGTTAATCCCTGGTGGTACACCTTGGCAGCC 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15553R-1.in silico     361 ATCATTTGCTGGCTGTCCAGCTACTATATGGTTAATCCCTGGTGGTACACCTTGGCAGCC 420


15553R-1.IR_full       421 GTGCCACACTCTGTGCTCGGCGGATGGTGTGTATTTTCTGTGGCTGCATTCTGCTTTATC 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15553R-1.in silico     421 GTGCCACACTCTGTGCTCGGCGGATGGTGTGTATTTTCTGTGGCTGCATTCTGCTTTATC 480


15553R-1.IR_full       481 ACGGACACAACGGACATGAA 500
                           ||||||||||||||||||||
15553R-1.in silico     481 ACGGACACAACGGACATGAA 500



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  2  NM_143584.2  CG15553-RA (CG15553), mRNA 
0.2   1  4  3  3  NM_169985.1  CG31465-RA (CG31465), mRNA 
0   0  21  47  51  NM_136620.2  CG8008-RA (CG8008), mRNA 
0   0  3  4  4  NM_140564.1  CG17029-RA (CG17029), mRNA 
0   0  2  9  15  NM_136621.2  CG8046-RB, transcript variant B (CG8046), mRNA 
0   0  2  9  15  NM_165657.1  CG8046-RA, transcript variant A (CG8046), mRNA 
0   0  2  3  2  NM_140190.1  CG14142-RA (CG14142), mRNA 
0   0  1  4  6  NM_001042993.1  CG17082-RB, transcript variant B (CG17082), mRNA 
0   0  1  4  6  NM_001042990.1  CG17082-RC, transcript variant C (CG17082), mRNA 
0   0  1  4  6  NM_001042992.1  CG17082-RD, transcript variant D (CG17082), mRNA 
0   0  1  4  6  NM_001042994.1  CG17082-RE, transcript variant E (CG17082), mRNA 
0   0  1  4  6  NM_001042989.1  CG17082-RA, transcript variant A (CG17082), mRNA 
0   0  1  4  6  NM_001042991.1  CG17082-RF, transcript variant F (CG17082), mRNA 
0   0  1  4  3  NM_164491.2  CG17302-RA (CG17302), mRNA 
0   0  1  3  3  NM_166294.1  CG30119-RA (CG30119), mRNA 
0   0  1  2  5  NM_142071.2  CG3061-RA (CG3061), mRNA 
0   0  1  2  3  NM_135181.1  CG13982-RA (CG13982), mRNA 
0   0  1  2  3  NM_138194.2  CG17090-RA, transcript variant A (CG17090), mRNA 
0   0  1  2  2  NM_079939.2  CG5580-RA, transcript variant A (sbb), mRNA 
0   0  1  2  2  NM_078536.3  CG12154-RA, transcript variant A (oc), mRNA 
0   0  1  2  2  NM_001014727.1  CG12154-RB, transcript variant B (oc), mRNA 
0   0  0  6  3  NM_134481.1  CG14220-RA (CG14220), mRNA 
0   0  0  4  4  NM_166163.1  CG8048-RC, transcript variant C (Vha44), mRNA 
0   0  0  4  4  NM_166164.2  CG8048-RD, transcript variant D (Vha44), mRNA 
0   0  0  4  3  NM_141002.1  CG11451-RA (CG11451), mRNA 
0   0  0  4  3  NM_137861.2  CG12190-RA (CG12190), mRNA 
0   0  0  4  2  NM_176777.1  CG14616-RC, transcript variant C (l(1)G0196), mRNA 
0   0  0  4  2  NM_176778.1  CG14616-RD, transcript variant D (l(1)G0196), mRNA 
0   0  0  4  2  NM_134313.2  CG8048-RB, transcript variant B (Vha44), mRNA 
0   0  0  4  2  NM_057918.3  CG8048-RA, transcript variant A (Vha44), mRNA 

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